Diabetic neuropathy(DN)is,at least in part,associated with the functional attenuation of vasa nervorum,the microvascular structure of peripheral nerves.Microvascular imaging options for vasa nervorum still remain limi...Diabetic neuropathy(DN)is,at least in part,associated with the functional attenuation of vasa nervorum,the microvascular structure of peripheral nerves.Microvascular imaging options for vasa nervorum still remain limited.In this work,optical microangiography(OMAG),a volumetric,label-free imaging technique,is utilized for characterizing,with high resolution,blood perfusion of peripheral nerve in diabetic mice.We demonstrate that OMAG is able to visualize the structure of microvasculature and to quantify the changes of dynamic blood flow and vessel diameters during administration of vessel stimulant in both diabetic and normal mice.The results indicate the potential of OMAG to assess the blood supply of nerve involved in the pathology and treatment of DN.展开更多
The appearanee of blood vessels is an important biomarker to distinguish diseased from healthy tissues in several fields of medical applications. Photoacoustie microangiography has the advantage of directly visualizin...The appearanee of blood vessels is an important biomarker to distinguish diseased from healthy tissues in several fields of medical applications. Photoacoustie microangiography has the advantage of directly visualizing blood vessel networks within mierocireulatory tissue. Usually these images are interpreted qualitatively. However, a quantitative analysis is needed to better describe the characteristics of the blood vessels. This Letter addresses this problem by leveraging an efficient multiscale Hessian filter-based segmentation method, and four measure- ment parameters are acquired. The feasibility of our approach is demonstrated on experimental data and we expect the proposed method to be beneficial for several microcireulatory disease studies.展开更多
To elucidate the effect of the internal fixation plates on the local bone blood sapply, we used microangiography and scanning electron microscopy to observe the morphological changes of microcirculation in the cortica...To elucidate the effect of the internal fixation plates on the local bone blood sapply, we used microangiography and scanning electron microscopy to observe the morphological changes of microcirculation in the cortical bones obtained from intact rabbit tibiae on which plates of two different stiffness had been fixed for comparison. The results indicated that both rigid stainless steel plate and less rigid methyl methacrylate plate could induce the bone microcirculation under the plate to undergo a process from early depression to late reactive recruitment. The features of the microcircuiation recruitment such as vascular number, arrangement and dilatation varied with plates of different stiffness and were more obvious in the cortex fixed by rigid stainless steel plate.展开更多
基金supported in part by research grants from the National Institutes of Health(R01HL093140,R01EB009682,and R01DC010201)(RKW)the American Heart Association(0855733G)(RKW)the National Institute of Diabetes and Digestive and Kidney Diseases(5 U24 DK076169-04,Pilot and Feasibility Study 09MCG81)(TKB).
文摘Diabetic neuropathy(DN)is,at least in part,associated with the functional attenuation of vasa nervorum,the microvascular structure of peripheral nerves.Microvascular imaging options for vasa nervorum still remain limited.In this work,optical microangiography(OMAG),a volumetric,label-free imaging technique,is utilized for characterizing,with high resolution,blood perfusion of peripheral nerve in diabetic mice.We demonstrate that OMAG is able to visualize the structure of microvasculature and to quantify the changes of dynamic blood flow and vessel diameters during administration of vessel stimulant in both diabetic and normal mice.The results indicate the potential of OMAG to assess the blood supply of nerve involved in the pathology and treatment of DN.
基金supported by the National Natural Science Foundation of China(Nos.61201307,61371045,and 61171197)the Fundamental Research Funds for the Central Universities(No.HIT.NSRIF.2013132)
文摘The appearanee of blood vessels is an important biomarker to distinguish diseased from healthy tissues in several fields of medical applications. Photoacoustie microangiography has the advantage of directly visualizing blood vessel networks within mierocireulatory tissue. Usually these images are interpreted qualitatively. However, a quantitative analysis is needed to better describe the characteristics of the blood vessels. This Letter addresses this problem by leveraging an efficient multiscale Hessian filter-based segmentation method, and four measure- ment parameters are acquired. The feasibility of our approach is demonstrated on experimental data and we expect the proposed method to be beneficial for several microcireulatory disease studies.
文摘To elucidate the effect of the internal fixation plates on the local bone blood sapply, we used microangiography and scanning electron microscopy to observe the morphological changes of microcirculation in the cortical bones obtained from intact rabbit tibiae on which plates of two different stiffness had been fixed for comparison. The results indicated that both rigid stainless steel plate and less rigid methyl methacrylate plate could induce the bone microcirculation under the plate to undergo a process from early depression to late reactive recruitment. The features of the microcircuiation recruitment such as vascular number, arrangement and dilatation varied with plates of different stiffness and were more obvious in the cortex fixed by rigid stainless steel plate.
