Objective： Recent studies have suggested that t（14;18） is present in a significant proportion of diffuse large B-cell lymphomas （DLBCLs）. However, the prognostic significance of this translocation and its relatio...Objective： Recent studies have suggested that t（14;18） is present in a significant proportion of diffuse large B-cell lymphomas （DLBCLs）. However, the prognostic significance of this translocation and its relationship with BCL-2 protein expression remains controversial. Our study aimed to investigate the predictive power of t（14;18） and BCL-2 protein expression in the prognosis of DLBCLs. Methods： Biopsy specimens from 106 DLBCLs were analyzed using interphase fluorescence in situ hybridization （FISH）. Immunophenotypic analysis of CD20, CD3, CD10, BCL-6, MUM1 and BCL-2 was performed by immunohistochemistry. SPSS 13.0 software was used for statistical analysis. Results： The t（14;18） was identified in 27 of 106 cases （25.5%）. The percentages of tumor cells expressing CD10, BCL-6, MUM1 and BCL-2 were 21.7%, 26.4%, 56.6% and 73.6%, respectively. The presence of this translocation was significantly correlated with the expression of CD10 and immunophenotypic subtype （p0.001）. No association was observed between BCL-2 protein expression and the presence of t（14;18）. Multivariate analysis confirmed that both t（14;18） and BCL-2 expression were significantly associated with survival. Moreover, patients with t（14;18） had worse prognosis, compared with those with BCL-2 expression （for overall survival： hazard ratio, 4.235; 95%CI, 2.153-8.329, p0.001 vs. hazard ration, 2.743; 95%CI, 1.262-5.962, p=0.011）. Conclusions： The t（14;18） is a useful prognostic tool for the evaluation of DLBCL immunophenotype and prognosis. The prognosis of GCB （germinal centre-like B cell） DLBCL patients should be made with the consideration of the presence of this translocation, and the detection of t（14;18） should be included as a routine diagnostic test in these cases.展开更多
The aim of the present study was to explore the differentially expressed genes in the blood vessel endothelial cells （BVECs） between diffuse large B-cell lymphoma （DLBCL） and reactive lymph node hyperplasia （RLNH...The aim of the present study was to explore the differentially expressed genes in the blood vessel endothelial cells （BVECs） between diffuse large B-cell lymphoma （DLBCL） and reactive lymph node hyperplasia （RLNH）, and to perform an initial bioinformatics analysis on a novel gene, C20orf14, which is highly expressed in lymph node of lymphoma. The mRNA of the tissue from the BVECs of DLBCL and RLNH tissues was labeled with biotin respectively and hybridized with expression profile microarray, and the differentially expressed genes were obtained. Initial bio-informatics analysis was performed on a novel gene named C20orf14. Its gene structure, genomic localization, the physical and chemical characteristics of the putative protein, subcellular localization, functional domain etc. were predicted, and the systematic evolution analysis was performed on the similar proteins among several species. By using expression profile microarray, many differentially expressed genes were uncovered. The efficient bioinformatics analysis have fundamentally identified that C20orf14 was a nuclear protein, and may be involved in the post-transcription modification of mRNA. Therefore, microarray is an efficient and high throughout strategy for the detection of differentially expressed genes, and C20orf14 is thought to be a potential target for tumor metastasis researches by bioinformatics analysis.展开更多
通过采用电弧炉出钢前在钢包中加入40%的锰铁,LF精炼前期调整成分,精炼中期和末期控制精炼渣碱度≥2.5,软吹氩搅拌,控制中间包钢水过热度≤35℃,连铸全程进行保护浇铸等工艺措施,贵阳特钢—炼钢厂成功进行了80Mn14钢(%:0.75～0.96C、13...通过采用电弧炉出钢前在钢包中加入40%的锰铁,LF精炼前期调整成分,精炼中期和末期控制精炼渣碱度≥2.5,软吹氩搅拌,控制中间包钢水过热度≤35℃,连铸全程进行保护浇铸等工艺措施,贵阳特钢—炼钢厂成功进行了80Mn14钢(%:0.75～0.96C、13.00～15.50Mn、≤0.80Si、≤0.070P、≤0.030S)的30 t EBT AF-35 t LF-260mm×300mm连铸机流程生产,产品各项性能均满足标准要求。展开更多
文摘Objective： Recent studies have suggested that t（14;18） is present in a significant proportion of diffuse large B-cell lymphomas （DLBCLs）. However, the prognostic significance of this translocation and its relationship with BCL-2 protein expression remains controversial. Our study aimed to investigate the predictive power of t（14;18） and BCL-2 protein expression in the prognosis of DLBCLs. Methods： Biopsy specimens from 106 DLBCLs were analyzed using interphase fluorescence in situ hybridization （FISH）. Immunophenotypic analysis of CD20, CD3, CD10, BCL-6, MUM1 and BCL-2 was performed by immunohistochemistry. SPSS 13.0 software was used for statistical analysis. Results： The t（14;18） was identified in 27 of 106 cases （25.5%）. The percentages of tumor cells expressing CD10, BCL-6, MUM1 and BCL-2 were 21.7%, 26.4%, 56.6% and 73.6%, respectively. The presence of this translocation was significantly correlated with the expression of CD10 and immunophenotypic subtype （p0.001）. No association was observed between BCL-2 protein expression and the presence of t（14;18）. Multivariate analysis confirmed that both t（14;18） and BCL-2 expression were significantly associated with survival. Moreover, patients with t（14;18） had worse prognosis, compared with those with BCL-2 expression （for overall survival： hazard ratio, 4.235; 95%CI, 2.153-8.329, p0.001 vs. hazard ration, 2.743; 95%CI, 1.262-5.962, p=0.011）. Conclusions： The t（14;18） is a useful prognostic tool for the evaluation of DLBCL immunophenotype and prognosis. The prognosis of GCB （germinal centre-like B cell） DLBCL patients should be made with the consideration of the presence of this translocation, and the detection of t（14;18） should be included as a routine diagnostic test in these cases.
基金grants from National "973" Key Basic Research and Development Program Foundation (No. 2002CB513100) the transversal project of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (No. 2007004) of China
文摘The aim of the present study was to explore the differentially expressed genes in the blood vessel endothelial cells （BVECs） between diffuse large B-cell lymphoma （DLBCL） and reactive lymph node hyperplasia （RLNH）, and to perform an initial bioinformatics analysis on a novel gene, C20orf14, which is highly expressed in lymph node of lymphoma. The mRNA of the tissue from the BVECs of DLBCL and RLNH tissues was labeled with biotin respectively and hybridized with expression profile microarray, and the differentially expressed genes were obtained. Initial bio-informatics analysis was performed on a novel gene named C20orf14. Its gene structure, genomic localization, the physical and chemical characteristics of the putative protein, subcellular localization, functional domain etc. were predicted, and the systematic evolution analysis was performed on the similar proteins among several species. By using expression profile microarray, many differentially expressed genes were uncovered. The efficient bioinformatics analysis have fundamentally identified that C20orf14 was a nuclear protein, and may be involved in the post-transcription modification of mRNA. Therefore, microarray is an efficient and high throughout strategy for the detection of differentially expressed genes, and C20orf14 is thought to be a potential target for tumor metastasis researches by bioinformatics analysis.
文摘通过采用电弧炉出钢前在钢包中加入40%的锰铁,LF精炼前期调整成分,精炼中期和末期控制精炼渣碱度≥2.5,软吹氩搅拌,控制中间包钢水过热度≤35℃,连铸全程进行保护浇铸等工艺措施,贵阳特钢—炼钢厂成功进行了80Mn14钢(%:0.75～0.96C、13.00～15.50Mn、≤0.80Si、≤0.070P、≤0.030S)的30 t EBT AF-35 t LF-260mm×300mm连铸机流程生产,产品各项性能均满足标准要求。
