Objective:To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV)IgM,IgG and other antibody indicators in mice.Methods:A total of 90 mice with HCV were...Objective:To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV)IgM,IgG and other antibody indicators in mice.Methods:A total of 90 mice with HCV were selected as the research objects.All the mice were tested for HCV IgM and IgA antibody,and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay,magnetic particle chemiluminescence method and indirect agglutination method,and the results and positive rates of the three groups were compared.Results:There was no significant difference in the positive rate among the three groups(P>0.05).The specificity,accuracy and sensitivity of indirect agglutination test for IgM were 90.79%,80.00%and 30.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%,93.33%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05).For IgA,the specificity,accuracy and sensitivity of indirect agglutination assay were 90.67%,78.66%and 20.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%,92.35%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay,and the difference was statistically significant(P<0.05).Conclusion:The magnetic particle chemiluminescence method is more effective in the detection of IgM,IgG and other antibodies in mice with HCV.展开更多
成员判定是疏散星团研究中最关键的一步,成员判定的好坏直接影响对星团基本参数的估计.首次利用数据挖掘技术中的DBSCAN(Density-Based Spatial Clustering of Applications with Noise)聚类算法对疏散星团NGC 6791和M67(NGC 2682)分别...成员判定是疏散星团研究中最关键的一步,成员判定的好坏直接影响对星团基本参数的估计.首次利用数据挖掘技术中的DBSCAN(Density-Based Spatial Clustering of Applications with Noise)聚类算法对疏散星团NGC 6791和M67(NGC 2682)分别进行了成员判定,结果表明DBSCAN聚类算法能很有效地剔除场星污染.得到的NGC6791成员星的颜色-星等图上主序清晰并呈现明显的双重主序结构,这表明NGC 6791可能有更复杂的恒星形成与演化历史.对M67的分析表明出现了质量分层现象,并且星团的核心和外围两部分有明显的相对运动.对NGC 6791和M67的分析均表明DBSCAN聚类算法是一种有效的成员判定方法,有传统成员判定方法不具备的一些优点.展开更多
文摘Objective:To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV)IgM,IgG and other antibody indicators in mice.Methods:A total of 90 mice with HCV were selected as the research objects.All the mice were tested for HCV IgM and IgA antibody,and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay,magnetic particle chemiluminescence method and indirect agglutination method,and the results and positive rates of the three groups were compared.Results:There was no significant difference in the positive rate among the three groups(P>0.05).The specificity,accuracy and sensitivity of indirect agglutination test for IgM were 90.79%,80.00%and 30.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44%,93.33%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P<0.05).For IgA,the specificity,accuracy and sensitivity of indirect agglutination assay were 90.67%,78.66%and 20.00%,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25%,92.35%and80.00%,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay,and the difference was statistically significant(P<0.05).Conclusion:The magnetic particle chemiluminescence method is more effective in the detection of IgM,IgG and other antibodies in mice with HCV.
