Genetic<span style="font-family:;" "=""><span style="font-family:Verdana;"> variation between naturally occurring ecotypes can be helpful to elucidate the mechanism contr...Genetic<span style="font-family:;" "=""><span style="font-family:Verdana;"> variation between naturally occurring ecotypes can be helpful to elucidate the mechanism controlling salt stress response in different environments. The salt stress response of 20 natural accessions or cultivars of </span><i><span style="font-family:Verdana;">Lycium</span></i><span style="font-family:Verdana;"> was characterized at a physiological level, following exposed to 300 mM NaCl for 15 days. It is shown that salt stress increased Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">, betaine and malondialdehyde (MDA) content, as well as the relative permeability of plasma membranes, but decreased K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">, chlorophyll and glutathione (GSH) content, as well as K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">/Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> ratio compared with their respective control value in most of the varieties, though the increasing or decreasing extent has a clear genetic diversity. The physiological response was utilized to evaluate the salt-tolerant capacity of these varieties by principal component analysis and clustering analysis, and in which total chlorophyll, K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">/Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> ratio and partially GSH have the maximum weight relatively. These varieties could be categorized into four sub-groups, means greatly resistance (Heiguo, Ninqi No. 2 and Beifang), moderately resistant, sensitive, and mostly sensitive (Ninqi No. 7) to salt stress. Our results provide a theoretical basis and practical guidance for the </span><span><span style="font-family:Verdana;">improvement of saline-alkali land and rational utilization of </span><i><span style="font-family:Verdana;">Lycium</span></i></span><span style="font-family:Verdana;"> germplasm resources in the future.</span></span>展开更多
目的:分析不同来源野生黑果枸杞遗传差异和亲缘关系,为黑果枸杞资源鉴定和有效利用提供科学依据。方法:采用SSR分子标记对西北四省区22份野生枸杞种质材料进行鉴定,利用POPGENE 1.32和NTSYS-pc 2.10生物信息学软件对数据进行处理,分析...目的:分析不同来源野生黑果枸杞遗传差异和亲缘关系,为黑果枸杞资源鉴定和有效利用提供科学依据。方法:采用SSR分子标记对西北四省区22份野生枸杞种质材料进行鉴定,利用POPGENE 1.32和NTSYS-pc 2.10生物信息学软件对数据进行处理,分析黑果枸杞种质遗传多样性,计算遗传相似系数,绘制聚类图。结果:筛选出多态性SSR引物37对,在22个样品中共检测到233个基因位点,其中多态性位点213个,多态性比率(Polymorphism ratio,P)达91.4%。材料间遗传相似系数(Genetic similarity,GS)在0.4831~0.9364之间,平均有效等位基因数(Effective number of alleles,Ne)为1.3314,多样性指数(Nei’s gene diversity,H)为0.2106,Shannon信息指数(Shannon’s information index,I)为0.3384。聚类分析(Unweighted pair group of arithmetic means,UPGMA)显示,在相似系数为0.61和0.77处,可将22个材料分为2大类和4个亚类,不同种类的枸杞种质材料可完全分类。结论:供试枸杞种质材料间遗传多样性较为丰富,不同种类枸杞对系统聚类结果影响较大,但种内材料地域分布规律性不明显。展开更多
目的利用荧光标记辅助的扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术,从基因组DNA水平上分析我国6个栽培生产区域52份枸杞Lycium chinense样本的遗传多样性。方法对提取的枸杞DNA样本进行酶切、连接、预扩增...目的利用荧光标记辅助的扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术,从基因组DNA水平上分析我国6个栽培生产区域52份枸杞Lycium chinense样本的遗传多样性。方法对提取的枸杞DNA样本进行酶切、连接、预扩增和选扩增等步骤,扩增产物经毛细管电泳系统分离和数据采集后,利用Popgene软件计算遗传多样性参数,利用GenAlex软件计算遗传距离并进行主坐标分析,利用MEGA-X软件根据遗传距离进行聚类分析,利用Structure软件进行群体遗传结构分析。结果共筛选得到10对AFLP选择性扩增引物组合,扩增得到328条扩增片段,其中多态性片段121条,且在不同产地枸杞居群间多态性位点分布不均匀。分析显示不同栽培产区枸杞的遗传多样性较低,等位基因数(observed number of alleles,Na)、有效等位基因数(effective number of alleles,Ne)、观测杂合度(Nei’s gene diversity,H)和香浓信息指数(Shannon information index,I)分别为1.4380、1.2316、0.1407和0.2152,Nei’s遗传相似性范围为0.8354~0.8902。结论不同栽培生产区域的枸杞居群间存在较为频繁的基因交流,遗传分化程度中等。居群内部遗传变异是枸杞遗传变异的主要来源,居群间遗传变异不显著,同时遗传距离与地理距离没有明显相关性。可为枸杞的遗传背景信息积累、引种栽培、种质资源保护、核心种质库构建以及可持续开发策略的制定和管理提供科学依据。展开更多
文摘Genetic<span style="font-family:;" "=""><span style="font-family:Verdana;"> variation between naturally occurring ecotypes can be helpful to elucidate the mechanism controlling salt stress response in different environments. The salt stress response of 20 natural accessions or cultivars of </span><i><span style="font-family:Verdana;">Lycium</span></i><span style="font-family:Verdana;"> was characterized at a physiological level, following exposed to 300 mM NaCl for 15 days. It is shown that salt stress increased Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">, betaine and malondialdehyde (MDA) content, as well as the relative permeability of plasma membranes, but decreased K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">, chlorophyll and glutathione (GSH) content, as well as K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">/Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> ratio compared with their respective control value in most of the varieties, though the increasing or decreasing extent has a clear genetic diversity. The physiological response was utilized to evaluate the salt-tolerant capacity of these varieties by principal component analysis and clustering analysis, and in which total chlorophyll, K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;">/Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> ratio and partially GSH have the maximum weight relatively. These varieties could be categorized into four sub-groups, means greatly resistance (Heiguo, Ninqi No. 2 and Beifang), moderately resistant, sensitive, and mostly sensitive (Ninqi No. 7) to salt stress. Our results provide a theoretical basis and practical guidance for the </span><span><span style="font-family:Verdana;">improvement of saline-alkali land and rational utilization of </span><i><span style="font-family:Verdana;">Lycium</span></i></span><span style="font-family:Verdana;"> germplasm resources in the future.</span></span>
文摘目的:分析不同来源野生黑果枸杞遗传差异和亲缘关系,为黑果枸杞资源鉴定和有效利用提供科学依据。方法:采用SSR分子标记对西北四省区22份野生枸杞种质材料进行鉴定,利用POPGENE 1.32和NTSYS-pc 2.10生物信息学软件对数据进行处理,分析黑果枸杞种质遗传多样性,计算遗传相似系数,绘制聚类图。结果:筛选出多态性SSR引物37对,在22个样品中共检测到233个基因位点,其中多态性位点213个,多态性比率(Polymorphism ratio,P)达91.4%。材料间遗传相似系数(Genetic similarity,GS)在0.4831~0.9364之间,平均有效等位基因数(Effective number of alleles,Ne)为1.3314,多样性指数(Nei’s gene diversity,H)为0.2106,Shannon信息指数(Shannon’s information index,I)为0.3384。聚类分析(Unweighted pair group of arithmetic means,UPGMA)显示,在相似系数为0.61和0.77处,可将22个材料分为2大类和4个亚类,不同种类的枸杞种质材料可完全分类。结论:供试枸杞种质材料间遗传多样性较为丰富,不同种类枸杞对系统聚类结果影响较大,但种内材料地域分布规律性不明显。
文摘目的利用荧光标记辅助的扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术,从基因组DNA水平上分析我国6个栽培生产区域52份枸杞Lycium chinense样本的遗传多样性。方法对提取的枸杞DNA样本进行酶切、连接、预扩增和选扩增等步骤,扩增产物经毛细管电泳系统分离和数据采集后,利用Popgene软件计算遗传多样性参数,利用GenAlex软件计算遗传距离并进行主坐标分析,利用MEGA-X软件根据遗传距离进行聚类分析,利用Structure软件进行群体遗传结构分析。结果共筛选得到10对AFLP选择性扩增引物组合,扩增得到328条扩增片段,其中多态性片段121条,且在不同产地枸杞居群间多态性位点分布不均匀。分析显示不同栽培产区枸杞的遗传多样性较低,等位基因数(observed number of alleles,Na)、有效等位基因数(effective number of alleles,Ne)、观测杂合度(Nei’s gene diversity,H)和香浓信息指数(Shannon information index,I)分别为1.4380、1.2316、0.1407和0.2152,Nei’s遗传相似性范围为0.8354~0.8902。结论不同栽培生产区域的枸杞居群间存在较为频繁的基因交流,遗传分化程度中等。居群内部遗传变异是枸杞遗传变异的主要来源,居群间遗传变异不显著,同时遗传距离与地理距离没有明显相关性。可为枸杞的遗传背景信息积累、引种栽培、种质资源保护、核心种质库构建以及可持续开发策略的制定和管理提供科学依据。
