Effect of Majie Pingchuan cataplasm on epithelial-derived cytokines in asthmatic mice based on the"lung-skin-intestine"axis

Objective:To observe the effect of MJPC cataplasm on the content of epithelial-derived cytokines in lung,skin and intestine of asthmatic mice.Methods:C57/BL6 female mice were randomly divided into four groups:control group,asthma model group,dexamethasone group and MJPC cataplasm group.Ovalbumin sensitized and challenged asthmatic mouse models were established.The spleen index was calculated,and HE staining was used to observe the pathological change in lung tissues.The ova-specific IgE in the mouse serum and the content of TSLP in lung,skin and intestine were detected by enzyme-linked immunosorbent assay(ELISA).The expressions of TSLP mRNA and IL-33 mRNA in skin and intestinal tissue were detected by qRT-PCR.Results:Compared with the control group,the spleen index of mice in asthma model group was increased.Vascular congestion and edema,inflammatory cell infiltration and bronchial wall thickening were observed.The expressions of IgE in the mouse serum were significantly increased,and the content of TSLP in lung and skin tissue increased,but that in intestine tissue did not change significantly.The expression of TSLP mRNA was up-regulated in skin and intestinal tissues.The expression of IL-33 mRNA was up-regulated in skin tissue,but not in intestine,and the differences were statistically significant(P<0.05);Compared with the model group,MJPC cataplasm could decrease the spleen index and the expression of IgE in the mouse serum,improve the pathological damage of lung tissue in asthmatic mice,reduce the content of TSLP in lung,skin and intestinal tissue,increased the expression of TSLP mRNA in skin tissue,and down-regulate the expression of Il-33 mRNA in skin tissue and the expression of TSLP mRNA and IL-33 mRNA expression in intestinal tissue(P<0.05).Pearson correlation coefficient(r)of the content of TSLP between lung and skin was 0.689,that between lung and intestinal was-0.163,and that between skin and intestinal was-0.163,and the differences were not statistically significant(P>0.05).Conclusion:MJPC cataplasm improve airway inflammation by inhibiting the content of epithelial-derived cytokines on the"lung-skin-intestine"axis of asthmatic mice,and achieve the effect of treating asthm.

基于“肺⁃皮⁃肠”轴研究麻芥巴布膏对哮喘小鼠上皮源性细胞因子的影响

目的:通过麻芥巴布膏干预哮喘小鼠,观察其肺、皮、肠组织中上皮源性细胞因子的变化。方法:将C57/BL6雌性小鼠分为空白对照组、哮喘模型组、地塞米松组、麻芥巴布膏组。使用卵白蛋白(ovalbumin,OVA)为致敏剂诱导建立哮喘小鼠模型,计算脾指数,使用苏木精-伊红染色观察肺组织的病理改变,酶联免疫吸附测定检测血清中免疫球蛋白E(immunoglobulin E,IgE),及肺、皮、肠组织中胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)的含量;实时荧光定量PCR法检测皮、肠组织中TSLP mRNA、白细胞介素-33(IL-33)mRNA的表达。结果:与空白对照组比较,哮喘模型组小鼠脾指数增高,并出现血管内充血水肿,炎性细胞浸润和支气管壁增厚的病理变化,血清中IgE的含量增多,TSLP含量在肺、皮组织中增多,肠组织中没有明显变化,TSLP mRNA的表达在皮、肠组织中显著上调,而IL-33 mRNA的表达在皮组织中显著上调(P<0.05),但在肠组织中无显著性差异(P>0.05)。与模型对照组比较,麻芥巴布膏可降低脾指数和血清中IgE的含量,并可改善肺组织病理损伤,减少肺、皮、肠组织中TSLP的含量,增加皮组织中TSLP mRNA表达,下调皮组织中IL-33 mRNA表达以及肠组织中TSLP mRNA、IL-33 mRNA的表达,差异均有统计学意义(P<0.05)。在肺组织与皮组织间,实验各组TSLP含量的相关性为0.689,肺组织与肠组织间的相关性为−0.163,皮组织和肠组织间的相关性为0.422,差异均无统计学意义(P>0.05)。结论:麻芥巴布膏可以降低OVA诱导的哮喘小鼠“肺-皮-肠”轴上皮源性细胞因子的含量,从而改善气道炎症,达到治疗哮喘的作用。