目的观察人滑膜细胞Wnt/β-catenin信号通路激活后对其下游基因MMP-2、MMP-7和MMP-9的影响。方法采用GSK-3β选择性抑制剂SB-216763作用于正常人滑膜细胞,提取胞核蛋白β-catenin,采用Western blotting检测胞核蛋白β-catenin的表达变化...目的观察人滑膜细胞Wnt/β-catenin信号通路激活后对其下游基因MMP-2、MMP-7和MMP-9的影响。方法采用GSK-3β选择性抑制剂SB-216763作用于正常人滑膜细胞,提取胞核蛋白β-catenin,采用Western blotting检测胞核蛋白β-catenin的表达变化;采用酶联免疫吸附法(ELISA)检测人滑膜细胞上清液中MMP-2、MMP-7和MMP-9的表达变化。结果 Western blotting结果显示,GSK-3β选择性抑制剂SB-216763作用于人滑膜细胞后,β-catenin在胞核蛋白中的表达水平明显升高,并随着GSK-3β选择性抑制剂作用时间的延长,β-catenin的表达逐渐增强,呈时间依赖性,而在其干预的第48小时,胞核蛋白β-catenin的表达变化最明显,是正常组滑膜细胞的5.8696倍,具有统计学意义(P<0.05);酶联免疫吸附法(ELISA)结果显示,与正常组滑膜细胞比较,SB-216763干预的滑膜细胞上清液中MMP-2、MMP-7和MMP-9的表达分别是正常滑膜细胞的4.6188、3.2443和2.9979倍,具有统计学意义(P<0.05)。结论①SB-216763成功激活人滑膜细胞Wnt/β-catenin信号通路;②MMP-2、7、9在人滑膜细胞Wnt/β-catenin信号通路激活后其表达显著升高,证明激活Wnt/β-catenin信号通路对MMP-2、MMP-7和MMP-9的表达有一定的调控作用。③Wnt/β-catenin信号通路在骨性关节炎患者中是激活的,而其对MMP-2、MMP-7和MMP-9的调控可能是导致关节软骨降解,促进骨关节炎形成的作用机制之一。该实验结果有助于从单一通路阐释Wnt/β-catenin信号通路对膝骨关节炎的作用机制。展开更多
Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: ...Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: A total of 50 patients with gastric cancer, and 50 healthy persons were selected as the experimental group, benign gastric disease group and healthy group, respectively. A total of 24 patients in experimental group met surgery indications, and agreed to perform gastrectomy. They were divided into preoperative group and postoperative group. The serums levels of KLK7 and E-cad were detected by enzyme-linked immunosorbent assay.Results: The serum levels of KLK7 and E-cad were significantly higher than that of benign gastric disease group and normal control group. Compared with the experimental group after operation, MMP-2 and MMP-9 were decreased, KLK7 was significantly decreased and E-cad increased significantly after operation. Serum levels of KLK7 and E-cad in experimental group stage I were increased and stage IV decreased. Microvascular invasion was significantly correlated with serum KLK7 and E-cad. There was no significant correlation between tumor size, differentiation degree, depth and expression of KLK7 and E-cad. Serum KLK7 were significantly negatively correlated with E-cad expression in gastric cancer patients. Conclusions:KLK7 and E-cad are involved in the development and metastasis of gastric cancer. The serum expression of KLK7 and E-cad are significantly negatively correlated in patients with stomach cancer. There may be an antagonistic effect in gastric cancer development and metastasis. Serum level of KLK7 and E-cad can be used as indexes for progress monitoring.展开更多
文摘目的观察人滑膜细胞Wnt/β-catenin信号通路激活后对其下游基因MMP-2、MMP-7和MMP-9的影响。方法采用GSK-3β选择性抑制剂SB-216763作用于正常人滑膜细胞,提取胞核蛋白β-catenin,采用Western blotting检测胞核蛋白β-catenin的表达变化;采用酶联免疫吸附法(ELISA)检测人滑膜细胞上清液中MMP-2、MMP-7和MMP-9的表达变化。结果 Western blotting结果显示,GSK-3β选择性抑制剂SB-216763作用于人滑膜细胞后,β-catenin在胞核蛋白中的表达水平明显升高,并随着GSK-3β选择性抑制剂作用时间的延长,β-catenin的表达逐渐增强,呈时间依赖性,而在其干预的第48小时,胞核蛋白β-catenin的表达变化最明显,是正常组滑膜细胞的5.8696倍,具有统计学意义(P<0.05);酶联免疫吸附法(ELISA)结果显示,与正常组滑膜细胞比较,SB-216763干预的滑膜细胞上清液中MMP-2、MMP-7和MMP-9的表达分别是正常滑膜细胞的4.6188、3.2443和2.9979倍,具有统计学意义(P<0.05)。结论①SB-216763成功激活人滑膜细胞Wnt/β-catenin信号通路;②MMP-2、7、9在人滑膜细胞Wnt/β-catenin信号通路激活后其表达显著升高,证明激活Wnt/β-catenin信号通路对MMP-2、MMP-7和MMP-9的表达有一定的调控作用。③Wnt/β-catenin信号通路在骨性关节炎患者中是激活的,而其对MMP-2、MMP-7和MMP-9的调控可能是导致关节软骨降解,促进骨关节炎形成的作用机制之一。该实验结果有助于从单一通路阐释Wnt/β-catenin信号通路对膝骨关节炎的作用机制。
文摘Objective:To explore the expression level of klk7 and E-cad in serum of patients with gastric cancer and to explore the relationship of klk7 and E-cad with gastric cancer clinical pathological characteristics.Method: A total of 50 patients with gastric cancer, and 50 healthy persons were selected as the experimental group, benign gastric disease group and healthy group, respectively. A total of 24 patients in experimental group met surgery indications, and agreed to perform gastrectomy. They were divided into preoperative group and postoperative group. The serums levels of KLK7 and E-cad were detected by enzyme-linked immunosorbent assay.Results: The serum levels of KLK7 and E-cad were significantly higher than that of benign gastric disease group and normal control group. Compared with the experimental group after operation, MMP-2 and MMP-9 were decreased, KLK7 was significantly decreased and E-cad increased significantly after operation. Serum levels of KLK7 and E-cad in experimental group stage I were increased and stage IV decreased. Microvascular invasion was significantly correlated with serum KLK7 and E-cad. There was no significant correlation between tumor size, differentiation degree, depth and expression of KLK7 and E-cad. Serum KLK7 were significantly negatively correlated with E-cad expression in gastric cancer patients. Conclusions:KLK7 and E-cad are involved in the development and metastasis of gastric cancer. The serum expression of KLK7 and E-cad are significantly negatively correlated in patients with stomach cancer. There may be an antagonistic effect in gastric cancer development and metastasis. Serum level of KLK7 and E-cad can be used as indexes for progress monitoring.
