To explore genetic diversity and estimate the genetic differences among populations of Lutraria maxima in the coastal waters off south to southeast China,the morphology of the species of five different geographical po...To explore genetic diversity and estimate the genetic differences among populations of Lutraria maxima in the coastal waters off south to southeast China,the morphology of the species of five different geographical populations(Beihai,Weizhou Island,Zhanjiang,Xiamen,and Fuzhou)in Guangxi,Guangdong,and Fujian provinces was studied statistically in combination with the microsatellite markers.As revealed by morphological principal component analysis(PCA),the cumulative contribution rate of the first three principal components was 72.596%.The discrimination accuracy ranged from 47.5%to 80.0%,and the scatter plots of principal component and discriminant analysis were consistent in overall,showing that the Xiamen and Fuzhou populations were overlapped obviously.For microsatellite markers,10 pairs of polymorphic primers were obtained by high-throughput transcriptome sequencing,and used for genetic diversity analysis.It was showed that the average number of alleles and eff ective alleles observed in each population ranged from 8.100 to 10.900,and from 3.497 to 4.228,respectively.The average observed heterozygosity(H_(o))and expected heterozygosity(H_(e))in the five populations ranged from 0.541 to 0.615,and from 0.642 to 0.733,respectively.The genetic distance(DA)ranged from 0.078 to 0.523,and the population genetic differentiation index(F_(ST))ranged from 0.027 to 0.139.The unweighted pair-population method with arithmetic means(UPGMA)and structure analysis showed that the five populations could be divided into two main clusters,the Beibu Gulf group(Beihai and Weizhou Island)and the Southeast China Sea group(Zhanjiang,Xiamen,and Fuzhou),suggesting that L.maxima has been separated geographically by the barrier of the Leizhou Peninsula into two groups in evolution,which provided us with a scientific clue to better protect the bioresource and establish an appropriate fishery management stocks for L.maxima populations in south China.展开更多
[Objectives]This study was conducted to explore the relationship between morphological traits and body mass trait of Lutraria sieboldii.[Methods]110 were randomly selected from 120 2^(nd)-instar L.sieboldii collected ...[Objectives]This study was conducted to explore the relationship between morphological traits and body mass trait of Lutraria sieboldii.[Methods]110 were randomly selected from 120 2^(nd)-instar L.sieboldii collected from the Tieshangang area of Beihai,Guangxi,and 132 were randomly selected from 150 shellfish at the instar of 0.6.Their morphological traits were measured:shell length(SL),shell height(SH),shell width(SW),anterior length(AL),posterior length(PL),nose length(NL)in closed shell state,and maximum open shell width(OS)between two shells in closed shell state,and the body mass trait BM was also measured.Statistical methods such as path analysis and multiple regression were used for data analysis,and the effects of these seven morphological traits on the body mass trait were studied,respectively.The correlation between the tested seven quantitative traits and one body mass trait was all positive,all reaching an extremely significant level(P<0.01).[Results]The body mass trait of the shellfish at the instar of 2 had the highest correlation coefficient with shell length(0.922),that is,shell length had the greatest direct impact on the body mass trait;the path coefficient was 0.700;and the final multiple regression equation established was BM=-124.882+1.189 SL+1.551 SH+1.035 SW+0.119 NL,and the total determination coefficient(R^(2))on body mass was 0.849.The body mass trait of the shellfish at the instar of 0.6 had the highest correlation coefficient with shell length(0.859),that is,shell length had the greatest direct impact on the body mass trait;the path coefficient was 0.494;and the final multiple regression equation established was BM=-1.917+0.111 SL+0.021 NL+0.078 SW+0.032 OS,and the total determination coefficient(R~2)on body mass was 0.828.The multivariate regression variance analysis showed that the regression between the morphological traits and body mass trait of the L.sieboldii at the instars of 2 and 0.6 reached an extremely significant level(P<0.01).[Conclusions]This study provides a scientific basis for the selection of broodstock in the selection and breeding of L.sieboldii.展开更多
基金Supported by the Science and Technology Major Project of Guangxi Province(No.AA17204088)。
文摘To explore genetic diversity and estimate the genetic differences among populations of Lutraria maxima in the coastal waters off south to southeast China,the morphology of the species of five different geographical populations(Beihai,Weizhou Island,Zhanjiang,Xiamen,and Fuzhou)in Guangxi,Guangdong,and Fujian provinces was studied statistically in combination with the microsatellite markers.As revealed by morphological principal component analysis(PCA),the cumulative contribution rate of the first three principal components was 72.596%.The discrimination accuracy ranged from 47.5%to 80.0%,and the scatter plots of principal component and discriminant analysis were consistent in overall,showing that the Xiamen and Fuzhou populations were overlapped obviously.For microsatellite markers,10 pairs of polymorphic primers were obtained by high-throughput transcriptome sequencing,and used for genetic diversity analysis.It was showed that the average number of alleles and eff ective alleles observed in each population ranged from 8.100 to 10.900,and from 3.497 to 4.228,respectively.The average observed heterozygosity(H_(o))and expected heterozygosity(H_(e))in the five populations ranged from 0.541 to 0.615,and from 0.642 to 0.733,respectively.The genetic distance(DA)ranged from 0.078 to 0.523,and the population genetic differentiation index(F_(ST))ranged from 0.027 to 0.139.The unweighted pair-population method with arithmetic means(UPGMA)and structure analysis showed that the five populations could be divided into two main clusters,the Beibu Gulf group(Beihai and Weizhou Island)and the Southeast China Sea group(Zhanjiang,Xiamen,and Fuzhou),suggesting that L.maxima has been separated geographically by the barrier of the Leizhou Peninsula into two groups in evolution,which provided us with a scientific clue to better protect the bioresource and establish an appropriate fishery management stocks for L.maxima populations in south China.
基金Supported by Guangxi Key R&D Program(2018AB52002)National Key R&D Program of China(2018YFD0901406)+3 种基金Natural Science Foundation of Guangxi(2018GXNSFAA138197,2021GXNSFAA075008)General Project of National Natural Science Foundation of China(31873042)2021 Key Cultivation Project of Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation(2021ZB02)The Basic Ability Improvement Project for Young and Middle-aged Teachers in Guangxi Universities(2018KY0612)。
文摘[Objectives]This study was conducted to explore the relationship between morphological traits and body mass trait of Lutraria sieboldii.[Methods]110 were randomly selected from 120 2^(nd)-instar L.sieboldii collected from the Tieshangang area of Beihai,Guangxi,and 132 were randomly selected from 150 shellfish at the instar of 0.6.Their morphological traits were measured:shell length(SL),shell height(SH),shell width(SW),anterior length(AL),posterior length(PL),nose length(NL)in closed shell state,and maximum open shell width(OS)between two shells in closed shell state,and the body mass trait BM was also measured.Statistical methods such as path analysis and multiple regression were used for data analysis,and the effects of these seven morphological traits on the body mass trait were studied,respectively.The correlation between the tested seven quantitative traits and one body mass trait was all positive,all reaching an extremely significant level(P<0.01).[Results]The body mass trait of the shellfish at the instar of 2 had the highest correlation coefficient with shell length(0.922),that is,shell length had the greatest direct impact on the body mass trait;the path coefficient was 0.700;and the final multiple regression equation established was BM=-124.882+1.189 SL+1.551 SH+1.035 SW+0.119 NL,and the total determination coefficient(R^(2))on body mass was 0.849.The body mass trait of the shellfish at the instar of 0.6 had the highest correlation coefficient with shell length(0.859),that is,shell length had the greatest direct impact on the body mass trait;the path coefficient was 0.494;and the final multiple regression equation established was BM=-1.917+0.111 SL+0.021 NL+0.078 SW+0.032 OS,and the total determination coefficient(R~2)on body mass was 0.828.The multivariate regression variance analysis showed that the regression between the morphological traits and body mass trait of the L.sieboldii at the instars of 2 and 0.6 reached an extremely significant level(P<0.01).[Conclusions]This study provides a scientific basis for the selection of broodstock in the selection and breeding of L.sieboldii.
