This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans a...This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans and DNA d(T) 18 . The results demonstrated that α cyano ferulic acid used as a new matrix could effectively desorb and ionize different kinds of samples, such as proteins and peptides, oligosaccharides, polysaccharides and oligonucleotides. In analysis of dextrans the new matrix gave better results when comparing with the normally used DHB. In analysis of DNA d(T) 18 , the sample could be desorbed and ionized more efficiently even without sample purification, which is normally needed when using 3 HPA as a matrix.展开更多
基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF MS)是一种可以对多种微生物(包括细菌、酵母和丝状真菌)进行常规鉴定的强有力工具。具有快速、准确、高...基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry,MALDI-TOF MS)是一种可以对多种微生物(包括细菌、酵母和丝状真菌)进行常规鉴定的强有力工具。具有快速、准确、高通量及成本低廉等特点,逐渐被用于丝状真菌感染的实验室诊断中。在丝状真菌的MALDI-TOF MS分析中,从样本制备到获得准确的鉴定结果,每个步骤都决定了医技人员能否及时为临床医生提供准确结果。现就待测样品的制备及MALDI-TOF MS技术对丝状真菌分析(包括鉴定、分型、抗真菌敏感性测试方面)的应用及前景作一系统综述,也将对其一些未来发展进行展望。展开更多
文摘This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans and DNA d(T) 18 . The results demonstrated that α cyano ferulic acid used as a new matrix could effectively desorb and ionize different kinds of samples, such as proteins and peptides, oligosaccharides, polysaccharides and oligonucleotides. In analysis of dextrans the new matrix gave better results when comparing with the normally used DHB. In analysis of DNA d(T) 18 , the sample could be desorbed and ionized more efficiently even without sample purification, which is normally needed when using 3 HPA as a matrix.
