跨国公司与当地政府关系(Multinational Corporation-Host Government Relations,简称MGR)已成为跨国公司进行海外投资决策时所考虑的一项战略问题。地方政府在开展对外招商,尤其是在当前全球金融危机的严峻形势下,加大对跨国公司的招...跨国公司与当地政府关系(Multinational Corporation-Host Government Relations,简称MGR)已成为跨国公司进行海外投资决策时所考虑的一项战略问题。地方政府在开展对外招商,尤其是在当前全球金融危机的严峻形势下,加大对跨国公司的招商工作力度时应充分考虑到MGR的重要性,通过跨国公司从事MGR的部门(通常是政府事务部或对外联络部)与这些公司建立良好的沟通渠道,争取对跨国公司招商工作的"事半功倍"效应。同时,地方政府应加强政府主动的公关活动,借助国际知名公关公司的专业包装和策划,提高当地在国际上的良好公关形象。展开更多
2′-O-methylation(Nm)is one of the most abundant RNA epigenetic modifications and plays a vital role in the post-transcriptional regulation of gene expression.Current Nm mapping approaches are normally limited to high...2′-O-methylation(Nm)is one of the most abundant RNA epigenetic modifications and plays a vital role in the post-transcriptional regulation of gene expression.Current Nm mapping approaches are normally limited to highly abundant RNAs and have significant technical hurdles in m RNAs or relatively rare non-coding RNAs(nc RNAs).Here,we developed a new method for enriching Nm sites by using RNA exoribonuclease and periodate oxidation reactivity to eliminate 2′-hydroxylated(2′-OH)nucleosides,coupled with sequencing(Nm-REP-seq).We revealed several novel classes of Nm-containing nc RNAs as well as m RNAs in humans,mice,and drosophila.We found that some novel Nm sites are present at fixed positions in different t RNAs and are potential substrates of fibrillarin(FBL)methyltransferase mediated by sno RNAs.Importantly,we discovered,for the first time,that Nm located at the 3′-end of various types of nc RNAs and fragments derived from them.Our approach precisely redefines the genome-wide distribution of Nm and provides new technologies for functional studies of Nm-mediated gene regulation.展开更多
Osteosarcoma(OS)patients have a poor prognosis due to its high degree of heterogeneity and high rate of metastasis.Magnetic hyperthermia therapy(MHT)combined with immunotherapy is an effective strategy to treat solid ...Osteosarcoma(OS)patients have a poor prognosis due to its high degree of heterogeneity and high rate of metastasis.Magnetic hyperthermia therapy(MHT)combined with immunotherapy is an effective strategy to treat solid and metastatic tumors.Here,we combined biodegradable magnesium(Mg)macroscale rods,which acted as an eddy thermo-magnetic agent under a low external alternating magnetic field,and immunotherapy to achieve a radical cure for OS.The eddy thermal effect(ETE)of the Mg rods(MgR)showed outstanding cytotoxic effects and enhanced the maturation of dendritic cells(DCs),and the mild MHT induced the immunogenic cell death(ICD)in the OS cells.Combined with immune checkpoint blockade(ICB)therapy,we obtained an excellent curative effect against OS,and a further evaluation demonstrated that the local MHT induced by the MgR increased T cells infiltration and the polarization of M1 macrophages.Interestingly,the biodegradable MgR also promoted bone osteogenesis.Our work highlighted the uneven ETE mediated by the biodegradable MgR induced a comprehensive immunologic activation in the OS tumor microenvironment(TME),which would inspire the application of MHT for the effective treatment of OS.展开更多
文摘跨国公司与当地政府关系(Multinational Corporation-Host Government Relations,简称MGR)已成为跨国公司进行海外投资决策时所考虑的一项战略问题。地方政府在开展对外招商,尤其是在当前全球金融危机的严峻形势下,加大对跨国公司的招商工作力度时应充分考虑到MGR的重要性,通过跨国公司从事MGR的部门(通常是政府事务部或对外联络部)与这些公司建立良好的沟通渠道,争取对跨国公司招商工作的"事半功倍"效应。同时,地方政府应加强政府主动的公关活动,借助国际知名公关公司的专业包装和策划,提高当地在国际上的良好公关形象。
基金supported by the National Key R&D Program of China(2019YFA0802202)the National Natural Science Foundation of China(91940304,31971228,31900903,31970604,32100467,32225011)the Youth Science and Technology Innovation Talent of Guangdong Te Zhi Plan(2019TQ05Y181)。
文摘2′-O-methylation(Nm)is one of the most abundant RNA epigenetic modifications and plays a vital role in the post-transcriptional regulation of gene expression.Current Nm mapping approaches are normally limited to highly abundant RNAs and have significant technical hurdles in m RNAs or relatively rare non-coding RNAs(nc RNAs).Here,we developed a new method for enriching Nm sites by using RNA exoribonuclease and periodate oxidation reactivity to eliminate 2′-hydroxylated(2′-OH)nucleosides,coupled with sequencing(Nm-REP-seq).We revealed several novel classes of Nm-containing nc RNAs as well as m RNAs in humans,mice,and drosophila.We found that some novel Nm sites are present at fixed positions in different t RNAs and are potential substrates of fibrillarin(FBL)methyltransferase mediated by sno RNAs.Importantly,we discovered,for the first time,that Nm located at the 3′-end of various types of nc RNAs and fragments derived from them.Our approach precisely redefines the genome-wide distribution of Nm and provides new technologies for functional studies of Nm-mediated gene regulation.
基金This article was partially supported by the National Natural Science Foundation of China(U20A20254,52072253,82172506,82030068)Collaborative Innovation Center of Suzhou Nano Science and Technology,the 111 Project,Joint International Research Laboratory of Carbon-Based Functional Materials and Devices,a Jiangsu Social Development Project(BE2019658)+1 种基金a Jiangsu Natural Science Fund for Distinguished Young Scholars(BK20211544)Suzhou Key Laboratory of Nanotechnology and Biomedicine.L.Cheng was supported by the Tang Scholarship of Soochow University.
文摘Osteosarcoma(OS)patients have a poor prognosis due to its high degree of heterogeneity and high rate of metastasis.Magnetic hyperthermia therapy(MHT)combined with immunotherapy is an effective strategy to treat solid and metastatic tumors.Here,we combined biodegradable magnesium(Mg)macroscale rods,which acted as an eddy thermo-magnetic agent under a low external alternating magnetic field,and immunotherapy to achieve a radical cure for OS.The eddy thermal effect(ETE)of the Mg rods(MgR)showed outstanding cytotoxic effects and enhanced the maturation of dendritic cells(DCs),and the mild MHT induced the immunogenic cell death(ICD)in the OS cells.Combined with immune checkpoint blockade(ICB)therapy,we obtained an excellent curative effect against OS,and a further evaluation demonstrated that the local MHT induced by the MgR increased T cells infiltration and the polarization of M1 macrophages.Interestingly,the biodegradable MgR also promoted bone osteogenesis.Our work highlighted the uneven ETE mediated by the biodegradable MgR induced a comprehensive immunologic activation in the OS tumor microenvironment(TME),which would inspire the application of MHT for the effective treatment of OS.
