To accelerate the breeding process, male sterile line is used to leave out the troublesome procedures of the artificial emasculation in tomato breeding. However, the fertility of the pollen thermo-sensitivity male ste...To accelerate the breeding process, male sterile line is used to leave out the troublesome procedures of the artificial emasculation in tomato breeding. However, the fertility of the pollen thermo-sensitivity male sterile line (PTMSL) and the stigma exsertion male sterile line (SEMSL) are affected easily by the environments when used alone. The trial materials were Da107 and the control was First. This study was conducted to create a new male sterile line of tomato characterized by pollen thermo-sensitivity and long style by genic recombination through the hybridizing of the PTMSL and SEMSL. Research on the statistics of the pollen germination rate, the contamination rate in F1 and the flower organics indicated that Da107 was an ideal TS and SE male sterile line with the sterility of 95%, as well as it also could be used as fertile line at low temperature. Meanwhile, the results showed that hybrid-seed contamination risk with selfed seeds from residual fertility in Da107 was low.展开更多
A study was conducted with 36 tomato germplasm [PGRC (19), The Netherlands (7), Japan (2), and Bangladesh (9)] with 3 check varieties in the research field of the Olericulture Division of Horticulture Research Centre ...A study was conducted with 36 tomato germplasm [PGRC (19), The Netherlands (7), Japan (2), and Bangladesh (9)] with 3 check varieties in the research field of the Olericulture Division of Horticulture Research Centre (HRC), Bangladesh Agricultural Research Institute (BARI), Gazipur during the winter season of 2022-23 to identify promising tomato breeding germplasm. All the germplasm showed differences in most of the parameters studied. Considerable variation was observed for fruit yield per hectare varied from 39 to 144 tons, while the highest fruit yield per hectare was harvested from the germplasm viz., BD 7759B, BD 7757, BD 7751, BD 7301, QM, BD 7759A, BD 7762, QF, BD 7753, BD 8886, BD 10351, NL-1A, SLA-19. The number of locules, total soluble solids (TSS) and plant height at last harvest varied from 2 to 6.6, 2.6 to 5.9%, and 66 to 154cm, respectively. The TYLCV infection (%) and leaf-sucking pest infestation (%) were observed with a 1 to 20% range in the field condition in both cases. So, we can select that germplasm has zero per cent infestation. Considering the plant growth habit, earliness, different yield contributing horticultural traits, virus and pest infestation thirteen germplasm viz., BD 7759B, BD 7757, BD 7751, BD 7301, QM, BD 7759A, BD 7762, QF, BD 7753, BD 8886, BD 10351, NL-1A, SLA-19 were selected as breeding materials for further tomato improvement program.展开更多
Ulcerative colitis(UC)is a recurrent inflammatory bowel disease that imposes a severe burden on families and society.In recent years,exploiting the potential of marine bioactive peptides for the treatment of diseases ...Ulcerative colitis(UC)is a recurrent inflammatory bowel disease that imposes a severe burden on families and society.In recent years,exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research interest.This study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV(Pro-Lys-Lys-Val-Val)of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum metabolome.Specifically,the polypeptide composition of R.esculentum cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF/TOF-MS).Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α(TNF-α)than the original ligand.Subsequent animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC;affect serum metabolites through the arachidonic acid,glycerophospholipid and linoleic acid metabolism pathways;and further alleviate UC symptoms.This study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.展开更多
The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total ...The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total phenolic content （TPC）, as well as 1,1 diphenyl-2-picryl hydrazyl （DPPH） free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average ECs0 84.54 μg mL^-1 and IC50 11.54 μg mL^-1 respectively, FTG brans extract had the highest average TPC （24.87 mg GAE g^-1 DW）, and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient （rs） between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively （P〈0.05）. However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.展开更多
Brassinosteroids (BRs) and polyamines (PAs) are widely used to overcome abiotic stresses including salinity stress (NaCl) in plants. In the present investigation, we evaluated the co-application efficacy of 24-epibras...Brassinosteroids (BRs) and polyamines (PAs) are widely used to overcome abiotic stresses including salinity stress (NaCl) in plants. In the present investigation, we evaluated the co-application efficacy of 24-epibrassinolide (EBR, a highly active BR) and putrescine (Put, a PA) on the NaCl stress (75 mM and 150 mM) tolerance of Lycopersicon esculentum L. cv. kuber geeta plants. A small rise in protein content was recorded under salinity stress in comparison with untreated control. The NaCl stress was found to significantly enhance the activities of guaiacol peroxidase (GPOX) and superoxide dismutase (SOD);while decline in catalase (CAT) activity was recorded when compared with the untreated control. Salinity stress both at 75 mM and 150 mM was able to cause significant membrane damage as evidenced by an increase in the level of malondialdehyde (MDA) content over untreated control. The EBR and Put co-applications were able to improve protein content in NaCl stressed plants over only NaCl stressed plants. The co-applications of EBR and Put were able to significantly enhance the activities of CAT, SOD and GPOX in L. esculentum under salinity stress (75 mM and 150 mM) when compared with NaCl stressed plants alone. Major decline in the MDA level recorded for EBR and Put co-applications under NaCl stress revealed reduced membrane damages when compared with NaCl stressed plants alone. Our findings provide evidence that EBR and Put co-applications are effective in amelioration of NaCl stress in L. esculentum. Thus co-application potential of EBR and Put may acts an eco-friendly approach towards NaCl stress mitigation in economically important crops.展开更多
A numerical model for jellyfish Rhopilema esculentum stock enhancement is developed for the first time. The model is based on an operational ocean circulation-surface wave coupled forecasting system for the seas off C...A numerical model for jellyfish Rhopilema esculentum stock enhancement is developed for the first time. The model is based on an operational ocean circulation-surface wave coupled forecasting system for the seas off China and adjacent areas and uses a Lagrangian particle-tracking scheme to track the trajectories of released jellyfish. The Jellyfish are modeled as particles with diel vertical migration and are passively drifted by the current and dispersion due to the sub-grid processes. A comparison between the simulation and survey results demonstrate that the model can capture the primary distribution patterns of the released jellyfish. The model results show that the ocean current and indirect wind impact are the main drivers controlling the jellyfish transport. A connectivity matrix between the release sites and fishing grounds indicates the top of the bay is better than the eastern and western coasts for jellyfish fishing. The matrix also shows that only 45% and 27% of the jellyfish released from Wafangdian(WFD) can enter the fishing ground in 2008 and 2010; thus, the site near WFD is not an advisable location for jellyfish release. A Lagrangian probability density function based on a nine-year tracing experiment validates the results and further provides a "climatology" distribution of the released jellyfish.Several experiments are conducted to examine the sensitivity of the model to random walk schemes and to release conditions. The model requires a random walk but is insensitive to the random walk scheme. The experiments with different habitat depths show that if the jellyfish are fixed on the bottom of the water, most of them will be transported to the center, or even out of the bay, by the bottom circulation.展开更多
Amplified fragment length polymorphisms(AFLP) markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye(Scyphozoa,Rhizostomatidae).One hundred and seventy-nine...Amplified fragment length polymorphisms(AFLP) markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye(Scyphozoa,Rhizostomatidae).One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations were genotyped with five primer combinations.The polymorphic ratio,Shannon's diversity index and average heterozygosity were 70.3%,0.346 and 0.228 for the white hatchery population,74.3%,0.313,and 0.201 for the red hatchery population,79.3%,0.349,and 0.224 for the Jiangsu wild population,and 74.9%,0.328 and 0.210 for the Penglai wild population,respectively.Thus,all populations had a relatively high level of genetic diversity.A specific band was identified that could separate the white from the red hatchery population.There was 84.85% genetic differentiation within populations.Individual cluster analysis using unweighted pair-group method with arithmetic mean(UPGMA) suggested that hatchery populations and wild populations could be divided.For the hatchery populations,the white and red populations clustered separately;however,for the wild populations,Penglai and Jiangsu populations clustered together.The genetic diversity at the clone level was also determined.Our data suggest that there are relatively high genetic diversities within populations but low genetic differentiation between populations,which may be related to the long-term use of germplasm resources from Jiangsu Province for artificial seeding and releasing.These findings will benefit the artificial seeding and conservation of the germplasm resources.展开更多
Tomato (Lycopersicon esculentum) is one of the important vegetables in supplying vitamins, minerals and fiber to human diets worldwide. Its successful production in the tropics is, however, constrained by environmenta...Tomato (Lycopersicon esculentum) is one of the important vegetables in supplying vitamins, minerals and fiber to human diets worldwide. Its successful production in the tropics is, however, constrained by environmental variations especially under open field conditions. Two trials were conducted at the Horticulture Research and Teaching Field, Egerton University, Kenya to evaluate the effects of agricultural nets (agronets) herein called eco-friendly nets (EFNs) and floating row covers (FRCs) on microclimate modification, yield, and quality of tomato. A randomized complete block design with five replications was used. Tomato plants were grown under fine mesh EFN (0.4-mm pore diameter) cover, large mesh EFN (0.9-mm pore diameter) cover or FRC. The EFN and FRC were maintained either permanently closed or opened thrice a week from 9 am to 3 pm. Two open control treatments were used: unsprayed (untreated control) or sprayed with chemicals (treated control). The use of EFN or FRC modified the microclimate with higher temperatures, lower diurnal temperature ranges, and higher volumetric water content recorded compared with the controls. On the other hand, light quantity and photosynthetic active radiation were reduced by the use of EFN and FRC compared with the controls. The use of FRC and EFN resulted in more fruit and higher percent in marketable yield compared with open field production. Fruit quality at harvest was also significantly improved by the use of EFN and FRC. Fruits with higher total soluble solids (TSS), lower titratable acidity (TA), and higher sugar acid ratio were obtained in EFN and FRC treatments compared with the controls. Fruits harvested from EFN and FRC were also firmer compared with control fruits. These findings demonstrate the potential of EFN and FRC in modifying microclimate conditions and improving yields and quality of tomato under tropical field conditions.展开更多
The effects of Nd^3＋ on the quality and quantity of volatile organic compounds （VOCs） in the leaves of Lycopersicon esculentum were studied. The results demonstrate that Nd^3＋ can increase the total amount of VOC ...The effects of Nd^3＋ on the quality and quantity of volatile organic compounds （VOCs） in the leaves of Lycopersicon esculentum were studied. The results demonstrate that Nd^3＋ can increase the total amount of VOC by 75% after treatment for 120 h, as compared with the control. Phyto-oxylipins, terpenoids and aromatic compounds were increased by 73%, 38% and 21%, respectively. （E）-2-hexenal, the most abundant constituent is increased by 74%, β- phellandrene and α-caryophyllene in terpenoids,展开更多
文摘To accelerate the breeding process, male sterile line is used to leave out the troublesome procedures of the artificial emasculation in tomato breeding. However, the fertility of the pollen thermo-sensitivity male sterile line (PTMSL) and the stigma exsertion male sterile line (SEMSL) are affected easily by the environments when used alone. The trial materials were Da107 and the control was First. This study was conducted to create a new male sterile line of tomato characterized by pollen thermo-sensitivity and long style by genic recombination through the hybridizing of the PTMSL and SEMSL. Research on the statistics of the pollen germination rate, the contamination rate in F1 and the flower organics indicated that Da107 was an ideal TS and SE male sterile line with the sterility of 95%, as well as it also could be used as fertile line at low temperature. Meanwhile, the results showed that hybrid-seed contamination risk with selfed seeds from residual fertility in Da107 was low.
文摘A study was conducted with 36 tomato germplasm [PGRC (19), The Netherlands (7), Japan (2), and Bangladesh (9)] with 3 check varieties in the research field of the Olericulture Division of Horticulture Research Centre (HRC), Bangladesh Agricultural Research Institute (BARI), Gazipur during the winter season of 2022-23 to identify promising tomato breeding germplasm. All the germplasm showed differences in most of the parameters studied. Considerable variation was observed for fruit yield per hectare varied from 39 to 144 tons, while the highest fruit yield per hectare was harvested from the germplasm viz., BD 7759B, BD 7757, BD 7751, BD 7301, QM, BD 7759A, BD 7762, QF, BD 7753, BD 8886, BD 10351, NL-1A, SLA-19. The number of locules, total soluble solids (TSS) and plant height at last harvest varied from 2 to 6.6, 2.6 to 5.9%, and 66 to 154cm, respectively. The TYLCV infection (%) and leaf-sucking pest infestation (%) were observed with a 1 to 20% range in the field condition in both cases. So, we can select that germplasm has zero per cent infestation. Considering the plant growth habit, earliness, different yield contributing horticultural traits, virus and pest infestation thirteen germplasm viz., BD 7759B, BD 7757, BD 7751, BD 7301, QM, BD 7759A, BD 7762, QF, BD 7753, BD 8886, BD 10351, NL-1A, SLA-19 were selected as breeding materials for further tomato improvement program.
基金sponsored by the National Key R&D Program of China (2018YFD0901102)the Natural Science Foundation of Zhejiang Province (LQ22D060002)+2 种基金the Fund of State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products (ZS20190105)the Fundamental Research Funds for the Provincial Universities of Zhejiang (SJLY2021015)the K.C.Wong Magna Fund of Ningbo University。
文摘Ulcerative colitis(UC)is a recurrent inflammatory bowel disease that imposes a severe burden on families and society.In recent years,exploiting the potential of marine bioactive peptides for the treatment of diseases has become a topic of intense research interest.This study revealed the mechanism underlying the protective effect of the dominant polypeptide PKKVV(Pro-Lys-Lys-Val-Val)of Rhopilema esculentum cnidoblasts against DSS-induced UC through a combined analysis of the metagenome and serum metabolome.Specifically,the polypeptide composition of R.esculentum cnidoblasts was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF/TOF-MS).Molecular docking showed that the dominant peptide PKKVV could bind better with tumor necrosis factor-α(TNF-α)than the original ligand.Subsequent animal experiments suggested that PKKVV could modulate disorganized gut microorganisms in mice with UC;affect serum metabolites through the arachidonic acid,glycerophospholipid and linoleic acid metabolism pathways;and further alleviate UC symptoms.This study provides a reference for the comprehensive development of marine bioactive substances and nonpharmaceutical treatments for UC.
基金supported by the Fundamental Research Funds for the Central Universities,China (XDJK2012B014)
文摘The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total phenolic content （TPC）, as well as 1,1 diphenyl-2-picryl hydrazyl （DPPH） free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average ECs0 84.54 μg mL^-1 and IC50 11.54 μg mL^-1 respectively, FTG brans extract had the highest average TPC （24.87 mg GAE g^-1 DW）, and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient （rs） between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively （P〈0.05）. However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.
文摘Brassinosteroids (BRs) and polyamines (PAs) are widely used to overcome abiotic stresses including salinity stress (NaCl) in plants. In the present investigation, we evaluated the co-application efficacy of 24-epibrassinolide (EBR, a highly active BR) and putrescine (Put, a PA) on the NaCl stress (75 mM and 150 mM) tolerance of Lycopersicon esculentum L. cv. kuber geeta plants. A small rise in protein content was recorded under salinity stress in comparison with untreated control. The NaCl stress was found to significantly enhance the activities of guaiacol peroxidase (GPOX) and superoxide dismutase (SOD);while decline in catalase (CAT) activity was recorded when compared with the untreated control. Salinity stress both at 75 mM and 150 mM was able to cause significant membrane damage as evidenced by an increase in the level of malondialdehyde (MDA) content over untreated control. The EBR and Put co-applications were able to improve protein content in NaCl stressed plants over only NaCl stressed plants. The co-applications of EBR and Put were able to significantly enhance the activities of CAT, SOD and GPOX in L. esculentum under salinity stress (75 mM and 150 mM) when compared with NaCl stressed plants alone. Major decline in the MDA level recorded for EBR and Put co-applications under NaCl stress revealed reduced membrane damages when compared with NaCl stressed plants alone. Our findings provide evidence that EBR and Put co-applications are effective in amelioration of NaCl stress in L. esculentum. Thus co-application potential of EBR and Put may acts an eco-friendly approach towards NaCl stress mitigation in economically important crops.
基金The National Natural Science Foundation of China under contract No.41506044the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology of China under contract No.2016ASKJ02+2 种基金the National Basic Research Program(973 Program)of China under contract No.2015CB453303the National Natural Science Foundation of China-Shandong Joint Fund for Marine Science Research Centers under contract No.U1606405the International Cooperation Project of Indo-Pacific Ocean Environment Variation and Air-Sea Interaction under contract No.GASI-03-IPOVAI-05
文摘A numerical model for jellyfish Rhopilema esculentum stock enhancement is developed for the first time. The model is based on an operational ocean circulation-surface wave coupled forecasting system for the seas off China and adjacent areas and uses a Lagrangian particle-tracking scheme to track the trajectories of released jellyfish. The Jellyfish are modeled as particles with diel vertical migration and are passively drifted by the current and dispersion due to the sub-grid processes. A comparison between the simulation and survey results demonstrate that the model can capture the primary distribution patterns of the released jellyfish. The model results show that the ocean current and indirect wind impact are the main drivers controlling the jellyfish transport. A connectivity matrix between the release sites and fishing grounds indicates the top of the bay is better than the eastern and western coasts for jellyfish fishing. The matrix also shows that only 45% and 27% of the jellyfish released from Wafangdian(WFD) can enter the fishing ground in 2008 and 2010; thus, the site near WFD is not an advisable location for jellyfish release. A Lagrangian probability density function based on a nine-year tracing experiment validates the results and further provides a "climatology" distribution of the released jellyfish.Several experiments are conducted to examine the sensitivity of the model to random walk schemes and to release conditions. The model requires a random walk but is insensitive to the random walk scheme. The experiments with different habitat depths show that if the jellyfish are fixed on the bottom of the water, most of them will be transported to the center, or even out of the bay, by the bottom circulation.
基金Supported by the Taishan Scholarship of Aquatic Animal Nutrition and Feed and the National Marine Public Welfare Research Project(Nos.201205025,201305001)
文摘Amplified fragment length polymorphisms(AFLP) markers were developed to assess the genetic variation of populations and clones of Rhopilema esculentum Kishinouye(Scyphozoa,Rhizostomatidae).One hundred and seventy-nine loci from 56 individuals of two hatchery populations and two wild populations were genotyped with five primer combinations.The polymorphic ratio,Shannon's diversity index and average heterozygosity were 70.3%,0.346 and 0.228 for the white hatchery population,74.3%,0.313,and 0.201 for the red hatchery population,79.3%,0.349,and 0.224 for the Jiangsu wild population,and 74.9%,0.328 and 0.210 for the Penglai wild population,respectively.Thus,all populations had a relatively high level of genetic diversity.A specific band was identified that could separate the white from the red hatchery population.There was 84.85% genetic differentiation within populations.Individual cluster analysis using unweighted pair-group method with arithmetic mean(UPGMA) suggested that hatchery populations and wild populations could be divided.For the hatchery populations,the white and red populations clustered separately;however,for the wild populations,Penglai and Jiangsu populations clustered together.The genetic diversity at the clone level was also determined.Our data suggest that there are relatively high genetic diversities within populations but low genetic differentiation between populations,which may be related to the long-term use of germplasm resources from Jiangsu Province for artificial seeding and releasing.These findings will benefit the artificial seeding and conservation of the germplasm resources.
文摘Tomato (Lycopersicon esculentum) is one of the important vegetables in supplying vitamins, minerals and fiber to human diets worldwide. Its successful production in the tropics is, however, constrained by environmental variations especially under open field conditions. Two trials were conducted at the Horticulture Research and Teaching Field, Egerton University, Kenya to evaluate the effects of agricultural nets (agronets) herein called eco-friendly nets (EFNs) and floating row covers (FRCs) on microclimate modification, yield, and quality of tomato. A randomized complete block design with five replications was used. Tomato plants were grown under fine mesh EFN (0.4-mm pore diameter) cover, large mesh EFN (0.9-mm pore diameter) cover or FRC. The EFN and FRC were maintained either permanently closed or opened thrice a week from 9 am to 3 pm. Two open control treatments were used: unsprayed (untreated control) or sprayed with chemicals (treated control). The use of EFN or FRC modified the microclimate with higher temperatures, lower diurnal temperature ranges, and higher volumetric water content recorded compared with the controls. On the other hand, light quantity and photosynthetic active radiation were reduced by the use of EFN and FRC compared with the controls. The use of FRC and EFN resulted in more fruit and higher percent in marketable yield compared with open field production. Fruit quality at harvest was also significantly improved by the use of EFN and FRC. Fruits with higher total soluble solids (TSS), lower titratable acidity (TA), and higher sugar acid ratio were obtained in EFN and FRC treatments compared with the controls. Fruits harvested from EFN and FRC were also firmer compared with control fruits. These findings demonstrate the potential of EFN and FRC in modifying microclimate conditions and improving yields and quality of tomato under tropical field conditions.
文摘The effects of Nd^3＋ on the quality and quantity of volatile organic compounds （VOCs） in the leaves of Lycopersicon esculentum were studied. The results demonstrate that Nd^3＋ can increase the total amount of VOC by 75% after treatment for 120 h, as compared with the control. Phyto-oxylipins, terpenoids and aromatic compounds were increased by 73%, 38% and 21%, respectively. （E）-2-hexenal, the most abundant constituent is increased by 74%, β- phellandrene and α-caryophyllene in terpenoids,
