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Extreme dependencies and spillovers between gold and stock markets:evidence from MENA countries
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作者 Walid Mensi Debasish Maitra +1 位作者 Refk Selmi Xuan Vinh Vo 《Financial Innovation》 2023年第1期1449-1475,共27页
This study addresses whether gold exhibits the function of a hedge or safe haven as often referred to in academia.It contributes to the existing literature by(i)revisiting this question for the principal stock markets... This study addresses whether gold exhibits the function of a hedge or safe haven as often referred to in academia.It contributes to the existing literature by(i)revisiting this question for the principal stock markets in the Middle East and North Africa(MENA)region and(ii)using the copula-quantile-on-quantile and conditional value at risk methods to detail the risks facing market participants provided with accurate information about various gold and stock market scenarios(i.e.,bear,normal,bull).The results provide strong evidence of quantile dependence between gold and stock returns.Positive correlations are found between MENA gold and stock markets when both are bullish.Conversely,when stock returns are bearish,gold markets show negative correlations with MENA stock markets.The risk spillover from gold to stock markets intensified during the global financial and European crises.Given the risk spillover between gold and stock markets,investors in MENA markets should be careful when considering gold as a safe haven because its effectiveness as a hedge is not the same in all MENA stock markets.Investors and portfolio managers should rebalance their portfolio compositions under various gold and stock market conditions.Overall,such precise insights about the heterogeneous linkages and spillovers between gold and MENA stock returns provide potential input for developing effective hedging strategies and optimal portfolio allocations. 展开更多
关键词 Copula CoVaR Extreme dependence GOLD mena markets Risk spillovers
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Mena表达及SNPs与胃癌遗传易感性及预后 被引量:3
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作者 王密 张声 +5 位作者 任彩虹 陈祥娜 王行富 黎三艳 陈余朋 曾赛凡 《中国肿瘤临床》 CAS CSCD 北大核心 2013年第16期965-970,共6页
目的:探讨Mena表达与胃癌侵袭转移的相关性及SNPs与胃癌遗传易感性的关系。方法:制作模拟胃癌侵袭转移过程的组织芯片,免疫组化染色检测Mena蛋白的表达。PCR—LDR技术检测Mena基因5个SNPs位点多态性并行测序验证。结果:胃腺癌中Men... 目的:探讨Mena表达与胃癌侵袭转移的相关性及SNPs与胃癌遗传易感性的关系。方法:制作模拟胃癌侵袭转移过程的组织芯片,免疫组化染色检测Mena蛋白的表达。PCR—LDR技术检测Mena基因5个SNPs位点多态性并行测序验证。结果:胃腺癌中Mena表达上调,肠型与混合型胃癌高于弥漫型,并与胃癌侵袭转移负相关,Mena高表达者预后好。Mena基因SNP位点rs3795443的188例对照组等位基因A、G的频率分别为91.0%和9.0%,胃癌病例组等位基因A、c的频率为82.4%和17.6%;AA、A/G和GG的基因型频率在对照组分别为81.9%、18.1%和0,而在病例组为68.35%、28.09%和3.56%,差异具有统计学意义(OR=2.1489,95%CI:1.4607~3.1613,P〈0.01)。5个SNPs位点的基因型和等位基因频率与胃癌术后生存时间均无明显相关性。结论:胃癌Mena表达升高,组织学类型的维持以及侵袭转移与其表达密切相关,高表达者预后好。MenaSNPrs3795443位点携带等位基因G的GG和A/G基因型个体的胃癌患病风险提高,提示检测该位点基因型有助于评估胃癌的遗传易感性。 展开更多
关键词 胃癌 mena表达 SNP 传易感性 预后
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大肠杆菌menA敲除对CoQ积累的影响研究
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作者 刘永青 任琳 张子锋 《生物技术通报》 CAS CSCD 北大核心 2015年第12期207-213,共7页
通过同源重组敲除大肠杆菌的men A基因,增加菌体Co Q合成量,用于构建Co Q高产菌株。以p KD4质粒为模板,PCR扩增kanr片段;在p KD46的辅助下,kanr片段转化大肠杆菌,利用抗生素筛选和PCR验证重组子;以紫外诱变菌为对照,发酵men A基因敲除菌... 通过同源重组敲除大肠杆菌的men A基因,增加菌体Co Q合成量,用于构建Co Q高产菌株。以p KD4质粒为模板,PCR扩增kanr片段;在p KD46的辅助下,kanr片段转化大肠杆菌,利用抗生素筛选和PCR验证重组子;以紫外诱变菌为对照,发酵men A基因敲除菌株,分析Co Q种类和产量变化。成功获得men A基因敲除菌株,Co Q种类不变,产量增加约为38%。首次敲除men A基因,改良株Co Q产量得到提高,达到预期目标。 展开更多
关键词 大肠杆菌 基因敲除 mena基因 COQ 同源重组
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Mena蛋白在大肠癌及癌旁组织、息肉、淋巴结等中的表达及意义
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作者 孙威 裴笑月 +2 位作者 马帅 贾翼 于剑 《中国医药指南》 2019年第3期135-136,共2页
目的观察Mena蛋白在大肠癌及癌旁组织、息肉(腺瘤)、正常淋巴结及转移淋巴结中的表达,探讨其临床意义。方法免疫组化染色检测大肠癌及癌旁组织、息肉(腺瘤)、正常淋巴结及转移淋巴结各20例中Mena蛋白的表达,观察Mena蛋白与分化程度、组... 目的观察Mena蛋白在大肠癌及癌旁组织、息肉(腺瘤)、正常淋巴结及转移淋巴结中的表达,探讨其临床意义。方法免疫组化染色检测大肠癌及癌旁组织、息肉(腺瘤)、正常淋巴结及转移淋巴结各20例中Mena蛋白的表达,观察Mena蛋白与分化程度、组织分型及肿瘤标志物的关系,并分析其临床意义。结果大肠癌组织中的Mena蛋白表达最高,其他依次为转移淋巴结、息肉(腺瘤)及癌旁组织,正常淋巴结中不表达;Mena蛋白表达与分化程度、组织分型及肿瘤标志物有关,与年龄、性别及部位无关。结论大肠癌中的Mena过表达,组织学类型及侵袭转移与其表达密切相关。 展开更多
关键词 mena表达 大肠癌 癌旁组织 息肉 淋巴结 意义
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TES通过Mena依赖的方式抑制胃癌生长和转移 被引量:3
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作者 王丹丹 陈义兵 +9 位作者 赵靖靖 张晓菲 朱广超 翁德胜 潘科 吕琳 潘求忠 江珊珊 王磊磊 夏建川 《癌症》 SCIE CAS CSCD 2020年第7期321-334,共14页
背景与目的在前期研究中,我们在原发性胃癌(gastric cancer,GC)中发现了一个候选抑癌基因——TES(testin LIM domain protein)。TES包括3个LIM结构域,后者是与细胞黏附和细胞骨架调节蛋白发生特异性相互作用的区域。Mena是一种已知的细... 背景与目的在前期研究中,我们在原发性胃癌(gastric cancer,GC)中发现了一个候选抑癌基因——TES(testin LIM domain protein)。TES包括3个LIM结构域,后者是与细胞黏附和细胞骨架调节蛋白发生特异性相互作用的区域。Mena是一种已知的细胞骨架调节因子,可通过与片状伪足(lamellipodin,Lpd)相互作用调节肌动蛋白丝的组装并调节细胞黏附与运动。因此,我们假设TES在胃癌中通过与Mena相互作用发挥抑癌因子功能。本研究旨在探索TES在胃癌中的肿瘤抑制功能。方法我们通过体外细胞增殖实验、克隆形成实验、细胞周期分析、转移实验和体内肿瘤形成与转移试验来研究TES在胃癌中的抑癌作用。采用免疫沉淀–质谱法研究TES和Mena的相互作用。我们采用免疫组化分析了172例胃癌样本中TES和Mena的表达情况,并研究了胃癌中TES和Mena的临床病理特征及预后意义。结果TES抑制胃癌细胞的增殖和克隆形成,导致细胞周期阻滞,并在裸鼠内抑制胃癌细胞的成瘤性。此外,TES还可以抑制胃癌细胞在体外的迁移和侵袭,同时抑制体内肿瘤转移。TES与Mena相互作用,抑制了Mena与Lpd之间的相互作用。Transewll实验表明,TES通过Mena依赖方式抑制胃癌细胞的迁移和侵袭。在Mena高表达的胃癌患者中,TES的表达与肿瘤浸润(P=0.005)、淋巴结转移(P=0.003)、TNM分期(P=0.003)及预后(P=0.010)相关。但在Mena低表达的胃癌患者中无以上相关性。结论我们认为TES通过Mena依赖的方式发挥抑癌作用。TES可作为一种有价值的胃癌预后标志物及潜在的治疗靶标。 展开更多
关键词 胃癌 TES 抑癌因子 mena 片状伪足
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The Middle East and North Africa (MENA) Oil Exporters After the Global Crisis
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作者 Naci Yllmaz Bora Selcuk 《Chinese Business Review》 2012年第7期627-638,共12页
Our research topic is about the economic situation in the Middle East and North Africa (MENA) oil exporters after the global crisis in 2008. The authors tried to find the answers to the questions such as "How bad o... Our research topic is about the economic situation in the Middle East and North Africa (MENA) oil exporters after the global crisis in 2008. The authors tried to find the answers to the questions such as "How bad or good is the situation there?" and "what is the expectation about the future of the region?" Like other countries around the world, the MENA oil exporters, comprised of Algeria, Bahrain, Iran, Iraq, Kuwait, Libya, Oman, Qatar, Saudi Arabia, Sudan, United Arab Emirates, Yemen were adversely affected by the global crisis. The MENA oil exporters are recently recovering from their recession in 2009 when the global crisis was felt intensively there. GDP in the region has grown by 3.5 percent in 2010 after a low growth of 0.7 percent in 2009. The average real GDP growth excluding Libya is projected to reach 4.9 percent in 2011. Two developments mark the outlook: the unrest in the region and the surge in global fuel and food prices. For most oil exporters, the expected increase in oil prices and production volumes will lead to higher growth in 2011 and stronger fiscal and external balances, notwithstanding recent increases in government spending. The oil exporters' combined external current account surplus is estimated to increase. The effects of the political events in Tunisia and Egypt have spread in varying degrees to the MENA oil exporters in the region, and many of these countries are responding with public spending and job-creation measures to alleviate social tensions. The social unrest also highlights the need to pursue fundamental economic reforms--social policy, fiscal management, governance, business environment, labor markets, and financial sector access to facilitate more inclusive economic growth. While the MENA countries are generally divided into two sub-regions such as "oil exporters" and "oil importers" in the economic literature, the authors will deal with only the MENA oil exporters in this work. The negative effects of the global crisis in these countries on growth, inflation, current account balance, budget balance, and finally financial sector will be reviewed in this work and the expectations and the developments which occurred after the crisis in these countries will be dealt with especially in the context of the IMF reports. 展开更多
关键词 global crisis the Middle East and North Africa mena mena oil exporters
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Mena、Her-2蛋白在乳腺癌组织中的表达和意义
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作者 张青山 徐正顺 郑海燕 《陕西医学杂志》 CAS 2011年第5期613-615,633,共4页
目的:探讨乳腺癌组织中Mena和人表皮生长因子受体2(Her-2)蛋白的表达和意义。方法:采用免疫组化方法检测60例乳腺癌组织和15例正常乳腺组织中Mena和Her-2蛋白的表达,同时分析其与临床病理资料的关系。结果:Mena蛋白在乳腺癌组织中的阳... 目的:探讨乳腺癌组织中Mena和人表皮生长因子受体2(Her-2)蛋白的表达和意义。方法:采用免疫组化方法检测60例乳腺癌组织和15例正常乳腺组织中Mena和Her-2蛋白的表达,同时分析其与临床病理资料的关系。结果:Mena蛋白在乳腺癌组织中的阳性表达率为73.3%(44/60),Her-2蛋白的阳性表达率为38.3%(23/60),均明显高于在正常乳腺组织中(0.0%)的表达(P<0.05),且均与临床分期及腋淋巴结转移密切相关(P<0.05),与患者年龄、肿瘤大小、雌激素受体(ER)、孕激素受体(PR)无关(P>0.05);Mena与Her-2蛋白的表达有相关性(r=0.398,P<0.01)。结论:Mena和Her-2可能在乳腺癌细胞的浸润和转移中起重要作用;检测Mena和Her-2蛋白的表达可能对判断乳腺癌的恶性程度、浸润转移潜能及预后判断有重要参考价值。 展开更多
关键词 乳腺肿瘤/病理学 蛋白质类/代谢 受体 表皮生长因子/代谢 免疫组织化学 @mena
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Comparison of WRF Model Physics Parameterizations over the MENA-CORDEX Domain 被引量:3
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作者 George Zittis Panos Hadjinicolaou Jos Lelieveld 《American Journal of Climate Change》 2014年第5期490-511,共22页
We investigated the performance of 12 different physics configurations of the climate version of the Weather, Research and Forecasting (WRF) Model over the Middle East and North Africa (MENA) domain. Possible combinat... We investigated the performance of 12 different physics configurations of the climate version of the Weather, Research and Forecasting (WRF) Model over the Middle East and North Africa (MENA) domain. Possible combinations among two Planetary Boundary Layer (PBL), three Cumulus (CUM) and two Microphysics (MIC) schemes were tested. The 2-year simulations (December 1988-November 1990) have been compared with gridded observational data and station measurements for several variables, including total precipitation and maximum and minimum 2-meter air temperature. An objective ranking method of the 12 different simulations and the selection procedure of the best performing configuration for the MENA domain are based on several statistical metrics and carried out for relevant sub-domains and individual stations. The setup for cloud microphysics is found to have the strongest impact on temperature biases while precipitation is most sensitive to the cumulus parameterization scheme and mainly in the tropics. 展开更多
关键词 WRF Physics PARAMETERIZATIONS Regional Climate Modeling Sensitivity mena CORDEX
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Linking Environmental Water Scarcity and Options for Adaptation in the MENA Region
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作者 Bachar Ibrahim Henry Mensah 《Journal of Water Resource and Protection》 2017年第4期378-392,共15页
Contamination of surface and underground water by sea level rise, surface runoff, and land use activities such as industrial and agricultural activities can lead to water scarcity. Water could be available and accessi... Contamination of surface and underground water by sea level rise, surface runoff, and land use activities such as industrial and agricultural activities can lead to water scarcity. Water could be available and accessible but not suitable for human use. In the Middle East and North Africa (MENA) region countries, the agriculture sector depends massively on water for farming activities, which consumes about 80% of the available water resources. In this context, the issue of environmental water scarcity is under highlighted, though the use of this term itself is still lacking in an international context (FAO). Meanwhile, the international goal of universal access to clean freshwater is included in global development targets (SDGs). This study provides some insights and deepens our understanding on environmental water scarcity, particularly MEN-A region countries. This review paper begins with an introduction to water scarcity and continues with a discussion of environmental issues associated with water scarcity in MENA region countries. Finally, we suggest some adaptive measures in two distinct areas such as agriculture sectors and policy makers and conclude that the lack of implementation of this particular term is hindering sustainable development in MENA region countries. 展开更多
关键词 ADAPTATION mena Climate Change WATER SCARCITY WATER Quality Environment
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Water Shortage in MENA Region: An Interdisciplinary Overview and a Suite of Practical Solutions
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作者 Anas Zyadin 《Journal of Water Resource and Protection》 2013年第4期49-58,共10页
The chronic water shortage in the Middle East North Africa (MENA) region is a perplexing issue, undoubtedly because various operational sectors, multi-institutions and stakeholders are inextricably interconnected. In ... The chronic water shortage in the Middle East North Africa (MENA) region is a perplexing issue, undoubtedly because various operational sectors, multi-institutions and stakeholders are inextricably interconnected. In the light of climate variability and the unprecedented population growth rate, the per capita water resources and biocapacity will continue plummeting, and the demand-gap will seriously expand. Existing water quantification agreements have been deemed inefficient to solve the problem of naturally diminishing water resources and thus require immediate re-assembling. Most scholarly endeavors, including key international organizations, NGOs, and “Think Tank” policy briefs have limitedly addressed water shortage in contexts of regional politics, mass media, and, importantly, from social psychology perspectives. Therefore, a thorough analysis and interdisciplinary approach is required to find a feasible and suitable framework of solutions and from a multi-perspective podium. A synthesis of cross-sectorial bottlenecks that are crucial to water management is presented, and a suite of practical recommendations are introduced to water authorities and governments. This study argues that in the shadow of the region’s political instability, the clash of ideologies and its repercussions, and issues of national security and sovereignty, regional cooperation on water issues remain prognostications. In this essence, governments of MENA countries are urged to develop measures to substantially increase the water supply through innovative approaches. Such measures include enhancing the capacities of water harvesting, maximizing the storage capacities of the built dams, and deploying groundwater recharge techniques. Furthermore, seawater and brackish water desalination through clean energy technologies is a contemporary solution with socio-economic and multiple benefits. Multi-billion water projects might not be suitable approach in the absence of external funding and the aforementioned hurdles. Further research is required to address the social economics, and environmental aspects of desalination and the socio-economic feasibility of privatizing drinking water utilities and price polarization. 展开更多
关键词 Water Conflicts RENEWABLE Energy mena REGION INTERDISCIPLINARY Approach
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Mena蛋白与TES蛋白在肿瘤侵袭转移中的作用研究进展
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作者 齐学袖 《药学研究》 CAS 2019年第6期351-354,共4页
Mena蛋白是Ena/VASP家族蛋白之一,在细胞伪足的形成和延伸、细胞的运动中起着重要的作用;TES是一种细胞黏附分子,在细胞外抑制肿瘤细胞的迁移和侵袭。近年来研究表明,肿瘤细胞伪足的形成和延伸以及细胞黏附是影响肿瘤细胞迁移和侵袭的... Mena蛋白是Ena/VASP家族蛋白之一,在细胞伪足的形成和延伸、细胞的运动中起着重要的作用;TES是一种细胞黏附分子,在细胞外抑制肿瘤细胞的迁移和侵袭。近年来研究表明,肿瘤细胞伪足的形成和延伸以及细胞黏附是影响肿瘤细胞迁移和侵袭的主要原因,本文通过探讨Mena蛋白与TES蛋白相互作用对肿瘤细胞伪足和细胞运动能力以及细胞黏附的影响,阐明Mena蛋白与TES蛋白在肿瘤细胞迁移和侵袭过程中的作用及分子机制,为肿瘤转移患者分子靶向治疗提供新的治疗靶点策略和思路。 展开更多
关键词 mena蛋白 TES蛋白 相互作用 肿瘤 侵袭 转移
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Water Demand Management Is a Must in MENA Countries…But Is It Enough?
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作者 Wael Mualla 《Journal of Geological Resource and Engineering》 2018年第2期59-64,共6页
The majority of Middle East and North Africa(MENA)countries suffer from chronic imbalance between available water supply and rising water demand.This imbalance is expected to worsen even further in the future as a res... The majority of Middle East and North Africa(MENA)countries suffer from chronic imbalance between available water supply and rising water demand.This imbalance is expected to worsen even further in the future as a result of sharp population growth,rapid economic development and climate change,unless major positive measures are implemented to augment water supply and manage water demand.The supply management approach,on its own,practiced by many countries in the region for so many years has so far demonstrated its inability to bridge the“water gap”between available water resources and rising water demand,as most traditional water resourced in almost all MENA countries have been exploited(or over exploited),and the cost of non-traditional water resources has become increasingly prohibitively high,apart from its environmental impact.Demand management is regarded by many water experts in the region as the answer or“panacea”for the water imbalance problem.But,is demand management approach alone able to solve the problem of water scarcity in the MENA region?In other words,if all demand management measures have been fully implemented,would there still be gaps between supply and demand that need to be filled with supply augmentation,andwill supply management options still need to be part of the solution?This paper tries to answer this question by reviewing several works in this domain,particularly,recent studies by the World Bank[9-11].It was concluded that,although water demand management measures should be given the first priority,especially,in the agricultural sector where it has the maximum impact,demand management on its own will not be able to bridge the“water gap”,and supply management options,such as sea water desalination and the re-use of treated wastewater,will be part of the solution. 展开更多
关键词 mena COUNTRIES demand management WATER SCARCITY WATER gap
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The Middle Eastern Eagle: How the MENA Region Can Rise
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作者 Anna Cornelia Beyer 《International Relations and Diplomacy》 2014年第2期144-149,共6页
The Middle Eastern and North African (MENA) region has the potential to rise if it puts the recent changes to good use. Political changes in some countries might have opened the way for liberalization, which in turn... The Middle Eastern and North African (MENA) region has the potential to rise if it puts the recent changes to good use. Political changes in some countries might have opened the way for liberalization, which in turn again might facilitate democratization. Both will benefit the economic development of the region. So far, economic growth is hindered by excessive and ineffective public sectors, which do not provide sufficient opportunities for the growing young population. Privatization of some services and a general liberalization of the markets might help bring the young workforce into employment and in the long run will fuel an economic upswing. 展开更多
关键词 Middle Eastern and North African mena economic DEMOCRACY
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Renewable Energy Technologies Penetration in MENA Region (2010-2030)
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作者 Marc Anthony Mannah Loren Makki +2 位作者 Ahmad Haddad Hassan Bazzi Ali Hammoud 《Journal of Electrical Engineering》 2016年第2期100-107,共8页
This paper focuses on quantifying the renewable energy mix in the MENA (Middle East and North Africa) region and underlines its impact in terms of economic aspects. The installed and targeted capacities of solar, wi... This paper focuses on quantifying the renewable energy mix in the MENA (Middle East and North Africa) region and underlines its impact in terms of economic aspects. The installed and targeted capacities of solar, wind and other technologies will be revealed. In addition, distinguished present and on-going projects as well as prominent funding organizations are discussed. The overall strategy as well as the evolution of the renewable energy plan is resumed. 展开更多
关键词 mena renewable energy solar energy wind energy funding organizations.
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Youth voting and institutional change in the post-Arab Spring MENA region
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作者 Ali Fakih Mohammad Hammoud Nagham Sayour 《Economic and Political Studies》 2024年第3期318-344,共27页
Using a unique and novel dataset on the youth,the SAHWA Youth Survey(2016),we apply probit and ordered probit models to study the determinants of voting behaviour change among the youth in the Middle East and North Af... Using a unique and novel dataset on the youth,the SAHWA Youth Survey(2016),we apply probit and ordered probit models to study the determinants of voting behaviour change among the youth in the Middle East and North Africa(MENA)region in the post-Arab Spring era.We find that drivers of voting vary depending on whether we consider the voting behaviour in the last elections or the likelihood of voting in the next elections.Specifically,socioeconomic variables and some Arab Spring factors are significant for both types of elections.However,institutional variables and personal beliefs only affect the likelihood of voting in the next elections.We also document heterogeneous effects for the last and next votes by gender. 展开更多
关键词 Voting behaviour YOUTH Arab Spring institutional change the mena region
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纳豆芽孢杆菌MenA的生物信息学分析及亚细胞定位 被引量:4
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作者 丁秀敏 薛正莲 +6 位作者 王洲 杨自名 冯静静 吴佳雯 胡润 章宁娟 刘艳 《基因组学与应用生物学》 CAS CSCD 北大核心 2020年第10期4604-4613,共10页
为了深入研究纳豆芽孢杆菌中1,4-二羟基-2-萘甲酸聚异戊二烯转移酶(BsnMenA)的结构和功能、催化作用机制以及亚细胞定位,本研究首先利用生物信息学软件对BsnMenA的系统进化关系、基本性质、亚细胞定位、结构域和motifs以及空间结构等进... 为了深入研究纳豆芽孢杆菌中1,4-二羟基-2-萘甲酸聚异戊二烯转移酶(BsnMenA)的结构和功能、催化作用机制以及亚细胞定位,本研究首先利用生物信息学软件对BsnMenA的系统进化关系、基本性质、亚细胞定位、结构域和motifs以及空间结构等进行预测。另外还构建了纳豆芽孢杆菌menA和egfp荧光标签的重组菌MEP28和EMP28,通过IPTG诱导表达后,利用激光共聚焦显微镜观察重组菌中荧光所在的位置。系统进化树显示,芽孢杆菌属中的MenA高度相似,亲缘关系较近,但与大肠杆菌等其它科中的MenA亲缘关系较远。从理化性质及亚细胞定位预测,初步确定BsnMenA属于膜结合型蛋白质。结构域和motifs预测显示,BsnMenA的34-278aa组成典型的UbiA结构功能域,其中74-84aa和204-212aa分别存在NxxxDxxxxxD和NNxxDxxxD的Asp-rich motifs结构。通过折叠识别建模法,模拟出BsnMenA的三维空间结构,是由9个跨膜螺旋组成的一个活性中心腔,其中Asp-rich motifs位于胞质区loop的H23和H67中,这与匹配度最高的嗜热系古生细菌中的4-羟苯甲酸-聚异戊二烯转移酶(ApUbiA)极其相似,由于BsnMenA与ApUbiA空间结构高度相似,因此推导出BsnMenA的催化功能有关的保守结构域位点及其催化机制。对重组菌诱导表达20 min后取样,通过激光共聚焦显微镜检测发现,在细菌的细胞膜上有明显可见荧光。通过生物信息学预测以及亚细胞定位实验不仅验证BsnMenA是膜蛋白酶,而且说明BsnMenA能在大肠杆菌中异源表达,这些理论为BsnMenA的深入研究提供理论基础,也为其它未知晶体结构酶的研究提供了有效的研究思路和方法。 展开更多
关键词 纳豆芽孢杆菌(Bacillus SUBTILIS natto) 异戊烯转移酶mena 生物信息学分析 亚细胞定位
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TES functions as a Mena-dependent tumor suppressor in gastric cancer carcinogenesis and metastasis 被引量:2
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作者 Dan-dan Wang Yi-bing Chen +9 位作者 Jing-jing Zhao Xiao-fei Zhang Guang-chao Zhu De-sheng Weng Ke Pan Lin Lv Qiu-zhong Pan Shan-shan Jiang Lei-lei Wang Jian-chuan Xia 《Cancer Communications》 SCIE 2019年第1期14-27,共14页
Background: In our previous study, we identified a candidate tumor suppressor gene, testin LIM domain protein (TES), in primary gastric cancer (GC). TES contains three LIM domains, which are specific interacting regio... Background: In our previous study, we identified a candidate tumor suppressor gene, testin LIM domain protein (TES), in primary gastric cancer (GC). TES contains three LIM domains, which are specific interacting regions for the cell adhesion and cytoskeleton regulatory proteins. Mena is a known cytoskeleton regulator that regulates the assembly of actin filaments and modulates cell adhesion and motility by interacting with Lamellipodin (Lpd). Therefore, we hypothesized that TES plays a role as tumor suppressor in GC through interacting with Mena. This study aimed to investigate the tumor suppressive functions of TES in GC. Methods: We explored the tumor suppressive effect of TES in GC by in vitro cell proliferation assay, colony formation assay, cell cycle analysis, Transwell assays, and in vivo tumorigenicity and metastasis assays. The interaction of TES and Mena was investigated through immunoprecipitation-based mass spectrometry. We also analyzed the expression of TES and Mena in 172 GC specimens using immunohistochemistry and investigated the clinicopathological and prog-nostic significance of TES and Mena in GC. Results: TES suppressed GC cell proliferation and colony formation, induced cell cycle arrest, and inhibited tumorigenicity in vitro. Additionally, it inhibited GC cell migration and invasion in vitro and suppressed metastasis in vivo. TES interacted with Mena, and inhibited the interaction of Mena with Lpd. Transwell assays suggested that TES suppressed migration and invasion of GC cells in a Mena-dependent fashion. In GC patients with high Mena expression, the expression of TES was associated with tumor infiltration (P = 0.005), lymph node metastasis (P = 0.003), TNM stage (P = 0.003), and prognosis (P = 0.010). However, no significant association was observed in GC patients with low Mena expression. Conclusions: We believe that TES functions as a Mena-dependent tumor suppressor. TES represents a valuable prog-nostic marker and potential target for GC treatment. 展开更多
关键词 Gastric cancer TES Tumor suppressor mena Lamellipodin
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七烯甲萘醌合成过程中关键蛋白的定位分析
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作者 陈奇 张智航 +5 位作者 夏洪志 孙怡 金柯 吕雪芹 崔世修 刘龙 《食品与发酵工业》 CAS CSCD 北大核心 2023年第2期8-12,共5页
七烯甲萘醌作为维生素K 2的重要亚型,在细胞中主要存在于细胞膜上,在氧化磷酸化的过程中起到电子载体的作用。作为七烯甲萘醌合成过程中的关键酶,1,4-二羟基-2-萘甲酸异戊二烯基转移酶(1,4-dihydroxy-2-naphthoic acid prenyltransferas... 七烯甲萘醌作为维生素K 2的重要亚型,在细胞中主要存在于细胞膜上,在氧化磷酸化的过程中起到电子载体的作用。作为七烯甲萘醌合成过程中的关键酶,1,4-二羟基-2-萘甲酸异戊二烯基转移酶(1,4-dihydroxy-2-naphthoic acid prenyltransferase,MenA)和甲基转移酶(methyltransferase,MenH)目前研究较少,其在细胞中的分布还未被研究,这也限制了七烯甲萘醌产量的进一步提高。该研究重点关注MenA和MenH在细胞内的分布问题,首先构建MenA、MenH和eGFP的融合蛋白,利用荧光定位的方法确定MenA和MenH在细胞中的定位。然后,使用表面活性剂Triton-100破坏细胞膜的结构,发现随着表面活性剂浓度的增加,七烯甲萘醌的合成受到明显抑制。该研究表明细胞膜结构的完整性是七烯甲萘醌合成的前提条件,并且揭示了七烯甲萘醌是在细胞膜上直接被合成,不是被转运到细胞膜中。研究结果为深入探索七烯甲萘醌合成机制提供了方向。 展开更多
关键词 七烯甲萘醌 枯草芽孢杆菌 mena MenH 细胞膜 定位
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哺乳动物肌动蛋白调节蛋白在肿瘤进展和转移中的作用
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作者 王密 《中国现代医生》 2011年第23期32-33,共2页
哺乳动物肌动蛋白调节蛋白enabled(Mena)是近年来发现的新基因,在多种肿瘤中过表达。Mena与细胞运动性和黏附性的调节相关。Mena的过表达诱导细胞膜突起和增加肿瘤细胞对基质的降解活性,增加体内癌细胞的细胞能动性,促进癌细胞的侵袭和... 哺乳动物肌动蛋白调节蛋白enabled(Mena)是近年来发现的新基因,在多种肿瘤中过表达。Mena与细胞运动性和黏附性的调节相关。Mena的过表达诱导细胞膜突起和增加肿瘤细胞对基质的降解活性,增加体内癌细胞的细胞能动性,促进癌细胞的侵袭和转移。本文对其生化结构、在正常和肿瘤组织中的表达定位以其在肿瘤发生发展中的作用研进行综述。 展开更多
关键词 mena 肿瘤 侵袭 转移
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2004年12月26日印尼8.7级强震后江苏地区同震效应分析 被引量:19
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作者 郑江蓉 徐桂明 +1 位作者 李鸿宇 唐振芳 《防灾减灾工程学报》 CSCD 2005年第1期105-113,共9页
在2004年12月26日印尼苏门答腊北部发生8.7级强烈地震后,对江苏省地震前兆观测资料进行了及时的清理和分析,发现凡是能监测地下应力活动的前兆观测手段,如应变、地倾斜、水温和水位,在这次特大地震的震后都记录到了显著的异常,且异常的... 在2004年12月26日印尼苏门答腊北部发生8.7级强烈地震后,对江苏省地震前兆观测资料进行了及时的清理和分析,发现凡是能监测地下应力活动的前兆观测手段,如应变、地倾斜、水温和水位,在这次特大地震的震后都记录到了显著的异常,且异常的表现形式各不相同,将这些异常列出并浅析之,以期对进一步深入研究这次地震对我国未来地震趋势的影响有所帮助。 展开更多
关键词 印尼8.7级强烈地震 前兆观测 同震异常
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