Protein kinase inhibitor 6-DMAP was used to explore the effect of protein phosphorylation on germinal vesicle breakdown(GVBD) and chromatin/chromosome behavior of mousc oocytes at different stages .Protein phosphoryl...Protein kinase inhibitor 6-DMAP was used to explore the effect of protein phosphorylation on germinal vesicle breakdown(GVBD) and chromatin/chromosome behavior of mousc oocytes at different stages .Protein phosphorylation induced by 6-DMAP-sensitive protein kinases was not required for GVBD 2 hrs after culture. 6-DMAP stimulated transition to interphase in oocytes after GVBD and inhibited extrusion of the first polar body(PB1) and the second polar body(PB2). This drug also induced the reformation of nucleus in metaphase II(M II) oocytes spontaneously,without the release of cortical granules(CGs), a result that is different from the conclusion obtained before.展开更多
文摘Protein kinase inhibitor 6-DMAP was used to explore the effect of protein phosphorylation on germinal vesicle breakdown(GVBD) and chromatin/chromosome behavior of mousc oocytes at different stages .Protein phosphorylation induced by 6-DMAP-sensitive protein kinases was not required for GVBD 2 hrs after culture. 6-DMAP stimulated transition to interphase in oocytes after GVBD and inhibited extrusion of the first polar body(PB1) and the second polar body(PB2). This drug also induced the reformation of nucleus in metaphase II(M II) oocytes spontaneously,without the release of cortical granules(CGs), a result that is different from the conclusion obtained before.
