Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic h...Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic human lung carcinoma cell line, by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) cell viability assay, and to study the change of intracellular calcium concentration, connexin43 (Cx43) expression, gap junctional intercellular communication (GJIC) and cell cycle distribution after the tea polyphenol treatment by laser scanning confocal microscopy and flow cytometry. The results showed that 1) tea polyphenol could kill the PG cells in a dose-depent manner via inhibiting the PG cell proliferation and blocking the PG cell cycle progression staying in G0/G1 phase and not transfering in S and G2/M phases to reduce the PG cell proliferation index;2) the increases of intracellular calcium concentration, GJIC and Cx43 expression were related with the tea polyphenol doses. The data suggested that tea polyphenol could inhibit the growth of PG cells, which mechanism was associated with the up-regulation of GJIC.展开更多
In recent years,as we have a better knowledge and understanding of the biology of non small cell lung carcinoma(NSCLC),which leads us to targeting biomarkers driving the NSCLC carcinogenesis and metastatic potential,w...In recent years,as we have a better knowledge and understanding of the biology of non small cell lung carcinoma(NSCLC),which leads us to targeting biomarkers driving the NSCLC carcinogenesis and metastatic potential,we now have an increased number of options to offer our patients with NSCLC.We also realize the importance of distinguishing squamous and non squamous histology to guide our treatment decisions of NSCLC.The palliative care concomitant with therapies from the very start of the treatment also showed an impact on survival.This review examines the treatment options in all lines of therapy for metastatic NSCLC that have been approved in Canada,the United States,or Europe.展开更多
Aim:Murine tumour cell lines have been used in thousands of studies.Strikingly,it is rather the rule than exception for most of them that not much is known about their genetic characteristics.The squamous cell carcino...Aim:Murine tumour cell lines have been used in thousands of studies.Strikingly,it is rather the rule than exception for most of them that not much is known about their genetic characteristics.The squamous cell carcinoma(SCC)cell line KLN 205 is such an example.KLN 205 cells have not been studied yet for karyotype or acquired copy number variations(CNVs),but they have been used as models for(metastatic)lung cancer,lung-SCC,non-small cell lung cancer,tongue cancer and subcutaneous SCC.Here,it was characterised cytogenomically for the first time.Methods:The cell line KLN 205 was characterised comprehensively by molecular cytogenetics using multicolour banding as well as molecular karyotyping.Based on these results,a map of the imbalances and breakpoints determined in the murine genome was translated to the human genome.Results:Here,it could be shown that this>40-year-old cell line has a stable,approximately tetraploid karyotype comprising 77-82 chromosomes.However,there are few structural chromosomal aberrations:only six derivatives involving chromosomes 2,3,5,9,10 and/or 19 could be found.According to the literature,SCCs derived from different human tissues,as well as lung SCC and non-small cell lung cancer,display overall similar CNV patterns.Conclusion:Thus,according to the genetic profile found here,KLN 205 can be applied as a general model for human SCC;it is also suited as a model for lung cancer in general.Further molecular genetic characterisation of KLN 205 cell line may find more lung-and/or SCC-specific alterations.展开更多
文摘Our previous study has proven that tea polyphenol has a role in lung neoplasms. The present communication was to investage the anti-proliferation effect of tea polyphenol on the PG cells, which was a high metastatic human lung carcinoma cell line, by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) cell viability assay, and to study the change of intracellular calcium concentration, connexin43 (Cx43) expression, gap junctional intercellular communication (GJIC) and cell cycle distribution after the tea polyphenol treatment by laser scanning confocal microscopy and flow cytometry. The results showed that 1) tea polyphenol could kill the PG cells in a dose-depent manner via inhibiting the PG cell proliferation and blocking the PG cell cycle progression staying in G0/G1 phase and not transfering in S and G2/M phases to reduce the PG cell proliferation index;2) the increases of intracellular calcium concentration, GJIC and Cx43 expression were related with the tea polyphenol doses. The data suggested that tea polyphenol could inhibit the growth of PG cells, which mechanism was associated with the up-regulation of GJIC.
文摘In recent years,as we have a better knowledge and understanding of the biology of non small cell lung carcinoma(NSCLC),which leads us to targeting biomarkers driving the NSCLC carcinogenesis and metastatic potential,we now have an increased number of options to offer our patients with NSCLC.We also realize the importance of distinguishing squamous and non squamous histology to guide our treatment decisions of NSCLC.The palliative care concomitant with therapies from the very start of the treatment also showed an impact on survival.This review examines the treatment options in all lines of therapy for metastatic NSCLC that have been approved in Canada,the United States,or Europe.
基金supported by grant#2013.032.1 of the Wilhelm Sander-Stiftung.
文摘Aim:Murine tumour cell lines have been used in thousands of studies.Strikingly,it is rather the rule than exception for most of them that not much is known about their genetic characteristics.The squamous cell carcinoma(SCC)cell line KLN 205 is such an example.KLN 205 cells have not been studied yet for karyotype or acquired copy number variations(CNVs),but they have been used as models for(metastatic)lung cancer,lung-SCC,non-small cell lung cancer,tongue cancer and subcutaneous SCC.Here,it was characterised cytogenomically for the first time.Methods:The cell line KLN 205 was characterised comprehensively by molecular cytogenetics using multicolour banding as well as molecular karyotyping.Based on these results,a map of the imbalances and breakpoints determined in the murine genome was translated to the human genome.Results:Here,it could be shown that this>40-year-old cell line has a stable,approximately tetraploid karyotype comprising 77-82 chromosomes.However,there are few structural chromosomal aberrations:only six derivatives involving chromosomes 2,3,5,9,10 and/or 19 could be found.According to the literature,SCCs derived from different human tissues,as well as lung SCC and non-small cell lung cancer,display overall similar CNV patterns.Conclusion:Thus,according to the genetic profile found here,KLN 205 can be applied as a general model for human SCC;it is also suited as a model for lung cancer in general.Further molecular genetic characterisation of KLN 205 cell line may find more lung-and/or SCC-specific alterations.
