Myricaria laxiflora,which grows along the Yangtze River in China,holds ornamental,ecological,and medicinal value.However,its wild population is threatened and currently designated protected as a national priority.The ...Myricaria laxiflora,which grows along the Yangtze River in China,holds ornamental,ecological,and medicinal value.However,its wild population is threatened and currently designated protected as a national priority.The present research was the first to sequence and assemble M.laxiflora’s mitochondrial genome and examine its structural characteristics and phylogenetic relationships with other sequenced Caryophyllales species.The mitochondrial double-stranded closed-ring genome of M.laxiflora was found to be 389,949 bp in length,containing numerous repetitive sequences and RNA editing sites,with 34 protein encoding,21 tRNA,and 3 rRNA genes.Although there are 22 fragments in the mitochondrial genome of M.laxiflora that are homologous to its chloroplast genome,they are incomplete gene fragments.Phylogenetic analysis demonstrated evolutionary associations with related populations and was in agreement with findings on the chloroplast genome.These findings not only lay a foundation for its preservation but also offer valuable insights for evolutionary analysis and plant breeding research.展开更多
目的探讨甲基转移酶5(methyltransferase-like 5,METTL5)在三阴乳腺癌(triple-negative breast cancer,TNBC)中的作用和潜在机制。方法采用免疫组织化学方法和Western blot检测TNBC肿瘤组织和细胞系中METTL5的表达情况。用靶向METTL5的s...目的探讨甲基转移酶5(methyltransferase-like 5,METTL5)在三阴乳腺癌(triple-negative breast cancer,TNBC)中的作用和潜在机制。方法采用免疫组织化学方法和Western blot检测TNBC肿瘤组织和细胞系中METTL5的表达情况。用靶向METTL5的shRNA(shRNA-METTL5)转染TNBC细胞后,用CCK-8、集落形成、伤口愈合以及Transwell实验分别检测细胞增殖活性、迁移与侵袭,Western blot检测Wnt/β-catenin信号关键蛋白的表达。构建异种移植瘤模型,验证敲降METTL5对TNBC细胞在体内生长以及Wnt/β-catenin信号活性的影响。结果METTL5在TNBC肿瘤组织和细胞系中表达上调(P<0.01)。敲降METTL5可抑制TNBC细胞的增殖、迁移和侵袭并降低了Wnt/β-catenin信号分子β-catenin、细胞周期蛋白(Cyclin)D1、基质金属蛋白酶(MMP)-2和MMP-7的表达(均P<0.01)。体内实验显示,敲降METTL5减缓了移植瘤生长和Wnt/β-catenin信号活性。结论敲降METTL5能抑制TNBC细胞的增殖、迁移与侵袭,其作用可能与抑制Wnt/β-catenin信号通路有关。展开更多
基金funded by the Youth Project of the Science and Technology Research Program of the Chongqing Education Commission(No.KJQN202303329)supported by the Scientific Research Project of Promoting Forestry Development through Science and Technology in Chongqing(No.YLKYQN2024-3).
文摘Myricaria laxiflora,which grows along the Yangtze River in China,holds ornamental,ecological,and medicinal value.However,its wild population is threatened and currently designated protected as a national priority.The present research was the first to sequence and assemble M.laxiflora’s mitochondrial genome and examine its structural characteristics and phylogenetic relationships with other sequenced Caryophyllales species.The mitochondrial double-stranded closed-ring genome of M.laxiflora was found to be 389,949 bp in length,containing numerous repetitive sequences and RNA editing sites,with 34 protein encoding,21 tRNA,and 3 rRNA genes.Although there are 22 fragments in the mitochondrial genome of M.laxiflora that are homologous to its chloroplast genome,they are incomplete gene fragments.Phylogenetic analysis demonstrated evolutionary associations with related populations and was in agreement with findings on the chloroplast genome.These findings not only lay a foundation for its preservation but also offer valuable insights for evolutionary analysis and plant breeding research.
