A trial ofAvicennia officinalis L. with five different spacings was conducted in the newly accreted lands along the western coastline （Patuakhali district） of Bangladesh since 1993 to assess growth perform- ance and...A trial ofAvicennia officinalis L. with five different spacings was conducted in the newly accreted lands along the western coastline （Patuakhali district） of Bangladesh since 1993 to assess growth perform- ance and the effect of spacing on tree growth. Data on tree density, height, diameter, bole height, crown diameter were recorded and ana- lyzed when the stand was 19 years old. Mean height ranged from 12.89-13.52 m and diameter at breast height （dbh） from 26.57-32.16 cm in plots of different spacings. The mean annual height increment ranged from 0,67-0.71 m, mean annual diameter increment from 1.40-1.69 cm and wood volume from 6.02-10.04 m3.ha＇l.al in different treatments. Significantly greater diameter （32.16 cm） and wood volume （10.04 m3.hal.a-1） were obtained with wider （2.13 m × 2.13 m） spacing than with closer spacings. But tree growth was unaffected by other spacings. Growth data were also recorded from other .4. officinalis plantations raised by Forest Department （FD） on different islands in Patuakhali and Bhola districts of Bangladesh. At these sites, mean annual height incre- ment ranged from 0.33-0.62 m, mean annual diameter increment from 0.72-1,37 cm and wood volume from 1.55-5.73 m3·ha-1·a·-1. The growth performance of A. offindicated that the newly aecreted lands along the western shoreline may be suitable for raising Avicennia planta- tions for the enrichment of coastal vegetation.展开更多
Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos.Methods:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 h.The causative act...Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos.Methods:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 h.The causative action of a delay in yolk sac absorption by Bajitian was investigated by RT-PCR analysis of lipid metabolism-related microsomal triglyceride transfer protein(MTP),apolipoprotein CII(ApoC2)and lipogenesis-related liver x receptor(LXR)genes.The effect of Bajitian eliciting an inflammatory response was studied by exposing 72 hpf myeloperoxidase(MPO):GFP transgenic zebrafish embryos to Bajitian extract for 4 h.Assessment was done by TUNEL,caspase-3/7,and RT-PCR analysis of the apoptosis related pathway B-cell lymphoma 2 associated X protein(Bax),Nuclear factor kappa-lightchain-enhancer of activated B cells(NF-k B)genes,neutrophil development-related stem cell leukaemia(SCL)and transcription factor PU.1 genes,to reveal the causative action of Bajitian reducing neutrophils.Results:RT-PCR analysis found that Bajitian extract had no effect on the expression of MTP or ApoC2 genes,but upregulated LXR gene,which might explain the delay in yolk sac absorption.Analysis of the inflammatory response showed that compared with negative controls,Bajitian extract significantly(P<.05)reduced the number of neutrophils in MPO:GFP embryos.TUNEL,caspase-3/7,and RT-PCR analysis of Bax and NF-k B genes found that Bajitian extract did not trigger the cell apoptosis.Further RT-PCR analysis found that Bajitian extract did not affect SCL expression,but did lead to down-regulation of PU.1.The inhibition of neutrophil development/differentiation may explain the decline in the total number of neutrophils following Bajitian treatment,which could be attributed to the anti-inflammatory effects found clinically for this drug.Conclusions:This study demonstrated that Bajitian caused a delay in yolk sac absorption and a decrease neutrophil in zebrafish embryos,which may be related to the inhibition of neutrophil development.展开更多
An Asparagus officinalis L. cultivar NJ978 was used to study the growing rate, salt ion content and mineral ion uptake and distribution in plants under salt stress. The results showed that salt stress significantly in...An Asparagus officinalis L. cultivar NJ978 was used to study the growing rate, salt ion content and mineral ion uptake and distribution in plants under salt stress. The results showed that salt stress significantly inhibited seedling growth and there was a negative relationship between seedling growth and salt concentration. The seedlings growth showed no significant decrease under low salt stress （NaCl≤50 mmol/L）, while high concentrations of NaCl led to detrimental effects on the growth of A. officinalis L.. With the increase of NaCl concentration, the Na ＋ content increased gradually in A. officinalis L. roots, stems and leaves while the K^＋ and Ca^2＋ contents had slow decreases. Under low salt stress, the seedlings of A. officinalis L. could prevent Na ＋ from transporting to aerial part by withholding Na ＋ in root, which could keep the balance of ions in aerial part, but under high salt stress （NaCl 〉 100 mmol/L）, the aerial part of seedlings accumulated superabundance of Na ＋, which limited the uptake of K^＋ and Ca^2＋ , so seedlings were damaged heavily, which manifested as the gradual decrease of K^＋/Na ＋ and Ca^2＋ /Na ＋ in the roots and aerial part of the seedlings. The selective absorption of K^＋, Ca^2＋ and Na ＋ （AS K,Na and AS Ca,Na ） was significantly increased with increased salinity, while the selective transportation （TS K,Na and TS Ca,Na ） increased at first and then decreased with the increased salinity. Therefore, the strong ability of salt exclusion and the regionalized distribution of ions in roots, stems and leaves could be one of the salt tolerance mechanisms of A. officinalis L.展开更多
Objective:Based on the quality analysis of Nauclea officinalis in different growth years and different months in the same year,the most suitable harvest year and the specific month in the same year were determined acc...Objective:Based on the quality analysis of Nauclea officinalis in different growth years and different months in the same year,the most suitable harvest year and the specific month in the same year were determined according to the evaluation of the active components in the stem of medicinal parts of Nauclea officinalis.Methods:LC-MS/MS method was used to investigate the quality of Nauclea officinalis from different growth years and different months in the same year,mainly including the content determination of the active ingredients in the medicine.Results:With the prolongation of production life,the content of active ingredients in stem of Nauclea officinalis increased continuously.After reaching a certain number of years,the content of active ingredients increased little;In the middle of the same year,from mid-October to mid-June of the next year,the content of iso-vincin lactam was relatively high,especially between the end of March and the end of May.The content was high and stable,and the highest point was the stem sample collected in May.Conclusion:The content of each component in the stem of Nauclea officinalis with a growth life of more than 5 years has reached a relatively high and stable level.Nauclea officinalis with a growth life of more than 5 years should be collected as raw materials for medicinal materials.The month with high and stable content of isocyanoside lactam,namely May of each year,was taken as the best harvest time of gallbladder in one year.The best harvest time was from mid-October to mid-June of the next year,or from March to May of each year.展开更多
In this study, the allelopathic effects of 100 g/L water extract of Melilotus offtcinalis Desr. on seed germination and seedling growth of Melilotus offwinalis Desr., Trifolium pretense L. , Medicago sativa L. cv. Vic...In this study, the allelopathic effects of 100 g/L water extract of Melilotus offtcinalis Desr. on seed germination and seedling growth of Melilotus offwinalis Desr., Trifolium pretense L. , Medicago sativa L. cv. Victoria, Echinochloa crusgalli （ L. ） Beauv., Lxer/s chinensis （ Thunb. ） Nakai and Plantago asiatica L. were investigated by biological assay. Dried powder of Melilotus officinalis Desr. was applied to the field in spring and winter to verify the inhibitory effects of Me- lilotus officinalis Desr. on weed biomass in the experimental field and compare the effects between Melilotus offwinalis Desr. and a variety of commonly used chemi- cal herbicides on weed suppression. The results showed that water extract of Melilotm officinalis Desr. posed significant effects on seed germination and seedling growth of Trifolium pretense L. , Echinochloa crusgalli （ L. ） Beauv. , Lxeris chinensis （ Thunb. ） Nakm, Plantago asiatica L. , Melilotus o^tcinalis Desr. and Medi- cago sativa L. cv. Victoria, exhibiting strong allelopathic effects, but the inhibition rate varied greatly among different plant species. Therefore, the allelopathic effects of Melilotus o^winalis Desr. should be utilized appropriately in practical applieatians. Dried powder of Melilotus officinalis Desr. significantly reduced weed quantity in the field for a short duration, which should be administered multiple times in a sufficient amount for weed control in the production.展开更多
文摘A trial ofAvicennia officinalis L. with five different spacings was conducted in the newly accreted lands along the western coastline （Patuakhali district） of Bangladesh since 1993 to assess growth perform- ance and the effect of spacing on tree growth. Data on tree density, height, diameter, bole height, crown diameter were recorded and ana- lyzed when the stand was 19 years old. Mean height ranged from 12.89-13.52 m and diameter at breast height （dbh） from 26.57-32.16 cm in plots of different spacings. The mean annual height increment ranged from 0,67-0.71 m, mean annual diameter increment from 1.40-1.69 cm and wood volume from 6.02-10.04 m3.ha＇l.al in different treatments. Significantly greater diameter （32.16 cm） and wood volume （10.04 m3.hal.a-1） were obtained with wider （2.13 m × 2.13 m） spacing than with closer spacings. But tree growth was unaffected by other spacings. Growth data were also recorded from other .4. officinalis plantations raised by Forest Department （FD） on different islands in Patuakhali and Bhola districts of Bangladesh. At these sites, mean annual height incre- ment ranged from 0.33-0.62 m, mean annual diameter increment from 0.72-1,37 cm and wood volume from 1.55-5.73 m3·ha-1·a·-1. The growth performance of A. offindicated that the newly aecreted lands along the western shoreline may be suitable for raising Avicennia planta- tions for the enrichment of coastal vegetation.
基金Infinitus(China)Company Ltd.internal research funding。
文摘Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos.Methods:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 h.The causative action of a delay in yolk sac absorption by Bajitian was investigated by RT-PCR analysis of lipid metabolism-related microsomal triglyceride transfer protein(MTP),apolipoprotein CII(ApoC2)and lipogenesis-related liver x receptor(LXR)genes.The effect of Bajitian eliciting an inflammatory response was studied by exposing 72 hpf myeloperoxidase(MPO):GFP transgenic zebrafish embryos to Bajitian extract for 4 h.Assessment was done by TUNEL,caspase-3/7,and RT-PCR analysis of the apoptosis related pathway B-cell lymphoma 2 associated X protein(Bax),Nuclear factor kappa-lightchain-enhancer of activated B cells(NF-k B)genes,neutrophil development-related stem cell leukaemia(SCL)and transcription factor PU.1 genes,to reveal the causative action of Bajitian reducing neutrophils.Results:RT-PCR analysis found that Bajitian extract had no effect on the expression of MTP or ApoC2 genes,but upregulated LXR gene,which might explain the delay in yolk sac absorption.Analysis of the inflammatory response showed that compared with negative controls,Bajitian extract significantly(P<.05)reduced the number of neutrophils in MPO:GFP embryos.TUNEL,caspase-3/7,and RT-PCR analysis of Bax and NF-k B genes found that Bajitian extract did not trigger the cell apoptosis.Further RT-PCR analysis found that Bajitian extract did not affect SCL expression,but did lead to down-regulation of PU.1.The inhibition of neutrophil development/differentiation may explain the decline in the total number of neutrophils following Bajitian treatment,which could be attributed to the anti-inflammatory effects found clinically for this drug.Conclusions:This study demonstrated that Bajitian caused a delay in yolk sac absorption and a decrease neutrophil in zebrafish embryos,which may be related to the inhibition of neutrophil development.
基金Supported by the Science and Technology Support Program of Hebei Province(17226913D)the Project for Innovation of Hebei Province(F17R07)
文摘An Asparagus officinalis L. cultivar NJ978 was used to study the growing rate, salt ion content and mineral ion uptake and distribution in plants under salt stress. The results showed that salt stress significantly inhibited seedling growth and there was a negative relationship between seedling growth and salt concentration. The seedlings growth showed no significant decrease under low salt stress （NaCl≤50 mmol/L）, while high concentrations of NaCl led to detrimental effects on the growth of A. officinalis L.. With the increase of NaCl concentration, the Na ＋ content increased gradually in A. officinalis L. roots, stems and leaves while the K^＋ and Ca^2＋ contents had slow decreases. Under low salt stress, the seedlings of A. officinalis L. could prevent Na ＋ from transporting to aerial part by withholding Na ＋ in root, which could keep the balance of ions in aerial part, but under high salt stress （NaCl 〉 100 mmol/L）, the aerial part of seedlings accumulated superabundance of Na ＋, which limited the uptake of K^＋ and Ca^2＋ , so seedlings were damaged heavily, which manifested as the gradual decrease of K^＋/Na ＋ and Ca^2＋ /Na ＋ in the roots and aerial part of the seedlings. The selective absorption of K^＋, Ca^2＋ and Na ＋ （AS K,Na and AS Ca,Na ） was significantly increased with increased salinity, while the selective transportation （TS K,Na and TS Ca,Na ） increased at first and then decreased with the increased salinity. Therefore, the strong ability of salt exclusion and the regionalized distribution of ions in roots, stems and leaves could be one of the salt tolerance mechanisms of A. officinalis L.
基金Major science and technology planning project of Hainan province(No.ZDKJ201805)。
文摘Objective:Based on the quality analysis of Nauclea officinalis in different growth years and different months in the same year,the most suitable harvest year and the specific month in the same year were determined according to the evaluation of the active components in the stem of medicinal parts of Nauclea officinalis.Methods:LC-MS/MS method was used to investigate the quality of Nauclea officinalis from different growth years and different months in the same year,mainly including the content determination of the active ingredients in the medicine.Results:With the prolongation of production life,the content of active ingredients in stem of Nauclea officinalis increased continuously.After reaching a certain number of years,the content of active ingredients increased little;In the middle of the same year,from mid-October to mid-June of the next year,the content of iso-vincin lactam was relatively high,especially between the end of March and the end of May.The content was high and stable,and the highest point was the stem sample collected in May.Conclusion:The content of each component in the stem of Nauclea officinalis with a growth life of more than 5 years has reached a relatively high and stable level.Nauclea officinalis with a growth life of more than 5 years should be collected as raw materials for medicinal materials.The month with high and stable content of isocyanoside lactam,namely May of each year,was taken as the best harvest time of gallbladder in one year.The best harvest time was from mid-October to mid-June of the next year,or from March to May of each year.
基金Supported by Natural Science Foundation of China(31101764)Natural Science Fund for Colleges and Universities in Jiangsu Province(11KJB230004)
文摘In this study, the allelopathic effects of 100 g/L water extract of Melilotus offtcinalis Desr. on seed germination and seedling growth of Melilotus offwinalis Desr., Trifolium pretense L. , Medicago sativa L. cv. Victoria, Echinochloa crusgalli （ L. ） Beauv., Lxer/s chinensis （ Thunb. ） Nakai and Plantago asiatica L. were investigated by biological assay. Dried powder of Melilotus officinalis Desr. was applied to the field in spring and winter to verify the inhibitory effects of Me- lilotus officinalis Desr. on weed biomass in the experimental field and compare the effects between Melilotus offwinalis Desr. and a variety of commonly used chemi- cal herbicides on weed suppression. The results showed that water extract of Melilotm officinalis Desr. posed significant effects on seed germination and seedling growth of Trifolium pretense L. , Echinochloa crusgalli （ L. ） Beauv. , Lxeris chinensis （ Thunb. ） Nakm, Plantago asiatica L. , Melilotus o^tcinalis Desr. and Medi- cago sativa L. cv. Victoria, exhibiting strong allelopathic effects, but the inhibition rate varied greatly among different plant species. Therefore, the allelopathic effects of Melilotus o^winalis Desr. should be utilized appropriately in practical applieatians. Dried powder of Melilotus officinalis Desr. significantly reduced weed quantity in the field for a short duration, which should be administered multiple times in a sufficient amount for weed control in the production.
