M M P-2、9同属于基质金属蛋白酶（matrix metalloproteinases ，M M Ps）家族中的明胶酶类，并广泛分布于生物体内，包括间质细胞、内皮细胞和上皮细胞等。MMP-2（明胶酶A）相对分子质量为72 Kd；MMP-9（明胶酶 B）相对分子质量为92 Kd...M M P-2、9同属于基质金属蛋白酶（matrix metalloproteinases ，M M Ps）家族中的明胶酶类，并广泛分布于生物体内，包括间质细胞、内皮细胞和上皮细胞等。MMP-2（明胶酶A）相对分子质量为72 Kd；MMP-9（明胶酶 B）相对分子质量为92 Kd。MMP-2、9的主要作用是降解和重塑细胞外基质（ECM ），可参与伤口愈合、骨吸收、器官形成、炎症、血管疾病、自身免疫性疾病及癌症发生等过程。近十几年来，人们对MMP-2、9的不断研究，为开发各种 M M Ps 抑制剂指出了新的方向。新型选择性MMPs抑制剂MMI-166，可特异性抑制MMP-2、9的活性，同时克服了前一、二代抑制剂的副作用，成为了人们研究的热点。展开更多
目的分析10-MDP-钙盐形成对牙本质粘接成绩的影响。方法采用酸蚀冲洗粘接模式,根据牙本质表面的处理方式和选择粘接剂的不同将牙齿随机分为以下4组(n=5)进行处理,制作牙本质/树脂粘接试件:(1)对照组,直接使用全酸蚀粘接剂Single bond 2(...目的分析10-MDP-钙盐形成对牙本质粘接成绩的影响。方法采用酸蚀冲洗粘接模式,根据牙本质表面的处理方式和选择粘接剂的不同将牙齿随机分为以下4组(n=5)进行处理,制作牙本质/树脂粘接试件:(1)对照组,直接使用全酸蚀粘接剂Single bond 2(SB2)处理后粘接;(2)10-MDP组,使用SB2处理进行粘接前,牙本质表面以含有磷酸酯单体10-MDP的自配底涂剂预处理;(3)CHX组,使用SB2处理进行粘接前,先以氯己定(CHX)预处理牙本质表面;(4)SBU组,使用包含10-MDP的通用型粘接剂Single bond universal(SBU)处理后进行粘接。通过微拉伸测试(μTBS)测试粘接强度,以X射线衍射(XRD)、原位酶谱测试表征自配10-MDP底涂剂和两种牙本质粘接剂处理的牙本质表面,分析10-MDP-钙盐形成对牙本质粘接成绩的影响。结果微拉伸结果显示,不同处理方式的粘接试件在24 h水储后没有表现出明显的统计学差异(P>0.1);经过6个月的水储后,与10-MDP组和SBU组相比,对照组的微拉伸强度显著降低(P<0.05),而CHX组的微拉伸强度没有明显变化(P>0.05)。XRD结果显示,在10-MDP组和SBU组均检测到10-MDP-钙盐形成的特征性峰,表明有10-MDP-钙盐的形成。原位酶谱结果显示,10-MDP组与SBU组之间混合层荧光强度没有明显区别,但均明显高于对照组,CHX组荧光强度低于10-MDP组与SBU组。结论10-MDP-钙盐的形成能够保护暴露的胶原纤维不接触到MMPs而免于水解,从而增强牙本质/树脂的粘接成绩。展开更多
Acne vulgaris is a skin disorder of the sebaceous follicles, involving hyperkeratinization and perifollicular inflammation. Matrix metalloproteinases (MMP) have a predominant role in inflammatory matrix remodeling and...Acne vulgaris is a skin disorder of the sebaceous follicles, involving hyperkeratinization and perifollicular inflammation. Matrix metalloproteinases (MMP) have a predominant role in inflammatory matrix remodeling and hyperproliferative skin disorders. We investigated the expression of MMP and tissue inhibitors of MMP(TIMP) in facial sebum specimens from acne patients, before and after treatment with isotretinoin. Gelatin zymography and Western-blot analysis revealed that sebum contains proMMP-9, which was decreased following per os or topical treatment with isotretinoin and in parallel to the clinical improvement of acne. Sebum also contains MMP-1, MMP-13, TIMP-1, and TIMP-2, as assessed by ELISA and western blot, but only MMP-13 was decreased following treatment with isotretinoin. The origin of MMP and TIMP in sebum is attributed to keratinocytes and sebocytes, since we found that HaCaT keratinocytes in culture secrete proMMP-2, proMMP-9,MMP- 1, MMP-13, TIMP-1, and TIMP-2. SZ95 sebocytes in culture secreted proMMP-2 and proMMP-9, which was also confirmed by microarray analysis. Isotretinoin inhibited the arachidonic acid-induced secretion and mRNA expression of proMMP-2 and -9 in both cell types and of MMP-13 in HaCaT keratinocytes. These data indicate that MMP and TIMP of epithelial origin may be involved in acne pathogenesis, and that isotretinoin- induced reduction in MMP-9 and -13 may contribute to the therapeutic effects of the agent in acne.展开更多
文摘M M P-2、9同属于基质金属蛋白酶（matrix metalloproteinases ，M M Ps）家族中的明胶酶类，并广泛分布于生物体内，包括间质细胞、内皮细胞和上皮细胞等。MMP-2（明胶酶A）相对分子质量为72 Kd；MMP-9（明胶酶 B）相对分子质量为92 Kd。MMP-2、9的主要作用是降解和重塑细胞外基质（ECM ），可参与伤口愈合、骨吸收、器官形成、炎症、血管疾病、自身免疫性疾病及癌症发生等过程。近十几年来，人们对MMP-2、9的不断研究，为开发各种 M M Ps 抑制剂指出了新的方向。新型选择性MMPs抑制剂MMI-166，可特异性抑制MMP-2、9的活性，同时克服了前一、二代抑制剂的副作用，成为了人们研究的热点。
文摘目的分析10-MDP-钙盐形成对牙本质粘接成绩的影响。方法采用酸蚀冲洗粘接模式,根据牙本质表面的处理方式和选择粘接剂的不同将牙齿随机分为以下4组(n=5)进行处理,制作牙本质/树脂粘接试件:(1)对照组,直接使用全酸蚀粘接剂Single bond 2(SB2)处理后粘接;(2)10-MDP组,使用SB2处理进行粘接前,牙本质表面以含有磷酸酯单体10-MDP的自配底涂剂预处理;(3)CHX组,使用SB2处理进行粘接前,先以氯己定(CHX)预处理牙本质表面;(4)SBU组,使用包含10-MDP的通用型粘接剂Single bond universal(SBU)处理后进行粘接。通过微拉伸测试(μTBS)测试粘接强度,以X射线衍射(XRD)、原位酶谱测试表征自配10-MDP底涂剂和两种牙本质粘接剂处理的牙本质表面,分析10-MDP-钙盐形成对牙本质粘接成绩的影响。结果微拉伸结果显示,不同处理方式的粘接试件在24 h水储后没有表现出明显的统计学差异(P>0.1);经过6个月的水储后,与10-MDP组和SBU组相比,对照组的微拉伸强度显著降低(P<0.05),而CHX组的微拉伸强度没有明显变化(P>0.05)。XRD结果显示,在10-MDP组和SBU组均检测到10-MDP-钙盐形成的特征性峰,表明有10-MDP-钙盐的形成。原位酶谱结果显示,10-MDP组与SBU组之间混合层荧光强度没有明显区别,但均明显高于对照组,CHX组荧光强度低于10-MDP组与SBU组。结论10-MDP-钙盐的形成能够保护暴露的胶原纤维不接触到MMPs而免于水解,从而增强牙本质/树脂的粘接成绩。
文摘Acne vulgaris is a skin disorder of the sebaceous follicles, involving hyperkeratinization and perifollicular inflammation. Matrix metalloproteinases (MMP) have a predominant role in inflammatory matrix remodeling and hyperproliferative skin disorders. We investigated the expression of MMP and tissue inhibitors of MMP(TIMP) in facial sebum specimens from acne patients, before and after treatment with isotretinoin. Gelatin zymography and Western-blot analysis revealed that sebum contains proMMP-9, which was decreased following per os or topical treatment with isotretinoin and in parallel to the clinical improvement of acne. Sebum also contains MMP-1, MMP-13, TIMP-1, and TIMP-2, as assessed by ELISA and western blot, but only MMP-13 was decreased following treatment with isotretinoin. The origin of MMP and TIMP in sebum is attributed to keratinocytes and sebocytes, since we found that HaCaT keratinocytes in culture secrete proMMP-2, proMMP-9,MMP- 1, MMP-13, TIMP-1, and TIMP-2. SZ95 sebocytes in culture secreted proMMP-2 and proMMP-9, which was also confirmed by microarray analysis. Isotretinoin inhibited the arachidonic acid-induced secretion and mRNA expression of proMMP-2 and -9 in both cell types and of MMP-13 in HaCaT keratinocytes. These data indicate that MMP and TIMP of epithelial origin may be involved in acne pathogenesis, and that isotretinoin- induced reduction in MMP-9 and -13 may contribute to the therapeutic effects of the agent in acne.
