稻瘟病菌(Magnaporthe oryzae)引起的水稻稻瘟病,每年造成全球水稻减产约10%~30%,对全球粮食生产构成严重威胁。MOB(Mps one binder)蛋白家族成员在物种进化过程中高度保守,并作为重要激酶调控因子与NDR(Nuclear DBF2-related)蛋白激酶...稻瘟病菌(Magnaporthe oryzae)引起的水稻稻瘟病,每年造成全球水稻减产约10%~30%,对全球粮食生产构成严重威胁。MOB(Mps one binder)蛋白家族成员在物种进化过程中高度保守,并作为重要激酶调控因子与NDR(Nuclear DBF2-related)蛋白激酶等结合,在细胞周期的有丝分裂退出和胞质分离等事件中发挥重要作用。本研究利用功能丧失和获得的方法建立了MoMOB1敲除突变体,对突变体进行了功能回补;并对背景菌株、敲除突变体和回补菌株,在形态特征和致病性等方面进行了生物性状分析,以解析稻瘟病菌中MoMOB1的生物学功能。与背景菌株Ku80相比,MoMOB1缺失后稻瘟病菌生长速率明显减慢,分生孢子萌发延迟,突变体产孢量显著降低,分生孢子出现畸形且隔膜数目异常,部分分生孢子没有隔膜或只有一个隔膜。致病性分析发现,ΔMoMOB1突变体致病性明显减弱。回补菌株的表型与背景菌株Ku80相似。综上所述,MoMOB1在稻瘟病菌营养生长、产孢量、分生孢子发育、附着胞形成和致病性方面发挥关键作用。展开更多
The microRNAs (miRNAs) play an important role in regulating myogenesis by targeting mRNA. However, the understanding of miRNAs in skeletal muscle development and diseases is unclear. In this study, we firstly performe...The microRNAs (miRNAs) play an important role in regulating myogenesis by targeting mRNA. However, the understanding of miRNAs in skeletal muscle development and diseases is unclear. In this study, we firstly performed the transcriptome profiling in differentiating C2C12 myoblast cells. Totally, we identified 187 miRNAs and 4260 mRNAs significantly differentially expressed that were involved in myoblast differentiation. We carried out validation of microarray data based on 5 mRNAs and 5 miRNAs differentially expressed and got a consistent result. Then we constructed and validated the significantly up- and down-regulated mRNA-miRNA interaction networks. Four interaction pairs (miR-145a-5p-Fscn1, miR-200c-5p-Tmigd1, miR-27a-5p-Sln and miR-743a-5p-Mob1b) with targeted relationships in differentiated myoblast cells were demonstrated. They are all closely related to myoblast development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated cell cycle signals important for exploring skeletal muscle development and disease. Functionally, we discovered that miR-743a targeting gene Mps One Binder Kinase Activator-Like 1B (Mob1b) gene in differentiated C2C12. The up-regulated miR-743a can promote the differentiation of C2C12 myoblast. While the down-regulated Mob1b plays a negative role in differentiation. In addition, the expression profile of miR-743a and Mob1b are consistent with skeletal muscle recovery after Cardiotoxin (CTX) injury. Our study revealed that miR-743a-5p regulates myoblast differentiation by targeting Mob1b involved in skeletal muscle development and regeneration. Our findings made a further exploration for mechanisms in myogenesis and might provide potential possible miRNA-based target therapies for skeletal muscle regeneration and disease in the near future.展开更多
文摘稻瘟病菌(Magnaporthe oryzae)引起的水稻稻瘟病,每年造成全球水稻减产约10%~30%,对全球粮食生产构成严重威胁。MOB(Mps one binder)蛋白家族成员在物种进化过程中高度保守,并作为重要激酶调控因子与NDR(Nuclear DBF2-related)蛋白激酶等结合,在细胞周期的有丝分裂退出和胞质分离等事件中发挥重要作用。本研究利用功能丧失和获得的方法建立了MoMOB1敲除突变体,对突变体进行了功能回补;并对背景菌株、敲除突变体和回补菌株,在形态特征和致病性等方面进行了生物性状分析,以解析稻瘟病菌中MoMOB1的生物学功能。与背景菌株Ku80相比,MoMOB1缺失后稻瘟病菌生长速率明显减慢,分生孢子萌发延迟,突变体产孢量显著降低,分生孢子出现畸形且隔膜数目异常,部分分生孢子没有隔膜或只有一个隔膜。致病性分析发现,ΔMoMOB1突变体致病性明显减弱。回补菌株的表型与背景菌株Ku80相似。综上所述,MoMOB1在稻瘟病菌营养生长、产孢量、分生孢子发育、附着胞形成和致病性方面发挥关键作用。
基金This work was supported by the Basic and Applied Basic Research Foundation of Guangdong province (2019B1515120059), the National Natural Science Foundation of China (No. 31830090), Shenzhen Key Technology Projects (JSGG20180507182028625), the National Key Project (No. 2016ZX08009-003-006), and Major Tasks of Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences (No. CAAS-ZDRW202006).
文摘The microRNAs (miRNAs) play an important role in regulating myogenesis by targeting mRNA. However, the understanding of miRNAs in skeletal muscle development and diseases is unclear. In this study, we firstly performed the transcriptome profiling in differentiating C2C12 myoblast cells. Totally, we identified 187 miRNAs and 4260 mRNAs significantly differentially expressed that were involved in myoblast differentiation. We carried out validation of microarray data based on 5 mRNAs and 5 miRNAs differentially expressed and got a consistent result. Then we constructed and validated the significantly up- and down-regulated mRNA-miRNA interaction networks. Four interaction pairs (miR-145a-5p-Fscn1, miR-200c-5p-Tmigd1, miR-27a-5p-Sln and miR-743a-5p-Mob1b) with targeted relationships in differentiated myoblast cells were demonstrated. They are all closely related to myoblast development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated cell cycle signals important for exploring skeletal muscle development and disease. Functionally, we discovered that miR-743a targeting gene Mps One Binder Kinase Activator-Like 1B (Mob1b) gene in differentiated C2C12. The up-regulated miR-743a can promote the differentiation of C2C12 myoblast. While the down-regulated Mob1b plays a negative role in differentiation. In addition, the expression profile of miR-743a and Mob1b are consistent with skeletal muscle recovery after Cardiotoxin (CTX) injury. Our study revealed that miR-743a-5p regulates myoblast differentiation by targeting Mob1b involved in skeletal muscle development and regeneration. Our findings made a further exploration for mechanisms in myogenesis and might provide potential possible miRNA-based target therapies for skeletal muscle regeneration and disease in the near future.
