葛根素对糖尿病大鼠胰腺线粒体MDA含量、SOD和ATP酶活性的影响

目的观察葛根素对实验性糖尿病大鼠胰腺线粒体丙二醛(MDA)含量、超氧化物歧化酶(SOD)和ATP酶活性的影响。方法30只Wistar大鼠随机分为正常对照组、糖尿病组和治疗组。采用四氧嘧啶腹腔注射复制糖尿病动物模型。葛根素注射液治疗8w后,测定血清葡萄糖、胰岛素、胰腺线粒体MDA、SOD含量和Na+-K+-ATP酶、Ca2+-ATP酶活性。结果糖尿病组大鼠血糖和MDA含量明显高于正常组(P<0.001),而血清胰岛素、SOD、Na+-K+-ATP酶和Ca2+-ATP酶活性显著降低(P<0.001);使用葛根素治疗后,与糖尿病组比较,治疗组血糖及MDA含量降低(P<0.05～0.001),血清胰岛素、SOD、Na+-K+-ATP酶和Ca2+-ATP酶活性升高(P<0.05～0.01)。结论葛根素对糖尿病大鼠胰腺有保护作用。

养颜青娥丸治疗肾阳不足型黄褐斑的作用机制研究

目的:观察肾阳不足型黄褐斑患者的发病机制并验证养颜青娥丸的有效性及作用机理。方法:将肾阳不足型黄褐斑患者随机分成治疗组(养颜青娥丸组)和对照组(桂附地黄丸组),分别测定治疗前、后患者的血清超氧化物歧化酶(SOD)、丙二醛(MDA)酶活性及空腹性激素水平并进行比较分析。结果:患者治疗前血清SOD酶活性显著低于正常对照组,治疗后SOD酶活性明显增加;治疗前MDA酶活性,血清雌二醇(E2)显著高于正常对照组,治疗后MDA活性、血清E2水平明显下降。运用t检验将对照组与治疗组治疗前、后数据分别进行比较,血清SOD、MDA酶活性和血清E2之间均有显著差异(P<0.05,P<0.01),对照组与治疗组治疗后相比无显著差异,总有效率比较,经t检验,P<0.05。结论:养颜青娥丸能提高肾阳不足型黄褐斑患者血清的SOD酶活性并降低MDA酶的活性和血清E2的水平,从而降低酪氨酸酶活性,减少黑素的形成,使黑素的形成受到抑制而对黄褐斑产生疗效,且临床疗效优于对照组。

Research on Survival Conditions of Bellamya sp.in Different Aquatic Vegetation Habitats in Taihu Area

[Objective] The paper was to investigate the survival conditions of Bel- lamya sp. in different aquatic vegetation habitats in Taihu area, so as to provide suitable evaluation indexes for environmental monitoring in the area. [Method] Equal density of Bellamya sp. was placed in 3 aquatic vegetation habitats （emerged plant area, submerged plant area and non-plant area） in ecological engineering construc- tion area near the west of Wangyuhe in Taihu, the survival rate, SOD activity, MDA content and P450 enzyme activity of Bellamya sp. during outbreak and non-outbreak stage of cyanobacteria were determined, and the correlation between these indexes and ammonia nitrogen content of lake water was also analyzed. [Result] During the outbreak stage of cyanobacteria, the survival rates of Bellamya sp. in emerged plant area, submerged plant area and non-plant area were 0%, 55.2% and 89.3%, re- spectively, SOD activity first decreased and then increased, the change trend of MDA content was opposite with SOD change trend, P450 enzyme activity continu- ously decreased. During non-outbreak stage of cyanobacteria, the survival rates of Bellamya sp. in 3 habitats were 90.6% ,98.8 and 99.5%, respectively, SOD activity first decreased and then increased, MDA content first increased and then decreased, the changes of P450 enzyme activity were not regular. SOD activity of Bellamya sp. during 2 phases had significantly negative correlation with ammonia nitrogen content of lake water, MDA content had significantly positive correlation with ammonia nitro- gen content of lake water only during non-outbreak stage of cyanobacteria, P450 enzyme activity has no significant correlation with ammonia nitrogen content. This in- dicated that when Bellamya sp. was used to evaluate the impact of aquatic vegeta- tion on benthic animals, P450 enzyme activity was not the suitable indicator. [Conclusion] SOD activity in muscle tissue of Bellamya sp. could be used as the indica- tor for evaluation of habitat, MDA could be used as indicator for detection of am- monia nitrogen content during non-outbreak stage of cyanobacteria, P450 enzyme could not be used as the indicator for early diagnosis and early warning of environ- mental pollution in 3 habitats in Taihu area.

益气健脾祛斑汤治疗脾虚血瘀型黄褐斑61例

目的研究益气健脾祛斑汤治疗脾虚血瘀型黄褐斑的疗效及机理。方法将61例黄褐斑患者随机分为治疗组(益气健脾祛斑汤组)和对照组(人参健脾丸组)。治疗2个月后评价治疗效果。结果治疗前后比较,临床疗效显著提高,黄褐斑评分显著降低,有显著差异(XP<0.01),SOD酶有显著升高(※P<0.01),MDA酶明显降低(□P<0.05),治疗组优于对照组。结论益气健脾祛斑汤临床疗效好且无毒副作用,其机理是通过升高SOD酶,降低MDA酶水平达到祛斑作用的。

Chitosan Oligosaccharide-Ca Complex Accelerates the Depuration of Cadmium from Chlamys ferrari

This study investigated the effect of a chitosan oligosaccharide-Ca complex (COS-Ca) on the depuration of cadmium (Cd) from Chlamys ferrari. After exposure to 0.5 mg L-1 CdCl2 for 3 or 7 d, the scallops were treated by COS-Ca prior to determina-tion of Cd, calcium (Ca) and zinc (Zn) contents, Cd distribution in organs, malondialdehyde (MDA) content and antioxidant variables. Results showed that COS-Ca reduced Cd content in the viscera of the scallops, with highest Cd depuration rate (47%) observed on day 3. The COS-Ca concentration substantially affected Cd depuration, and the exposure to 8.75 mg L-1 COS-Ca led to significantly higher Cd depuration rate compared with those of lower COS-Ca concentrations (1.75, 3.5, 5.25, and 7.00 mg L-1). Distribution analysis of Cd in scallop organs indicated that COS-Ca significantly reduced Cd content in the kidney throughout the 5-d experiment, as well as in the gill during the early stage of Cd depuration. In addition, COS-Ca treatment decreased glutathione peroxidase (GSH-Px) activity and MDA content while increasing superoxide dismutase (SOD) and catalase (CAT) activities on different days. Our work suggested COS-Ca complex treatment as an effective method for acceleration of Cd depuration from Cd-contaminated bivalves.

Effects of benzo(a)pyrene exposure on the antioxidant enzyme activity of scallop Chlamys farreri

Scallop Chlamys farreri was exposed to different concentrations of benzo(a)pyrene (BaP) (0.5 μg/L, 1.0 μg/L, 10.0 μg/L and 50.0 μg/L) for 30 days in seawater. The 7-ethoxyresorufin O-deethylase (EROD) activity was significantly induced, and increased with the increasing BaP concentration. The glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx) activities increased in short time at low concentration of BaP, and was significantly depressed at high concentrations. Scallop gill was more sensitive to BaP than the digestive gland, and the digestive gland was the main tissue to deal with oxyradicals. The contents of malondialdehyde (MDA) increased with the exposure time and there was a positive correlation (concentration-effect) between the MDA content and the concentration of BaP. The biomarkers determined in this experiment had important roles in detoxification, and showed great potential as biomarkers for oxidative stress. Controlled laboratory experiments designed to simulate field exposure scenarios are particularly useful in ascertaining biomarkers suitable for use with complex contaminant mixtures in the marine environment.

Physiological characteristics changes of Aesculus chinensis seeds during natural dehydration

A study was conducted to determine the physiological characteristics changes of Aesculus chinensis seeds during natural dehydration in 2003. The results showed that A. chinensis seeds were recalcitrant with being highly desiccation-sensitive. The seed moisture content of fresh fruits was higher than 60%. When the seeds were naturally dried for 30 days, their moisture content declined to 30.2% and their viability was completely lost. The seed germination percentage had a small increase at the beginning of desiccation and then decreased rapidly. The relative electrical conductivity of the A. chinensis seeds increased along with a decrease in seed moisture content. However, there was an abnormal increase in relative electrical conductivity when the seed moisture content was between 53.7% and 50.9%. Superoxide dismutase （SOD） activity decreased rapidly in the period of desiccation except for an abnormality when the seed moisture content was between 53.7% and 50.9%. Malondialdehyde （MDA） content increased slowly at the early stage of desiccation and then rose rapidly after the moisture content was below 50.9%. The soluble sugar content in seeds slowly increased with the increasing period of desiccation. The seed germination percentage was at the high level when seed moisture content was in range of 47%- 60%, which suggests that this was the optimum moisture content for maintaining A. chinensis seed viability.

Toxic Dinoflagellate Alexandrium tamarense Induces Oxidative Stress and Apoptosis in Hepatopancreas of Shrimp(Fenneropenaeus chinensis)

This study investigated the inductive effect ofAlexandrium tamarense, a toxic dinoflagellate producing paralytic shell- fish poison, on oxidative stress and apoptosis in hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The individuals of E chinensis were exposed to 200 and 1000 cells mL-1 of A. tamarense with their superoxide dismutase （SOD）, glutathione S-transferase （GST） activities, malonyldialdehyde （MDA） concentration, and caspase gene （FcCasp） expression in hepatopancreas determined at 12, 24, 48, 72 and 96 h. In addition, apoptosis in hepatopancreas of E chinensis at 96 h after exposure was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay. The hepatopancreatic SOD and GST activities of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense showed a bell-shaped response to exposure time. The hepatopancreatic MDA concentration ofF. chinensis exposed to 1000 cellsmL-1 ofA. tamarense increased gradually from 48 to 96h, and such a trend corresponded to the decrease of GST activity. The hepatopancreatic FcCasp transcript abundance of F. chinensis exposed to 1000 cells mL-1 ofA. tamarense was positively and linearly correlated to MDA concentration. Results of TUNEL assay showed that exposure to 1000 cells mL-1 of A. tamarense induced apoptosis in the hepatopancreas of E chinensis. Our study revealed that A. tamarense exposure influenced the antioxidative status ofF. chinensis and caused lipid peroxidation and apoptosis in the hepatopancreas of shrimp.

Variations of antioxidant enzyme activity and malondialdehyde content in nemertean Cephalothrix hongkongiensis after exposure to heavy metals

The antioxidant enzyme activity and malondialdehyde(MDA) content of Cephalothrix hongkongiensis were studied to assess variations in the biochemical/physiological parameters of nemerteans under heavy metal stress.Worms were exposed to copper,zinc and cadmium solutions at different concentrations,and the activity of three antioxidant enzymes,catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GPX),and MDA content were measured.The results show that the activity of each enzyme changed immediately after exposure to heavy metals.CAT was invariably inhibited throughout the experimental period,while the SOD activity was significantly elevated by exposure to Cu^(2+) for 48h,but then decreased.SOD was inhibited by Zn^(2+) during the first 12h of exposure,but activated when exposed for longer periods.Under Cd^(2+) stress,SOD activity decreased within 72h.GPX activity varied greatly,being significantly increased by both Cu^(2+) and Zn^(2+),but significantly inhibited by Cd^(2+) in the first 12-24h after exposure.MDA content increased on Cu^(2+) exposure,but normally decreased on Zn^(2+) exposure.MDA content followed an increase-decrease-increase pattern under Cd^(2+) stress.In conclusion,the antioxidant system of this nemertean is sensitive to heavy metals,and its CAT activity may be a potential biomarker for monitoring heavy metal levels in the environment.

Effects of Boscalid on the Antioxidant Enzyme System of Adult Zebrafish (Danio rerio)

Abstract [Objective] This study was conducted to evaluate the ecotoxicity of boscalid to adult zebrafish （Danio rerio）. [Method] The activities of antioxidant enzymes superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, glutathione peroxidase （GPx）, as well as a non-enzymatic antioxidant malondialdehyde （MDA）, in the liver were measured 3, 7, 14 and 21 d post exposure （dpe） to 0.02 （1/100 of acute toxicity）, 0.036 （monitored concentration）, 0.08 （1/20 of acute toxicity）, 0.16 （1/10 of acute toxicity） and 0.32 mg/L （1/5 of acute toxicity） boscalid using a semi-static method. [Result] SOD, CAT, POD, GPx and MDA activity in the liver of zebrafish varied with boscalid concentration and exposure time. Boscalid significantly enhanced MDA content at 21 dpe. A significant upregulation of the activity of SOD, CAT, POD and GPx at 7 dpe was observed, suggesting that boscalid resulted in oxidative stress and lipid peroxidation. [Conclusion] These results show that these biomarkers are all appropriate for monitoring oxidative stress and the lipid peroxidation status of fish after exposure to boscalid. Key words Boscalid; Zebrafish; Antioxidant enzyme