Two Novel Saponins from Lysimachia capillipes

Two new saponins named capilliposide C 1 and capilliposide D 2 were isolated from the whole plants of Lysimachia capillipes, their structures were deternuned by 1D and 2D NMR, ESIMS techniques, and chemical methods.Capilliposide C showed significant cytotoxic activity against human A2780 cells.

Two New Saponins from Lysimachia capillipes Hemsl.

To investigate the saponins from whole plants of Lysimachia capilUpes Hemsl., two new saponins, named capilliposide E （1） and capilliposide F （2）, were isolated. The structures of the new saponins were elucidated as 3β, 22α-dihydroxy-16α-acetat-28→ 13-lactone-oleanane-3-O- [β-D-glucopyranosyl- （1 →2）-α-L-arabinpyranoyl]-22-O-β-D-glucopyranoside （1） and 3β, 22α-dihydroxy- 16α-acetat-28→ 13-lactone-oleanane-3-O-{ [β-D-glucopyranosyl-（1→2）-β-D-glucopyranosyl-（1→4）]-α-L-arabinpyranoyl}-22-O-β- D-glucopyranoside （2）. The structures of these compounds were determined by 1D- and 2D-NMR, MS techniques, and chemical methods.

细梗香草总皂苷壳聚糖絮凝纯化工艺研究

目的研究细梗香草总皂苷壳聚糖絮凝纯化工艺,为继续纯化三萜皂苷B(LC-B)和三萜皂苷C(LC-C)奠定基础。方法用可见分光光度法、高效液相色谱-蒸发光散射检测法(HPLC-ELSD),分别测定总皂苷中间体水溶液絮凝率、总皂苷损失率,以絮凝率和总皂苷损失率为指标,用正交实验优选壳聚糖絮凝纯化最佳工艺条件。结果最佳工艺条件为总皂苷质量浓度为0.6 mg·mL-1,每100 mL总皂苷水溶液加入壳聚糖溶液30 mL,絮凝温度60℃,絮凝时间4 h。验证结果表明,总皂苷中间体水溶液澄清率为47.81%,总皂苷损失率为9.53%。结论与传统除鞣质工艺相比,壳聚糖絮凝纯化工艺能有效保留有效成分,工艺稳定性好,适合工业化生产。