Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant...Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.展开更多
The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser-. The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1% α-helix, 33.4% β-sheet,and 29.5% random coil. N-glyco...The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser-. The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1% α-helix, 33.4% β-sheet,and 29.5% random coil. N-glycosidase activity of Luffaculin 1 against animal rRNA is observed. The anti-tumor activity of Luffaculin 1 on cell strains B16, MGC, Bel were determined,giving IC50 of 1.78×10^-7mol/L,2.11×10^-7mol/L,and 4.21×10^-7mol/L,respectively. We also discussed the N-terminal sequences, secondary structures,and bioactivities of Luffaculin 1 against Trichosanthin.展开更多
基金This work was supported by the National Natural Science Foundation of China (No. 39970872) and Natural Science Foundation of Fujian Province (C97052)
文摘Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.
文摘The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser-. The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1% α-helix, 33.4% β-sheet,and 29.5% random coil. N-glycosidase activity of Luffaculin 1 against animal rRNA is observed. The anti-tumor activity of Luffaculin 1 on cell strains B16, MGC, Bel were determined,giving IC50 of 1.78×10^-7mol/L,2.11×10^-7mol/L,and 4.21×10^-7mol/L,respectively. We also discussed the N-terminal sequences, secondary structures,and bioactivities of Luffaculin 1 against Trichosanthin.
