Al-for-one tourism plays an increasingly important role in the development of national tourism,and rural landscape is endowed with multiple functions in all-for-one tourism pattern.After Yuan'an County was listed ...Al-for-one tourism plays an increasingly important role in the development of national tourism,and rural landscape is endowed with multiple functions in all-for-one tourism pattern.After Yuan'an County was listed as the second batch of national all-in-one tourism demonstration zones,the establishment of al-in-one tourism puts forward higher requirements for the planning and promotion of rural landscape in Yuan'an County.By analyzing the status quo of rural landscape construction through landscape cultural characteristics,landscape differentiation and landscape management mechanism,combined with the current style of Luyuan Village in Yuan'an County,the paper discusses the promotion strategy and implementation approach of rural landscape in the perspective of all-region tourism from natural ecological landscape,settlement life landscape and economic production landscape,in order to bring new thinking for the improvement of rural landscape in China.展开更多
目的探讨绿原胶囊浸出液对成人外周血来源的树突状细胞(Dendritic cells,DCs)增殖及成熟的影响。方法采集健康男性(4名)外周血各100mL,采用密度梯度离心法获取外周血单个核细胞,贴壁法获取弱贴壁的DCs前体细胞,用添加重组人粒细胞-巨噬...目的探讨绿原胶囊浸出液对成人外周血来源的树突状细胞(Dendritic cells,DCs)增殖及成熟的影响。方法采集健康男性(4名)外周血各100mL,采用密度梯度离心法获取外周血单个核细胞,贴壁法获取弱贴壁的DCs前体细胞,用添加重组人粒细胞-巨噬细胞集落刺激因子(recombinant human GM-CSF,rhGM-CSF)和重组人白细胞介素4(recombinant human IL-4,rhIL-4)的RPML-1640的DCs培养基培养DCs前体细胞7天,可获得未成熟的DCs。以在DCs培养体系中加入1%浓度的绿原胶囊浸出液共培养未成熟DCs2天作为实验组,以20ng/mL重组人肿瘤坏死因子(Recombinant human TNF-α,rh TNF-α)共培养未成熟DCs2天作为对照组。倒置相差显微镜观察细胞形态,细胞计数法检测成熟DCs增殖能力,流式细胞仪检测成熟DCs表达的特异性标记物CD83。结果与对照组相比,实验组培养2天后,DCs的增殖能力提高,DCs表面呈现出比对照组更多和更长的树突状突起,实验组CD83表达[(84.1±0.3)%]明显高于对照组[(58.7±0.2)%],两组比较差异有统计学意义(P<0.05)。结论 1%浓度的绿原胶囊浸出液可促进外周血来源的DCs成熟和增殖,且作用较20ng/mL的rh TNF-α更强。展开更多
文摘Al-for-one tourism plays an increasingly important role in the development of national tourism,and rural landscape is endowed with multiple functions in all-for-one tourism pattern.After Yuan'an County was listed as the second batch of national all-in-one tourism demonstration zones,the establishment of al-in-one tourism puts forward higher requirements for the planning and promotion of rural landscape in Yuan'an County.By analyzing the status quo of rural landscape construction through landscape cultural characteristics,landscape differentiation and landscape management mechanism,combined with the current style of Luyuan Village in Yuan'an County,the paper discusses the promotion strategy and implementation approach of rural landscape in the perspective of all-region tourism from natural ecological landscape,settlement life landscape and economic production landscape,in order to bring new thinking for the improvement of rural landscape in China.
文摘目的探讨绿原胶囊浸出液对成人外周血来源的树突状细胞(Dendritic cells,DCs)增殖及成熟的影响。方法采集健康男性(4名)外周血各100mL,采用密度梯度离心法获取外周血单个核细胞,贴壁法获取弱贴壁的DCs前体细胞,用添加重组人粒细胞-巨噬细胞集落刺激因子(recombinant human GM-CSF,rhGM-CSF)和重组人白细胞介素4(recombinant human IL-4,rhIL-4)的RPML-1640的DCs培养基培养DCs前体细胞7天,可获得未成熟的DCs。以在DCs培养体系中加入1%浓度的绿原胶囊浸出液共培养未成熟DCs2天作为实验组,以20ng/mL重组人肿瘤坏死因子(Recombinant human TNF-α,rh TNF-α)共培养未成熟DCs2天作为对照组。倒置相差显微镜观察细胞形态,细胞计数法检测成熟DCs增殖能力,流式细胞仪检测成熟DCs表达的特异性标记物CD83。结果与对照组相比,实验组培养2天后,DCs的增殖能力提高,DCs表面呈现出比对照组更多和更长的树突状突起,实验组CD83表达[(84.1±0.3)%]明显高于对照组[(58.7±0.2)%],两组比较差异有统计学意义(P<0.05)。结论 1%浓度的绿原胶囊浸出液可促进外周血来源的DCs成熟和增殖,且作用较20ng/mL的rh TNF-α更强。
