The total RNA is extracted from leaf and bulb of Lycoris radiata(L’Hér.)Herb.by an improved method of CsCl density gradient centrifugation.The suitable amount of initial sample is 1.0 g.In order to remove impuri...The total RNA is extracted from leaf and bulb of Lycoris radiata(L’Hér.)Herb.by an improved method of CsCl density gradient centrifugation.The suitable amount of initial sample is 1.0 g.In order to remove impurities and purify the total RNA,the crude extract of RNA dissolved in lysis buffer is extracted with the mixed solution of chloroform and isoamyl alcohol(V ∶V,24 ∶1),then the total RNA is precipitated.The purity,integrity and quality of the total RNA are detected by ultraviolet spectrophotometer,agarose gel electrophoresis and RT-PCR method,respectively.The results show that polysaccharide can be removed effectively from the total RNA extract by the improved method of CsCl density gradient centrifugation.The purity andintegrityof the total RNA of L.radiata are better,and the ratio of A260/A280 is 1.85-2.00.It is concluded that the total RNA of L.radiata bulb extracted by the improved method can satisfy the demand of molecular biology experiments.展开更多
在栽植前采用0(CK)、60、120和180 mg·L-16-BA、GA3和乙烯利对石蒜〔Lycoris radiata(L’Hér.)Herb.〕和换锦花(L.sprengeri Comes ex Baker)鳞茎进行浸球处理,对花芽分化期内(4月至7月)石蒜和换锦花鳞茎中可溶性糖、可溶性...在栽植前采用0(CK)、60、120和180 mg·L-16-BA、GA3和乙烯利对石蒜〔Lycoris radiata(L’Hér.)Herb.〕和换锦花(L.sprengeri Comes ex Baker)鳞茎进行浸球处理,对花芽分化期内(4月至7月)石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量的变化进行了比较分析。结果表明:在花芽分化期内,随时间的推移,对照组石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量呈先增加后降低的趋势,并在6月25日(心皮分化期)或7月10日(雌蕊分化期)达到最高值。经60、120和180 mg·L-16-BA、GA3和乙烯利浸球处理后,石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量在花芽分化期内先逐渐增加,至6月25日或达到峰值后开始下降或继续升高;但与对照相比,随时间的推移,石蒜鳞茎中可溶性糖含量总体上有不同程度的增加,而换锦花鳞茎中可溶性糖含量或高或低无规律性的变化,但各处理组石蒜和换锦花鳞茎中可溶性糖含量均与对照间有差异;可溶性蛋白质含量在进入花原基形成期(5月10日)后始终维持在较高的水平;核酸含量在花芽分化期的前期维持在较低的水平,在雌蕊分化期迅速增加并达到峰值,之后又急剧下降。总体上看,石蒜鳞茎中可溶性糖含量高于换锦花,可溶性蛋白质含量低于换锦花,而二者间核酸含量差异不明显。研究结果表明:3种外源植物生长调节剂浸球处理可在一定程度上对花芽分化期内石蒜和换锦花鳞茎的新陈代谢产生影响,且对石蒜的影响强于换锦花。展开更多
文摘The total RNA is extracted from leaf and bulb of Lycoris radiata(L’Hér.)Herb.by an improved method of CsCl density gradient centrifugation.The suitable amount of initial sample is 1.0 g.In order to remove impurities and purify the total RNA,the crude extract of RNA dissolved in lysis buffer is extracted with the mixed solution of chloroform and isoamyl alcohol(V ∶V,24 ∶1),then the total RNA is precipitated.The purity,integrity and quality of the total RNA are detected by ultraviolet spectrophotometer,agarose gel electrophoresis and RT-PCR method,respectively.The results show that polysaccharide can be removed effectively from the total RNA extract by the improved method of CsCl density gradient centrifugation.The purity andintegrityof the total RNA of L.radiata are better,and the ratio of A260/A280 is 1.85-2.00.It is concluded that the total RNA of L.radiata bulb extracted by the improved method can satisfy the demand of molecular biology experiments.
文摘在栽植前采用0(CK)、60、120和180 mg·L-16-BA、GA3和乙烯利对石蒜〔Lycoris radiata(L’Hér.)Herb.〕和换锦花(L.sprengeri Comes ex Baker)鳞茎进行浸球处理,对花芽分化期内(4月至7月)石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量的变化进行了比较分析。结果表明:在花芽分化期内,随时间的推移,对照组石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量呈先增加后降低的趋势,并在6月25日(心皮分化期)或7月10日(雌蕊分化期)达到最高值。经60、120和180 mg·L-16-BA、GA3和乙烯利浸球处理后,石蒜和换锦花鳞茎中可溶性糖、可溶性蛋白质和核酸含量在花芽分化期内先逐渐增加,至6月25日或达到峰值后开始下降或继续升高;但与对照相比,随时间的推移,石蒜鳞茎中可溶性糖含量总体上有不同程度的增加,而换锦花鳞茎中可溶性糖含量或高或低无规律性的变化,但各处理组石蒜和换锦花鳞茎中可溶性糖含量均与对照间有差异;可溶性蛋白质含量在进入花原基形成期(5月10日)后始终维持在较高的水平;核酸含量在花芽分化期的前期维持在较低的水平,在雌蕊分化期迅速增加并达到峰值,之后又急剧下降。总体上看,石蒜鳞茎中可溶性糖含量高于换锦花,可溶性蛋白质含量低于换锦花,而二者间核酸含量差异不明显。研究结果表明:3种外源植物生长调节剂浸球处理可在一定程度上对花芽分化期内石蒜和换锦花鳞茎的新陈代谢产生影响,且对石蒜的影响强于换锦花。
