Background: Extended-spectrum beta-lactamase (ESBL) producing bacteria are a real public health problem, particularly in Africa. Among these ESBLs, there are the Muenchen Cefotaximase (CTX-M) described all over the wo...Background: Extended-spectrum beta-lactamase (ESBL) producing bacteria are a real public health problem, particularly in Africa. Among these ESBLs, there are the Muenchen Cefotaximase (CTX-M) described all over the world of which the most frequent is the CTX-M of group 1 particularly the CTX-M-15 variant. The objective of this study was to determine the prevalence of CTX-M group 1 ESBL-producing Escherichia coli strains and to test their antibiotics susceptibility profile. Methodology: A retrospective cross-sectional descriptive study was conducted to detect ESBL-secreting Escherichia coli strains by the synergy test. Identification of CTX-M type ESBL from group 1 was performed using the NG-Test CTX-M rapid diagnostic test (NG-Biotech®). Antibiotic susceptibility profile was determined using CA-SFM/EUCAST guidelines 2019. Data entry and statistical analysis were performed with Excel version 2010 and SPSS 20.0 respectively. Results: Eighty-two ESBL-producing Escherichia coli strains were tested. A group 1 CTX-M ESBL was detected in 75.6% of the strains (n = 62). These strains were highly resistant to cefotaxim (100%), aztreonam (100%), ceftazidim (85.4%) and cefepim (66.1%). They were also resistant to quinolones, gentamycin and sulfadoxine-trimethoprim combination. However, these strains showed sensitivity to ertapenem (100%), cefoxitin (69.3%), tigecyclin (66%), and amikacin (66.1%). The combination of piperacillin and tazobactam was active on 30.6% of the strains against 6.4% for the combination of amoxicillin and clavulanic acid. Conclusion: The CTX-M type ESBL of group 1 was present in the majority of ESBL-producing Escherichia colis trains. Despite the production of this enzyme conferring resistance to most beta-lactam antibiotics, some antibiotics remain active to treat infections caused by these germs.展开更多
Let S\-n be the symmetric group, g\++\-i=(123i),g\+-\-i=(1i32) and M\++\-n={g\++\-i∶4≤i≤n}, then M\++\-n is a minimal generating set of S\-n ,where n ≥5.It is proved that Cayley graph Cay( S\-...Let S\-n be the symmetric group, g\++\-i=(123i),g\+-\-i=(1i32) and M\++\-n={g\++\-i∶4≤i≤n}, then M\++\-n is a minimal generating set of S\-n ,where n ≥5.It is proved that Cayley graph Cay( S\-n,M\++\-n∪M\+-\-n) is Hamiltonian and edge symmetric.展开更多
Analytic atlases on <img src="Edit_948e45b7-cbef-425e-bb79-28648b859994.png" width="23" height="22" alt="" /> can be easily defined making it an <em>n</em>-dim...Analytic atlases on <img src="Edit_948e45b7-cbef-425e-bb79-28648b859994.png" width="23" height="22" alt="" /> can be easily defined making it an <em>n</em>-dimensional complex manifold. Then with the help of bi-M<span style="white-space:nowrap;"><span style="white-space:nowrap;">ö</span></span>bius transformations in complex coordinates Abelian groups are constructed making this manifold a Lie group. Actions of Lie groups on differentiable manifolds are well known and serve different purposes. We have introduced in previous works actions of Lie groups on non orientable Klein surfaces. The purpose of this work is to extend those studies to non orientable <em>n</em>-dimensional complex manifolds. Such manifolds are obtained by factorizing <img src="Edit_7e5721ee-bb7f-4224-bd52-7d4641ac1c73.png" width="23" height="22" alt="" /> with the two elements group of a fixed point free antianalytic involution of <img src="Edit_ddfdac64-b296-48c5-9bb2-932eb3d76008.png" width="23" height="22" alt="" />. Involutions <strong>h(z)</strong> of this kind are obtained linearly by composing special M<span style="white-space:nowrap;"><span style="white-space:nowrap;">ö</span></span>bius transformations of the planes with the mapping <img src="Edit_4cda269a-9399-41ae-a5da-0c9d18a419ab.png" width="89" height="24" alt="" /><img src="Edit_4cda269a-9399-41ae-a5da-0c9d18a419ab.png" width="85" height="22" alt="" />. A convenient partition of <img src="Edit_9e899708-41b0-4351-a12b-cc6efb5b1581.png" width="23" height="22" alt="" /> is performed which helps defining an internal operation on <img src="Edit_7cd42987-68f8-4e4c-9382-cbc68b56377e.png" width="49" height="26" alt="" /> and finally actions of the previously defined Lie groups on the non orientable manifold <img src="Edit_5740b48c-f9ea-438d-a87d-8cdc1f83662b.png" width="49" height="25" alt="" /> are displayed.展开更多
Background: Sepsis involves life-threatening organ dysfunction and is caused by a dysregulated host response to infection. No specific therapies against sepsis have been reported. Celastrol(Cel) is a natural anti-infl...Background: Sepsis involves life-threatening organ dysfunction and is caused by a dysregulated host response to infection. No specific therapies against sepsis have been reported. Celastrol(Cel) is a natural anti-inflammatory compound that shows potential against systemic inflammatory diseases. This study aimed to investigate the pharmacological activity and molecular mechanism of Cel in models of endotoxemia and sepsis.Methods: We evaluated the anti-inflammatory efficacy of Cel against endotoxemia and sepsis in mice and macrophage cultures treated with lipopolysaccharide(LPS). We screened for potential protein targets of Cel using activity-based protein profiling(ABPP). Potential targets were validated using biophysical methods such as cellular thermal shift assays(CETSA) and surface plasmon resonance(SPR). Residues involved in Cel binding to target proteins were identified through point mutagenesis, and the functional effects of such binding were explored through gene knockdown.Results: Cel protected mice from lethal endotoxemia and improved their survival with sepsis, and it significantly decreased the levels of pro-inflammatory cytokines in mice and macrophages treated with LPS(P <0.05). Cel bound to Cys424 of pyruvate kinase M2(PKM2), inhibiting the enzyme and thereby suppressing aerobic glycolysis(Warburg effect). Cel also bound to Cys106 in high mobility group box 1(HMGB1) protein, reducing the secretion of inflammatory cytokine interleukin(IL)-1β. Cel bound to the Cys residues in lactate dehydrogenase A(LDHA).Conclusions: Cel inhibits inflammation and the Warburg effect in sepsis via targeting PKM2 and HMGB1 protein.展开更多
Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a ...Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a C-C chemokine receptor type 5 antagonist,has been viewed as a new therapeutic strategy for many neuroinflammatory diseases.We studied the effect of maraviroc on TBI-induced neuroinflammation.A moderate-TBI mouse model was subjected to a controlled cortical impact device.Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days.Western blot,immunohistochemistry,and TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling)analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI.Our results suggest that maraviroc administration reduced NACHT,LRR,and PYD domains-containing protein 3 inflammasome activation,modulated microglial polarization from M1 to M2,decreased neutrophil and macrophage infiltration,and inhibited the release of inflammatory factors after TBI.Moreover,maraviroc treatment decreased the activation of neurotoxic reactive astrocytes,which,in turn,exacerbated neuronal cell death.Additionally,we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score,rotarod test,Morris water maze test,and lesion volume measurements.In summary,our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI,and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.展开更多
CTX-M-producing bacteria are known as a resistant source against oxyimino-cephalosporin such as cefotaxime and ceftazidime;although laboratory diagnosis of this gene has not been properly defined. The aims of this stu...CTX-M-producing bacteria are known as a resistant source against oxyimino-cephalosporin such as cefotaxime and ceftazidime;although laboratory diagnosis of this gene has not been properly defined. The aims of this study are determining the rates of prevalence of CTX-M and CTX-M group 1 in the Escherichia coli (E. coli) obtained from urinary tract infections (UTI), and also determining their genetic relationship in the city of Sanandaj. In current study, 180 E. coli strains isolated from urinary tract infections were used. Sensitivity to common antibiotics was studied by the disc diffusion method. Phenotypic detection of isolated ESBL-producing starins was done by the combination disc test. CTX-M and CTX-M1 genes were detected using the PCR method and finally, the possible clonal relationship between isolates was determined using the REP-PCR method. 89 samples were ESBL-positive. The PCR assay used for detecting the CTX-M gene, showed that 48 samples out of 180 samples (26.66%) contained that gene;also among these 48 samples, 23 (12.77%) had CTX-M group 1. Based on the REP-PCR assay, 48 genotypes among 48 samples were CTX-M-positive. Results from the REP-PCR assay indicated that the clonal propagation theory of one epidemic strain of Escherichia coli is not apply, i.e. all CTX-M-producing species are not originated from one single strain and the gene is spread between different isolates. Therefore, hospitals and their employees must be more hygiene and, proper disposal of hospital waste can help to prevent the spread of different resistances.展开更多
To enlarge the middle-income group and construct the "olivary" income distribution becomes one of the important issues of the economic development and income distribution reform in China. The income distribu...To enlarge the middle-income group and construct the "olivary" income distribution becomes one of the important issues of the economic development and income distribution reform in China. The income distribution function is estimated with kernel density, and the income distribution M-curve is constructed with CHNS and CHIP data to calculate the middle-income group. Furthermore, a comparative analysis is carried out for the changing trend of the size and proportion of middle-income group. Research conclusion: it is discovered according to the income distribution M-curve that the key to the enlargement of urban middle-income group lies in the lower middle-income group, while the key to the enlargement of rural middle-income group lies in the improvement of the upper middle-income group. The range of middle-income group is expanding, but due to the small scale, low proportion, and poor stability, it has not developed the "olivary" income distribution structure yet, and income inequality tends to be deepened.展开更多
文摘Background: Extended-spectrum beta-lactamase (ESBL) producing bacteria are a real public health problem, particularly in Africa. Among these ESBLs, there are the Muenchen Cefotaximase (CTX-M) described all over the world of which the most frequent is the CTX-M of group 1 particularly the CTX-M-15 variant. The objective of this study was to determine the prevalence of CTX-M group 1 ESBL-producing Escherichia coli strains and to test their antibiotics susceptibility profile. Methodology: A retrospective cross-sectional descriptive study was conducted to detect ESBL-secreting Escherichia coli strains by the synergy test. Identification of CTX-M type ESBL from group 1 was performed using the NG-Test CTX-M rapid diagnostic test (NG-Biotech®). Antibiotic susceptibility profile was determined using CA-SFM/EUCAST guidelines 2019. Data entry and statistical analysis were performed with Excel version 2010 and SPSS 20.0 respectively. Results: Eighty-two ESBL-producing Escherichia coli strains were tested. A group 1 CTX-M ESBL was detected in 75.6% of the strains (n = 62). These strains were highly resistant to cefotaxim (100%), aztreonam (100%), ceftazidim (85.4%) and cefepim (66.1%). They were also resistant to quinolones, gentamycin and sulfadoxine-trimethoprim combination. However, these strains showed sensitivity to ertapenem (100%), cefoxitin (69.3%), tigecyclin (66%), and amikacin (66.1%). The combination of piperacillin and tazobactam was active on 30.6% of the strains against 6.4% for the combination of amoxicillin and clavulanic acid. Conclusion: The CTX-M type ESBL of group 1 was present in the majority of ESBL-producing Escherichia colis trains. Despite the production of this enzyme conferring resistance to most beta-lactam antibiotics, some antibiotics remain active to treat infections caused by these germs.
文摘Let S\-n be the symmetric group, g\++\-i=(123i),g\+-\-i=(1i32) and M\++\-n={g\++\-i∶4≤i≤n}, then M\++\-n is a minimal generating set of S\-n ,where n ≥5.It is proved that Cayley graph Cay( S\-n,M\++\-n∪M\+-\-n) is Hamiltonian and edge symmetric.
文摘Analytic atlases on <img src="Edit_948e45b7-cbef-425e-bb79-28648b859994.png" width="23" height="22" alt="" /> can be easily defined making it an <em>n</em>-dimensional complex manifold. Then with the help of bi-M<span style="white-space:nowrap;"><span style="white-space:nowrap;">ö</span></span>bius transformations in complex coordinates Abelian groups are constructed making this manifold a Lie group. Actions of Lie groups on differentiable manifolds are well known and serve different purposes. We have introduced in previous works actions of Lie groups on non orientable Klein surfaces. The purpose of this work is to extend those studies to non orientable <em>n</em>-dimensional complex manifolds. Such manifolds are obtained by factorizing <img src="Edit_7e5721ee-bb7f-4224-bd52-7d4641ac1c73.png" width="23" height="22" alt="" /> with the two elements group of a fixed point free antianalytic involution of <img src="Edit_ddfdac64-b296-48c5-9bb2-932eb3d76008.png" width="23" height="22" alt="" />. Involutions <strong>h(z)</strong> of this kind are obtained linearly by composing special M<span style="white-space:nowrap;"><span style="white-space:nowrap;">ö</span></span>bius transformations of the planes with the mapping <img src="Edit_4cda269a-9399-41ae-a5da-0c9d18a419ab.png" width="89" height="24" alt="" /><img src="Edit_4cda269a-9399-41ae-a5da-0c9d18a419ab.png" width="85" height="22" alt="" />. A convenient partition of <img src="Edit_9e899708-41b0-4351-a12b-cc6efb5b1581.png" width="23" height="22" alt="" /> is performed which helps defining an internal operation on <img src="Edit_7cd42987-68f8-4e4c-9382-cbc68b56377e.png" width="49" height="26" alt="" /> and finally actions of the previously defined Lie groups on the non orientable manifold <img src="Edit_5740b48c-f9ea-438d-a87d-8cdc1f83662b.png" width="49" height="25" alt="" /> are displayed.
基金suppor ted by the National Key Research and Development Program of China(2020YFA0908000)the Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine(ZYYCXTD-C-202002)+1 种基金the National Natural Science Foundation of China(82074098,81841001)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZXKT18003)。
文摘Background: Sepsis involves life-threatening organ dysfunction and is caused by a dysregulated host response to infection. No specific therapies against sepsis have been reported. Celastrol(Cel) is a natural anti-inflammatory compound that shows potential against systemic inflammatory diseases. This study aimed to investigate the pharmacological activity and molecular mechanism of Cel in models of endotoxemia and sepsis.Methods: We evaluated the anti-inflammatory efficacy of Cel against endotoxemia and sepsis in mice and macrophage cultures treated with lipopolysaccharide(LPS). We screened for potential protein targets of Cel using activity-based protein profiling(ABPP). Potential targets were validated using biophysical methods such as cellular thermal shift assays(CETSA) and surface plasmon resonance(SPR). Residues involved in Cel binding to target proteins were identified through point mutagenesis, and the functional effects of such binding were explored through gene knockdown.Results: Cel protected mice from lethal endotoxemia and improved their survival with sepsis, and it significantly decreased the levels of pro-inflammatory cytokines in mice and macrophages treated with LPS(P <0.05). Cel bound to Cys424 of pyruvate kinase M2(PKM2), inhibiting the enzyme and thereby suppressing aerobic glycolysis(Warburg effect). Cel also bound to Cys106 in high mobility group box 1(HMGB1) protein, reducing the secretion of inflammatory cytokine interleukin(IL)-1β. Cel bound to the Cys residues in lactate dehydrogenase A(LDHA).Conclusions: Cel inhibits inflammation and the Warburg effect in sepsis via targeting PKM2 and HMGB1 protein.
基金supported by grants from the National Natural Science Foundation of China, Nos. 81930031 (to JNZ), 81720108015 (to JNZ), 81901525 (to SZ), 82101440 (to DDS), 81801234 (to YZ) and 82071389 (to GLY)the Natural Science Foundation of Tianjin, Nos. 20JCQNJC01270 (to JWW), 20JCQNJC00460 (to GLY), 18JCQNJC81000 (to HTR)+4 种基金Scientific Research Project of Tianjin Education Commission (Natural Science), No. 2018KJ052 (to ZWZ)Tianjin Health and Health Committee Science and Technology Project, No. QN20015 (to JWW)the Science & Technology Development Fund of Tianjin Education Commission for Higher Education, No. 2016YD02 (to YW)Tianjin Key Science and Technology Projects of Innovative Drugs and Medical Devices, No. 19ZXYXSY00070 (to YW)the Clinical Research Fundation of Tianjin Medical University, No. 2018kylc002 (to YW)
文摘Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a C-C chemokine receptor type 5 antagonist,has been viewed as a new therapeutic strategy for many neuroinflammatory diseases.We studied the effect of maraviroc on TBI-induced neuroinflammation.A moderate-TBI mouse model was subjected to a controlled cortical impact device.Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days.Western blot,immunohistochemistry,and TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling)analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI.Our results suggest that maraviroc administration reduced NACHT,LRR,and PYD domains-containing protein 3 inflammasome activation,modulated microglial polarization from M1 to M2,decreased neutrophil and macrophage infiltration,and inhibited the release of inflammatory factors after TBI.Moreover,maraviroc treatment decreased the activation of neurotoxic reactive astrocytes,which,in turn,exacerbated neuronal cell death.Additionally,we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score,rotarod test,Morris water maze test,and lesion volume measurements.In summary,our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI,and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.
文摘CTX-M-producing bacteria are known as a resistant source against oxyimino-cephalosporin such as cefotaxime and ceftazidime;although laboratory diagnosis of this gene has not been properly defined. The aims of this study are determining the rates of prevalence of CTX-M and CTX-M group 1 in the Escherichia coli (E. coli) obtained from urinary tract infections (UTI), and also determining their genetic relationship in the city of Sanandaj. In current study, 180 E. coli strains isolated from urinary tract infections were used. Sensitivity to common antibiotics was studied by the disc diffusion method. Phenotypic detection of isolated ESBL-producing starins was done by the combination disc test. CTX-M and CTX-M1 genes were detected using the PCR method and finally, the possible clonal relationship between isolates was determined using the REP-PCR method. 89 samples were ESBL-positive. The PCR assay used for detecting the CTX-M gene, showed that 48 samples out of 180 samples (26.66%) contained that gene;also among these 48 samples, 23 (12.77%) had CTX-M group 1. Based on the REP-PCR assay, 48 genotypes among 48 samples were CTX-M-positive. Results from the REP-PCR assay indicated that the clonal propagation theory of one epidemic strain of Escherichia coli is not apply, i.e. all CTX-M-producing species are not originated from one single strain and the gene is spread between different isolates. Therefore, hospitals and their employees must be more hygiene and, proper disposal of hospital waste can help to prevent the spread of different resistances.
文摘To enlarge the middle-income group and construct the "olivary" income distribution becomes one of the important issues of the economic development and income distribution reform in China. The income distribution function is estimated with kernel density, and the income distribution M-curve is constructed with CHNS and CHIP data to calculate the middle-income group. Furthermore, a comparative analysis is carried out for the changing trend of the size and proportion of middle-income group. Research conclusion: it is discovered according to the income distribution M-curve that the key to the enlargement of urban middle-income group lies in the lower middle-income group, while the key to the enlargement of rural middle-income group lies in the improvement of the upper middle-income group. The range of middle-income group is expanding, but due to the small scale, low proportion, and poor stability, it has not developed the "olivary" income distribution structure yet, and income inequality tends to be deepened.
