Objective: To investigate the pattern of clonal rearrangement of immunoglobulin heavy chain gene (IGH) and T cell receptor γ gene (TCRγ) of Non Hodgkin's lymphoma (NHL) Methods: Bone marrow smears of 211 pat...Objective: To investigate the pattern of clonal rearrangement of immunoglobulin heavy chain gene (IGH) and T cell receptor γ gene (TCRγ) of Non Hodgkin's lymphoma (NHL) Methods: Bone marrow smears of 211 patients of NHL were detected by PCR, the rearranged IGH and TCRγ gene was amplified using oligonucleotide primers Results: The clonal rearrangement of IGH gene was detectable in 51 2% (108/211); the clonal rearrangement of TCRγ gene was detectable in 21 3% (45/211); both IGH and TCRγ was detectable in 5 7% (12/211); no clonal rearrangement in 21 8% (46/211) And compared clonal gene rearrangement with pathological type and primary site of tumor Ten patients of NHL were investigated serially 5/10 patients still had clonal gene rearrangement at clinical complete remission Conclusion: It demonstrated that this assay may be useful in monitoring the minimal residual disease (MRD) and in evaluating effectiveness of therapy展开更多
Lymph node aspirates of 17 cases with enlarged superficial or isceral lymph nodes were detected for immunoglobulin heavy chain gene rearrangement (IgHRA) and T cell rcceptor γ gene rearrangement (TCRγRA) bypolymeras...Lymph node aspirates of 17 cases with enlarged superficial or isceral lymph nodes were detected for immunoglobulin heavy chain gene rearrangement (IgHRA) and T cell rcceptor γ gene rearrangement (TCRγRA) bypolymerase chain reaction (PCR). Combining with clinical data, pathologic diagnosis and immunophenotapy, we analyse the results as follows: 5 cases with nonlymphoid cancers and 3 cases with reactive lymphadenopathy do not present two kinds of clone gene rearrangements.5 out of 7 cases with NHL show clone gene rearrangements (IgH 3 cases, TCRY 2cases),two kinds of monoclonal band(100-120bp for IgHRA and 170-230bp for TCRγRA) were observed after electrophoresis of amplified DNA products. One case whose clinical sitcaion accorded with features of lymphoma was diagnosed as granulomatous lymphadenitis by pathologist, after gene rearaangement clone TCRγRA was detected,a correct diaguosis as NHL was made then. The significance of detecting the two kinds of gene rearrangement for clinical application and the limitations in diagnosis of lymphoproliferative disorders was discussed.展开更多
By means of a series of monoclonal antibodies, gene probes of immanoglobulin aheavy chain (IgH), T cell receptor (TCR) gamma and delta chain as well as chromosome karyotype analysis, the immunophenotype and karyotype ...By means of a series of monoclonal antibodies, gene probes of immanoglobulin aheavy chain (IgH), T cell receptor (TCR) gamma and delta chain as well as chromosome karyotype analysis, the immunophenotype and karyotype were studied in 23 children with acute lymphoblastic leukemia (ALL) diagnosed according to FAB classification criteria and genotype in 21 of them. Among them 16 cases belonged to B lineage ALL, 4 cases to T-ALL, one case to ANLL, and 2 cases to acute undifferential leukemia (AUL). The IgH gene rearrangement was expressed in all of the B lineage ALL, the T-ALL were in germline. The gene rearrangement of TCR gamma and delta chain was expressed in both type of ALL, but the form of rearrangement was different. The quantitative abnormalities of chromosomes were found in 11 of 23 cases, 2 cases had chromosome structure abnormalities. Correlating with clinical manifestations it was found that some relationship between morphology (M), immunology (I), cytogeneties (C), genotype and clinical manifestations in type B-Ⅳ, T-ALL, and A UL exieted, indicating that they had great significance in refining subclassification, prognosis and guidance of treatment in childhood ALLs.展开更多
文摘Objective: To investigate the pattern of clonal rearrangement of immunoglobulin heavy chain gene (IGH) and T cell receptor γ gene (TCRγ) of Non Hodgkin's lymphoma (NHL) Methods: Bone marrow smears of 211 patients of NHL were detected by PCR, the rearranged IGH and TCRγ gene was amplified using oligonucleotide primers Results: The clonal rearrangement of IGH gene was detectable in 51 2% (108/211); the clonal rearrangement of TCRγ gene was detectable in 21 3% (45/211); both IGH and TCRγ was detectable in 5 7% (12/211); no clonal rearrangement in 21 8% (46/211) And compared clonal gene rearrangement with pathological type and primary site of tumor Ten patients of NHL were investigated serially 5/10 patients still had clonal gene rearrangement at clinical complete remission Conclusion: It demonstrated that this assay may be useful in monitoring the minimal residual disease (MRD) and in evaluating effectiveness of therapy
文摘Lymph node aspirates of 17 cases with enlarged superficial or isceral lymph nodes were detected for immunoglobulin heavy chain gene rearrangement (IgHRA) and T cell rcceptor γ gene rearrangement (TCRγRA) bypolymerase chain reaction (PCR). Combining with clinical data, pathologic diagnosis and immunophenotapy, we analyse the results as follows: 5 cases with nonlymphoid cancers and 3 cases with reactive lymphadenopathy do not present two kinds of clone gene rearrangements.5 out of 7 cases with NHL show clone gene rearrangements (IgH 3 cases, TCRY 2cases),two kinds of monoclonal band(100-120bp for IgHRA and 170-230bp for TCRγRA) were observed after electrophoresis of amplified DNA products. One case whose clinical sitcaion accorded with features of lymphoma was diagnosed as granulomatous lymphadenitis by pathologist, after gene rearaangement clone TCRγRA was detected,a correct diaguosis as NHL was made then. The significance of detecting the two kinds of gene rearrangement for clinical application and the limitations in diagnosis of lymphoproliferative disorders was discussed.
文摘By means of a series of monoclonal antibodies, gene probes of immanoglobulin aheavy chain (IgH), T cell receptor (TCR) gamma and delta chain as well as chromosome karyotype analysis, the immunophenotype and karyotype were studied in 23 children with acute lymphoblastic leukemia (ALL) diagnosed according to FAB classification criteria and genotype in 21 of them. Among them 16 cases belonged to B lineage ALL, 4 cases to T-ALL, one case to ANLL, and 2 cases to acute undifferential leukemia (AUL). The IgH gene rearrangement was expressed in all of the B lineage ALL, the T-ALL were in germline. The gene rearrangement of TCR gamma and delta chain was expressed in both type of ALL, but the form of rearrangement was different. The quantitative abnormalities of chromosomes were found in 11 of 23 cases, 2 cases had chromosome structure abnormalities. Correlating with clinical manifestations it was found that some relationship between morphology (M), immunology (I), cytogeneties (C), genotype and clinical manifestations in type B-Ⅳ, T-ALL, and A UL exieted, indicating that they had great significance in refining subclassification, prognosis and guidance of treatment in childhood ALLs.
