Effects of Hormone Factors on the in vitro Culture Flowering Induction of Dendrobium officinate Kimura et Migo

[Objective]The aim was to select the suitable hormone factors for flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.[Method]The test-tube plantlets from the stems of Dendrobium officinate Kimura et Migo were used as the experimental materials and MS medium as the basic medium.Comparative tests have been done between single-factor hormone treatments（different concentrations of PP333 or TDZ） and multi-factor hormone treatments（different combinations of PP333,TDZ,6-BA and NAA） to research the effects of hormone factors on the flowering induction of the plantlets.[Result]Among the single-factor hormone treatments,the suitable concentration and the rate of flower buds formation of PP333 treatment were 0.2 mg/L and 8.5%,the that of TDZ treatment were 0.06 mg/L and 15.5%;the effects of multi-factor hormone treatments on the flowering induction were ordered as follow：（PP333 ＋ 6-BA ＋ NAA ＋ TDZ）〉 （PP333 ＋ 6-BA ＋ NAA）〉 （PP333 ＋ 6-BA） and（PP333 ＋ NAA） ;the most suitable treatment was PP333 0.3 mg/L ＋ 6-BA 0.5 mg/L ＋ NAA 0.5 mg/L ＋ TDZ 0.06 mg/L,the rate of flower bud formation and the rate of the blossomed flower were respectly reached to 80.4% and 90.3%.[Conclusion]PP333 and TDZ showed the important effect on the flowering induction in vitro culture of Dendrobium officinate Kimura et Migo.The effect of TDZ was better than that of PP333.It is much more conducive to the flower bud formation,when using appropriate concentration of TDZ combined with other hormones properly.

Two New Isosteroid Alkaloids from Fritillaria monatha Migo（Ⅲ）

Two new isosteroid alkaloids, named pengbeimine A and pengbeimine C, were isolated from the fresh bulbs of FritiUaria monatha Migo. Their structures were determined by spectral methods, and were proved by X-ray diffraction as well.

Functions of Plant Growth Substances in the Growth of Dendrobium officinale Kimura et Migo

[Objectives] To study the effects of plant growth substance on the growth and development of Dendrobium officinale Kimura et Migo. [Methods] Comparative methods were applied to study the effects of plant growth substance on the growth and development of D. officinale Kimura et Migo. [Results] Plant growth regulators play an important role in the process of tissue culture rapid propagation of D. officinale Kimura et Migo,and the main regulatory pathways are as follows.( i) Plant growth regulators promote the growth and development of D. officinale Kimura et Migo through regulating the changes of its endogenous hormones.( ii) Plant growth regulators affect the accumulation of polysaccharide content in D. officinale Kimura et Migo through regulating the changes in chlorophyll content and enzymes involved in sucrose metabolism.( iii) Plant growth regulators can increase the activity of antioxidant enzymes of D. officinale Kimura et Migo,to increase its stress resistance. [Conclusions]Using molecular biology techniques,combined with the changes of plant hormones in D. officinale Kimura et Migo,and analysis on key enzyme in the synthesis of plant hormones and gene expression,it is feasible to study the rules o changes in the content of active content of D. officinale Kimura et Migo.

Efficacy Evaluation of 16% Validamycin·thifluzamide SC against Selerotium rolfsii in Dendrobium officinale Kimura et Migo

[Objective] The paper was to understand the control efficacy and the optimal dosage of 16% validamycin？thifluzamide SC on controlling Selerotium rolfsii in Dendrobium officinale Kimura et Migo. [Method] The control efficacy of 16% validamycin·thifluzamide SC against S.rolfsii was determined by spraying agents on the whole plant of D.officinale. The significance of differences was analyzed by Duncan＇s new multiple range test （DMRT）. [Result] The control efficacy of 16% validamycin.thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg was 85.33% at 14 d post spraying, higher than that of control agent 4% validamycin AS sprayed at the dose of 80 mg a.i./kg （78.36%）, and equivalent to that of control agent 240 g/L thifluzamide SC sprayed at the dose of 120 mg a.i./kg （90.60%）. [Conclusion] 16% Validamycin·thifluzamide SC sprayed at the doses of 80-160 mg a.i./kg could effectively control the damage of S.rolfsii in D.officinale, and could be popularized in the production.

Research Progress of Dendrobium officinale Kimura et Migo

The effects of light on the quality and related genes of Dendrobium officinale were summarized, and the main chemical components and pharmacological effects of D. officinale were analyzed. The biomass accumulation of D. officinale is related to light quality and light intensity. Monochromatic light quality can make polysaccharide accumulate, and mixed light quality can promote cell proliferation and component accumulation of D. officinale. Under different light intensity, the polysaccharide content of D. officinale is different, and it changes with the change of light intensity. When a certain intensity is exceeded, the polysaccharide content decreases with the increase of light intensity. The chemical components of D. officinale are polysaccharides, alkaloids, amino acids, volatile components, etc. The analysis of the growth and components of D. officinale provides reference and basis for improving the quality and chemical components of D. officinale and promotes the research and development of related products of D. officinale.

New Utilization Model of Karst Rocky Desertified Land in Southwest China:Ecological Planting of Dendrobium officinale Kimura et Migo on Bare Rocky Mountains

The Yunnan-Guangxi-Guizhou rocky desertified area in southwestern China is one of the contiguous extremely poor areas identified in the Outline of Poverty Alleviation and Development in the Rural Areas of China(2011-2020).In rocky desertified areas,due to long-term severe soil erosion,large areas of bedrock are exposed or gravels are accumulated.This bare rock and gravel-mulched land has become the main land type in the rocky mountains.Under normal circumstances,it cannot be directly used for agriculture,forestry,animal husbandry,and fishery production,and is classified as land that is difficult to be utilized.In recent years,in Debao County,Baise City,Guangxi Zhuang Autonomous Region,as a deeply impoverished county in the Yunnan-Guangxi-Guizhou rocky desertified area,a new land use model of karst rocky desertified land,that is,planting Dendrobium officinale Kimura et Migo on bare rocky ground,has emerged,and certain poverty alleviation benefits have been achieved initially.In this article,on the basis of analyzing the suitability of planting D.officinale Kimura et Migo on karst rocky desertified land,the practice of planning D.officinale Kimura et Migo on the rocky desertified land in Debao County was elucidated,and then suggestions for reasonable promotion of ecological planting of D.officinale Kimura et Migo in karst rocky desertified areas were put forward.

Study on Mechanism of Dendrobium officinale Kimura et Migo in Preventing and Treating Exercise-induced Muscle Damage(EIMD)in Rats

[Objectives]This study was conducted to observe the mechanism of Dendrobium officinale Kimura et Migo on gastrocnemius muscle in rats with exercise-induced muscle damage(EIMD).[Methods]The micro-injury model of skeletal muscle was established by treadmill training.Forty two SD rats were randomly divided into a control group,1,12 and 24 h exercise groups,D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group,and D.officinale 2 ml+24 h exercise group,with 6 rats in each group.Various D.officinale groups were given the drug once in the morning and once in the evening at a dose of 2 ml/time,a week in advance.Except for the quiet group,the samples were collected from the 1,12 and 24 h exercise groups after anesthesia following 1,12 and 24 h of exercise for the last time,respectively,and the D.officinale 2 ml+1 h exercise group,D.officinale 2 ml+12 h exercise group and D.officinale 2 ml+24 h exercise group were also sampled after anesthesia following 1,12 and 24 h of exercise for the last time,respectively.The contents of ATP,CK-MM and CK in rat serum were determined by enzyme-linked immunosorbent assay(ELISA).The histopathological changes of gastrocnemius muscle were observed by HE staining.PCR and Western-blot detection were carried out to analyze the effects of D.officinale on IGF-1 mRNA and protein expression in gastrocnemius muscle.[Results]Compared with the quiet group,the ATP contents in the serum of rats in the 1,12 and 24 h exercise groups significantly decreased(P<0.01),while the CK and CK-MM contents significantly increased(P<0.01).The expression of IGF-1 mRNA and protein in the gastrocnemius muscle tissue significantly increased(P<0.01).Compared with the 1 h exercise group,the ATP content and IGF-1 protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+1 h exercise group significantly increased(P<0.05),while the CK and CK-MM contents significantly decreased(P<0.01).Compared with the 12 h exercise group,the D.officinale liquid+12 h exercise group showed a significant increase in ATP content(P<0.01),significant increases in IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue(P<0.01),and significant decreases in CK and CK-MM contents(P<0.01).Compared with the 24 h exercise group,the ATP content and IGF-1 mRNA and protein expression in the gastrocnemius muscle tissue of the D.officinale liquid+24 h exercise group significantly increased(P<0.01),while the CK and CK-MM contents significantly decreased(P<0.01).From the pathological tissue morphology of the gastrocnemius muscle in rats with EIMD treated with D.officinale,it could be concluded that the gastrocnemius muscle of each exercise group was significantly damaged,and the damage was significantly alleviated after administration of D.officinale liquid.[Conclusions]The effects and mechanism of D.officinale on prevention and treatment of EIMD in rats might be related to the promotion of IGF-1 mRNA and protein expression in injured tissues by reducing ATP energy consumption,CK-MM and CK activity.

MIGO秘书综合事务所微信公众号运营现状、问题及对策

微信作为当下主流的新媒体平台,可实现与特定群体进行文字、图片、语音和视频的全方位沟通。目前,微信公众号已广泛应用到高校专业教学领域,创新了人才培养模式。MIGO秘书综合事务所微信公众号是广东海洋大学秘书学专业自主运营的一个微信公众号平台,在技能大赛动态追踪、关注专业发展动态、考研经验分享上具有一定的影响力,但也存在技能学习栏目缺乏,未能有效挖掘平台资源,校园服务功能单一的问题。对此,可通过增加技能学习栏目,契合用户需求;与第三方平台合作,增设校园服务功能;增强用户黏度,实现资源共享,来提高该微信公众号的服务能力,也为此类微信公众号的运营提供参考。

Clinical Effect of Dendrobium officinale Kimura et Migo from Longhushan on Treatment of Type 2 Diabetes

[Objectives] To observe and compare the clinical effect and safety of Dendrobium officinale Kimura et Migo from Longhushan on treatment of type 2 diabetes. [Methods] 90 patients with type 2 diabetes were randomly divided into group A,group B and control group by randomized control method. Group A adopted D. officinale Kimura et Migo and metformin hydrochloride; group B adopted D. officinale Kimura et Migo; the control group adopted metformin hydrochloride. The fasting plasma glucose( FPG) of 3 groups was measured before the treatment and 2 weeks,4 weeks,6 weeks,8 weeks,10 weeks,and 12 weeks after the treatment,Hb A1 c was measured and diabetes symptom grade score was counted before the treatment and 12 weeks after treatment,observation indicators and clinical effect of 3 groups of patients were compared. [Results] The fasting plasma glucose level in group A was lower than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P >0. 05); Hb A1 c of group A for 12 weeks after treatment was lower than that of other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05); the difference in diabetes symptom grade score of group A was greater than that in other 2 groups,the difference was statistically significant( P < 0. 01),and there was no statistical significance in the difference between group B and the control group( P > 0. 05). [Conclusions]D. officinale Kimura et Migo from Longhushan can effectively reduce the fasting plasma glucose level and Hb A1 c of type 2 diabetic patients and effectively improve the symptoms of patients; clinical application of D. officinale Kimura et Migo has equivalent effect with metformin hydrochloride,and combination of D. officinale Kimura et Migo with metformin hydrochloride has better effect than single use.

铁皮石斛苗床下立体栽培羊肚菌技术及其对土壤理化性质的影响

探讨四川省攀枝花市秋末冬初设施塑料大棚中羊肚菌(Morchella spp.)-铁皮石斛(Dendrobium officinale Kimura et Migo)立体栽培的可行性,测定在羊肚菌生长过程中的菌丝生长期(M1)、子实体生长期(M2)和采收期(M3)表层土壤的pH、全氮、铵态氮、速效磷、有效钾、有机质、电导率(Electric conductivity,EC)和固体溶解性物质总量(Total dissolved solids,TDS)等。结果表明,与对照组相比,在羊肚菌生长的过程中,试验组不同时期表层土壤的pH和有效钾含量均下降,而速效磷、全氮、铵态氮、EC、TDS和有机质的含量均显著增高,且产量达(1950±180)kg/hm^(2)。

中药石斛研究的回顾与展望

中药石斛基原复杂,其主流品种有霍山石斛Dendrobium huoshanense C.Z.Tang et S.J.Cheng、铁皮石斛D.officinale Kimura et Migo、金钗石斛D.nobile Lindl.,《中华人民共和国药典》(以下简称《中国药典》)2005年版石斛项下增加了“及其近似种”的描述,难以确定具体物种,有必要对其进一步正本清源。对石斛属的起源进行本草考证,对霍山石斛、铁皮石斛、金钗石斛的本草起源进行梳理,为不同石斛属药材的应用提供参考。霍山石斛是最早作为中药石斛应用的品种,高效液相色谱法特征图谱等方法为《中国药典》2020年版收录霍山石斛提供了技术支撑;铁皮石斛应为石斛药材中应用时间最久、范围最广的品种,其主要为浙江、云南、贵州,以及广东、广西、福建、江西等地丹霞地貌上生长的种群,应高度重视并对其进行保护;D.nobile在历代文献中的中文名称混乱,应明确将其作为金钗石斛的拉丁学名。枫斗是我国名贵中药和传统保健品,起源于霍山石斛。古代名医对霍山石斛、铁皮石斛、金钗石斛、川石斛的用法各有特色,建议厘清品种来源、功效,加强现代研究,在修订《中国药典》时将其各自单列,充分发挥中药石斛的临床价值。

缺素及缺素恢复对铁皮石斛组培苗的影响

为探究12种必需矿质元素(包括N、P、K、Ca、Mg、S、Fe、Mn、Cu、Zn、B、Mo)对铁皮石斛(Dendrobium officinale Kimura et Migo)生长及生理指标的影响,以铁皮石斛3个月组培苗为材料,以MS培养基为基础设置全营养对照(CK)、单一缺素处理和缺素恢复处理,分析各处理条件下铁皮石斛的表型特征和生理变化。结果显示:缺N、P、Ca、Cu、Zn处理组铁皮石斛组培苗缺素症状明显,整株的生长状态及叶片和假鳞茎的解剖结构均受到影响;除Zn外,其他元素缺素恢复处理后症状均得到明显改善。缺N、K、Ca、S、Zn处理组假鳞茎长及缺N、Ca处理组的叶片数受抑制明显。除P外,其他元素缺素处理组叶片叶绿素含量明显降低,多数缺素恢复处理组叶片叶绿素a和叶绿素b含量较缺素处理组极显著(P<0.01)或显著(P<0.05)升高;缺Fe处理组的叶绿素a含量与叶绿素b含量的比值明显降低,恢复处理后接近于CK。除Fe外,其他微量元素缺素处理对叶片超氧化物歧化酶(SOD)活性抑制明显,而缺大量元素处理组的SOD活性均不同程度升高;缺P处理组的过氧化物酶(POD)活性明显降低,而缺N、Mg、S、Mn、Zn、Cu处理组的POD活性明显升高,除Mn外,其他元素缺素恢复处理组的POD活性均升高。缺N、P可大幅提高叶片可溶性糖含量,缺N、Mn、Cu可大幅提高叶片可溶性蛋白质含量。缺N、Cu处理组假鳞茎多糖含量大幅高于CK,Ca、Mo缺素恢复处理组假鳞茎多糖含量较缺素处理组极显著升高;缺Mg处理组假鳞茎总黄酮含量大幅高于CK。综上,在保证铁皮石斛正常生长的前提下,可通过Ca、Mo缺素恢复处理来提高铁皮石斛假鳞茎多糖含量,通过适量缺Mg处理来提高铁皮石斛假鳞茎总黄酮含量。

铁皮石斛miR168分子进化特征及响应蓝激光表达分析

笔者在关于铁皮石斛miRNA组学研究中发现较多响应蓝激光的miRNA,挑选关键且差异表达的miR168进行分子进化特征及其响应蓝激光表达分析。经对前体和成熟体分类统计、进化树构建、靶基因预测、启动子顺示作用元件及响应不同光质表达等进行分析,结果显示:miR168家族成员分布在40个植物物种中,前体和成熟体成员较少,其在植物中可能进化得不够完整;物种特异性是影响miR168前体进化特性的重要因素,而序列保守性是影响成熟体进化特性的主要因素;由3p臂上形成的miR168成熟体序列有较高特异性、5p臂形成的成熟体的序列保守性较高;Mfold和Rfam预测表明铁皮石斛miR168茎序列的保守性高于环序列,前体发卡结构较多,成熟体位置位于3p臂上,既包含茎序列也包含环序列;铁皮石斛miR168a的启动子主要包括光、激素、生物和非生物胁迫响应元件,可能通过这些顺式元件在其响应蓝激光中起调控作用;铁皮石斛响应蓝激光的靶基因主要包括ABC转运蛋白B家族成员、乙酰辅酶A乙酰转移酶等,主要参与植物生长发育和响应外界因素。实时荧光定量PCR结果表明,铁皮石斛miR168a与其靶基因gene-MA16_Dca006149、gene-MA16_Dca020995、gene-MA16_Dca017821参与蓝激光对铁皮石斛的影响。

韶关丹霞地貌铁皮石斛野生资源调查与生境分析

目的:调查广东韶关丹霞地貌现存铁皮石斛野生资源的生存现状,为野生铁皮石斛就地保护、回归保育及仿野生栽培提供参考。方法:对国内铁皮石斛野生资源情况进行整理,基于本课题组近10年来对广东韶关丹霞地貌野生铁皮石斛资源分布和生境开展的跟踪调查,对11个调查地点的海拔、朝向、铁皮石斛数量、坡位、坡向进行分析,探讨丹霞地貌野生铁皮石斛植物形态和生境特点。结果:调查记录了广东韶关丹霞地貌中的野生铁皮石斛300余丛,约3000 g,处于极度濒危状态;实地观察发现,野生铁皮石斛茎多呈紫红色,长5~10 cm,有“形似蚱蜢髀”的茎条,叶铁锈色斑点明显;丹霞地貌的野生铁皮石斛大多生长在海拔100~500 m的丹霞崖壁中部或近坡顶处,常与苔藓和地衣伴生,一般生长在东南至西南朝向、通风良好、傍水潮湿的丹霞岩壁上,且半阳偏阴、温凉高湿环境下长势较好。结论:野外考察证实,《本草经集注》中记载的“今用石斛出始兴”的石斛为铁皮石斛,其药用历史在1500年以上;丹霞地貌的铁皮石斛为目前国内铁皮石斛的主要种源之一;野生丹霞铁皮石斛因自身繁殖率低、人为长期采摘等原因,现已极度濒危,亟须开展铁皮石斛野生资源的调查与保护工作。

乐清市铁皮石斛总黄酮含量的测定

[目的]测定乐清市铁皮石斛总黄酮的含量。[方法]以芦丁为对照品,采用NaNO2-Al(NO3)3-NaOH显色,在510 nm波长处用紫外分光光度法测定总黄酮的含量。[结果]芦丁在0.011 1～0.066 7 mg/ml浓度范围内与吸光度呈良好线性关系(R2=0.999 2);样品溶液在12 h之内稳定性良好,RSD值为0.15%;建立的方法重复性良好,其RSD值为3.12%。[结论]该方法简便、准确、稳定,可用于铁皮石斛中总黄酮含量的测定。

基于品质指标的正交试验优化铁皮石斛真空冷冻干燥工艺

【目的】在现有工业生产的铁皮石斛真空冷冻干燥工艺基础上优化时间参数,以期获得最佳干燥工艺,提高效率同时提升产品品质,为铁皮石斛干制产品工业化生产提供参考依据。【方法】以新鲜铁皮石斛为原料,采用正交试验考察不同升华时间对铁皮石斛多糖、甘露糖、醇浸出物和多酚含量及抗氧化活性的影响,与现有工业生产的真空冷冻干燥工艺进行对比,确定最佳真空冷冻干燥工艺。【结果】各因素对真空冷冻干燥铁皮石斛品质的影响主次顺序:35℃所用时间>55℃所用时间>25℃所用时间>45℃所用时间;铁皮石斛的最佳真空冷冻干燥工艺参数:真空度30Pa,冷阱温度-40℃,升华温度和升华时间为15℃(3 h)→25℃(3 h)→35℃(5 h)→45℃(9 h)→55℃(18 h),共38h,在此条件下,铁皮石斛多糖含量为35.3%,甘露糖含量为23.1%,醇浸出物含量为7.8%,1,1-二苯基-2-苦基肼(DPPH)自由基清除率为30.3%,综合评分为88.3分。与现有真空冷冻干燥铁皮石斛工艺相比,干燥时间缩短2.6%,多糖和甘露糖含量分别显著提高27.0%和5.0%(P<0.05,下同),醇浸出物含量无显著差异(P>0.05),DPPH自由基清除率显著减少31.0%,多酚含量相当,色泽较接近新鲜的铁皮石斛样品。【结论】经优化后真空冷冻干燥工艺得到的铁皮石斛有效成分含量较高,干燥时间缩短,色泽更佳。

贵州栽培种铁皮石斛遗传多样性分析

目的研究贵州栽培种铁皮石斛的遗传多样性,可为种质资源利用及遗传改良提供参考。方法利用简单重复序列间区(Inter-simple sequence repeats,ISSR)、简单重复序列(simple sequence repeat,SSR)分子标记技术,对贵州20份分别来自浙江、云南的栽培种的铁皮石斛进行遗传多样性研究,使用NTsys、Popgen32等软件进行遗传分析。结果经筛选得到32对多态性引物,共扩增得到152条清晰的条带,平均每对引物扩增4.75条带,其中多态性条带145条,平均每对引物扩增4.53条多态性条带,其条带的多态率(PPL)为96.05%。经遗传分析,Nei’s基因多样性指数(H)平均值为0.3507,Shannon’s多态性信息指数(I)平均值为0.5219等位基因数(Ne)平均值为1.5992、观测等位基因数(Na)平均值为1.9605,这表明研究所用铁皮石斛具有丰富的遗传变异。聚类分析结果表明,根据来源可将20个贵州栽培种铁皮石斛聚为2类。结论ISSR及SSR分子标记可以有效应用于铁皮石斛遗传多样性分析中,贵州铁皮石斛遗传变异丰富,应加强不同来源的铁皮石斛在系统选育及遗传改良中的合理利用。

石斛抗氧化和滋阴作用的试验研究

[目的]研究金钗石斛、鼓槌石斛和铁皮石斛的抗氧化和滋阴药效异同,为其临床用药提供一定的科学依据。[方法]采用DPPH.清除率检测法评价其体外抗氧化活性;并采用氢化可的松建立阴虚大鼠模型,用试剂盒法测定血清中的超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-PX)和乳酸脱氢酶(LDH)的活力,并计算大鼠体重、进食量以及死亡率,评价石斛滋阴效果。[结果]体外抗氧化试验表明,3种石斛的抗氧化作用强弱为:铁皮石斛>鼓槌石斛>金钗石斛,铁皮石斛与鼓槌石斛抗氧化活性和Vc接近,金钗石斛次之;浙江产铁皮石斛和云南产鼓槌石斛抗氧化效果相当,均优于其他产地的。滋阴试验结果表明,铁皮石斛和鼓槌石斛滋阴作用等同,金钗石斛次之。[结论]铁皮石斛、金钗石斛和鼓槌石斛抗氧化和滋阴有差异,在临床使用时可有选择的使用。

铁皮石斛栽培技术概况

铁皮石斛是我国传统中药材,属于国家二类保护药用植物。铁皮石斛植物体内含有丰富的多糖和甘露糖,对人们的健康具有独特的保健与药用价值。文章系统地简述了铁皮石斛的总体发展概况、产业政策、品种选择及种苗组织培养生产,重点讨论了栽培技术要点和种植基地的区域性和安全性以及发展前景和存在问题,并探讨了品种选育、栽培模式、施肥技术、病虫害等因素对铁皮石斛药用价值的影响。结果显示,扩展分布范围并保证种植基地的安全可以提高药材的质量;栽培品种的选育对药材质量也有一定的影响;试管苗工业化生产能够快速的培育出质量优良的品种;光照、基质、施肥、采收等因素都在一定程度上影响栽培品的质量。因此,根据这些理论基础,设计出一整套高效、安全的铁皮石斛种植栽培的模式,可以提高铁皮石斛的品质,并促进整个行业的可持续发展。

镧对铁皮石斛幼苗生理特性及品质的影响

为了研究稀土镧（La^3＋）对铁皮石斛（Dendrobium officinale Kimura et Migo）幼苗生理特性和品质的影响,在温室里用5-120 mg/L的镧溶液分别对铁皮石斛幼苗进行了喷施试验。结果显示,随着镧溶液浓度的升高,幼苗叶片和茎秆中叶绿素、可溶性蛋白、脯氨酸、多糖、石斛碱的含量呈先增加、后降低的变化趋势,而丙二醛的含量先降低、后增加。综合而言,用20 mg/L镧溶液浓度喷施铁皮石斛幼苗为最佳处理浓度,此时叶片中叶绿素的含量比空白对照增加54.37%、可溶性蛋白含量增加53.64%、脯氨酸含量增加99.20%、丙二醛含量降低44.71%、多糖含量增加56.60%、石斛碱含量增加14.06%;茎秆中叶绿素的含量比空白对照增加17.48%、可溶性蛋白含量增加56.57%、脯氨酸含量增加253.64%、丙二醛含量降低74.91%、多糖含量增加88.73%、石斛碱含量增加24.54%。表明20 mg/L的镧溶液浓度处理能有效的促进铁皮石斛的光合作用、增强抗逆性和提高品质。