Background: The article outlines the ways of preparing and sowing various samples for research with a view to isolating mycobacteriophages (MBphages), as well as developing methods for their isolation. Methods: Experi...Background: The article outlines the ways of preparing and sowing various samples for research with a view to isolating mycobacteriophages (MBphages), as well as developing methods for their isolation. Methods: Experimental studies on the obtaining of MB phages active against tubercle mycobacteria were conducted using samples collected from environmental objects and biological material from the different areas of the country. As an indicator of test-cultures, we have used the following M. kansasii, M. avium, M. scrofulaseum, M. phlei, M. terrae, М. intracellulare, M. smegmatis. For the cultivation of Mycobacteria and MB phages were used the following nutrient media of the company HiMedia: Dubos Broth Base, Dubos Oleic Agar Base, Kirschner Medium Base-Modified, Middlebrook 7H10 Agar Base, Peizer TB Medium Base, Lowenstein Jensen medium base и Sauton’s Fluid Medium. Results: During the research, the following results were obtained: An effective method for obtaining MB phages from environmental objects was select-ed. Four types of Mbphage were obtained, lysing atypical mycobacteria from environmental objects delivered from various areas of the Republic of Ka-zakhstan. Conclusion: Obtained data in the development of the method of obtaining MBphage, allows us to consider it necessary to further study their biological features, as well as the possibility of using them to develop an an-ti-tuberculosis effective agent.展开更多
文摘Background: The article outlines the ways of preparing and sowing various samples for research with a view to isolating mycobacteriophages (MBphages), as well as developing methods for their isolation. Methods: Experimental studies on the obtaining of MB phages active against tubercle mycobacteria were conducted using samples collected from environmental objects and biological material from the different areas of the country. As an indicator of test-cultures, we have used the following M. kansasii, M. avium, M. scrofulaseum, M. phlei, M. terrae, М. intracellulare, M. smegmatis. For the cultivation of Mycobacteria and MB phages were used the following nutrient media of the company HiMedia: Dubos Broth Base, Dubos Oleic Agar Base, Kirschner Medium Base-Modified, Middlebrook 7H10 Agar Base, Peizer TB Medium Base, Lowenstein Jensen medium base и Sauton’s Fluid Medium. Results: During the research, the following results were obtained: An effective method for obtaining MB phages from environmental objects was select-ed. Four types of Mbphage were obtained, lysing atypical mycobacteria from environmental objects delivered from various areas of the Republic of Ka-zakhstan. Conclusion: Obtained data in the development of the method of obtaining MBphage, allows us to consider it necessary to further study their biological features, as well as the possibility of using them to develop an an-ti-tuberculosis effective agent.
