Extracellular vesicles(EVs)such as microvesicles(MIVs)play an important role in intercellular communications.MIVs are small membrane vesicles sized 100e1000 nm in diameter that are released by many types of cells,such...Extracellular vesicles(EVs)such as microvesicles(MIVs)play an important role in intercellular communications.MIVs are small membrane vesicles sized 100e1000 nm in diameter that are released by many types of cells,such as mesenchymal stem cells(MSCs),tumor cells and adipose-derived stem cells(ADSC).As EVs can carry out autocrine and paracrine functions by controlling multiple cell processes,it is conceivable that EVs can be used as delivery vehicles for treating several clinical conditions,such as to improve cardiac angiogenesis after myocardial infarction(MI).Here,we seek to investigate whether ADSC-derived MIVs contain microRNAs that regulate angiogenesis and affect cell migration of endothelial cells.We first characterized the ADSC-derived MIVs and found that the MIVs had a size range of 100 e300 nm,and expressed the MIV marker protein Alix.We then analyzed the microRNAs in ADSCs and ADSC-derived MIVs and demonstrated that ADSC-derived MIVs selectively released a panel of microRNAs,several of which were related to angiogenesis,including two members of the let-7 family.Furthermore,we demonstrated that ADSC-derived MIVs promoted the cell migration and invasion of the HUVEC endothelial cells.The PKH26-labeled ADSC-derived MIVs were effectively uptaken into the cytoplasm of HUVEC cells.Collectively,our results demonstrate that the ADSC-derived MIVs can promote migration and invasion abilities of endothelial cells,suggesting pro-angiogenetic potential.Future studies should focus on investigating the roles and mechanisms through which ADSC-derived MIVs regulate angiogenesis.展开更多
A computational and test method for calibrating the flight loads carried by aircraft wings is proposed.The wing load is measured in real-time based on the resistance and fiber Bragg grating strain gauges.The linear st...A computational and test method for calibrating the flight loads carried by aircraft wings is proposed.The wing load is measured in real-time based on the resistance and fiber Bragg grating strain gauges.The linear stepwise regression method is used to construct the load equations.The mean impact value algorithm is employed to select suitable bridges.In the ground calibration experiment,the wing load calculation equations in both forward and reverse installation states are calibrated.The correctness of the load equations was verified through equation error and inspection error analysis.Finally,the actual flight load of the wing was obtained through flight tests.展开更多
基金The reported work was supported in part by research grants from the Natural Science Foundation of Jiangxi Province China(#20151BAB215005)the Natural Science Foundation of China(#81660029,81360083)+2 种基金TCH was also supported by the Mabel Green Myers Research Endowment Fund,USA and The University of Chicago Orthopaedics Alumni Fund,USA.Funding sources were not involved in the study designin the collection,analysis and interpretation of data,in the writing of the reportand in the decision to submit the paper for publication.
文摘Extracellular vesicles(EVs)such as microvesicles(MIVs)play an important role in intercellular communications.MIVs are small membrane vesicles sized 100e1000 nm in diameter that are released by many types of cells,such as mesenchymal stem cells(MSCs),tumor cells and adipose-derived stem cells(ADSC).As EVs can carry out autocrine and paracrine functions by controlling multiple cell processes,it is conceivable that EVs can be used as delivery vehicles for treating several clinical conditions,such as to improve cardiac angiogenesis after myocardial infarction(MI).Here,we seek to investigate whether ADSC-derived MIVs contain microRNAs that regulate angiogenesis and affect cell migration of endothelial cells.We first characterized the ADSC-derived MIVs and found that the MIVs had a size range of 100 e300 nm,and expressed the MIV marker protein Alix.We then analyzed the microRNAs in ADSCs and ADSC-derived MIVs and demonstrated that ADSC-derived MIVs selectively released a panel of microRNAs,several of which were related to angiogenesis,including two members of the let-7 family.Furthermore,we demonstrated that ADSC-derived MIVs promoted the cell migration and invasion of the HUVEC endothelial cells.The PKH26-labeled ADSC-derived MIVs were effectively uptaken into the cytoplasm of HUVEC cells.Collectively,our results demonstrate that the ADSC-derived MIVs can promote migration and invasion abilities of endothelial cells,suggesting pro-angiogenetic potential.Future studies should focus on investigating the roles and mechanisms through which ADSC-derived MIVs regulate angiogenesis.
基金supported by the National Natural Science Foundation of China(Grant No.11602237)the Middleaged and Young Teachers’Basic Ability Promotion Project of Guangxi(Grant No.2022KY1070)。
文摘A computational and test method for calibrating the flight loads carried by aircraft wings is proposed.The wing load is measured in real-time based on the resistance and fiber Bragg grating strain gauges.The linear stepwise regression method is used to construct the load equations.The mean impact value algorithm is employed to select suitable bridges.In the ground calibration experiment,the wing load calculation equations in both forward and reverse installation states are calibrated.The correctness of the load equations was verified through equation error and inspection error analysis.Finally,the actual flight load of the wing was obtained through flight tests.