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Molecular cloning,identification,and chromosomal localization of two MADS box genes in peach(Prunus persica) 被引量:13
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作者 Lin Zhang Yong Xu Rongcai Ma 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第6期365-372,共8页
MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADSIO, were cloned using degenerate prime... MADS box proteins play an important role in floral development. To find genes involved in the floral transition of Prunus species, cDNAs for two MADS box genes, PpMADS1 and PpMADSIO, were cloned using degenerate primers and 5'- and T-RACE based on the sequence database of P. persiea and P. duleis. The full length of PpMADS1 cDNA is 1,071 bp containing an open reading frame (ORF) of 717 bp and coding for a polypeptide of 238 amino acid residues. The full length of PpMADSIO cDNA is 937 bp containing an ORF of 633 bp and coding for a polypeptide of 210 amino acid residues. Sequence comparison revealed that PpMADS1 and PpMADSIO were highly homologous to genes API and PI in Arabidopsis, respectively. Phylogenetic analysis indicated that PpMADS1 belongs to the euAP1 clade of class A, and PpMADSIO is a member of GLO/PI clade of class B. RT-PCR analysis showed that PpMADS1 was expressed in sepal, petal, carpel, and fruit, which was slightly different from the expression pattern ofAPl; PpMADS10 was expressed in petal and stamen, which shared the same expression pattern as PI. Using selective mapping strategy, PpMADSI was assigned onto the Binl:50 on the G1 linkage group between the markers MCO44 and TSA2, and PpMADSIO onto the Bin1:73 on the same linkage group between the markers Lap- 1 and FGA8. Our results provided the basis for further dissection of the two MADS box gene function. 展开更多
关键词 peach (Prunus persica) flower development mads box gene selective mapping
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