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Optimized prime editing efficiently generates heritable mutations in maize 被引量:8
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作者 Dexin Qiao Junya Wang +8 位作者 Min‐Hui Lu Cuiping Xin Yiping Chai Yuanyuan Jiang Wei Sun Zhenghong Cao Siyi Guo Xue‐Chen Wang Qi‐Jun Chen 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2023年第4期900-906,共7页
Low efficiency is the main obstacle to using prime editing in maize(Zea mays).Recently,prime-editing efficiency was greatly improved in mammalian cells and rice(Oryza sativa)plants by engineering primeediting guide RN... Low efficiency is the main obstacle to using prime editing in maize(Zea mays).Recently,prime-editing efficiency was greatly improved in mammalian cells and rice(Oryza sativa)plants by engineering primeediting guide RNAs(pegRNAs),optimizing the prime editor(PE)protein,and manipulating cellular determinants of prime editing.In this study,we tested PEs optimized via these three strategies in maize.We demonstrated that the ePE5max system,composed of PEmax,epegRNAs(pegRNA-evopreQ.1),nicking single guide RNAs(sgRNAs),and MLH1dn,efficiently generated heritable mutations that conferred resistance to herbicides that inhibit 5-enolpyruvylshikimate-3-phosphate synthase(EPSPS),acetolactate synthase(ALS),or acetyl CoA carboxylase(ACCase)activity.Collectively,we demonstrate that the ePE5max system has sufficient efficiency to generate heritable(homozygous or heterozygous)mutations in maize target genes and that the main obstacle to using PEs in maize has thus been removed. 展开更多
关键词 CRISPR/Cas epegRNAs herbicide resistance MAIZE mlh1dn prime editing
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