[Objective] To investigate the genetic diversity, molecular phylogeny and origination in Wanbei cattle. [Methedl Blood samples were collected from 50 Wanbei cattle and 14 local cattle breeds. According to the bovine M...[Objective] To investigate the genetic diversity, molecular phylogeny and origination in Wanbei cattle. [Methedl Blood samples were collected from 50 Wanbei cattle and 14 local cattle breeds. According to the bovine MHC gene sequence, one pair of primers was designed and used for PCR amplification. The sequences of amplified MHC genes were aligned to detect MHC haplotypes and nucleotide polyrnorphisms. A phy- Iogenetic tree based on the MHC gene was also constructed. [Result] A total of 24 polymorphic loci covering 7.86% of the entire length of the se- quences and seven MHC haplotypes were detected. Among these polymorphic loci, there were 18 transitions, 1 tranversions and 5 transition / tran- version. The difference in nucleotide sequence was 0.26% -4.53% between the haplotypes. The nucleotide diversity （π value） and haplotype di- versity （H） were 0.012 - 0.019 and 0.602 - 0.617, respectively. [ Conclusion ] Wanbei cattle do not have rich MHC genetic diversity. They have closer aenetic relationship with Luxi cattle, and both are within the same sister group.展开更多
supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China （CARS-41）;the National High Technology Research and Development Program of China （2011AA100301）
In order to investigate the association of genotypes of HLA-DRB1 and HLA-DQB1 alleles with the genetic susceptibility of chronic urticaria (CU), genotypes of HLA-DRB1 and HLA-DQB1 genes were detected by polymerase cha...In order to investigate the association of genotypes of HLA-DRB1 and HLA-DQB1 alleles with the genetic susceptibility of chronic urticaria (CU), genotypes of HLA-DRB1 and HLA-DQB1 genes were detected by polymerase chain reactions with sequence-specific primers (PCR-SSP) in 42 patients with CU (19 men and 23 women, mean age 30.67±12.45 y old as well as 193 racially matched healthy persons in ethnic Han from Hubei provinece. Gene frequencies of HLA-DRB1*12, *0901 (RR=3.11, χ2=7.579, P=0.006; RR=2.47, χ2=5.684, P=0.017) were significantly increased in CU patients as compared with that in healthy people. Gene frequencies of HLA-DQB1*05 (RR=0.26, χ2=6.683, P=0.01) were significantly decreased in CU patients. It was suggested that CU was found strongly associated with HLA-DRB1*12, *0901 and HLA-DQB1*05, the former might be the genetic markers for susceptibility to CU, but the latter might play a resistive role.展开更多
基金supported by the Suzhou Key Technologies R&D Program ( 2009021)
文摘[Objective] To investigate the genetic diversity, molecular phylogeny and origination in Wanbei cattle. [Methedl Blood samples were collected from 50 Wanbei cattle and 14 local cattle breeds. According to the bovine MHC gene sequence, one pair of primers was designed and used for PCR amplification. The sequences of amplified MHC genes were aligned to detect MHC haplotypes and nucleotide polyrnorphisms. A phy- Iogenetic tree based on the MHC gene was also constructed. [Result] A total of 24 polymorphic loci covering 7.86% of the entire length of the se- quences and seven MHC haplotypes were detected. Among these polymorphic loci, there were 18 transitions, 1 tranversions and 5 transition / tran- version. The difference in nucleotide sequence was 0.26% -4.53% between the haplotypes. The nucleotide diversity （π value） and haplotype di- versity （H） were 0.012 - 0.019 and 0.602 - 0.617, respectively. [ Conclusion ] Wanbei cattle do not have rich MHC genetic diversity. They have closer aenetic relationship with Luxi cattle, and both are within the same sister group.
基金supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China (CARS-41)the National High Technology Research and Development Program of China (2011AA100301)
文摘supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China （CARS-41）;the National High Technology Research and Development Program of China （2011AA100301）
文摘In order to investigate the association of genotypes of HLA-DRB1 and HLA-DQB1 alleles with the genetic susceptibility of chronic urticaria (CU), genotypes of HLA-DRB1 and HLA-DQB1 genes were detected by polymerase chain reactions with sequence-specific primers (PCR-SSP) in 42 patients with CU (19 men and 23 women, mean age 30.67±12.45 y old as well as 193 racially matched healthy persons in ethnic Han from Hubei provinece. Gene frequencies of HLA-DRB1*12, *0901 (RR=3.11, χ2=7.579, P=0.006; RR=2.47, χ2=5.684, P=0.017) were significantly increased in CU patients as compared with that in healthy people. Gene frequencies of HLA-DQB1*05 (RR=0.26, χ2=6.683, P=0.01) were significantly decreased in CU patients. It was suggested that CU was found strongly associated with HLA-DRB1*12, *0901 and HLA-DQB1*05, the former might be the genetic markers for susceptibility to CU, but the latter might play a resistive role.
