Network Biological Modeling:A Novel Approach to Interpret the Traditional Chinese Medicine Theory of Exterior-Interior Correlation Between the Lung and Large Intestine

Objective To study the common pathogenesis of pneumonia and colitis using modern biological network analysis tools,and to explore the theory that the lung and large intestine are exteriorly and interiorly related.Methods The relevant target genes(hereinafter,“targets”)of pneumonia and colitis were separately queried on the GeneCards database.The main targets of the two diseases were then screened out according to their correlation scores and intersected to obtain those common to the two diseases.Metascape was used to analyze the main and common targets identified,and the Database for Annotation,Visualization and Integrated Discovery(DAVID)was used to enrich and analyze the common targets.Cytoscape 3.7.2 software was used to build the network diagram.Results In total,54 targets,such as TNF,IL-10,IL-6,IL-2,IL-4,TLR4,TLR2,CXCL8,IL-17A and IFNG,etc.,are common to pneumonia and colitis,which are mainly enriched in these processes such as cytokine–cytokine receptor interaction,the Tcell receptor signaling pathway,the Toll-like receptor signaling pathway and the Jak-STAT signaling pathway.The Metascape modular analysis identified 11 modules for pneumonia,six modules for colitis,and two modules for the common targets.Conclusions Pneumonia and colitis have the same pathogenic targets and mechanisms of action and finally interact with each other through inflammatory reactions and immune responses.This provides a probable molecular mechanism that explains the theory that the lung and large intestine are exteriorly and interiorly related.

Experimental Study on the Mechanism of Protective Effect of Free Fu on Gut-derived Endotoxin-Mediated Lung Damage

The effect of tumor necrosis factor-α (TNF-α) on endotoxin (ET)-mediated lung damage caused by incomplete ligation of large intestine and the influence of free Fu on the expression of TNF-α mRNA were explored. Forty SD rats were randomly divided into 4 groups: normal control group, model group, ligation group and treatment group (n=10 in each group). The models were made by the method of partly ligating the rectum outside the body. The plasma level of lipopolysaccaride was measured by dynamic nephelo metric method and the serum level of TNF-α was detected by the method of radioactive immunity. The expression of TNF-α mRNA in lung tissue was detected by RT-PCR method. The results were compared among the 4 groups. The results showed the plasma levels of ET and serum TNF-α in the model group and the expression of TNF-α mRNA in the lung tissues were remarkably higher than those in the normal control group (P<0.01). After the treatment of free Fu, all of the above indexes in the treatment group were all decreased as compared with model group (all P<0.01), and the damage to lung was alleviated. It was concluded that TNF-α might play a very important role in the ET-mediated lung damage caused by incomplete ligation of large intestine, free Fu could protect the lung from damage.

A Novel Apprehension of the Primary Lung Meridian, Sinew Channel, Divergent Channel, Luo-Connecting Channel Acting as a Single Unit System to Serve Respiration Function Based on Modern Neurophysiology and Kinesiology

The TCM philosophy of a meridian and associated channels pertains to the specific function of one or more organs. We define the Lung Primary Meridian (LUM) together with the Lung Sinew (LUSC), Divergent (LUDC), Luo-connecting (LULCC) Channels as a system of routes plus some parts of the body (such as muscles) to fulfil respiration, as a main function under different situations. There is very limited information about the Lung associated channels in classical literature of TCM. With a clear focus on the function of respiration, we have carried out a detailed analysis of the biomedical consequence of stimulating the LUM, analysed the roles played by LUSC, LUDC, and LULCC. The updated LUM and LUDC include acupoints of other meridians, serving the same purpose of performing satisfactory respiration starting from checking the quality of the inflow through the nose. The LUSC includes the respiratory muscles (plus the associated connective tissues) extending to various parts of the body. The muscles of the limb (as part of the LUSC) embrace the nerves that provide routes for somatosensory reflexes and play the role of locomotion, providing voluntary respiration via the pectoralis muscles. The muscles of LUSC are bounded by stiff connective tissue layers, forming compartments, and are part of the pulley system for various body locomotions. Within a compartment, the interstitial fluid, blood, lymph flows must be potent to protect the associated nerves related to LUM;the healthy state of the LUSC also provides freedom of various types of locomotion. The LULCC exists because the vagus nerve has a part of it passing through the spinal cords all the way down to the sacrum domain, with exiting nerve innervating two-third of the large intestine. The crucial steps of our deductions are supported by experimental evidence based on modern neurophysiology and kinesiology. We discover that all the four channels stated above work as a unit system to allow respiration to be possible under various postures/conditions. The complexity of structures and processes is eased off by providing 29 figures and 13 tables for the relevant muscles and nerves. In addition to respiration, the Lung system in TCM context includes interaction of this system with the sweat gland and neuroendocrine system;such aspects will be left to another study.

基于“肺与大肠相表里”探讨IgA肾病的机制与治疗

IgA肾病(IgA nephropathy,IgAN)是全世界最常见的原发性肾小球疾病,是导致终末期肾病的常见原因。目前尚难解释其病因及发病机制,随着研究的深入,众多学者发现肠道菌群失调及黏膜免疫反应与该病的发生发展紧密相关。而呼吸道感染导致并加重IgA肾病的临床进展不容忽视。试从“肺与大肠相表里”的中医经典理论结合现代医学研究,综述分析肺、肠与IgAN的关系,以期思考药物基于“肺与大肠相表里”理论应用于IgAN的可能防治机制,为IgAN的临床治疗及研究提供新方向。

参苓白术散治疗大肠相关性疾病的研究进展

参苓白术散的源自于《太平惠民和剂局方》,是渗湿止泻、培土生金的典型代表方。其主治脾虚湿盛导致的泄泻、呕吐等疾病。由于其在临床上表现出显著的疗效,因此被广泛用于治疗各种内伤外感杂病。近年来,随着溃疡性结肠炎、腹泻型肠易激综合征、功能性腹泻等大肠相关性疾病的发病率逐年上升,患者的生活质量和身心健康受到了严重影响。虽然有关参苓白术散治疗这些疾病的研究取得了显著成果,但由于缺乏对相关文献的整理,该方的治疗价值未能及时挖掘和利用。文章通过对近年来参苓白术散治疗大肠性相关疾病的理论基础以及临床应用的综述,试图全面了解该方的应用及疗效。近年来的文献梳理显示,参苓白术散在治疗大肠相关性疾病(包括腹泻型肠易激综合征、溃疡性结肠炎、功能性腹泻、克罗恩病、结直肠癌)方面表现出显著的疗效,包括改善临床症状、降低复发率、减少不良反应等优势。通过整理相关文献,对参苓白术散治疗大肠相关性疾病的理论基础以及临床研究进行了综合分析,旨在为进一步提高其在防治大肠性相关疾病的研究提供参考依据。

Study on Traditional Chinese Medicine theory of lung being connected with large intestine

OBJECTIVE:The theory of lung being connected with large intestine,which is a major topic in Traditional Chinese Medicine(TCM),has guided clinical practice for thousands of years in China.METHODS:In this study,we analyzed the history,main contents,clinical application,and material basis of the theory,to attempt to improve the potential clinical significance of "lung being connected with large intestine" in China.RESULTS:The lung being connected with large intestine was first described in "Huang Di Nei Jing",and formed one of the basic theories of TCM.For thousands of years,the majority of TCM practitioners explored this theory continuously,leading to its development and use as an important theory in the guidance of TCM clinics In the last decade,researchers in the field of integrated TCM and Western medicine have studied clinical applications and biomedical mechanisms with experimental methods to explore the implications of the theory.CONCLUSION:With the further development of science and technology,research concerning the theory of lung being connected with large intestine will be greatly stimulated and contribute to the modernization of TCM.

The influence of intestine-based treatment using Xuan Bai Cheng Qi Tang on the concentration of trace elements in the main organs of COPD rats

Objective:To test two theories from traditional Chinese medicine:'exterior -interior relationship between the lung and large intestine' and 'treating from the intestine principle for lung disorders'.The influence of intestine-based treatment using Xuan Bai Cheng Qi Tang (XBCQT) on the concentration of three trace elements-copper (Cu),zinc (Zn),and manganese (Mn)-was observed in the tissues of the lung,small intestine,large intestine,and stomach of rats suffering from chronic obstructive pulmonary disease (COPD).Methods:Thirty-five male Wistar rats were divided randomly and equally into five groups:control;model;Fei treatment (A);Chang treatment (B);and Fei-Chang treatment (C).A rat model of COPD was prepared by tracheal injection of lipopolysaccharide plus exposure to cigarette smoke.Treatments with medicinal herbs started day-22 of administration and exposure to cigarette smoke for 7 days.The control group and model group were administered physiologic (0.9%) saline solution via the stomach.After 7 days of intervention,the tissues of the lung,small intestine,large intestine,and stomach were removed.Inductively coupled plasma-atomic emission spectroscopy was used to detect the levels of Cu,Zn,and Mn in those tissues.Results:Compared with the control group,the Cu concentration in the tissues of the small intestine,large intestine,and stomach increased significantly in the model group (P <.05);the Mn concentration in the tissues of the lung,large intestine,and stomach increased significantly in the model group (P <.05);the Zn concentration in the tissues of the lung and large intestine decreased significantly in the model group (P <.05).In comparison of the model group:the Cu concentration in the tissues of the lung and large intestine decreased significantly in the B group (P <.05);the Mn concentration in the tissues of the lung,small intestine,and large intestine decreased significantly in the B group (P <.05);the Zn concentration in the tissues of the lung,small intestine,and large intestine increased significantly in the B group (P <.05).For the A group versus C group comparison,the Zn concentration in the tissues of the small intestine and stomach increased significantly in the latter (P <.05).Conclusion:This study showed that 'treating from the intestine' using Xuan Bai Cheng Qi Tang and its modified formulae can regulate the concentration of trace elements in the main organs of COPD rats.This may be one of the mechanisms for intestine-based treatment for COPD.

Discussion on the clinical application of colorectal cancer based on the theory of "Tongqixiangqiu"

Objective:To explore the application of the theory of"Tongqixiangqiu"and its application in traditional Chinese medicine in order to find out the clinical application of colorectal cancer.Methods:Referring to the ancient medical works and modern literature on the theory of"Tongqixiangqiu",combined with the disease characteristics of colorectal cancer and the clinical experience of famous doctors in ancient and modern times,to explore its application in the clinical treatment of colorectal cancer.Results:The theory of"Tongqixiangqiu"has rich connotations,among which"anti-adjuvant therapy"and"lung and intestine combined treatment"are the representatives of the specific application of the theory.The methods of cold and heat counteraction,compatibility and external treatment as well as the idea of spleen deficiency and Qi stagnation type colorectal cancer pay attention to tonifying Fei qi,nourishing Fei yin,and promoting Sanjiao Qi mechanism play an important role in the differentiation and treatment of colorectal cancer in TCM.Conclusion:The theory of"Tongqixiangqiu"is widely used in the treatment of colorectal cancer,which has the advantages of significant curative effect,synergism and toxicity reduction,prolonging the survival period and improving the quality of life of patients.

杨燕教授从肺论治儿童功能性便秘的临床经验

儿童功能性便秘约占小儿便秘的95%以上,是小儿消化系统的常见病,病程迁延易对儿童的身心健康产生严重影响。文章总结杨燕教授治疗儿童功能性便秘的临床经验。杨燕教授认为便秘的病机主要是大肠的传导功能失常,但与肺脏密切相关。肺与大肠相表里,肺气亏虚或肺气郁闭,宣肃失职,气机不畅,津液疏布失常,肺热移于大肠,肠失濡润,均可导致便秘发生。从肺论治,临证采用塞因塞用之法补气启闭治虚秘,提壶揭盖之法理肺通腑治实秘,同时重视良好的饮食结构及日常护理,临床疗效显著。附验案1则以佐证。

健脾祛湿和络方治疗特发性膜性肾病内涵探讨

从多维论治角度结合部分健脾祛湿和络方的研究进展,阐释健脾祛湿和络方有效治疗特发性膜性肾病(IMN)的中医学内涵。IMN以脾虚证、肾虚证为核心证候,健脾祛湿和络方立方以调理脾胃为基础,脾肾同治。从肺肾相关、肠肾相关、肺与大肠相表里等理论看,健脾祛湿和络方亦体现出了脾肺同治、肠肾同治、肠肺同治的中医学内涵,脾、肺、肠、肾相结合的多维论治是健脾祛湿和络方取效之根本。

从“金郁泄之”论治溃疡性结肠炎

“金郁泄之”理论出自《黄帝内经》,“金”是肺与肠的基本属性,“郁”指郁滞,壅塞不通的异常状态,故“金郁”可理解为属金脏腑如肺、大肠所发壅滞、阻塞之证。“泄之”是五行属金脏腑郁滞的治疗方法,其具体含义应随“金郁”的病理产物、病位的不同而不同。溃疡性结肠炎多由邪蕴肠腑,气血瘀滞,肠道传导失司,湿、热、瘀于大肠相互搏结成郁,改变肠道属金肃降之性所致,其核心病机为湿热内蕴,气血瘀滞,属于“金郁”的范畴,病位虽在大肠,可波及于肺。故治疗应顺应“泄之”的法则,以祛除肺肠之郁为主,包括清泄与宣泄两种方法,同时补益肺、脾、肾三脏之气,以恢复肺肠之功能,达到扶正祛邪,标本兼顾的目的,促进溃疡性结肠炎的愈合。

基于“肺与大肠相表里”探析血管内皮细胞与肠道菌群的关联

肠道菌群及其代谢物失调对内皮细胞损伤和功能障碍具有重要影响。中医“肺”与血管内皮细胞在结构、生理及病理方面具有相关性。中医学“肺与大肠相表里”理论是肺与肠在结构、生理及病理方面相关性的高度概括,可作为血管内皮细胞与肠道菌群相关联的理论基础。本文可为以肠道菌群为切入点、修复血管内皮细胞损伤干预心血管疾病的实验研究提供理论依据,并为中医药临床治疗相关疾病提供参考。

基于肺与大肠相表里理论探讨呼吸训练改善脑卒中患者便秘的临床疗效

[目的]基于“肺与大肠相表里”理论观察呼吸训练改善脑卒中便秘患者的临床疗效,进一步扩展其理论应用。[方法]将66例脑卒中便秘患者按照随机数字表法分为两组,对照组32例(脱落1例)予乳果糖口服液治疗,治疗组33例在对照组治疗基础上加用呼吸训练。两组均治疗3周,评估并比较两组慢性便秘严重程度评分、膈肌移动度和腹肌增厚率。[结果]治疗后两组的便秘程度均出现不同程度的降低,治疗后呼吸训练组的慢性便秘严重程度评分量表得分低于对照组,差异具有统计学意义(P<0.05)。相关性分析显示,腹内斜肌增厚率、膈肌移动度与频率、费力程度、排便不尽感频率、每次如厕时间和便秘程度等5个指标间均具有显著正相关;此外,膈肌移动度与辅助排便方法间呈现显著正相关,差异均具有统计学意义(P<0.05)。[结论]呼吸训练明显改善脑卒中便秘患者临床疗效,同时明显改善患者膈肌、腹肌功能,进一步拓展了肺与大肠相表里藏象理论临床应用。

从“肺与大肠相表里”阐释针灸治疗哮喘的理论内涵

针灸治疗肺系疾病有深厚的理论根源和得天独厚的优势,并有丰富的临床应用经验。“肺与大肠相表里”理论指导下针灸“肺肠同治”对改善哮喘症状及气道炎症反应具有重要意义。基于“肺与大肠相表里”理论,主要从肺与大肠组织相连、经络相通、功能上相互为用以及病理上的相互影响等方面,系统阐释肺与大肠的特殊关系,进而阐释“肺肠同治”针灸治疗哮喘的理论内涵和意义,为临床“肺肠同治”针灸治疗哮喘的推广和应用提供理论基础,更好地指导临床实践。

从“肝与大肠相通”理论辨治代谢相关脂肪性肝病的应用与中医实践

代谢相关脂肪性肝病(MAFLD)是一种除酒精或其他明确肝损伤因素导致的肝脏病理综合征,随着近年饮食及生活习惯的改变,MAFLD的流行率以前所未有的速度上升,已成为我国慢性肝病及肝酶异常的第一大原因。MAFLD具有起病隐匿、发病率高及肝外不良影响等特点,发病与胰岛素抵抗、脂质代谢紊乱等因素密切相关。中医学将MAFLD命名为“肝癖”,认为因肝体用失调、脾肾亏虚等,最终出现痰瘀互结,阻滞肝脏经络最终导致MAFLD。“肝与大肠相通”理论是目前治疗MAFLD的新思路,通畅肠腑、降浊升清,可协调肠腑、肝脏气机,从而辅助提高MAFLD治疗效果。遂从“肝与大肠相通”理论的内涵、应用及医案举例等入手,剖析“肝与大肠相通”理论,为临床治疗MAFLD提供一种新的治疗思路。

基于“肺-肠轴”交互作用探讨宣白承气汤在肺系疫病治疗中的意义

肺系疫病是由外感疫毒引起的急性呼吸道传染病,消化道症状为其常见兼证被视为疾病的传变。“肺-肠轴”交互作用与中医“肺与大肠相表里”理论具有一致性,均阐释了肺与大肠的协调与统一。医家多认为肺系疫病的治疗应从肺肠同治的角度出发,宣肺以畅达气机,泻下以通调水道。本文通过分析古代与现代医家对肺系疫病的研究以及患者的症状,基于“肺与大肠相表里”理论,从宣肺通腑的角度出发,探讨宣白承气汤肺肠同治原则在肺系疫病治疗中的意义,给肺系疫病以新的诊疗思路,提供新的治疗途径。

基于“肺与大肠相表里”理论和“整体观念”探讨慢性肺系病合并功能性便秘的诊治思路

慢性肺系病和功能性便秘均是临床常见的难治性疾病,且易合并出现。对二者分别进行干预是现代医学的常规治疗模式,但疗效及安全性均不能令人满意。课题组在临床实践中发现,中医药治疗慢性肺系病合并功能性便秘具有独特的优势。笔者在中医“肺与大肠相表里”理论和“整体观念”指导下,提出慢性肺系疾病合并功能性便秘的病机涉及肺失宣肃致腑气不通、大肠传导失司致肺气上逆、脾失健运、肾精亏损、气血两虚、久病瘀滞等方面,治疗当以“肺肠合治”为总则,辅以健脾益肾、补气养血以扶本虚,行气化瘀以祛实邪,并据此整理临床常用方药,系统总结了本病的中医诊治思路。

基于“肺合大肠”理论探讨肺纤维化中医病机

肺纤维化是一种以肺泡上皮细胞损伤、成纤维细胞病理性增殖转型、细胞外基质过度沉积导致肺组织结构破坏及功能丧失的呼吸系统疾病,病死率高且尚无较好治疗方法。本文以“肺合大肠”的中医认识即“肺与大肠相表里”理论为基础,以“肺合大肠”的现代医学认识即“肠-肺轴”理论为关键点,结合二者探讨相关机制,初步阐述中医药调控“肠-肺轴”治疗肺纤维化的内涵,为临床通过“肠-肺轴”治疗肺纤维化提供新思路。

从“肺-肠”轴角度探讨肠道菌群与过敏性鼻炎的关系

过敏性鼻炎属于全球高发的气道变应性疾病,较多人群深受其影响,关于其更安全有效的治疗方式仍不断被探寻与挖掘。近年来关于肠道微生态与肺部疾病的相互关系被深入研究,文章基于肺肠关系,结合中医“肺与大肠相表里”理论,并从西医胚胎发育、菌群微环境、内分泌代谢、黏膜免疫等角度探讨肺-肠关系,在此基础上,认为肠道菌群可从细胞因子免疫平衡、机体黏膜等方面影响过敏性鼻炎的发生发展,以期为临床治疗过敏性鼻炎提供更多思路。

从“肺合大肠”探讨肠道菌群紊乱与脓毒症急性肺损伤的相关性

脓毒症急性肺损伤发病机制复杂、死亡率高,目前尚缺乏有针对性的治疗手段。肠道菌群紊乱可通过“肠-肺轴”参与急性肺损伤发生发展。通过肠道菌群这一现代医学认识来阐释中医学“肺合大肠”理论,基于“肺合大肠”探讨肠道菌群紊乱在急性肺损伤中医发病机制中的作用,以“肺肠同治”为基本原则,提出以改善肠道生态平衡为核心的急性肺损伤防治新策略,明确分期论治:初期邪在卫表,肺气郁闭,肠络受损,治疗以清热宣肺、润下通腑为主,佐以理气机、消痈滞;中期邪炽气营,肺络瘀阻,肠腑失通,治疗以解毒清肺、攻下通腑兼以凉血化瘀;后期血分热极,肺阴亏耗,津枯肠结,逆证丛生,治以养阴透热、通腑开闭佐以扶正救逆。