OBJECTIVE: To investigate the in vivo inhibitory effects of sodium houttuyfonate(SH) on symptom pattern of Qi-deficiency in rats induced by infection of bacterial biofilm on rat respiratory tract.METHODS: Symptom patt...OBJECTIVE: To investigate the in vivo inhibitory effects of sodium houttuyfonate(SH) on symptom pattern of Qi-deficiency in rats induced by infection of bacterial biofilm on rat respiratory tract.METHODS: Symptom pattern is a term used in Traditional Chinese Medicine(TCM) to define a cluster of symptoms in a medical condition. Based on the pattern, TCM therapies are administered. The symptom pattern used in this study was lung-Qi deficiency pattern identified in rats, which was induced by nasal intubation drip of Pseudomonas aeruginosa(P. aeruginosa)(two strains) to form bacterial biofilm on airway combined with stimulation of cold and fatigue. We measured the variations of the symptoms of the pattern, weight, spleen and thymus index, blood gas, lung bronchial tissue pathology and cytokine of rat in different treatments and control groups.RESULTS: The rats of SH-treatment groups had not showed typical symptoms comparing with model group in the early stage of infection. The weight,spleen and thymus index of the SH-treatment groups were significantly higher comparing with untreated model group. The SH-treatment groups also showed higher O2 partial pressure and lower CO2 partial pressure than model group. Furthermore, we found that the bronchopulmonary section of SH-treatment groups not showed typical pathogenic variation in model group. The comparison of cytokine concentration in different groups indicated that SH could prevent the over-production of cytokine to reduce the inflammation occurrence.CONCLUSION: In the early stage of airway infection by biofilm of P. aeruginosa, application of SH can prevent the occurrence of lung-Qi deficiency pattern.展开更多
Acquired immune deficiency syndrome （AIDS） is a chronic infectious disease,which the patients are infected with human immunodeficiency virus （HIV）.HIV damages the human＇s immune function and causes CD4 cell decli...Acquired immune deficiency syndrome （AIDS） is a chronic infectious disease,which the patients are infected with human immunodeficiency virus （HIV）.HIV damages the human＇s immune function and causes CD4 cell decline in the number and function.Immune reconstitution is an important treatment to AIDS.Bone marrow transplantation,adoptive immune cell therapy and cytokines infusion can all assist the immune reconstitution;highly active antiretroviral therapy （HAART） can effectively control the virus replication and benefit the immune reconstitution.HAART combined with immunotherapy is an important method of immune reconstitution in AIDS patients.Chinese medicine is playing a more and more important role in immune reconstitution.Immune reconstitution has always been effective in the whole treatment of AIDS.展开更多
Objective: To study the effect of Kanglaite (KLT) in treating pulmonary carcinoma (PC) patients with Lung-Qi Deficiency and immunologic inadequacy (LDII) after pneumonectomy. Methods: Thirtysix patients of PC-LDII wer...Objective: To study the effect of Kanglaite (KLT) in treating pulmonary carcinoma (PC) patients with Lung-Qi Deficiency and immunologic inadequacy (LDII) after pneumonectomy. Methods: Thirtysix patients of PC-LDII were treated with KLT and the lymphocyte subset was examined before and after treatment. Results: The markedly effective rate of treatment was 50. 0 % and the total effective rate 66. 7%. Immunological examination showed that the lymphocyte subset of patients tended to get normal and the difference between pre-treatment and post-treatment was significant. Conclusion: KLT could improve the symptom ofLung-Qi Deficiency and markedly strengthen the immune function of PC patients.展开更多
目的:观察四物汤对血虚证小鼠肝细胞凋亡及凋亡相关基因Bax、Bcl-2和Fas表达的影响。方法:30只C57BL/6J小鼠随机分为正常组、模型组和给药组,每组10只。给药组予四物汤灌胃20g·kg-1·d-1,正常组和模型组则予以等体积0.9%氯化...目的:观察四物汤对血虚证小鼠肝细胞凋亡及凋亡相关基因Bax、Bcl-2和Fas表达的影响。方法:30只C57BL/6J小鼠随机分为正常组、模型组和给药组,每组10只。给药组予四物汤灌胃20g·kg-1·d-1,正常组和模型组则予以等体积0.9%氯化钠溶液。以腹腔注射环磷酰胺建立血虚证模型,采用全自动血液分析仪检测外周血象,TUNNEL法检测肝细胞凋亡,荧光定量PCR法检测Bax、Bcl-2及Fas m RNA表达。结果:与模型组相比,给药组小鼠外周血红细胞数、白细胞数以及血红蛋白含量升高(P<0.05),血小板数无统计学差异,肝细胞凋亡百分率降低(P<0.05),促凋亡基因Bax、Fas m RNA表达水平下降(P<0.05),抗凋亡基因Bcl-2 m RNA表达无统计学差异。结论:四物汤可改善血虚证模型小鼠外周血象,下调促凋亡基因Bax和Fas表达,减少肝细胞凋亡。提示四物汤补益肝血作用机制可能与其减少肝细胞凋亡有关。展开更多
基金Supported by General Project of National Natural Science Foundation of China(No.81173629)Youth Project of National Natural Science Foundation of China(No.81503115)Key Project of Natural Science Foundation of Anhui University,China(No.KJ2015A139)
文摘OBJECTIVE: To investigate the in vivo inhibitory effects of sodium houttuyfonate(SH) on symptom pattern of Qi-deficiency in rats induced by infection of bacterial biofilm on rat respiratory tract.METHODS: Symptom pattern is a term used in Traditional Chinese Medicine(TCM) to define a cluster of symptoms in a medical condition. Based on the pattern, TCM therapies are administered. The symptom pattern used in this study was lung-Qi deficiency pattern identified in rats, which was induced by nasal intubation drip of Pseudomonas aeruginosa(P. aeruginosa)(two strains) to form bacterial biofilm on airway combined with stimulation of cold and fatigue. We measured the variations of the symptoms of the pattern, weight, spleen and thymus index, blood gas, lung bronchial tissue pathology and cytokine of rat in different treatments and control groups.RESULTS: The rats of SH-treatment groups had not showed typical symptoms comparing with model group in the early stage of infection. The weight,spleen and thymus index of the SH-treatment groups were significantly higher comparing with untreated model group. The SH-treatment groups also showed higher O2 partial pressure and lower CO2 partial pressure than model group. Furthermore, we found that the bronchopulmonary section of SH-treatment groups not showed typical pathogenic variation in model group. The comparison of cytokine concentration in different groups indicated that SH could prevent the over-production of cytokine to reduce the inflammation occurrence.CONCLUSION: In the early stage of airway infection by biofilm of P. aeruginosa, application of SH can prevent the occurrence of lung-Qi deficiency pattern.
基金Supported by the National Key Technologies Special-purpose Program (No.2008ZX10005-004)
文摘Acquired immune deficiency syndrome （AIDS） is a chronic infectious disease,which the patients are infected with human immunodeficiency virus （HIV）.HIV damages the human＇s immune function and causes CD4 cell decline in the number and function.Immune reconstitution is an important treatment to AIDS.Bone marrow transplantation,adoptive immune cell therapy and cytokines infusion can all assist the immune reconstitution;highly active antiretroviral therapy （HAART） can effectively control the virus replication and benefit the immune reconstitution.HAART combined with immunotherapy is an important method of immune reconstitution in AIDS patients.Chinese medicine is playing a more and more important role in immune reconstitution.Immune reconstitution has always been effective in the whole treatment of AIDS.
文摘Objective: To study the effect of Kanglaite (KLT) in treating pulmonary carcinoma (PC) patients with Lung-Qi Deficiency and immunologic inadequacy (LDII) after pneumonectomy. Methods: Thirtysix patients of PC-LDII were treated with KLT and the lymphocyte subset was examined before and after treatment. Results: The markedly effective rate of treatment was 50. 0 % and the total effective rate 66. 7%. Immunological examination showed that the lymphocyte subset of patients tended to get normal and the difference between pre-treatment and post-treatment was significant. Conclusion: KLT could improve the symptom ofLung-Qi Deficiency and markedly strengthen the immune function of PC patients.
文摘目的:观察四物汤对血虚证小鼠肝细胞凋亡及凋亡相关基因Bax、Bcl-2和Fas表达的影响。方法:30只C57BL/6J小鼠随机分为正常组、模型组和给药组,每组10只。给药组予四物汤灌胃20g·kg-1·d-1,正常组和模型组则予以等体积0.9%氯化钠溶液。以腹腔注射环磷酰胺建立血虚证模型,采用全自动血液分析仪检测外周血象,TUNNEL法检测肝细胞凋亡,荧光定量PCR法检测Bax、Bcl-2及Fas m RNA表达。结果:与模型组相比,给药组小鼠外周血红细胞数、白细胞数以及血红蛋白含量升高(P<0.05),血小板数无统计学差异,肝细胞凋亡百分率降低(P<0.05),促凋亡基因Bax、Fas m RNA表达水平下降(P<0.05),抗凋亡基因Bcl-2 m RNA表达无统计学差异。结论:四物汤可改善血虚证模型小鼠外周血象,下调促凋亡基因Bax和Fas表达,减少肝细胞凋亡。提示四物汤补益肝血作用机制可能与其减少肝细胞凋亡有关。
