目的检测MMP12 m RNA在人肺腺癌组织中的表达,探讨其与临床病理特征的关系。方法收集肺腺癌患者肿瘤组织样本39例,每例患者另取癌旁组织作为对照,应用real-time PCR技术,分别以GAPDH、RPLP0、PUM1、HPRT1和TBP为内参基因分析肺腺癌肿瘤...目的检测MMP12 m RNA在人肺腺癌组织中的表达,探讨其与临床病理特征的关系。方法收集肺腺癌患者肿瘤组织样本39例,每例患者另取癌旁组织作为对照,应用real-time PCR技术,分别以GAPDH、RPLP0、PUM1、HPRT1和TBP为内参基因分析肺腺癌肿瘤组织及癌旁组织MMP12 m RNA表达水平。扩大样本至103例,以PUM1为内参基因分析肺腺癌肿瘤组织及癌旁组织MMP12 m RNA表达水平及MMP12 m RNA表达水平与患者临床病理特征的关系。结果肺腺癌肿瘤组织相对于癌旁组织MMP12 m RNA表达水平上调者所占比例较表达水平无改变或表达水平下调者增高,且以PUM1为内参基因检测肺腺癌肿瘤组织MMP12 m RNA表达水平高于癌旁组织(P<0.05),且MMP12 m RNA在男性患者(男性vs女性,2.07±3.25 vs 0.53±2.90,P<0.05)、病理亚型为实性/微乳头状生长为主浸润性腺癌(实性/微乳头状生长为主vs附壁样/乳头状/腺泡状生长为主,3.30±2.95 vs0.75±3.20,P<0.05)中表达水平上调。结论 MMP12 m RNA在肺腺癌肿瘤组织中表达水平上调,可能参与肺腺癌发生、发展,且性别可能作为肺腺癌肿瘤组织MMP12 m RNA表达水平上调的危险因素。展开更多
Topographical properties,such as pattern and diameter,of biomaterials play important roles in influencing cell activities and manipulating the related immune response during wound healing.We prepared aligned electrosp...Topographical properties,such as pattern and diameter,of biomaterials play important roles in influencing cell activities and manipulating the related immune response during wound healing.We prepared aligned electrospinning membranes with different fiber diameters,including 319±100 nm(A300),588±132 nm(A600),and 1048±130 nm(A1000),by adjusting the distance from the tip to the collector,the injection rate,and the concentration of the solution.The A300 membranes significantly improved cell proliferation and spreading and facilitated wound healing(epithelization and vascularization)with the regeneration of immature hair follicles compared to the other membranes.Transcriptomics revealed the underlying molecular mechanism that A300 could promote immune-related processes towards a pro-healing direction,significantly promoting keratinocyte migration and skin wound healing.All the results indicated that wound healing requires the active participation of the immune process,and that A300 was a potential candidate for guided skin regeneration applications.展开更多
文摘目的检测MMP12 m RNA在人肺腺癌组织中的表达,探讨其与临床病理特征的关系。方法收集肺腺癌患者肿瘤组织样本39例,每例患者另取癌旁组织作为对照,应用real-time PCR技术,分别以GAPDH、RPLP0、PUM1、HPRT1和TBP为内参基因分析肺腺癌肿瘤组织及癌旁组织MMP12 m RNA表达水平。扩大样本至103例,以PUM1为内参基因分析肺腺癌肿瘤组织及癌旁组织MMP12 m RNA表达水平及MMP12 m RNA表达水平与患者临床病理特征的关系。结果肺腺癌肿瘤组织相对于癌旁组织MMP12 m RNA表达水平上调者所占比例较表达水平无改变或表达水平下调者增高,且以PUM1为内参基因检测肺腺癌肿瘤组织MMP12 m RNA表达水平高于癌旁组织(P<0.05),且MMP12 m RNA在男性患者(男性vs女性,2.07±3.25 vs 0.53±2.90,P<0.05)、病理亚型为实性/微乳头状生长为主浸润性腺癌(实性/微乳头状生长为主vs附壁样/乳头状/腺泡状生长为主,3.30±2.95 vs0.75±3.20,P<0.05)中表达水平上调。结论 MMP12 m RNA在肺腺癌肿瘤组织中表达水平上调,可能参与肺腺癌发生、发展,且性别可能作为肺腺癌肿瘤组织MMP12 m RNA表达水平上调的危险因素。
基金supported by Key R&D Projects of Sichuan Science and Technology Plan(2021YFS0030)Interdisciplinary Innovation Project,West China Hospital of Stomatology Sichuan University(RD-03-202006)Research and Develop Program,West China Hospital of Stomatology Sichuan University(No.LCYJ2019-19).
文摘Topographical properties,such as pattern and diameter,of biomaterials play important roles in influencing cell activities and manipulating the related immune response during wound healing.We prepared aligned electrospinning membranes with different fiber diameters,including 319±100 nm(A300),588±132 nm(A600),and 1048±130 nm(A1000),by adjusting the distance from the tip to the collector,the injection rate,and the concentration of the solution.The A300 membranes significantly improved cell proliferation and spreading and facilitated wound healing(epithelization and vascularization)with the regeneration of immature hair follicles compared to the other membranes.Transcriptomics revealed the underlying molecular mechanism that A300 could promote immune-related processes towards a pro-healing direction,significantly promoting keratinocyte migration and skin wound healing.All the results indicated that wound healing requires the active participation of the immune process,and that A300 was a potential candidate for guided skin regeneration applications.
