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细梗香草及近缘种内生真菌的多样性
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作者 林贵兵 吴少华 +1 位作者 刘勇 章常华 《中南农业科技》 2024年第3期40-42,共3页
研究细梗香草(Lysimachia capillipes Hemsl.)及近缘种内生真菌多样性,为开发新微生物药物资源奠定基础。采用4种不同培养基分离不同地点样品内生真菌菌种,提取菌丝DNA,扩增ITS序列,测序后GenBank中Blast对比得到同源相似度较高的菌种... 研究细梗香草(Lysimachia capillipes Hemsl.)及近缘种内生真菌多样性,为开发新微生物药物资源奠定基础。采用4种不同培养基分离不同地点样品内生真菌菌种,提取菌丝DNA,扩增ITS序列,测序后GenBank中Blast对比得到同源相似度较高的菌种。获得分离内生真菌共258株;马铃薯葡萄糖琼脂培养基(PDA)和改良马丁氏培养基(改良MRS)分离内生真菌数量较多,分别占总数的32.56%和28.68%,菌种生长速率较快,而水琼脂(WA)培养基分离菌种数最少,占总数的17.05%,菌落生长速率较慢。相似度较高的菌种有27种,占总菌种数的10.47%。PDA和改良MRS是分离培养细梗香草内生菌较好的培养基,分离得到的菌种重复性较高,4种培养基分离的菌种差异较小。 展开更多
关键词 细梗香草(lysimachia capillipes hemsl.) 近缘种 内生真菌 多样性
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Capilliposide from Lysimachia capillipes promotes terminal differentiations and reverses paclitaxel resistance in A2780T cells of human ovarian cancer by regulating Fos/Jun pathway 被引量:1
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作者 Ke Zhang Hanyue Ying +2 位作者 Ruping Zhao Yuanyuan Chen Qinghua Deng 《Chinese Herbal Medicines》 CAS 2022年第1期111-116,共6页
Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sensitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the... Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sensitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the IC50 values.The apoptosis of cells was measured through flow cytometry.Evaluation of the stemness and differentiation markers was performed by the immunoblotting and the immunostaining assays.RNA-seq was performed through the Illumina HiSeq PE150 platform and differentially expressed genes(DEGs)were screened out through the bioinformation analysis.Overexpression or knockdown of Fos gene was achieved by shRNA transfection.Results:Pre-exposure of A2780T cells with 10μg/mL LCC sensitized them to paclitaxel,of which the IC50 value reduced from 8.644μmol/L(95%CI:7.315–10.082μmol/L)to 2.5μmol/L(95%CI:2.233–2.7882μmol/L).Exposure with LCC enhanced the paclitaxel-induced apoptosis and inhibited the colony formation of A2780T cells.LCC exposure reduced the expression of cancer stemness markers,ALDH1,Myd88 and CD44,while promoting that of terminal differentiation markers,NFATc1,Cathepsin K and MMP9.RNAseq analysis revealed that the expressions of FOS and JUN were upregulated in LCC-treated A2780T cells.A2780T cells overexpressing Fos gene displayed increased paclitaxel-sensitivity and reduced cell stemness,and shared common phenotypes with LCC-treated A2780T cells.Conclusion:These findings suggested that LCC promoted terminal differentiations of ovarian cancer cells and sensitized them to paclitaxel through activating the Fos/Jun pathway.LCC might become a novel therapy that targets at cancer stem cells and enhances the chemotherapeutic effect of ovarian cancer treatments. 展开更多
关键词 cancer stem cell capilliposide chemo-resistance Fos gene lysimachia capillipes hemsl ovarian cancer
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