本研究探讨应用荧光染色试剂Calcofluor White M2R染色鉴别家蚕微孢子虫Nosema bombycis。结果表明:在荧光显微镜下可见家蚕微孢子虫孢子被染上强烈的青蓝色荧光,而寄主组织碎片、病毒、细菌等不被染色。该法是一种快速有效鉴别微孢子...本研究探讨应用荧光染色试剂Calcofluor White M2R染色鉴别家蚕微孢子虫Nosema bombycis。结果表明:在荧光显微镜下可见家蚕微孢子虫孢子被染上强烈的青蓝色荧光,而寄主组织碎片、病毒、细菌等不被染色。该法是一种快速有效鉴别微孢子虫的方法。展开更多
采用蝗虫翅膀作为侵染组织,探讨了荧光染色剂Calcofluor White M2R在观测寄主体表绿僵菌孢子及其附着孢形成中的应用。结果表明,在荧光显微镜下,清晰可见蝗虫翅膀上发蓝色荧光的绿僵菌孢子、芽管及附着孢,而蝗虫翅膀未被染色,避免了干...采用蝗虫翅膀作为侵染组织,探讨了荧光染色剂Calcofluor White M2R在观测寄主体表绿僵菌孢子及其附着孢形成中的应用。结果表明,在荧光显微镜下,清晰可见蝗虫翅膀上发蓝色荧光的绿僵菌孢子、芽管及附着孢,而蝗虫翅膀未被染色,避免了干扰观察目标物。该方法可以准确观察病原真菌孢子在昆虫体表组织的萌发及附着孢形成。展开更多
[ Objective ] The aim of this study was to investigate the infectivity of Nosema bombycis to drosophila, which offered a new vision for systematical studies on the infection mechanism of Nosema bombycis, and also prov...[ Objective ] The aim of this study was to investigate the infectivity of Nosema bombycis to drosophila, which offered a new vision for systematical studies on the infection mechanism of Nosema bombycis, and also provided reference for the bio-control effect of Nosema bombycis. [ Method ] Nosema bombycis was used to feed wild type and mutant drosophila, and the morphological observation of Nosema bombycis in drosophila body fluid was also analyzed by calcofluor white M2R fluorescent staining. [ Result] Nosema bombycis could infect drosophila, and the number of Nosema bombycis in the infected mutant drosophila was higher than that in wild type drosophila. [ Conclusion ] Nosema bombycis can infect drosophila, which provides primary reference for studies on the infectivity of Nosema bombycis to other hosts and also lays a foundation for further study on the infection mechanism of Nosema bombycis.展开更多
[目的]研究家蚕微孢子虫对果蝇的侵染性,为系统研究家蚕微孢子虫侵染机制提供新视野,也为探索微孢子虫的生物防治效果提供参考。[方法]用家蚕微孢子添食野生型和突变型果蝇,并用Calcofluor White M2R荧光染色法进行果蝇体液内的家蚕微...[目的]研究家蚕微孢子虫对果蝇的侵染性,为系统研究家蚕微孢子虫侵染机制提供新视野,也为探索微孢子虫的生物防治效果提供参考。[方法]用家蚕微孢子添食野生型和突变型果蝇,并用Calcofluor White M2R荧光染色法进行果蝇体液内的家蚕微孢子虫形态观察。[结果]结果表明,家蚕微孢子虫在转宿主情况下能够侵染果蝇,且被感染的突变型果蝇体内家蚕微孢子虫的数量多于野生型果蝇。[结论]家蚕微孢子虫能侵染果蝇,该研究结果为研究家蚕微孢子虫侵染其他宿主提供了初步参考,为进一步了解和认识家蚕微孢子虫侵染机制奠定了基础。展开更多
目的荟萃分析β肾上腺素能受体自身抗体(β-AR自身抗体)、M_2毒蕈碱受体自身抗体(M_2R自身抗体)与"特发性"心律失常之间的关系。方法通过检索CNKI中国期刊全文数据库、万方数据库、Pubmed数据库、ISI Web of Science数据库搜...目的荟萃分析β肾上腺素能受体自身抗体(β-AR自身抗体)、M_2毒蕈碱受体自身抗体(M_2R自身抗体)与"特发性"心律失常之间的关系。方法通过检索CNKI中国期刊全文数据库、万方数据库、Pubmed数据库、ISI Web of Science数据库搜索研究,不限制语言和年份(建库至2017年11月),并根据纳入标准进行筛选。2名研究者独立从所纳入研究中提取数据。质量评估采用修改后的纽卡斯尔-渥太华量表(NOS)进行。采用Stata.12软件进行统计学分析。采用漏斗图检测其发表偏倚。结果共纳入12个研究。累积病例608例,对照组534例,荟萃分析结果显示:"特发性"心律失常患者病例组与对照组相比,β-AR自身抗体、M_2R自身抗体水平更高。10篇文章质量评分均≥5分,为较高质量文献。6篇报告了β-AR自身抗体阳性率,合并显示,异质性检验I_2=56.4%,P=0.043,P<0.05,存在轻度异质性,用随机效应模型,合并的OR有统计学意义(OR=8.24,95%CI:3.83~17.72,Z=5.4,P=0.00)。2篇文献通过计算可获得β-AR自身抗体水平的均数标准差,进行合并,结果显示合并的SMD有统计学意义(SMD=0.82,95%CI:0.36~1.29,Z=3.48,P=0.001)。3篇文献通过计算可获得M_2R自身抗体水平均数标准差,合并显示,异质性检验I_2=63.6%,P=0.064,不存在异质性,用随机效应模型,合并的SMD有统计学意义(SMD=0.95,95%CI:0.57~1.33,Z=4.89,P=0.000)。结论 "特发性"心律失常患者中β-AR自身抗体、M_2R自身抗体检出率更高。展开更多
为提高木霉几丁质酶检测方法的准确性和灵敏度,建立一种快速检测几丁质酶同工酶的方法。采用活性凝胶电泳、变性凝胶电泳、原位显色凝胶电泳结合荧光增白剂(Calcofluor white M2R)显色从绿色木霉LTR-2发酵产物中检测几丁质酶同工酶。活...为提高木霉几丁质酶检测方法的准确性和灵敏度,建立一种快速检测几丁质酶同工酶的方法。采用活性凝胶电泳、变性凝胶电泳、原位显色凝胶电泳结合荧光增白剂(Calcofluor white M2R)显色从绿色木霉LTR-2发酵产物中检测几丁质酶同工酶。活性凝胶电泳在粗酶液浓缩5倍时显示两条活性谱带,变性凝胶电泳在浓缩10倍时显示一条活性谱带,原位显色凝胶电泳在浓缩20倍时显示两条不清晰的活性谱带,SDS-PAGE显示这两条活性谱带的分子量分别为65kDa和42kDa。结果表明活性聚丙烯酰胺凝胶电泳和Calcofluor white M2R显色相结合的方法在几丁质酶上样量为0.47U时具有较好的分辨能力,是检测木霉几丁质酶同工酶的有效的方法。展开更多
The stability of the Schwarzschild black hole is restudied in the Painlevé coordinates. Using the Painlevé time coordinate to define the initial time, we reconsider the odd perturbation and find that the Sch...The stability of the Schwarzschild black hole is restudied in the Painlevé coordinates. Using the Painlevé time coordinate to define the initial time, we reconsider the odd perturbation and find that the Schwarzschild black hole in the Painlevé coordinates is unstable. The Painlevé metric in this paper corresponds to the white-hole-connected region of the Schwarzschild black hole （r 〉 2m） and the odd perturbation may be regarded as the angular perturbation. Therefore, the white-hole-connected region of the Schwarzschild black hole is unstable with respect to the rotating perturbation.展开更多
文摘采用蝗虫翅膀作为侵染组织,探讨了荧光染色剂Calcofluor White M2R在观测寄主体表绿僵菌孢子及其附着孢形成中的应用。结果表明,在荧光显微镜下,清晰可见蝗虫翅膀上发蓝色荧光的绿僵菌孢子、芽管及附着孢,而蝗虫翅膀未被染色,避免了干扰观察目标物。该方法可以准确观察病原真菌孢子在昆虫体表组织的萌发及附着孢形成。
基金Supported by Natural Science Foundation of Chongqing(2008BB1368)~~
文摘[ Objective ] The aim of this study was to investigate the infectivity of Nosema bombycis to drosophila, which offered a new vision for systematical studies on the infection mechanism of Nosema bombycis, and also provided reference for the bio-control effect of Nosema bombycis. [ Method ] Nosema bombycis was used to feed wild type and mutant drosophila, and the morphological observation of Nosema bombycis in drosophila body fluid was also analyzed by calcofluor white M2R fluorescent staining. [ Result] Nosema bombycis could infect drosophila, and the number of Nosema bombycis in the infected mutant drosophila was higher than that in wild type drosophila. [ Conclusion ] Nosema bombycis can infect drosophila, which provides primary reference for studies on the infectivity of Nosema bombycis to other hosts and also lays a foundation for further study on the infection mechanism of Nosema bombycis.
文摘[目的]研究家蚕微孢子虫对果蝇的侵染性,为系统研究家蚕微孢子虫侵染机制提供新视野,也为探索微孢子虫的生物防治效果提供参考。[方法]用家蚕微孢子添食野生型和突变型果蝇,并用Calcofluor White M2R荧光染色法进行果蝇体液内的家蚕微孢子虫形态观察。[结果]结果表明,家蚕微孢子虫在转宿主情况下能够侵染果蝇,且被感染的突变型果蝇体内家蚕微孢子虫的数量多于野生型果蝇。[结论]家蚕微孢子虫能侵染果蝇,该研究结果为研究家蚕微孢子虫侵染其他宿主提供了初步参考,为进一步了解和认识家蚕微孢子虫侵染机制奠定了基础。
文摘目的荟萃分析β肾上腺素能受体自身抗体(β-AR自身抗体)、M_2毒蕈碱受体自身抗体(M_2R自身抗体)与"特发性"心律失常之间的关系。方法通过检索CNKI中国期刊全文数据库、万方数据库、Pubmed数据库、ISI Web of Science数据库搜索研究,不限制语言和年份(建库至2017年11月),并根据纳入标准进行筛选。2名研究者独立从所纳入研究中提取数据。质量评估采用修改后的纽卡斯尔-渥太华量表(NOS)进行。采用Stata.12软件进行统计学分析。采用漏斗图检测其发表偏倚。结果共纳入12个研究。累积病例608例,对照组534例,荟萃分析结果显示:"特发性"心律失常患者病例组与对照组相比,β-AR自身抗体、M_2R自身抗体水平更高。10篇文章质量评分均≥5分,为较高质量文献。6篇报告了β-AR自身抗体阳性率,合并显示,异质性检验I_2=56.4%,P=0.043,P<0.05,存在轻度异质性,用随机效应模型,合并的OR有统计学意义(OR=8.24,95%CI:3.83~17.72,Z=5.4,P=0.00)。2篇文献通过计算可获得β-AR自身抗体水平的均数标准差,进行合并,结果显示合并的SMD有统计学意义(SMD=0.82,95%CI:0.36~1.29,Z=3.48,P=0.001)。3篇文献通过计算可获得M_2R自身抗体水平均数标准差,合并显示,异质性检验I_2=63.6%,P=0.064,不存在异质性,用随机效应模型,合并的SMD有统计学意义(SMD=0.95,95%CI:0.57~1.33,Z=4.89,P=0.000)。结论 "特发性"心律失常患者中β-AR自身抗体、M_2R自身抗体检出率更高。
文摘为提高木霉几丁质酶检测方法的准确性和灵敏度,建立一种快速检测几丁质酶同工酶的方法。采用活性凝胶电泳、变性凝胶电泳、原位显色凝胶电泳结合荧光增白剂(Calcofluor white M2R)显色从绿色木霉LTR-2发酵产物中检测几丁质酶同工酶。活性凝胶电泳在粗酶液浓缩5倍时显示两条活性谱带,变性凝胶电泳在浓缩10倍时显示一条活性谱带,原位显色凝胶电泳在浓缩20倍时显示两条不清晰的活性谱带,SDS-PAGE显示这两条活性谱带的分子量分别为65kDa和42kDa。结果表明活性聚丙烯酰胺凝胶电泳和Calcofluor white M2R显色相结合的方法在几丁质酶上样量为0.47U时具有较好的分辨能力,是检测木霉几丁质酶同工酶的有效的方法。
基金Project supported by the National Natural Science Foundation of China （Grant Nos 10475013, 10373003 and 10375087）, the National Basic Research Program （Grant No 2004CB318000） and the Post-Doctor Foundation of China.
文摘The stability of the Schwarzschild black hole is restudied in the Painlevé coordinates. Using the Painlevé time coordinate to define the initial time, we reconsider the odd perturbation and find that the Schwarzschild black hole in the Painlevé coordinates is unstable. The Painlevé metric in this paper corresponds to the white-hole-connected region of the Schwarzschild black hole （r 〉 2m） and the odd perturbation may be regarded as the angular perturbation. Therefore, the white-hole-connected region of the Schwarzschild black hole is unstable with respect to the rotating perturbation.
