The chemopreventive effects of green tea and its polyphenols are well documented in the literature. Epidemiological studies have suggested that green tea consumption might be effective in the prevention of certain hum...The chemopreventive effects of green tea and its polyphenols are well documented in the literature. Epidemiological studies have suggested that green tea consumption might be effective in the prevention of certain human cancers. About 80% of the tea is consumed as black tea. Limited studies have been carried out to assess the usefulness of black tea as anti_carcinogen. The present set of investigations were initiated to study the anti_tumorigenic potential of aqueous black tea extract (ATE) in Swiss albino mice in \%in vivo\% animal bioassay, using 7, 12 dimethyl_benzanthracene (DMBA) as carcinogen. In the experimental group, 2% ATE was given orally as sole source of drinking water, while the control were allowed to drink normal water, \%ad lib.\% The results revealed that drinking of 2% ATE could effectively inhibit the onset of tumorigenesis, cumulative number of tumors and average number of tumors per mouse. In ATE drinking group 44% animals remained tumor free till the termination of experiment, i. e. 26 weeks. In the second set of experiment the preventive efficacy of 2% ATE of different cultivars of black tea, viz orthodox, CTC and dust were tested in Ehrlich Ascites (EA) tumor bearing mice. The preventive effects of ATE were observed in terms of increased life span (ILS). All the cultivars of tea showed more than 25% increase in life span of the animals. Cytotoxic effect of various doses of all three cultivars of black tea was also observed \%in vitro \%on EA cells.展开更多
This study determined the effect of orally fed polysaccharide-rich bioprocessed (fermented) black rice bran produced by culturing with shiitake (Lentinus edodes) mushroom mycelium on CT-26 colon cancer cells in vivo i...This study determined the effect of orally fed polysaccharide-rich bioprocessed (fermented) black rice bran produced by culturing with shiitake (Lentinus edodes) mushroom mycelium on CT-26 colon cancer cells in vivo in an intracutaneously transplanted mouse tumor alone and in combination with intraperitoneally administered anti-PD-1 immune checkpoint inhibitor. Analysis of the isolated tumor weights at the end of the study shows that the average tumor size in control mice is 3.78 grams, and the average tumor size in mice treated with anti-PD-1 antibody is 2.16 grams. The average tumor size in mice treated with BRB-F alone is 2.25 grams, and the average tumor size in mice treated with anti-PD-1 antibody BRB-F combination is 1.38 grams. Thus, BRB-F or anti-PD-1 antibody alone each reduce tumor size by 40.5% or 42.9%, whereas the combination of BRB-F and anti-PD-1 antibody reduces tumor size by 63.5%, with their cooperative effect being statistically significant. The observed anti-tumor effects were accompanied by a series of biomarkers associated with cancer formation and inhibition. These results indicate that the reported potentiation of cancer therapy using drug-based medical chemotherapies with added checkpoint inhibitors in human patients are mechanistically similar with the functional food evaluated in the present study. These beneficial effects in mice challenge clinicians to investigate if the black rice bran food product can also protect against human cancer.展开更多
文摘The chemopreventive effects of green tea and its polyphenols are well documented in the literature. Epidemiological studies have suggested that green tea consumption might be effective in the prevention of certain human cancers. About 80% of the tea is consumed as black tea. Limited studies have been carried out to assess the usefulness of black tea as anti_carcinogen. The present set of investigations were initiated to study the anti_tumorigenic potential of aqueous black tea extract (ATE) in Swiss albino mice in \%in vivo\% animal bioassay, using 7, 12 dimethyl_benzanthracene (DMBA) as carcinogen. In the experimental group, 2% ATE was given orally as sole source of drinking water, while the control were allowed to drink normal water, \%ad lib.\% The results revealed that drinking of 2% ATE could effectively inhibit the onset of tumorigenesis, cumulative number of tumors and average number of tumors per mouse. In ATE drinking group 44% animals remained tumor free till the termination of experiment, i. e. 26 weeks. In the second set of experiment the preventive efficacy of 2% ATE of different cultivars of black tea, viz orthodox, CTC and dust were tested in Ehrlich Ascites (EA) tumor bearing mice. The preventive effects of ATE were observed in terms of increased life span (ILS). All the cultivars of tea showed more than 25% increase in life span of the animals. Cytotoxic effect of various doses of all three cultivars of black tea was also observed \%in vitro \%on EA cells.
文摘目的研究辛前甘桔汤对慢性鼻窦炎(CRS)小鼠长链非编码RNA小核仁RNA宿主基因16(lnc RNA SNHG16)、表皮生长因子受体(EGFR)及黏蛋白5AC(MUC5AC)m RNA表达的影响。方法36只C57BL/6小鼠随机分为正常组、模型组、羧甲司坦组及辛前甘桔汤低、中、高剂量组,每组6只。采用金黄色葡萄球菌鼻腔灌注建立小鼠CRS模型。正常组与模型组小鼠灌胃质量分数为0.9%的氯化钠溶液(0.6 g·kg^(-1)·d^(-1)),辛前甘桔汤低、中、高剂量组分别灌胃辛前甘桔汤水煎液(0.3、0.6、1.2 g·kg^(-1)·d^(-1)),羧甲司坦组灌胃羧甲司坦口服液(0.3 m L·kg^(-1)·d^(-1)),连续干预14 d。采用苏木精-伊红(HE)及阿尔新蓝-过碘酸雪夫(AB-PAS)染色法观察小鼠鼻黏膜结构,免疫组织化学法观察小鼠鼻黏膜EGFR、MUC5AC表达并测量光密度值;酶联免疫吸附试验(ELISA)检测样本中白介素-6(IL-6)、白介素-8(IL-8)、基质金属蛋白酶-9(MMP-9)水平。实时荧光定量逆转录聚合酶链式反应(RT-q PCR)检测样本中lnc RNA SNHG16、EGFR、MUC5AC m RNA的表达。结果与正常组比较,模型组鼻黏膜上皮结构紊乱,IL-6、IL-8、MMP-9含量明显升高(P<0.05),lnc RNA SNHG16、EGFR、MUC5AC m RNA表达明显升高(P<0.05)。与模型组比较,辛前甘桔汤各剂量组IL-6水平显著降低(P<0.05),辛前甘桔汤中、高剂量组MMP-9、IL-8水平降低(P<0.05),辛前甘桔汤各剂量组lnc RNA SNHG16、EGFR、MUC5AC m RNA表达显著降低(P<0.05)。与羧甲司坦组比较,辛前甘桔汤高剂量组lnc RNA SNHG16、EGFR m RNA表达降低(P<0.05)。结论辛前甘桔汤能降低lnc RNA SNHG16、EGFR、MUC5AC m RNA表达,降低EGFR、MUC5AC蛋白表达,减少IL-6、IL-8、MMP-9表达,进而改善鼻黏膜病理改变。
文摘This study determined the effect of orally fed polysaccharide-rich bioprocessed (fermented) black rice bran produced by culturing with shiitake (Lentinus edodes) mushroom mycelium on CT-26 colon cancer cells in vivo in an intracutaneously transplanted mouse tumor alone and in combination with intraperitoneally administered anti-PD-1 immune checkpoint inhibitor. Analysis of the isolated tumor weights at the end of the study shows that the average tumor size in control mice is 3.78 grams, and the average tumor size in mice treated with anti-PD-1 antibody is 2.16 grams. The average tumor size in mice treated with BRB-F alone is 2.25 grams, and the average tumor size in mice treated with anti-PD-1 antibody BRB-F combination is 1.38 grams. Thus, BRB-F or anti-PD-1 antibody alone each reduce tumor size by 40.5% or 42.9%, whereas the combination of BRB-F and anti-PD-1 antibody reduces tumor size by 63.5%, with their cooperative effect being statistically significant. The observed anti-tumor effects were accompanied by a series of biomarkers associated with cancer formation and inhibition. These results indicate that the reported potentiation of cancer therapy using drug-based medical chemotherapies with added checkpoint inhibitors in human patients are mechanistically similar with the functional food evaluated in the present study. These beneficial effects in mice challenge clinicians to investigate if the black rice bran food product can also protect against human cancer.
