We studied gas-exchange, chlorophyll pigments, lipid peroxidation, antioxidant enzymes, and biomass partitioning responses in seedlings of four wolfberry species （Lycium chinense Mill. var. potaninii （Pojark.） A. M...We studied gas-exchange, chlorophyll pigments, lipid peroxidation, antioxidant enzymes, and biomass partitioning responses in seedlings of four wolfberry species （Lycium chinense Mill. var. potaninii （Pojark.） A. M. Lu, Lycium chinense Mill., Lycium barbarum L., and Lycium yunnanense Kuang ＆ A. M. Lu） under four water supply regimes. In all four species, drought affected seedlings in terms of chlorophyll content, net photosynthesis rate （Pn）＇ transpiration rate （E）, and lipid peroxidation. Drought also increased some antioxidant enzyme activities, such as peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD）, and ascorbate peroxidase （APX）. Significant changes in dry biomass partitioning also occurred in response to water stress. In particular, dry biomass of leaves and fruits decreased significantly. L. chinense Mill. and L. barbarum L. possessed greater drought tolerance and exhibited superior antioxidant processing ability and other related physiological traits compared to the other two species. L. chinense Mill. was the most tolerant to all levels of drought. In contrast,L, yunnanense Kuang ＆ A. M. Lu was more affected by water supply and had the lowest resistance to drought stress. These findings would provide some important information regarding genetic resources for future forest tree improvement in relation to drought tolerance.展开更多
[ Objective ] The study aimed to identify Lycium Linn. at molecular level. [ Method ] The nrDNA ITS sequence of 5 edible Lycium Linn. germplasm resources were investigated. [ Result ] The nrDNA ITS regions of five edi...[ Objective ] The study aimed to identify Lycium Linn. at molecular level. [ Method ] The nrDNA ITS sequence of 5 edible Lycium Linn. germplasm resources were investigated. [ Result ] The nrDNA ITS regions of five edible Lycium Liun. germplasm resources were sequenced. The whole sequences varied from 628 bp to 632 bp, with the average length of 630 bp. Total 79 variation sites were observed in the sequences, which accounts for 12. 5%. [ Conclusion] Sequence analysis based on nrDNA sequencing provides a new approach to identify edible Lycium Linn. germplasm resources.展开更多
[Objective] The study aimed to investigate the genetic polymorphism of eighteen Lycium barbarum resources via nrDNA ITS sequencing. [Method] The genomic DNAs from Lycium barbarum leaves were isolated by modified CTAB ...[Objective] The study aimed to investigate the genetic polymorphism of eighteen Lycium barbarum resources via nrDNA ITS sequencing. [Method] The genomic DNAs from Lycium barbarum leaves were isolated by modified CTAB method for PCR amplification on the nrDNA ITS region using specifically synthesized primers; the amplified fragments were cloned and sequenced, then the sequencing results were clustered. [Result] nrDNA ITS sequences of the tested eighteen Lycium barbarum were firstly obtained in the present study. For all eighteen tested materials, the variation range of whole ITS region was 559-634 bp, with an average of 612 bp; alignment analyses showed that the whole length of internal transcribed spacer (ITS1+ITS2) was 480 bp, within which there are 194 variation sites (accounting for 40.4%) and 286 conserved sites (accounting for 59.6%). The cluster results showed that the eighteen tested materials could be grouped into three classes. [Conclusion] Analysis of nrDNA ITS sequence may avail to identify the Lycium barbarum germplasm resources.展开更多
基金supported by the National Natural Science Foundation of China (31360191, 31660220)the Natural Science Foundation of Ningxia Hui Autonomous Region, China (NZ16121)the Self-option and Foundation of Ningxia Academy of Agriculture and Forestry Sciences, China (YES-16-0402, NKYZ-16-0402)
文摘We studied gas-exchange, chlorophyll pigments, lipid peroxidation, antioxidant enzymes, and biomass partitioning responses in seedlings of four wolfberry species （Lycium chinense Mill. var. potaninii （Pojark.） A. M. Lu, Lycium chinense Mill., Lycium barbarum L., and Lycium yunnanense Kuang ＆ A. M. Lu） under four water supply regimes. In all four species, drought affected seedlings in terms of chlorophyll content, net photosynthesis rate （Pn）＇ transpiration rate （E）, and lipid peroxidation. Drought also increased some antioxidant enzyme activities, such as peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD）, and ascorbate peroxidase （APX）. Significant changes in dry biomass partitioning also occurred in response to water stress. In particular, dry biomass of leaves and fruits decreased significantly. L. chinense Mill. and L. barbarum L. possessed greater drought tolerance and exhibited superior antioxidant processing ability and other related physiological traits compared to the other two species. L. chinense Mill. was the most tolerant to all levels of drought. In contrast,L, yunnanense Kuang ＆ A. M. Lu was more affected by water supply and had the lowest resistance to drought stress. These findings would provide some important information regarding genetic resources for future forest tree improvement in relation to drought tolerance.
基金Natural Science Foundation of Ningxia Hui Autonomous Region(NZ0769)~~
文摘[ Objective ] The study aimed to identify Lycium Linn. at molecular level. [ Method ] The nrDNA ITS sequence of 5 edible Lycium Linn. germplasm resources were investigated. [ Result ] The nrDNA ITS regions of five edible Lycium Liun. germplasm resources were sequenced. The whole sequences varied from 628 bp to 632 bp, with the average length of 630 bp. Total 79 variation sites were observed in the sequences, which accounts for 12. 5%. [ Conclusion] Sequence analysis based on nrDNA sequencing provides a new approach to identify edible Lycium Linn. germplasm resources.
文摘[Objective] The study aimed to investigate the genetic polymorphism of eighteen Lycium barbarum resources via nrDNA ITS sequencing. [Method] The genomic DNAs from Lycium barbarum leaves were isolated by modified CTAB method for PCR amplification on the nrDNA ITS region using specifically synthesized primers; the amplified fragments were cloned and sequenced, then the sequencing results were clustered. [Result] nrDNA ITS sequences of the tested eighteen Lycium barbarum were firstly obtained in the present study. For all eighteen tested materials, the variation range of whole ITS region was 559-634 bp, with an average of 612 bp; alignment analyses showed that the whole length of internal transcribed spacer (ITS1+ITS2) was 480 bp, within which there are 194 variation sites (accounting for 40.4%) and 286 conserved sites (accounting for 59.6%). The cluster results showed that the eighteen tested materials could be grouped into three classes. [Conclusion] Analysis of nrDNA ITS sequence may avail to identify the Lycium barbarum germplasm resources.
