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MAP18参与ABA调控拟南芥花粉生长的研究 被引量:3
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作者 林坤 周生波 +1 位作者 王超 侍福梅 《安徽农业科学》 CAS 2012年第13期7629-7630,7734,共3页
[目的]探讨微管结合蛋白MAP18在ABA调控拟南芥花粉生长中的作用,为进一步深入研究植物的极性发育模式提供线索。[方法]以拟南芥野生型(生态型Columbia,Col-0)及相关突变体花粉为试验材料,进行遗传学和药理学分析,研究花粉萌发和花粉管... [目的]探讨微管结合蛋白MAP18在ABA调控拟南芥花粉生长中的作用,为进一步深入研究植物的极性发育模式提供线索。[方法]以拟南芥野生型(生态型Columbia,Col-0)及相关突变体花粉为试验材料,进行遗传学和药理学分析,研究花粉萌发和花粉管生长的调控因子。[结果]内源和外源ABA均显著抑制拟南芥花粉的萌发,但内源ABA对萌发的花粉管有促进伸长的作用。遗传学证据表明,微管结合蛋白MAP18过表达植株的花粉对ABA表现超敏感,而RNAi沉默株的花粉对ABA则表现出脱敏感现象,显示MAP18蛋白参与了ABA对花粉萌发及花粉管生长的调控过程。[结论]该试验结果为研究花粉萌发与花粉管生长调控模式提供了线索。 展开更多
关键词 拟南芥 花粉 突变体 脱落酸 微管结合蛋白map18
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MAP18参与了脱落酸调控的拟南芥气孔关闭及根生长 被引量:3
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作者 刘广志 陈炳佑 侍福梅 《江苏农业科学》 北大核心 2015年第11期55-57,共3页
以拟南芥野生型、GFP-α-tubulin-6转基因拟南芥及微管结合蛋白MAP18相关突变体为材料,利用药理学试验及激光扫描共聚焦显微技术研究了在脱落酸(ABA)诱导叶片气孔关闭过程中保卫细胞微管骨架的动态变化、根生长情况及其可能的调控因子... 以拟南芥野生型、GFP-α-tubulin-6转基因拟南芥及微管结合蛋白MAP18相关突变体为材料,利用药理学试验及激光扫描共聚焦显微技术研究了在脱落酸(ABA)诱导叶片气孔关闭过程中保卫细胞微管骨架的动态变化、根生长情况及其可能的调控因子。结果表明,微管结合蛋白MAP18过表达拟南芥OE的叶片气孔关闭现象敏感,MAP18 RNAi沉默株R18现象相对不敏感。外源ABA抑制拟南芥幼苗初生根的生长试验中,突变体OE根伸长减慢的现象显著,伸长速率由0.60 cm/d降低至0.12 cm/d,未处理时WT、map18的伸长速率都要低于OE的伸长速率,而用5μmol/L ABA处理之后,WT、map18的伸长速率高于OE的伸长速率。在此基础上研究了参与其中的作用因子,推测MAP18通过影响微管的动态重组参与到ABA诱导拟南芥气孔关闭和根伸长的生理过程。 展开更多
关键词 map18 拟南芥 脱落酸 气孔 幼根 微管结合蛋白
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Hot Deformation Behavior and Processing Maps of As-cast Mn18Cr18N Steel 被引量:3
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作者 陈慧琴 wang zhenxing +2 位作者 qin fengming jia peijie zhao xiaodong 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2017年第4期935-943,共9页
Hot deformation behavior of as-cast Mn18Cr18 N austenitic stainless steel was studied in the temperature range of 950-1200 ℃ and strain rate range of 0.001-1 s^(-1) using isothermal hot compression tests. The true ... Hot deformation behavior of as-cast Mn18Cr18 N austenitic stainless steel was studied in the temperature range of 950-1200 ℃ and strain rate range of 0.001-1 s^(-1) using isothermal hot compression tests. The true stress-strain curves of the steel were characterized by hardening and subsequent softening and varied with temperatures and strain rates. The hot deformation activation energy of the steel was calculated to be 657.4 k J/mol, which was higher than that of the corresponding wrought steel due to its as-cast coarse columnar grains and heterogeneous structure. Hot processing maps were developed at different plastic strains, which exhibited two domains with peak power dissipation efficiencies at 1150 ℃/0.001 s^(-1) and 1200 ℃/1 s^(-1), respectively. The corresponding microstructures were analyzed by optical microscopy(OM), scanning electron microscopy(SEM), and electron backscatter diffraction(EBSD). It has been confirmed that dynamic recrystallization(DRX) controlled by dislocation slipping and climbing mechanism occurs in the temperature and strain rate range of 1050-1200 ℃ and 0.001-0.01 s^(-1); And DRX controlled by twinning mechanism occurs in the temperature and strain rate range of 1100-1200 ℃, 0.1-1 s^(-1). These two DRX domains can serve as the hot working windows of the as-cast steel at lower strain rates and at higher strain rates, respectively. The processing maps at different strains also exhibit that the instability region decreases with increasing strain. The corresponding microstructures and the less tensile ductility in the instability region imply that the flow instability is attributed to flow localization accelerated by a few layers of very fine recrystallized grains along the original grain boundaries. 展开更多
关键词 Mn18Cr18N steel hot deformation hot processing map dynamic recrystallization hot workability
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