Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type ...Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type calcium channels.T-type calcium channel blockers,such as pimozide and mibefradil,have been shown to prevent cerebral ischemia/reperfusion injury-induced brain injury.However,the role of Cav3.2 channels in cerebral ischemia/reperfusion injury remains unclear.Here,in vitro and in vivo models of cerebral ischemia/reperfusion injury were established using middle cerebral artery occlusion in mice and high glucose hypoxia/reoxygenation exposure in primary hippocampal neurons.The results showed that Cav3.2 expression was significantly upregulated in injured hippocampal tissue and primary hippocampal neurons.We further established a Cav3.2 gene-knockout mouse model of cerebral ischemia/reperfusion injury.Cav3.2 knockout markedly reduced infarct volume and brain water content,and alleviated neurological dysfunction after cerebral ischemia/reperfusion injury.Additionally,Cav3.2 knockout attenuated cerebral ischemia/reperfusion injury-induced oxidative stress,inflammatory response,and neuronal apoptosis.In the hippocampus of Cav3.2-knockout mice,calcineurin overexpression offset the beneficial effect of Cav3.2 knockout after cerebral ischemia/reperfusion injury.These findings suggest that the neuroprotective function of Cav3.2 knockout is mediated by calcineurin/nuclear factor of activated T cells 3 signaling.Findings from this study suggest that Cav3.2 could be a promising target for treatment of cerebral ischemia/reperfusion injury.展开更多
为充分利用气象信息综合分析处理系统第4版(Meteorology Information Comprehensive Analysis Process System 4,MICAPS 4)强大的气象数据显示和分析能力,在.Net Framework 4.5框架下,利用C#开发语言和MySQL数据库,基于MICAPS 4二次开...为充分利用气象信息综合分析处理系统第4版(Meteorology Information Comprehensive Analysis Process System 4,MICAPS 4)强大的气象数据显示和分析能力,在.Net Framework 4.5框架下,利用C#开发语言和MySQL数据库,基于MICAPS 4二次开发接口,设计研发了山西省灾害性天气历史个例库应用系统,实现了历史个例库管理、历史个例和相似历史个例检索与资料分析、历史个例训练与考试模拟等应用功能。目前,该系统已经较好地应用于业务中。系统不仅可扩展程度高、本地化应用方便,而且业务实用性较强,具有较高的应用推广价值。本文从系统设计、开发、功能以及实现方法等方面给予详细介绍。展开更多
基金supported by the Natural Science Foundation of Anhui Province of China,No.2208085Y32Scientific Research Plan Project of Anhui Province of China,No.2022AH020076the Chen Xiao-Ping Foundation for the Development of Science and Technology of Hubei Province,No.CXPJJH12000005-07-115(all to CT).
文摘Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type calcium channels.T-type calcium channel blockers,such as pimozide and mibefradil,have been shown to prevent cerebral ischemia/reperfusion injury-induced brain injury.However,the role of Cav3.2 channels in cerebral ischemia/reperfusion injury remains unclear.Here,in vitro and in vivo models of cerebral ischemia/reperfusion injury were established using middle cerebral artery occlusion in mice and high glucose hypoxia/reoxygenation exposure in primary hippocampal neurons.The results showed that Cav3.2 expression was significantly upregulated in injured hippocampal tissue and primary hippocampal neurons.We further established a Cav3.2 gene-knockout mouse model of cerebral ischemia/reperfusion injury.Cav3.2 knockout markedly reduced infarct volume and brain water content,and alleviated neurological dysfunction after cerebral ischemia/reperfusion injury.Additionally,Cav3.2 knockout attenuated cerebral ischemia/reperfusion injury-induced oxidative stress,inflammatory response,and neuronal apoptosis.In the hippocampus of Cav3.2-knockout mice,calcineurin overexpression offset the beneficial effect of Cav3.2 knockout after cerebral ischemia/reperfusion injury.These findings suggest that the neuroprotective function of Cav3.2 knockout is mediated by calcineurin/nuclear factor of activated T cells 3 signaling.Findings from this study suggest that Cav3.2 could be a promising target for treatment of cerebral ischemia/reperfusion injury.
文摘为充分利用气象信息综合分析处理系统第4版(Meteorology Information Comprehensive Analysis Process System 4,MICAPS 4)强大的气象数据显示和分析能力,在.Net Framework 4.5框架下,利用C#开发语言和MySQL数据库,基于MICAPS 4二次开发接口,设计研发了山西省灾害性天气历史个例库应用系统,实现了历史个例库管理、历史个例和相似历史个例检索与资料分析、历史个例训练与考试模拟等应用功能。目前,该系统已经较好地应用于业务中。系统不仅可扩展程度高、本地化应用方便,而且业务实用性较强,具有较高的应用推广价值。本文从系统设计、开发、功能以及实现方法等方面给予详细介绍。
