Objective： To investigate the expression offragile histidine triad （FHIT） and p53 protein in non-small cell lung cancer （NSCLC） and explore the relationship between their expressions and the clinicopathological f...Objective： To investigate the expression offragile histidine triad （FHIT） and p53 protein in non-small cell lung cancer （NSCLC） and explore the relationship between their expressions and the clinicopathological features. Methods： FHIT protein and p53 protein were detected by immunohistochemistry in 76 cases of NSCLCs and matched normal lung tissues. Results： Fifty-one cases （67.1%） showed negative expression of FHIT （apparent reduction or loss） and thirty-seven cases （48.7%） showed p53 positive expression （overexpression）. The difference was significant （P=0.04）. However, there was no significant difference in FHIT expression between the p53-positive group and the p53-negative group （64.9% versus 69.2%, P=0.686）. The negative rate of FHIT protein expression was higher in squamous cell carcinoma than in adenocarcinoma, in moderately and poorly differentiated carcinoma than in well-differentiated carcinoma, and in cases with smoking history than in cases without smoking history （P〈0.05）. There was no relationship between FHIT expression and clinical stage or lymph node metastasis. The negative FHIT expression was not an independent predictor of overall survival （P=0.338）. Conclusion： The frequency of negative expression of FHIT protein is higher than that of positive expression of p53 in NSCLCs. The negative expression of FHIT is independent of the expression of p53. The change of expression of FHIT may play a role in the smoking related lung tumorigenesis while it may have no relationship with the progress of NSCLC or prognosis of the patients.展开更多
目的：体外评价表面修饰多聚赖氨酸的氧化铁磁性纳米颗粒作为野生型p53基因载体并转染肺癌细胞的可行性。方法：以碱沉淀法制成外包葡聚糖的氧化铁磁性生物纳米颗粒（dextran coated iron oxide nanoparticles，DCIONP），在其表面修饰...目的：体外评价表面修饰多聚赖氨酸的氧化铁磁性纳米颗粒作为野生型p53基因载体并转染肺癌细胞的可行性。方法：以碱沉淀法制成外包葡聚糖的氧化铁磁性生物纳米颗粒（dextran coated iron oxide nanoparticles，DCIONP），在其表面修饰多聚赖氨酸制成多聚赖氨酸-氧化铁纳米颗粒（dextran coated iron oxide nanoparticles modified with poly L-lysine．pll-DCIONP）。扫描电镜观察其形态特征，用激光粒度检测仪测定其粒度分布和Zeta表面电位；分光光度计及琼脂糖凝胶电泳分析pll-DCIONP与野生型P53基因的结合力及其复合物抵抗DNase-I和血清消化的能力；pll-DCIONP作为野生型p53基因载体转染人肺腺癌细胞A549／CDDP，RT—PCR以及Western印迹法分析细胞内p53基因的表达。结果：pll—DCIONP的直径在60～80nm之间，Zeta电位为＋19．6mV；pll-DCIONP无论在酸性、中性及碱性的条件下，均可与野生型P53基因结合，二者质量比为1：1时结合力最强；pll—DCIONP／wt—p53复合物能抵抗DNase—I和血清对野生型P53基因的消化作用；以pll—DCIONP作为野生型P53基因载体转染人肺腺癌细胞，随着时间延长，细胞内P53基因含量持续增高，而以脂质体作为转染载体时随着时间延长细胞内P53基因含量递减。结论：pll-DCIONP可作为野生型P53基因理想的转染载体，并能持续高效地将外源性P53基因转染入肺癌细胞中。展开更多
Objectives To evaluate the value of detecting p53 gene point mutations in sputum samples and its validity and reliability as a surveillance index in the early diagnosis of lung cancer in suspicious patients.Methods Sp...Objectives To evaluate the value of detecting p53 gene point mutations in sputum samples and its validity and reliability as a surveillance index in the early diagnosis of lung cancer in suspicious patients.Methods Sputum samples were collected from 54 cases identified as lung cancer and 114 cases identified as pulmonary benign disease. The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was performed for the detection of point mutations at exons 5-8 of the p53 gene, and sputum smears were also used for each sample. Conclusion Detection of p53 gene alterations in sputum samples by PCR-SSCP-silver stain can be used as a follow-up surveillance index for the early diagnosis of lung cancer in suspicious patients.展开更多
The expression of P53, p21WAF1 and proliferating cell nuclear antigen （PCNA） was detected in 114 samples of lung cancer patients （with 89 cases benign lung tissue as control） and the diagnostic value of these mark...The expression of P53, p21WAF1 and proliferating cell nuclear antigen （PCNA） was detected in 114 samples of lung cancer patients （with 89 cases benign lung tissue as control） and the diagnostic value of these markers was evaluated. The results show the following： （1） The positive expression rates of P53, p21WAF1 and PCNA in samples of lung cancer were 47.37%, 75.44% and 80.70%, respectively, which were significantly higher than that in the samples of benign lung diseases （ p 〈 0. 001 ）. The odds ratios were 39.15, 5.75, and 6.76, respectively. This indicates that the expression of P53, p21WAF1 and PCNA was helpful for the diagnosis of lung cancer. （2） For the diagnosis of lung cancer, the positive likelihood ratio of P53 was 21.08, which were significantly higher than that of p21WAF 1 （2.16）, PCNA （2.11） and of all the combined tests. This shows that P53 expression was the most valuable for diagnosis of lung cancer. （3） For the diagnosis of lung cancer, the negative likelihood ratio of P53/p21WAF 1/PCNA parallel test was 0.057 1, which was lower than that of other single and combined tests. This indicates that P53/p21WAF 1/PCNA parallel test has high diagnostic value for exclusion of lung cancer.展开更多
文摘Objective： To investigate the expression offragile histidine triad （FHIT） and p53 protein in non-small cell lung cancer （NSCLC） and explore the relationship between their expressions and the clinicopathological features. Methods： FHIT protein and p53 protein were detected by immunohistochemistry in 76 cases of NSCLCs and matched normal lung tissues. Results： Fifty-one cases （67.1%） showed negative expression of FHIT （apparent reduction or loss） and thirty-seven cases （48.7%） showed p53 positive expression （overexpression）. The difference was significant （P=0.04）. However, there was no significant difference in FHIT expression between the p53-positive group and the p53-negative group （64.9% versus 69.2%, P=0.686）. The negative rate of FHIT protein expression was higher in squamous cell carcinoma than in adenocarcinoma, in moderately and poorly differentiated carcinoma than in well-differentiated carcinoma, and in cases with smoking history than in cases without smoking history （P〈0.05）. There was no relationship between FHIT expression and clinical stage or lymph node metastasis. The negative FHIT expression was not an independent predictor of overall survival （P=0.338）. Conclusion： The frequency of negative expression of FHIT protein is higher than that of positive expression of p53 in NSCLCs. The negative expression of FHIT is independent of the expression of p53. The change of expression of FHIT may play a role in the smoking related lung tumorigenesis while it may have no relationship with the progress of NSCLC or prognosis of the patients.
文摘目的：体外评价表面修饰多聚赖氨酸的氧化铁磁性纳米颗粒作为野生型p53基因载体并转染肺癌细胞的可行性。方法：以碱沉淀法制成外包葡聚糖的氧化铁磁性生物纳米颗粒（dextran coated iron oxide nanoparticles，DCIONP），在其表面修饰多聚赖氨酸制成多聚赖氨酸-氧化铁纳米颗粒（dextran coated iron oxide nanoparticles modified with poly L-lysine．pll-DCIONP）。扫描电镜观察其形态特征，用激光粒度检测仪测定其粒度分布和Zeta表面电位；分光光度计及琼脂糖凝胶电泳分析pll-DCIONP与野生型P53基因的结合力及其复合物抵抗DNase-I和血清消化的能力；pll-DCIONP作为野生型p53基因载体转染人肺腺癌细胞A549／CDDP，RT—PCR以及Western印迹法分析细胞内p53基因的表达。结果：pll—DCIONP的直径在60～80nm之间，Zeta电位为＋19．6mV；pll-DCIONP无论在酸性、中性及碱性的条件下，均可与野生型P53基因结合，二者质量比为1：1时结合力最强；pll—DCIONP／wt—p53复合物能抵抗DNase—I和血清对野生型P53基因的消化作用；以pll—DCIONP作为野生型P53基因载体转染人肺腺癌细胞，随着时间延长，细胞内P53基因含量持续增高，而以脂质体作为转染载体时随着时间延长细胞内P53基因含量递减。结论：pll-DCIONP可作为野生型P53基因理想的转染载体，并能持续高效地将外源性P53基因转染入肺癌细胞中。
基金ThisresearchwassupportedbytheScienceandResearchFoundationof theMinistryofHealth (No :96 2 0 15 )
文摘Objectives To evaluate the value of detecting p53 gene point mutations in sputum samples and its validity and reliability as a surveillance index in the early diagnosis of lung cancer in suspicious patients.Methods Sputum samples were collected from 54 cases identified as lung cancer and 114 cases identified as pulmonary benign disease. The polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was performed for the detection of point mutations at exons 5-8 of the p53 gene, and sputum smears were also used for each sample. Conclusion Detection of p53 gene alterations in sputum samples by PCR-SSCP-silver stain can be used as a follow-up surveillance index for the early diagnosis of lung cancer in suspicious patients.
基金Supported by the Foundation of Health Department of Hubei Province ( 302140786)
文摘The expression of P53, p21WAF1 and proliferating cell nuclear antigen （PCNA） was detected in 114 samples of lung cancer patients （with 89 cases benign lung tissue as control） and the diagnostic value of these markers was evaluated. The results show the following： （1） The positive expression rates of P53, p21WAF1 and PCNA in samples of lung cancer were 47.37%, 75.44% and 80.70%, respectively, which were significantly higher than that in the samples of benign lung diseases （ p 〈 0. 001 ）. The odds ratios were 39.15, 5.75, and 6.76, respectively. This indicates that the expression of P53, p21WAF1 and PCNA was helpful for the diagnosis of lung cancer. （2） For the diagnosis of lung cancer, the positive likelihood ratio of P53 was 21.08, which were significantly higher than that of p21WAF 1 （2.16）, PCNA （2.11） and of all the combined tests. This shows that P53 expression was the most valuable for diagnosis of lung cancer. （3） For the diagnosis of lung cancer, the negative likelihood ratio of P53/p21WAF 1/PCNA parallel test was 0.057 1, which was lower than that of other single and combined tests. This indicates that P53/p21WAF 1/PCNA parallel test has high diagnostic value for exclusion of lung cancer.
