The overpass at Dongbianmen was opened to the public in 1988. Its design and construction were difficult because the ancient buildings nearby needed to be protected. The design was complicated because a highway, a rai...The overpass at Dongbianmen was opened to the public in 1988. Its design and construction were difficult because the ancient buildings nearby needed to be protected. The design was complicated because a highway, a railway and a river intersect here.展开更多
目的建立RP-HPLC双波长法同时测定活络效灵丹中3种丹参酮和6种乳香酸的含量。方法采用RP-HPLC法,色谱柱为Waters x BridgeTmC18柱(150 mm×4.6 mm,3.5μm),流动相为体积分数为0.01%的磷酸水溶液-乙腈,梯度洗脱,流速为1.0 m L·...目的建立RP-HPLC双波长法同时测定活络效灵丹中3种丹参酮和6种乳香酸的含量。方法采用RP-HPLC法,色谱柱为Waters x BridgeTmC18柱(150 mm×4.6 mm,3.5μm),流动相为体积分数为0.01%的磷酸水溶液-乙腈,梯度洗脱,流速为1.0 m L·min^-1,检测波长为210 nm和254 nm,柱温为30℃。结果隐丹参酮、丹参酮I、丹参酮IIA、11-羰基-β-乳香酸(KBA)、11-羰基-β-乙酰乳香酸(AKBA)、α-乳香酸(α-BA)、β-乳香酸(β-BA)、乙酰基-α-乳香酸(A-α-BA)和乙酰基-β-乳香酸(A-β-BA)的质量浓度分别在0.252 0~8.064、0.157 2~5.030、0.937 5~30.00、3.156~101.0、25.10~803.2、6.250~200.0、6.813~218.0、13.50~432.0、12.50~400.0 mg·L-1内与峰面积呈良好的线性关系(r≥0.999 3,n=6),提取回收率均在97.2%~101.2%内。结论该方法可做为活络效灵丹中丹参酮类和乳香酸类化学成分的含量测定方法,为其质量控制提供参考依据。展开更多
文摘The overpass at Dongbianmen was opened to the public in 1988. Its design and construction were difficult because the ancient buildings nearby needed to be protected. The design was complicated because a highway, a railway and a river intersect here.