MIC is one of the main problems of circulating cooling water system. The direct economic loss by MIC is about 300 to 500 billion dollars. It is good to understand MIC in order to control MIC. Source and species of mic...MIC is one of the main problems of circulating cooling water system. The direct economic loss by MIC is about 300 to 500 billion dollars. It is good to understand MIC in order to control MIC. Source and species of microorganisms was introduced firstly. There are three kinds of microorganisms in the system, including bacteria, fungi and algae. Species of these microorganisms are shown in the paper. Then, mechanisms of MIC are analysed. Although there is no universal mechanism of MIC, MIC is still mainly an electrochemical corrosion in nature. Meanwhile, the mechanisms on SRB and iron bacteria are introduced in details. At last, several methods of microorganisms control are put forward in the paper.展开更多
本实验旨在探究Mic60在小鼠卵母细胞体外成熟中的作用。选取4~6周龄的雌性C57BL/6J小鼠,注射孕马血清促性腺激素(PMSG)48 h后采集卵母细胞并随机分为对照组和Mic60抑制组(包括50、100、130、150μmol/L组,Miclxin组为130μmol/L)。卵母...本实验旨在探究Mic60在小鼠卵母细胞体外成熟中的作用。选取4~6周龄的雌性C57BL/6J小鼠,注射孕马血清促性腺激素(PMSG)48 h后采集卵母细胞并随机分为对照组和Mic60抑制组(包括50、100、130、150μmol/L组,Miclxin组为130μmol/L)。卵母细胞体外培养16 h后,通过免疫荧光染色检测纺锤体形态、ROS水平、线粒体分布和质量,利用qPCR检测抗氧化基因表达、线粒体呼吸链亚基的表达、整合应激反应相关基因的表达。结果表明:与对照组相比,130μmol/L Mic60抑制剂会降低卵母细胞体外成熟率(78.29%vs.21.08%,P<0.01),并显著阻碍纺锤体的组装;与对照组相比,Miclxin组卵母细胞中的ROS水平升高(P<0.05),且Sod1(0.83 vs. 0.22)和Sod2(0.84 vs. 0.27)基因表达降低(P<0.05),线粒体分布异常比例升高(0.16 vs. 0.57,P<0.05),线粒体膜电位水平降低(1.61 vs. 1.05,P<0.05),ATP产生降低(1.16 vs. 0.79,P<0.05);Miclxin组卵母细胞线粒体呼吸链复合体亚基Ndufv2和Sdha表达降低(P<0.05),Cyc1表达升高(P<0.05),卵母细胞mtDNA拷贝数降低(19 324 vs. 9 066,P<0.05);与对照组相比,Miclxin组卵母细胞Oma1、Dele1、Eif2ak1、eIF2α基因表达上调(P<0.05),触发了整合应激反应(Integrated Stress Response,ISR)并上调了ISR主要效应因子Atf4、Atf5和Chop的表达(P<0.05)。可见,抑制Mic60会通过损害线粒体功能并触发ISR影响卵母细胞体外成熟效果。展开更多
The first multi-color light curve analysis of the AH Mic binary system is presented.This system has very few past observations from the southern hemisphere.We extracted the minima times from the light curves based on ...The first multi-color light curve analysis of the AH Mic binary system is presented.This system has very few past observations from the southern hemisphere.We extracted the minima times from the light curves based on the Markov Chain Monte Carlo(MCMC)approach and obtained a new ephemeris.To provide modern photometric light curve solutions,we used the Physics of Eclipsing Binaries(PHOEBE)software package and the MCMC approach.Light curve solutions yielded a system temperature ratio of 0.950,and we assumed a cold starspot for the hotter star based on the O’Connell effect.This analysis reveals that AH Mic is a W-subtype W UMa contact system with a fill-out factor of 21.3%and a mass ratio of 2.32.The absolute physical parameters of the components are estimated by using the Gaia Early Data Release 3(EDR3)parallax method to be M_(h)(M_(⊙))=0.702(26),M_(c)(M_(⊙))=1.629(104),R_(h)(R_(⊙))=0.852(21),R_(c)(R_(⊙))=1.240(28),L_(h)(L_(⊙))=0.618(3)and L_(c)(L_(⊙))=1.067(7).The orbital angular momentum of the AH Mic binary system was found to be 51.866(35).The components’positions of this system are plotted in the Hertzsprung-Russell diagram.展开更多
文摘MIC is one of the main problems of circulating cooling water system. The direct economic loss by MIC is about 300 to 500 billion dollars. It is good to understand MIC in order to control MIC. Source and species of microorganisms was introduced firstly. There are three kinds of microorganisms in the system, including bacteria, fungi and algae. Species of these microorganisms are shown in the paper. Then, mechanisms of MIC are analysed. Although there is no universal mechanism of MIC, MIC is still mainly an electrochemical corrosion in nature. Meanwhile, the mechanisms on SRB and iron bacteria are introduced in details. At last, several methods of microorganisms control are put forward in the paper.
文摘本实验旨在探究Mic60在小鼠卵母细胞体外成熟中的作用。选取4~6周龄的雌性C57BL/6J小鼠,注射孕马血清促性腺激素(PMSG)48 h后采集卵母细胞并随机分为对照组和Mic60抑制组(包括50、100、130、150μmol/L组,Miclxin组为130μmol/L)。卵母细胞体外培养16 h后,通过免疫荧光染色检测纺锤体形态、ROS水平、线粒体分布和质量,利用qPCR检测抗氧化基因表达、线粒体呼吸链亚基的表达、整合应激反应相关基因的表达。结果表明:与对照组相比,130μmol/L Mic60抑制剂会降低卵母细胞体外成熟率(78.29%vs.21.08%,P<0.01),并显著阻碍纺锤体的组装;与对照组相比,Miclxin组卵母细胞中的ROS水平升高(P<0.05),且Sod1(0.83 vs. 0.22)和Sod2(0.84 vs. 0.27)基因表达降低(P<0.05),线粒体分布异常比例升高(0.16 vs. 0.57,P<0.05),线粒体膜电位水平降低(1.61 vs. 1.05,P<0.05),ATP产生降低(1.16 vs. 0.79,P<0.05);Miclxin组卵母细胞线粒体呼吸链复合体亚基Ndufv2和Sdha表达降低(P<0.05),Cyc1表达升高(P<0.05),卵母细胞mtDNA拷贝数降低(19 324 vs. 9 066,P<0.05);与对照组相比,Miclxin组卵母细胞Oma1、Dele1、Eif2ak1、eIF2α基因表达上调(P<0.05),触发了整合应激反应(Integrated Stress Response,ISR)并上调了ISR主要效应因子Atf4、Atf5和Chop的表达(P<0.05)。可见,抑制Mic60会通过损害线粒体功能并触发ISR影响卵母细胞体外成熟效果。
基金funded in part by the National Science Foundation(NSF 1517474,1909109)the National Aeronautics and Space Administration(NASA 17-ADAP17-68)。
文摘The first multi-color light curve analysis of the AH Mic binary system is presented.This system has very few past observations from the southern hemisphere.We extracted the minima times from the light curves based on the Markov Chain Monte Carlo(MCMC)approach and obtained a new ephemeris.To provide modern photometric light curve solutions,we used the Physics of Eclipsing Binaries(PHOEBE)software package and the MCMC approach.Light curve solutions yielded a system temperature ratio of 0.950,and we assumed a cold starspot for the hotter star based on the O’Connell effect.This analysis reveals that AH Mic is a W-subtype W UMa contact system with a fill-out factor of 21.3%and a mass ratio of 2.32.The absolute physical parameters of the components are estimated by using the Gaia Early Data Release 3(EDR3)parallax method to be M_(h)(M_(⊙))=0.702(26),M_(c)(M_(⊙))=1.629(104),R_(h)(R_(⊙))=0.852(21),R_(c)(R_(⊙))=1.240(28),L_(h)(L_(⊙))=0.618(3)and L_(c)(L_(⊙))=1.067(7).The orbital angular momentum of the AH Mic binary system was found to be 51.866(35).The components’positions of this system are plotted in the Hertzsprung-Russell diagram.
