磷酸氢钙反应是制备磷酸盐产品的重要工艺步骤之一。MER值(Magnesium to Extractable Phosphorus Ratio)作为一个衡量磷酸氢钙反应过程中杂质含量的指标,对反应过程的影响引起了广泛的关注。通过研究液相中的反应物扩散过程、杂质产物...磷酸氢钙反应是制备磷酸盐产品的重要工艺步骤之一。MER值(Magnesium to Extractable Phosphorus Ratio)作为一个衡量磷酸氢钙反应过程中杂质含量的指标,对反应过程的影响引起了广泛的关注。通过研究液相中的反应物扩散过程、杂质产物对固体膜和液体膜的影响、磷酸电离与氢离子活度变化的关系以及镁离子对反应速率和能耗的影响,探讨了MER值对磷酸氢钙反应过程的影响机制。实验结果表明,较高的MER值会导致反应物扩散受阻、杂质产物影响反应进行、磷酸电离减弱以及反应速率降低。因此,在工业生产中,需要控制和优化MER值,以提高反应过程的效果和产物的纯度。这些研究结果对于磷酸氢钙反应的工艺改进和优化具有重要的指导意义。展开更多
A research team jointly led by Dr.SHI Zhengli and Dr.ZHOU Peng from the CAS Wuhan Institute of Virology and their collaborators have discovered a novel MERS-like coronavirus in Malayan pangolins,the world’s most traf...A research team jointly led by Dr.SHI Zhengli and Dr.ZHOU Peng from the CAS Wuhan Institute of Virology and their collaborators have discovered a novel MERS-like coronavirus in Malayan pangolins,the world’s most trafficked mammals(doi:10.1016/j.cell.2023.01.019).展开更多
磁能恢复开关MERS(magnetic energy recovery switch)是一种新型的无功补偿装置,它相较于其他无功补偿装置,具有结构简单、易于控制和损耗低等诸多优点,但在进行无功功率补偿的过程中会产生谐波电流。首先,在对MERS的结构和控制方法阐...磁能恢复开关MERS(magnetic energy recovery switch)是一种新型的无功补偿装置,它相较于其他无功补偿装置,具有结构简单、易于控制和损耗低等诸多优点,但在进行无功功率补偿的过程中会产生谐波电流。首先,在对MERS的结构和控制方法阐述的基础上建立了三相对称系统中SVC-MERS的数学模型,讨论分析了控制参数对三相对称系统中线电流谐波特性的影响;进而提出了控制参数的优化设计方法。仿真和实验结果表明,通过控制参数的优化设计可以有效地减小三相对称系统中线电流的谐波含量,所提控制参数的优化设计方法是可行的。展开更多
Background: Middle East respiratory syndrome coronavirus(MERS-Co V), which is not fully understood in regard to certain transmission routes and pathogenesis and lacks specific therapeutics and vaccines, poses a global...Background: Middle East respiratory syndrome coronavirus(MERS-Co V), which is not fully understood in regard to certain transmission routes and pathogenesis and lacks specific therapeutics and vaccines, poses a global threat to public health.Methods: To simulate the clinical aerosol transmission route, h DPP4 transgenic mice were infected with MERS-Co V by an animal nose-only exposure device and compared with instillation-inoculated mice. The challenged mice were observed for 14 consecutive days and necropsied on days 3, 5, 7, and 9 to analyze viral load, histopathology, viral antigen distribution, and cytokines in tissues.Results: MERS-Co V aerosol-infected mice with an incubation period of 5-7 days showed weight loss on days 7-11, obvious lung lesions on day 7, high viral loads in the lungs on days 3-9 and in the brain on days 7-9, and 60% survival. MERS-Co V instillation-inoculated mice exhibited clinical signs on day 1, obvious lung lesions on days 3-5, continuous weight loss, 0% survival by day 5, and high viral loads in the lungs and brain on days 3-5. Viral antigen and high levels of proinflammatory cytokines and chemokines were detected in the aerosol and instillation groups. Disease, lung lesion, and viral replication progressions were slower in the MERS-Co V aerosol-infected mice than in the MERS-Co V instillation-inoculated mice.Conclusion: h DPP4 transgenic mice were successfully infected with MERS-Co V aerosols via an animal nose-only exposure device, and aerosol-and instillation-infected mice simulated the clinical symptoms of moderate diffuse interstitial pneumonia. However, the transgenic mice exposed to aerosol MERS-Co V developed disease and lung pathology progressions that more closely resembled those observed in humans.展开更多
目的探讨关于中东呼吸综合征-冠状病毒(middle east respiratory syndrome-coronavirus,MERS-CoV)调控非小细胞肺癌细胞A549补体系统抑制因子补体成分4结合蛋白Alpha(complement component 4 binding protein alpha,C4BPA)的机制。方法...目的探讨关于中东呼吸综合征-冠状病毒(middle east respiratory syndrome-coronavirus,MERS-CoV)调控非小细胞肺癌细胞A549补体系统抑制因子补体成分4结合蛋白Alpha(complement component 4 binding protein alpha,C4BPA)的机制。方法以是否MERS-CoV感染非小细胞肺癌细胞A549分为感染组和对照组,通过酶联免疫吸附试验(ELISA)、免疫印迹和实时荧光定量PCR(qPCR)分别检测2组细胞C4BPA的蛋白和mRNA表达情况。TargetScanHuman预测与C4BPA结合的miRNA,实时荧光定量PCR和荧光素酶报告试验确定靶向C4BPA的miRNA。在MERS-CoV感染的情况下,过表达或敲低靶向C4BPA的miRNA,ELISA或qPCR检测C4BPA的表达和分泌情况。同时分析人肺成纤维细胞和小鼠感染MERS-CoV后,C4BPA和miR-451b的表达情况。结果非小细胞肺癌细胞A549感染MERS-CoV后,其分泌的C4BPA增加(P<0.05)。通过TargetScanHuman在线分析和qPCR发现MERS-CoV抑制非小细胞肺癌细胞A549 miR-451b的表达(P<0.05),并且miR-451b靶向C4BPA的3‘UTR(P<0.05)。过表达miR-451b后,C4BPA的表达和分泌受到抑制(P<0.05)。敲低miR-451b后,C4BPA的表达和分泌受到促进(P<0.05)。同时,MERS-CoV感染人肺成纤维细胞和小鼠后,miR-451b的表达受到抑制(P<0.05),C4BPA的表达增强。并且,MERSCoV感染引起的C4BPA增多能够抑制补体经典系统的溶血活性。结论MERS-CoV通过下调miR-451b促进C4BPA的分泌。展开更多
文摘磷酸氢钙反应是制备磷酸盐产品的重要工艺步骤之一。MER值(Magnesium to Extractable Phosphorus Ratio)作为一个衡量磷酸氢钙反应过程中杂质含量的指标,对反应过程的影响引起了广泛的关注。通过研究液相中的反应物扩散过程、杂质产物对固体膜和液体膜的影响、磷酸电离与氢离子活度变化的关系以及镁离子对反应速率和能耗的影响,探讨了MER值对磷酸氢钙反应过程的影响机制。实验结果表明,较高的MER值会导致反应物扩散受阻、杂质产物影响反应进行、磷酸电离减弱以及反应速率降低。因此,在工业生产中,需要控制和优化MER值,以提高反应过程的效果和产物的纯度。这些研究结果对于磷酸氢钙反应的工艺改进和优化具有重要的指导意义。
文摘A research team jointly led by Dr.SHI Zhengli and Dr.ZHOU Peng from the CAS Wuhan Institute of Virology and their collaborators have discovered a novel MERS-like coronavirus in Malayan pangolins,the world’s most trafficked mammals(doi:10.1016/j.cell.2023.01.019).
文摘磁能恢复开关MERS(magnetic energy recovery switch)是一种新型的无功补偿装置,它相较于其他无功补偿装置,具有结构简单、易于控制和损耗低等诸多优点,但在进行无功功率补偿的过程中会产生谐波电流。首先,在对MERS的结构和控制方法阐述的基础上建立了三相对称系统中SVC-MERS的数学模型,讨论分析了控制参数对三相对称系统中线电流谐波特性的影响;进而提出了控制参数的优化设计方法。仿真和实验结果表明,通过控制参数的优化设计可以有效地减小三相对称系统中线电流的谐波含量,所提控制参数的优化设计方法是可行的。
基金National Science and Technology Major Projects of Infectious Disease, Grant/Award Number: 2018ZX10734401-011
文摘Background: Middle East respiratory syndrome coronavirus(MERS-Co V), which is not fully understood in regard to certain transmission routes and pathogenesis and lacks specific therapeutics and vaccines, poses a global threat to public health.Methods: To simulate the clinical aerosol transmission route, h DPP4 transgenic mice were infected with MERS-Co V by an animal nose-only exposure device and compared with instillation-inoculated mice. The challenged mice were observed for 14 consecutive days and necropsied on days 3, 5, 7, and 9 to analyze viral load, histopathology, viral antigen distribution, and cytokines in tissues.Results: MERS-Co V aerosol-infected mice with an incubation period of 5-7 days showed weight loss on days 7-11, obvious lung lesions on day 7, high viral loads in the lungs on days 3-9 and in the brain on days 7-9, and 60% survival. MERS-Co V instillation-inoculated mice exhibited clinical signs on day 1, obvious lung lesions on days 3-5, continuous weight loss, 0% survival by day 5, and high viral loads in the lungs and brain on days 3-5. Viral antigen and high levels of proinflammatory cytokines and chemokines were detected in the aerosol and instillation groups. Disease, lung lesion, and viral replication progressions were slower in the MERS-Co V aerosol-infected mice than in the MERS-Co V instillation-inoculated mice.Conclusion: h DPP4 transgenic mice were successfully infected with MERS-Co V aerosols via an animal nose-only exposure device, and aerosol-and instillation-infected mice simulated the clinical symptoms of moderate diffuse interstitial pneumonia. However, the transgenic mice exposed to aerosol MERS-Co V developed disease and lung pathology progressions that more closely resembled those observed in humans.
文摘目的探讨关于中东呼吸综合征-冠状病毒(middle east respiratory syndrome-coronavirus,MERS-CoV)调控非小细胞肺癌细胞A549补体系统抑制因子补体成分4结合蛋白Alpha(complement component 4 binding protein alpha,C4BPA)的机制。方法以是否MERS-CoV感染非小细胞肺癌细胞A549分为感染组和对照组,通过酶联免疫吸附试验(ELISA)、免疫印迹和实时荧光定量PCR(qPCR)分别检测2组细胞C4BPA的蛋白和mRNA表达情况。TargetScanHuman预测与C4BPA结合的miRNA,实时荧光定量PCR和荧光素酶报告试验确定靶向C4BPA的miRNA。在MERS-CoV感染的情况下,过表达或敲低靶向C4BPA的miRNA,ELISA或qPCR检测C4BPA的表达和分泌情况。同时分析人肺成纤维细胞和小鼠感染MERS-CoV后,C4BPA和miR-451b的表达情况。结果非小细胞肺癌细胞A549感染MERS-CoV后,其分泌的C4BPA增加(P<0.05)。通过TargetScanHuman在线分析和qPCR发现MERS-CoV抑制非小细胞肺癌细胞A549 miR-451b的表达(P<0.05),并且miR-451b靶向C4BPA的3‘UTR(P<0.05)。过表达miR-451b后,C4BPA的表达和分泌受到抑制(P<0.05)。敲低miR-451b后,C4BPA的表达和分泌受到促进(P<0.05)。同时,MERS-CoV感染人肺成纤维细胞和小鼠后,miR-451b的表达受到抑制(P<0.05),C4BPA的表达增强。并且,MERSCoV感染引起的C4BPA增多能够抑制补体经典系统的溶血活性。结论MERS-CoV通过下调miR-451b促进C4BPA的分泌。
