NORTHERN BLOT ANALYSIS OF nm23 GENE EXPRESSION IN HUMAN LUNG CANCER

Objective: To investigate the role of nm23 gene expression in human lung cancer. Methods: Forty human lung cancer tissues and 19 non-cancer pulmonary tissues were studied for their nm23-H1 and nm23-H2 mRNA expression with non-radioactive Northern blot hybridization. The correlation of nm23 mRNA expression with clinical features of lung cancer was analyzed. Results: The mRNA expression of nm23-H2 gene in poorly differentiated squamous cell carcinoma was significantly decreased compared to that in moderate-high differentiated squamousd cell carcinoma. The mRNA expression of nm23-H1 and nm23-H2 gene in small cell lung cancer was significantly decreased compared to that in squamous cell carcinoma. No significant difference in nm23 mRNA expression was observed between lung cancer with and without lymph node metastasis, nor was there significant difference between tumor stage. Conclusion: The mRNA expression of nm23 gene is correlated with the degree of differentiation of lung cancer, but there is no evidence of metastasis suppression effect by nm23 gene.

PKC基因在人肺癌中的转录表达

目的 探讨PKC β1基因转录表达与肺癌临床病理生理特征的关系。方法 应用Northern印迹杂交方法检测 5 0例肺癌组织、癌旁肺组织、远癌肺组织和 30例肺良性病变肺组织PKC β1mRNA表达水平。结果 (1)肺癌组织中PKC β1mRNA表达水平显著高于癌旁肺组织 ,远癌肺组织和肺良性病变肺组织 (P <0 .0 1) ;(2 )低分化肺癌中PKC β1表达水平显著高于中 高分化肺癌 (P <0 .0 5 ) ;(3)Ⅲ期肺癌PKC β1表达水平显著高于Ⅰ、Ⅱ期肺癌 (P <0 .0 1) ;(4)伴有淋巴结转移肺癌PKC β1表达水平显著高于不伴淋巴结转移肺癌(P <0 .0 1)。结论 肺癌组织中存在PKC β1mRNA过度表达 ,它可能参与肺癌细胞分化以及肿瘤进展和转移过程的调控。

Northern印迹杂交分析nm23基因在人肺癌中的表达研究

目的探讨ｎｍ２３基因表达在人肺癌中的作用。方法通过Ｎｏｒｔｈｅｒｎ印迹杂交，检测４０例人肺癌组织和１９例非癌肺组织的ｎｍ２３Ｈ１和ｎｍ２３Ｈ２ｍＲＮＡ表达，采用地高辛标记和检测系统显示杂交信号，并分析ｍＲＮＡ表达与肺癌临床特征的关系。结果低分化鳞癌的ｎｍ２３Ｈ２ｍＲＮＡ表达较中高分化鳞癌显著降低（Ｐ＜０．０１），小细胞肺癌的ｎｍ２３Ｈ１和ｎｍ２３Ｈ２ｍＲＮＡ表达较肺鳞癌明显降低。但在有或无淋巴结转移的原发癌灶组织间，以及肺癌的临床分期间，ｎｍ２３基因ｍＲＮＡ表达差异无显著性（Ｐ＞０．０５）。结论ｎｍ２３基因ｍＲＮＡ表达与肺癌的组织分化有关，未发现其在肺癌中的癌转移抑制作用。

Antisense expression of protein kinase Cα improved sensitivity to anticancerdrugs in human lung cancer LTEPa-2 cells

目的：研究蛋白激酶Ｃα（ＰＫＣα）在人肺癌ＬＴＥＰａ２细胞对一些临床抗肿瘤药物敏感性中的作用．方法：通过基因转染，免疫印迹等方法建立表达反义ＰＫＣα的人肺癌细胞模型，Ｎｏｒｔｈｅｒｎ印迹检测多药抗性基因的表达，分析了几种抗癌药物对培养细胞的ＩＣ５０．结果：表达反义ＰＫＣαＲＮＡ降低胞内ＰＫＣα水平时可抑制肺癌细胞中多药抗性基因的表达，增强肺癌细胞对抗肿瘤药物（三尖杉酯碱、卡铂、博来霉素、长春新碱、阿霉素）的敏感性．

Identification of metastasis associated gene G3BP by differential display in human cancer cell sublines with different metastatic potentials G3BP as highly expressed in non-metastatic

OBJECTIVE: To investigate genes involved in cancer metastasis, mRNA differential display was used to compare the levels of gene expression in two cell sublines derived from human giant cell carcinoma of lung (PG) which had different metastatic potentials. METHODS: Using in vivo tumorigenicity and a spontaneous metastasis assay in nude mice, two sublines (BE1, LH7) from human giant cell carcinoma of lung (PG) with different metastatic potentials were isolated and characterized. mRNA differential display was used to compare the levels of gene expression between them and the obtained results were confirmed by Northern hybridization. RESULTS: One differentially expressed band was nearly identical (99% homology) to Ras-GTPase-Activating protein SH3 domain binding protein (G3BP). G3BP displayed a strong expression in LH7 (non-metastatic in recipient nude mice) and a very weak expression in BE1 (100% metastatic frequency). The same different expression level of G3BP was detected in Northern hybridization with another panel of cell sublines with different metastatic potentials (established in our lab) derived from human prostate carcinoma cell line PC-3M. CONCLUSION: Our results indicate that G3BP was implicated in cancer metastasis because of its differential expressions in the two panels of cell sublines with different metastatic potentials.