The genetic differences among Luffa acutangula （L.） Roxb., Luffa cylindnca （L.） Roem. and their F1 hybrid and F2 population were analyzed by ISSR technolo- gy. The results showed that the genomic DNA of test mater...The genetic differences among Luffa acutangula （L.） Roxb., Luffa cylindnca （L.） Roem. and their F1 hybrid and F2 population were analyzed by ISSR technolo- gy. The results showed that the genomic DNA of test materials showed a high diversity; the genetic similarity coefficient between both parents was only 0.395; the bands of F1 hybrid mainly consisted of both parents＇; neither FI nor F2 was inherit- ed singly from male or female parent; there was greater genetic variation among F2 population.展开更多
The molecular structure of a higher plant myosin with two 174 kD heavy chains purified from the tendrils of Luffa cylindrica (L.) Roem. was viewed by electron microscopy. The myosin exhibited actin_activated MgATP...The molecular structure of a higher plant myosin with two 174 kD heavy chains purified from the tendrils of Luffa cylindrica (L.) Roem. was viewed by electron microscopy. The myosin exhibited actin_activated MgATPase activity and could be recognized immunologically by a monoclonal antibody against the skeletal muscle myosin. Electron micrographs of rotary shadowed images of this protein revealed that it had two heads with size and shape similar to those of the skeletal muscle myosin and a relatively short tail in comparison with the conventional myosin. Luffa tendril actin filaments were also visualized and occasionally other Luffa myosin_like proteins with globular structure at the tail ends were also observed. The structural similarity and immunological cross reactivity with antibodies against muscle myosin demonstrate that the 174 kD Luffa tendril myosin is a double_headed myosin. The possible involvement of myosin_actin interactions in Luffa tendril contact coiling will be the subject of further research.展开更多
A new pentacyclic triterpenoid saponin, named lucyoside Q, was isolated from the leaves of Luffa cylindrica. Its structure was established as 21 β hydroxyloleanolic acid 28 O β D glucopyranoside by means of th...A new pentacyclic triterpenoid saponin, named lucyoside Q, was isolated from the leaves of Luffa cylindrica. Its structure was established as 21 β hydroxyloleanolic acid 28 O β D glucopyranoside by means of the spectral evidence and chemical methods.展开更多
Objective:To assess the effect of short-term gavage of ethanolic extract of Imperata cylindrica L root on the ovarian activity and estrus behavior of female mice.Methods:Eighteen virgin female ddY mice,8 to 10 weeks o...Objective:To assess the effect of short-term gavage of ethanolic extract of Imperata cylindrica L root on the ovarian activity and estrus behavior of female mice.Methods:Eighteen virgin female ddY mice,8 to 10 weeks of age,weighing 22-25 g with regular estrus cycle,were divided into three groups.Group 1 received 0.5%carboxymethylcellulose,whereas Groups 2 and 3 received the ethanolic extract of Imperata cylindrica L root at 90 and 115 mg/kg body weight(b.w.)per day by gavage for 20 days,respectively.All of the groups were checked before 9 a.m.daily for vaginal cytology to determine the estrus phase.On day 21,the mice were sacrificed to collect serum samples to quantify the concentrations of reproductive hormones using enzyme-linked immunosorbent assay and to determine changes in the reproductive organs based on their reproductive organ weight,histomorphology,and histomorphometry of ovarium and uterus.Results:The reproductive organ weight in the treatment groups was similar compared with that in the control group.The 90 mg/kg b.w.treatment group showed an increase in corpus luteum number when compared with the control group,with few degenerated follicles and diminished oocytes.Moreover,the 115 mg/kg b.w.treatment group showed fewer primordial and primary follicles and an increase in corpus luteum number and a prolonged diestrus phase compared to the control and 90 mg/kg b.w.treatment groups.The histomorphology examination of the uterus showed that the thickness of myometrium and epithelium in the treated animals was similar to the control group.In addition,there was a significant decrease in follicle-stimulating hormone level in the 115 mg/kg b.w.treatment group(P<0.05).Conclusions:Short-term gavage of ethanolic extract of Imperata cylindrica L root reduces the follicle-stimulating hormone serum level and folliculogenesis.展开更多
The influence of cellulose nano fibers extracted from the fruit of luffa cylindrica (LC) on the tensile, flexural and impact properties of composite materials using poly lactic acid (PLA) processed by micro compoundin...The influence of cellulose nano fibers extracted from the fruit of luffa cylindrica (LC) on the tensile, flexural and impact properties of composite materials using poly lactic acid (PLA) processed by micro compounding and injection molding was studied. Preliminary results suggested promising mechanical properties. The impact strength, tensile strength and flexural strength of the composites increased with incorporation of very low content of LC fiber up to 2 wt%. But when the wt of LC fiber in the composite increased (5 wt% and 10 wt%), mechanical strength of the composites reduced probably due to agglomeration of cellulose fibers. However, modulus of composites was enhanced with increase in wt of fiber content in the composites. Before reinforcement, the LC fibers were modified with calcium phosphate in order to explore the possibilities of using these composites in biomedical industries. The novelty of this work is that there is no use of compatiblizer and coupling agent during the processing so that the cost of processing is reduced.展开更多
Silicon deposition in leaf trichome of six horticultural Cucurbitaceae species, cucumber (Cucumis sativus), pumpkin (Cucurbita maxima), melon (Cucumis melo), watermelon (Citrullus lanatus), sponge gourd (Luffa cylindr...Silicon deposition in leaf trichome of six horticultural Cucurbitaceae species, cucumber (Cucumis sativus), pumpkin (Cucurbita maxima), melon (Cucumis melo), watermelon (Citrullus lanatus), sponge gourd (Luffa cylindrica) and bottle gourd (Lagenaria siceraria var. hispida) was observed by an X-ray microanalyzer coupled with an environmental scanning electron microscope. The elements that presented in the surface of three or four leaves of the individual species were detected and mapped by the X-ray microanalyzer. In leaves of cucumber, pumpkin, and melon, high accumulation of silicon was detected in cells surrounding the bases of the trichome hair and the hair itself deposited calcium. On the other hand, in sponge gourd and bottle gourd, high accumulation of silicon was detected only in the hair. In watermelon leaves, silicon deposited both in the hair and in cells surrounding the bases of the hair. Thus, horticultural Cucurbitaceae plants have interspecific variation in the pattern of silicon deposition in leaf trichomes.展开更多
基金Supported by Jiangsu Province Science and Technology Support Program,China(BE2012323)Jiangsu Agricultural Science and Technology Innovation Fund[CX(11)1002]~~
文摘The genetic differences among Luffa acutangula （L.） Roxb., Luffa cylindnca （L.） Roem. and their F1 hybrid and F2 population were analyzed by ISSR technolo- gy. The results showed that the genomic DNA of test materials showed a high diversity; the genetic similarity coefficient between both parents was only 0.395; the bands of F1 hybrid mainly consisted of both parents＇; neither FI nor F2 was inherit- ed singly from male or female parent; there was greater genetic variation among F2 population.
文摘The molecular structure of a higher plant myosin with two 174 kD heavy chains purified from the tendrils of Luffa cylindrica (L.) Roem. was viewed by electron microscopy. The myosin exhibited actin_activated MgATPase activity and could be recognized immunologically by a monoclonal antibody against the skeletal muscle myosin. Electron micrographs of rotary shadowed images of this protein revealed that it had two heads with size and shape similar to those of the skeletal muscle myosin and a relatively short tail in comparison with the conventional myosin. Luffa tendril actin filaments were also visualized and occasionally other Luffa myosin_like proteins with globular structure at the tail ends were also observed. The structural similarity and immunological cross reactivity with antibodies against muscle myosin demonstrate that the 174 kD Luffa tendril myosin is a double_headed myosin. The possible involvement of myosin_actin interactions in Luffa tendril contact coiling will be the subject of further research.
文摘A new pentacyclic triterpenoid saponin, named lucyoside Q, was isolated from the leaves of Luffa cylindrica. Its structure was established as 21 β hydroxyloleanolic acid 28 O β D glucopyranoside by means of the spectral evidence and chemical methods.
文摘Objective:To assess the effect of short-term gavage of ethanolic extract of Imperata cylindrica L root on the ovarian activity and estrus behavior of female mice.Methods:Eighteen virgin female ddY mice,8 to 10 weeks of age,weighing 22-25 g with regular estrus cycle,were divided into three groups.Group 1 received 0.5%carboxymethylcellulose,whereas Groups 2 and 3 received the ethanolic extract of Imperata cylindrica L root at 90 and 115 mg/kg body weight(b.w.)per day by gavage for 20 days,respectively.All of the groups were checked before 9 a.m.daily for vaginal cytology to determine the estrus phase.On day 21,the mice were sacrificed to collect serum samples to quantify the concentrations of reproductive hormones using enzyme-linked immunosorbent assay and to determine changes in the reproductive organs based on their reproductive organ weight,histomorphology,and histomorphometry of ovarium and uterus.Results:The reproductive organ weight in the treatment groups was similar compared with that in the control group.The 90 mg/kg b.w.treatment group showed an increase in corpus luteum number when compared with the control group,with few degenerated follicles and diminished oocytes.Moreover,the 115 mg/kg b.w.treatment group showed fewer primordial and primary follicles and an increase in corpus luteum number and a prolonged diestrus phase compared to the control and 90 mg/kg b.w.treatment groups.The histomorphology examination of the uterus showed that the thickness of myometrium and epithelium in the treated animals was similar to the control group.In addition,there was a significant decrease in follicle-stimulating hormone level in the 115 mg/kg b.w.treatment group(P<0.05).Conclusions:Short-term gavage of ethanolic extract of Imperata cylindrica L root reduces the follicle-stimulating hormone serum level and folliculogenesis.
文摘The influence of cellulose nano fibers extracted from the fruit of luffa cylindrica (LC) on the tensile, flexural and impact properties of composite materials using poly lactic acid (PLA) processed by micro compounding and injection molding was studied. Preliminary results suggested promising mechanical properties. The impact strength, tensile strength and flexural strength of the composites increased with incorporation of very low content of LC fiber up to 2 wt%. But when the wt of LC fiber in the composite increased (5 wt% and 10 wt%), mechanical strength of the composites reduced probably due to agglomeration of cellulose fibers. However, modulus of composites was enhanced with increase in wt of fiber content in the composites. Before reinforcement, the LC fibers were modified with calcium phosphate in order to explore the possibilities of using these composites in biomedical industries. The novelty of this work is that there is no use of compatiblizer and coupling agent during the processing so that the cost of processing is reduced.
文摘Silicon deposition in leaf trichome of six horticultural Cucurbitaceae species, cucumber (Cucumis sativus), pumpkin (Cucurbita maxima), melon (Cucumis melo), watermelon (Citrullus lanatus), sponge gourd (Luffa cylindrica) and bottle gourd (Lagenaria siceraria var. hispida) was observed by an X-ray microanalyzer coupled with an environmental scanning electron microscope. The elements that presented in the surface of three or four leaves of the individual species were detected and mapped by the X-ray microanalyzer. In leaves of cucumber, pumpkin, and melon, high accumulation of silicon was detected in cells surrounding the bases of the trichome hair and the hair itself deposited calcium. On the other hand, in sponge gourd and bottle gourd, high accumulation of silicon was detected only in the hair. In watermelon leaves, silicon deposited both in the hair and in cells surrounding the bases of the hair. Thus, horticultural Cucurbitaceae plants have interspecific variation in the pattern of silicon deposition in leaf trichomes.
