Exploring the mechanism of action of herb pair Pinellia Ternata-Magnolia Officinalis in the treatment of liver cancer based on network pharmacology and molecular docking

Background:Explore the anti-tumor mechanism of herb pair Pinellia ternate-Magnolia officinalis(BX-HP)in liver cancer through network pharmacology using molecular docking methods.Method:The active ingredients and corresponding targets of the herb pair Pinellia ternate-Magnolia officinalis were obtained from the HERB database.The relevant targets for liver cancer were obtained from GeneCards,DisGeNET,TTD,and Drugbank databases.Obtain common targets between herb pair Pinellia ternate-Magnolia officinalis and liver cancer through the Bioinformatics platform,establish a PPI network diagram using STRING software,and perform GO functional enrichment and KEGG pathway enrichment analysis on the DAVID platform.AutoDockTools 1.5.7 software and molecular dynamics simulation analysis are used to evaluate the binding of components to target proteins.HERB database,SwissTargetPrediction database,SwissADME database,UniProt database,GeneCards database,TTD database,DRUGBANK database,DisGeNET database,String,DAVID.Bioinformatics platform,PDB database,PubChem and TCMSP database.Result:A total of 22 active ingredients with a Probability>0.1 targets in Magnolia officinalis were screened,26 active ingredients with a Probability>0.1 targets in Pinellia ternata,ten vital active ingredients,corresponding to 979 and 803 targets with a Probability>0.1 targets,2536 liver cancer-related targets,and 279 targets in the herb pair Pinellia ternata-Magnolia officinalis.The GO functional enrichment analysis resulted in 1297 entries,namely 971 biological process entries,118 cell localization entries,and 208 molecular function entries.Three signaling pathways were annotated through the KEGG pathway.Based on molecular docking,ten vital active ingredients and five target proteins were validated to exhibit an excellent binding affinity.The above data indicates that combining the herb pair Pinellia ternata-Magnolia officinalis may treat liver cancer through specific targets and signaling pathways.Conclusion:Herb pair Pinellia ternata-Magnolia officinalis has a synergistic effect on treating liver cancer through multicomponent,multitarget,and multi-pathway approaches.This study provides a sufficient theoretical basis for subsequent research.

Network pharmacology and preliminary cell screening studies on the anti-liver cancer activity of Nauclea Officinalis

Objective:To explore the mechanism of Nauclea Officinalis of anti-liver cancer effect based on network pharmacology,and to preliminarily verify anti-liver cancer activity of Nauclea Officinalis through cell screening.Methods:Network pharmacology was used to screen for common targets of Nauclea Officinalis and liver cancer,protein-protein interaction(PPI)network was constructed,and enrichment analysis and mechanism prediction were conductd.Molecular docking of main active ingredients of Nauclea Officinalis with core targets was made.Preliminary verification was performed by in vitro cell experiments such as CCK8,cell apoptosis,and PCR.Results:After the screening,14 active ingredients of Nauclea Officinalis were obtained,with 587 related targets.After mapping with liver cancer targets,there were 288 common targets,mainly including TP53,SRC,STAT3,and other core targets.Among them,compounds such as strictosamide,pumiloside and vincosamide may be potential active ingredients of Nauclea Officinalis of anti-liver cancer effect.They may participate in protein phosphorylation and negative regulation of the apoptosis process by mediating cancer pathways,PI3K/Akt and EGFR tyrosine kinase inhibitors resistance signaling pathways to play an anti-liver cancer role;molecular docking results showd that active ingredients of Nauclea Officinalis had a stable binding with liver cancer core targets;in vitro cell experiments showd that main ingredient strictosamide of Nauclea Officinalis had cytotoxicity against liver cancer cells,inhibited liver cancer cell proliferation(P<0.001),down-regulated gene expression of liver cancer HepG2 cells SRC,STAT3,MAPK3(P<0.05),and induced liver cancer cell apoptosis(P<0.001).Conclusion:This study preliminarily explores the potential mechanism of active ingredients of Nauclea Officinalis against liver cancer and its preliminary pharmacological effects,providing a theoretical basis for the study of Nauclea Officinalis of anti-liver cancer mechanism.

Mechanistic action of the acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos

Objectives:To investigate acute toxicity of Bajitian(Morinda officinalis)in zebrafish embryos.Methods:Zebrafish embryos at 48-h post fertilization(hpf)were exposed to Bajitian ethanol extract for72 h.The causative action of a delay in yolk sac absorption by Bajitian was investigated by RT-PCR analysis of lipid metabolism-related microsomal triglyceride transfer protein(MTP),apolipoprotein CII(ApoC2)and lipogenesis-related liver x receptor(LXR)genes.The effect of Bajitian eliciting an inflammatory response was studied by exposing 72 hpf myeloperoxidase(MPO):GFP transgenic zebrafish embryos to Bajitian extract for 4 h.Assessment was done by TUNEL,caspase-3/7,and RT-PCR analysis of the apoptosis related pathway B-cell lymphoma 2 associated X protein(Bax),Nuclear factor kappa-lightchain-enhancer of activated B cells(NF-k B)genes,neutrophil development-related stem cell leukaemia(SCL)and transcription factor PU.1 genes,to reveal the causative action of Bajitian reducing neutrophils.Results:RT-PCR analysis found that Bajitian extract had no effect on the expression of MTP or ApoC2 genes,but upregulated LXR gene,which might explain the delay in yolk sac absorption.Analysis of the inflammatory response showed that compared with negative controls,Bajitian extract significantly(P<.05)reduced the number of neutrophils in MPO:GFP embryos.TUNEL,caspase-3/7,and RT-PCR analysis of Bax and NF-k B genes found that Bajitian extract did not trigger the cell apoptosis.Further RT-PCR analysis found that Bajitian extract did not affect SCL expression,but did lead to down-regulation of PU.1.The inhibition of neutrophil development/differentiation may explain the decline in the total number of neutrophils following Bajitian treatment,which could be attributed to the anti-inflammatory effects found clinically for this drug.Conclusions:This study demonstrated that Bajitian caused a delay in yolk sac absorption and a decrease neutrophil in zebrafish embryos,which may be related to the inhibition of neutrophil development.

Research Situation of Polysaccharides of Dendrobii Officinalis Caulis

This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years, different periods, different habitats and different external factors had influences on the change of polysaccharide content in Den- drobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity, resisting fatigue and oxidation, promoting digestion and salivation secretion, reducing blood sugar and blood pressure, resisting tumor and liver injury, and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material.

Anti-tumor activity of Sanguisorba officinalis L.in non-small cell lung cancer and induced apoptosis via PI3K/Akt/mTOR signaling pathway

OBJECTIVE To investigate the pharmacological effect and mechanism of Sanguisorba officinalis L.(SOL)in non-small cell lung cancer(NSCLC)in vitro and in vivo based on network pharmacology.METHODS Network pharmacology was used to analyze the improving effect of SOL on NSCLC and possible targets.Cell counting kit 8(CCK-8)and 5-ethynyl-2′-deoxyuridine(EdU)staining,Western blotting,flow cytometry of AnnexinⅤ/PI,Hoechst 33342/PI staining detection and immunofluorescence were utilized in vitro.H&E staining,immunohistochemistry staining and Western blotting were performed in vivo.RESULTS Based on network prediction,we analyzed the 208 common targets of SOL and NSCLC.36 core targets in 208 common targets were obtained through cytoscape analysis.And the top 10 core targets included Akt,mTOR,EGFR,etc..KEGG analysis showed that PI3K-Akt signaling pathway was the most likely pathway.Furthermore,the mechanism study found that SOL could activate the PI3K/Akt/mTOR signaling pathway in vitro and in vivo.The anti-proliferative effect of SOL in A549 and H1299 cells was measured and validated by CCK-8 and EdU assay.Immunohistochemical results of Ki67 showed that SOL effectively inhibited tumor growth in vivo.SOL also significantly inhibited the migration and invasion of A549 and H1299 cells.SOL significantly increased the percentage of cells with PI signal in A549 and H1299,and the process of cell death of A549 cells indicated that SOL induced apoptosis.The PARP-1 and caspase-3 in A549 and H1299 were found to be activated in a dose manner.The results in vivo were consistent with those in vitro.CONCLUSION SOL-induced,caspase-3-mediated apoptosis via the induction of the PI3K/Akt/mTOR signaling pathway in NSCLC,which further clarified the mechanism of SOL in the inhibition of NSCLC,and thereby provided a possibility for SOL to serve as a novel therapeutic agent for NSCLC in the future.

柱前衍生-高效液相色谱法测定巴戟天中氨基酸含量及营养价值评价

建立柱前衍生-高效液相色谱法测定巴戟天中氨基酸含量,采用氨基酸比值系数法评价其营养价值。采用盐酸水解法制得巴戟天氨基酸供试品溶液,以异硫氰酸苯酯和三乙胺为柱前衍生化试剂,采用高效液相色谱法,使用Ultimate Amino Acid色谱柱(4.6 mm×250 mm,5μm)以0.1 mol/L无水乙酸钠-乙腈为流动相,梯度洗脱,柱温40℃,检测波长为254 nm,测定氨基酸含量;通过氨基酸比值、氨基酸比值系数、氨基酸比值系数分等评价其营养价值,并采用系统聚类和主成分分析对其进行分类分析。结果表明,15种氨基酸线性关系良好,相关系数r≥0.9996。16份巴戟天氨基酸总含量为17.87~102.15 mg/g,均检测到15种氨基酸,其中包含6种必需氨基酸,占总氨基酸含量的14.15%~32.06%;8种药用氨基酸,占总氨基酸含量的45.20%~56.31%。限制氨基酸为苏氨酸和亮氨酸,氨基酸比值系数分为33.87~61.31。不同巴戟天样品氨基酸组成相关性显著;主成分分析提取2个主成分,累计方差贡献率为97.085%,可将16批巴戟天分为三类。该实验测定方法简单有效,可同时分离测定15种氨基酸,精密度与重复性良好,有较高的药用价值,可为巴戟天的品质分析和资源开发利用提供参考。

近五年地榆的药理作用机制研究进展

传统中药地榆具有凉血止血、解毒敛疮的功效。现代研究对地榆的止血凝血作用、抗氧化作用、抗过敏作用、抗炎作用、抗菌作用、抗肿瘤作用、肾保护作用、升高白细胞作用等药理机制进行了广泛研究,机制明确。其中止血凝血作用机制是通过作用凝血酶及促进巨核细胞分化和血小板生成;抗氧化作用机制是上调抗氧化酶表达和清除氧自由基;抗过敏作用机制是抑制β-己糖氨酶和白介素-4的产生、释放或促进血红素加氧酶-1表达及激活;抗炎作用机制与巨噬细胞极化、聚集和迁移、脂质代谢、改善肠道菌群失调、信号传导与转录激活因子1及细胞外调节蛋白激酶激活、自噬、核因子κB/含pyrin结构域NOD样受体家族3、Toll样受体4/核因子κB、磷酯酰肌醇-3-激酶—丝氨酸/苏氨酸激酶/核因子κB/信号转导与转录激活因子3信号通路等相关;抗菌作用机制破坏细菌细胞壁、细胞膜结构和内部微观结构、干扰细菌总蛋白的合成、降低细胞膜流动性及改变代谢通路等相关基因表达,或抑制青霉素结合蛋白2a表达和mecA基因表达;抗肿瘤作用机制与血管内皮生长因子/磷酯酰肌醇-3-激酶—丝氨酸/苏氨酸激酶/雷帕霉素靶蛋白、表皮生长因子受体/丝裂原活化蛋白激酶表皮生长因子受体/磷酯酰肌醇-3-激酶—丝氨酸/苏氨酸激酶/核因子κB、缺氧诱导因子1、丝裂原化蛋白激酶、丝裂原活化蛋白激酶、肾素—血管紧张素/丝裂原活化的细胞外信号调节激酶/细胞外调节蛋白激酶、Wnt、自噬等信号通路及脂质磷酸酶活性相关;肾保护作用机制是抗氧化、抗炎、抗脂质过氧化、抑制肾小管上皮细胞的增殖;升高白细胞作用机制与自噬、造血系统相关通路和相关基因、造血生长因子等表达、糖代谢、氨基酸和核苷酸代谢的途径相关。在临床上用于消化道出血等出血症、过敏性紫癜及急性湿疹、烧烫伤、痤疮及压疮、急慢性肾小球肾炎等,且地榆制剂地榆升白片常用于白细胞减少症,临床效果明确,表明应用现代科学技术促进了传统中药地榆临床应用。本文对地榆的药理作用机制研究进行综述,为地榆的研究及临床应用提供参考。

不同产地巴戟天HPLC指纹图谱及化学模式识别研究

目的:利用HPLC指纹图谱及化学模式识别对巴戟天药材进行评价。方法:采用Shodex Asahipak NH2P-504E色谱柱(4.6 mm×250 mm,5μm),以乙腈-水为流动相梯度洗脱,使用蒸发光散射检测器(ELSD)。利用相似度评价、聚类分析和主成分分析等化学模式识别方法,对不同产地的巴戟天药材进行分析。结果:所建立的HPLC指纹图谱共识别了18个共有峰,经与对照品图谱比较,鉴定其中的4个共有峰分别为果糖、蔗糖、蔗果三糖和耐斯糖。6个产地巴戟天的相似度在0.861~0.995;经聚类分析和主成分分析,6个产地巴戟天共聚为3类。结论:本研究采用的巴戟天HPLC指纹图谱及化学模式识别技术可反映出不同产地的巴戟天样品的差异,可为巴戟天的质量控制提供参考。

基于BDNF/CREB信号通路探讨巴戟天对慢性应激抑郁大鼠海马神经元损伤的影响

目的研究巴戟天对慢性应激抑郁大鼠海马神经元损伤及脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)/细胞内环磷腺苷效应元件结合蛋白(cyclic adenosine phosphate response element binding protein,CREB)信号通路的影响。方法从40只SD大鼠随机选取其中10只为正常组,对其余大鼠构建慢性不可预知温和应激模型后,再将其分为3组,即模型组,盐酸氟西汀组(3.17 mg·kg^(-1)),巴戟天组(3.17 g·kg^(-1))。持续灌胃后给药8周,1次/d,并先后在造模前、造模后和给药后开展了行为学实验,以判断大鼠的抑郁情况。通过苏木素-伊红染色(hematoxylin-eosin staining,HE)研究大鼠海马形态学改变,用免疫组织化学法(Immunohistochemistry,IHC)测定大鼠海马BDNF蛋白表达,用HE染色研究大鼠海马组织病理损伤并评分,用实时荧光定量聚合酶链式反应(Real-time PCR,RT-PCR)测定大鼠海马BDNF、TrkB、CREB mRNA相对表达,用蛋白免疫印迹法(western blot,WB)测定大鼠海马BDNF、TrkB、CREB蛋白的相对表达。结果与正常组比较,模型组大鼠活动总路程显著减少(P<0.05),自主游泳时间显著减少(P<0.05),悬尾挣扎时间显著减少(P<0.05)。HE染色结果中表明海马神经元组织受到破坏,病理损伤评分显著下降(P<0.05),免疫组织化学染色中海马BDNF表现显著下降(P<0.05),BDNF、TrkB、CREB mRNA的基因相对表达显著下降(P<0.05);与模型组对比,盐酸氟西汀组和巴戟天组大鼠活动的总里程明显提高(P<0.05),自主游泳时间显著增加(P<0.05),悬尾挣扎时间显著增加(P<0.05),HE染色结果表明海马神经元组织明显复原,病理损伤评分增加(P<0.05),免疫组织化学染色中BDNF表现显著增加(P<0.05),BDNF、TrkB、CREB mRNA的基因相对表达明显增加(P<0.05)。结论经慢性应激刺激后,巴戟天可能通过激活BDNF/TrkB/CREB信号通路对抑郁大鼠海马神经元损伤发挥神经保护作用,改善其抑郁样行为。

基于网络药理学探讨川牛膝治疗高血压的作用机制

目的:利用网络药理学分析川牛膝治疗高血压的作用机制。方法:应用中药系统药理学数据库及分析平台(TCMSP)筛选出川牛膝的活性成分及其作用靶点,采用OMIM数据库,Drugbank数据库,TTD数据库,CTD数据库和DISGENT数据库检索与高血压相关的靶点基因。将川牛膝及高血压的共同靶点导入STRING数据库进行蛋白质-蛋白质相互作用分析(PPI);利用METASCAPE数据库进行核心靶点GO功能富集和KEGG通路富集分析。结果:川牛膝主要化学成分为槲皮素、杯苋甾酮、红苋甾酮、β-谷甾醇、齐墩果酸等;共筛选出关键共同靶点170个,包括AKT1、IL6、VEGFA、TP53、CASP3、TNF、JUN、EGF、MAPK1、EGFR、PTGS2、STAT3等;GO富集分析得到生物学进程主要涉及炎症反应、对伤害的反应、对脂多糖的反应、活性氧代谢过程、凋亡信号通路等;KEGG富集信号通路主要包含糖尿病并发症AGE-RAGE信号路径、肿瘤的途径、乙型肝炎、TNF信号通路、肿瘤中的蛋白质多糖等。结论:川牛膝治疗高血压存在多通路、多靶点的可能,揭示了川牛膝治疗高血压的潜在分子机制。

巴戟天多糖对文昌鸡生长性能、血清免疫和抗氧化指标及免疫器官发育的影响

【目的】本试验通过研究饲粮中添加巴戟天多糖(Morinda officinalis polysaccharide,MOP)对文昌鸡生长性能、血清免疫和抗氧化指标及免疫器官发育的影响,旨在为MOP在肉鸡生产中得到深入应用提供参考依据。【方法】选取6日龄体重相近、健康的雌性文昌鸡600只,随机分为5组,每组8个重复,每个重复15只鸡,对照组鸡饲喂基础饲粮,试验组鸡分别在基础饲粮中添加250、500、1000和2000 mg/kg的MOP,试验期40 d。在试验第1、40天分别称重,试验期间以重复为单位记录采食量和剩料量,计算平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G);在试验第40天每重复选取1只试验鸡采血,屠宰后采集胸腺、脾脏、法氏囊并称重,测定血清生化指标、抗氧化指标和免疫指标,并测定免疫器官指数。【结果】与对照组相比,①饲粮中添加不同水平的MOP对文昌鸡的终末体重、ADG和ADFI无显著影响(P>0.05),但250 mg/kg MOP组的F/G显著提高(P<0.05),其余试验组F/G无显著变化(P>0.05)。②250 mg/kg MOP组文昌鸡血清免疫球蛋白G(IgG)含量、500 mg/kg MOP组血清IgM含量均显著提高(P<0.05);500和1000 mg/kg MOP组血清丙二醛(MDA)含量显著降低(P<0.05);2000 mg/kg MOP组血清谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(P<0.05)。③500、1000和2000 mg/kg MOP组文昌鸡法氏囊指数均显著提高(P<0.05)。【结论】饲粮中添加250及500 mg/kg MOP可提高文昌鸡血清免疫球蛋白含量,添加500、1000和2000 mg/kg MOP可改善文昌鸡血清抗氧化指标,促进免疫器官法氏囊的生长发育。本试验中,MOP添加量以500 mg/kg为宜。

巴戟天多糖对文昌鸡肠道形态、肠道免疫屏障功能和盲肠微生物的影响

试验旨在研究饲粮中添加巴戟天多糖(Morinda officinalis polysaccharide,MOP)对文昌鸡肠道形态、肠道免疫屏障功能和盲肠微生物的影响。选取6日龄健康雌性文昌鸡600只,随机分为5组,每组8个重复,每个重复15只鸡。对照组饲喂基础饲粮,各试验组分别在基础饲粮中添加250、500、1000、2000 mg/kg的MOP。试验期40 d。结果表明:与对照组相比,各试验组十二指肠和空肠绒毛高度/隐窝深度均显著提高(P<0.05),500、1000、2000 mg/kg MOP组回肠绒毛高度/隐窝深度显著提高(P<0.05);250、500、1000 mg/kg MOP组十二指肠紧密连接蛋白Occludin mRNA相对表达量显著提高(P<0.05),1000、2000 mg/kg MOP组十二指肠肿瘤坏死因子-α(TNF-α)mRNA相对表达量显著降低(P<0.05);250、500 mg/kg MOP组盲肠观测物种数和Ace指数显著提高(P<0.05);各试验组Shannon指数均显著上升(P<0.05);2000 mg/kg MOP组的Simpson指数显著提高(P<0.05);250、500、1000 mg/kg MOP组的Chao1指数均显著增加(P<0.05)。各试验组盲肠弯曲杆菌门相对丰度均显著降低(P<0.05),1000、2000 mg/kg MOP组文昌鸡盲肠另枝菌属相对丰度显著降低(P<0.05),有益菌副拟杆菌属相对丰度显著提高(P<0.05);2000 mg/kg MOP组链球菌相对丰度显著降低(P<0.05);250、2000 mg/kg MOP组拟杆菌属相对丰度显著降低(P<0.05),CHKCI001菌属相对丰度显著提高(P<0.05)。研究表明,在文昌鸡的饲粮中添加适量MOP可改善肠道黏膜形态,提高十二指肠紧密连接蛋白Occludin mRNA表达量,降低炎症因子TNF-αmRNA表达量,增加盲肠微生物Alpha多样性和均匀度,增加肠道有益菌含量,促进肠道健康,MOP添加量以500 mg/kg为宜。

益肾固本法治疗稳定期慢性阻塞性肺疾病的用药经验

慢性阻塞性肺疾病(COPD)是一种以慢性、进展性气流受限和呼吸道症状为表现的肺部疾病,西医治疗效果尚不完全满意,病情反复加重和高致死率给个人和社会带来沉重的负担。中医药历史悠久,经验丰富,治疗COPD疗效较好。本病多属中医学“喘证”“肺胀”等范畴,曹世宏教授认为,COPD是由多种慢性肺系疾病反复发作,迁延不愈,导致肺气胀满,不能敛降而引起的一种病证。病机为本虚标实,多在久病肺虚的基础上损及五脏,其中尤以肺肾两脏为甚,并强调痰浊和血瘀为其发病的关键病理因素。曹教授治疗稳定期COPD主张衷中参西、取长补短,采用支气管舒张剂吸入等西医基础治疗的同时,联合中药补益肺肾之气,治病求本,其中党参、黄芪、巴戟天等是曹教授临床常用中药,有平补肺脾之气,温补肾阳之功。同时注意化痰祛瘀,因人制宜,临床疗效颇佳。本研究附验案1则以佐证。

基于药理学分析巴戟天治疗阿尔茨海默病的机制

目的:基于网络药理学、分子对接和GEO数据分析巴戟天治疗阿尔茨海默病(Alzheimer's disease,AD)的潜在靶点和作用机制。方法:利用中药系统药理学数据库与分析平台(TCMSP),获取巴戟天的主要活性成分,用SwissTargetPrediction获取巴戟天全部作用靶点。从DrugBank、PathCard、Chemogenomic Database和PubChem数据库获得AD相关靶点。使用韦恩图取交集,得到巴戟天治疗AD的共同作用靶点。利用Cytoscape3.8.0构建靶点的“成分-靶点”网络图,并分析靶点的相互作用PPI网络图、基因本体论(GO)和KEGG信号通路等。使用Autodock对关键成分和靶点进行分子对接,并用Pymol和Discovery Studio展示对接结果。最后利用GEO数据库Alzdata分析关键靶点基因在AD中的表达。结果:预测得到巴戟天的50种主要活性成分,636个作用靶点,674个AD相关靶点,其中巴戟天治疗AD共同靶点124个。GO富集分析得到蛋白质磷酸化、磷酸化的正调控、细胞对氮化合物的反应、水解酶活性的调节、细胞对化学刺激的反应。KEGG富集分析显示阿尔茨海默病为最显著的通路。分子对接显示,巴戟天的5个核心成分2-羟基-1,5-二甲氧基-6-(甲氧基甲基)-9,10-蒽醌、1-羟基-3-甲氧基-9,10-蒽醌、大黄素-A、甲基异茜草素、甲基异茜草素-1-甲醚和3个核心靶点EGFR、PARP1、FTO的结合较好,并且Egfr在AD病人中显著(P<0.05)上调表达,Parp1和Fto在AD病人中显著(P<0.05)下调表达。结论:巴戟天可能通过多个成分、多个靶点、多个通路,参与调控AD疾病进程。

基于DNA条形码ITS1、ITS2及其二级结构对药材巴戟天及易混伪品进行鉴定

[目的]利用DNA条形码ITS1、ITS2碱基序列及其二级结构对巴戟天药材及其混伪品进行鉴定.[方法]利用Codon Code Aligner 17.0或SeqMan软件对测序获得的双向序列进行拼接,从GenBnak上下载巴戟天及其混伪品的ITS1、ITS2碱基序列并利用MEGA 11.0软件分别与测序获得的碱基序列进行比对分析,获得测序样品的ITS1、ITS2碱基序列.将所有ITS1、ITS2碱基序列利用MEGA 11.0软件比对分析,分别构建NJ、ML系统聚类树并计算种内、种间遗传距离及各物种种间碱基变异位点、简约信息位点,利用在线网页构建各物种ITS1、ITS2碱基序列二级结构.[结果]基于ITS1碱基序列巴戟天与大果巴戟、虎刺、鸡眼藤、假巴戟、南五味子、香巴戟、羊角藤的种间遗传距离分别为0.034、0.233、0.052、0.052、1.004、1.004、0.049,种间简约信息位点分别为6、30、9、9、90、90、10个,根据NJ系统聚类树显示虎刺与羊角藤、香巴戟与南五味子无法鉴别,ML系统聚类树显示香巴戟与南五味子无法鉴别.基于ITS2碱基序列巴戟天与大果巴戟、虎刺、鸡眼藤、假巴戟、南五味子、香巴戟、羊角藤的种间遗传距离分别为0.014、0.113、0.034、0.015、0.509、0.516、0.017,种间简约信息位点分别为7、34、15、8、91、88、9个,根据NJ系统聚类树显示除香巴戟与南五味子无法鉴别外,其他各物种均独立聚为一支,ML系统聚类树显示各物种均独立聚为一支.基于ITS2构建的二级结构能够准确对各物种进行鉴别.[结论]基于ITS2构建的ML系统发育树、二级结构均能够对巴戟天及其混伪品进行鉴别,ITS2序列的物种鉴定能力优于ITS1序列.

巴戟天多糖对炎性微环境牙周膜成纤维细胞FN及FN-EDA表达的影响

目的:探讨巴戟天多糖(morinda officinalis polysaccharides,MOP)对炎性微环境牙周膜成纤维细胞纤连蛋白(fibronectin,FN)及含额外结构域A的纤连蛋白(fibronectin containing extra domain A,FN-EDA)表达的影响。方法:将36只大鼠随机分为对照组(n=12)和模型组(n=24),模型组采用正畸丝结扎法建立牙周炎,3周后每组各取6只大鼠通过Micro-CT确认建模完成;剩余模型组大鼠随机分为牙周炎组、生理盐水组(NS组)和MOP组。MOP组于大鼠左侧上颌第一磨牙腭侧注射MOP(200 mg/kg、3 d,50μL、4周),NS组注射等体积NS,牙周炎组不做任何处理。取大鼠左侧上颌骨组织,采用H-E染色观察牙周组织病理改变,免疫组织化学染色检测FN、FN-EDA的表达。体外培养牙周膜成纤维细胞,CCK-8法检测MOP对细胞活性的影响。将第4代细胞分为对照组、炎症组(10 mg/mL脂多糖)及实验组(12.5μg/mL MOP,12.5μg/mL MOP+10 mg/mL脂多糖)。利用qRT-PCR及Western印迹法检测FN和FN-EDA的表达变化。采用Prism 8.0软件包对数据进行统计学分析。结果:体内实验中,MOP组较牙周炎组和NS组FN-EDA表达显著下降(P<0.05),炎细胞浸润减少;但各组FN表达量未见统计学差异。体外实验中,与对照组相比,炎症组FN-EDA mRNA和蛋白表达量显著增高(P<0.0001);MOP显著降低炎症细胞FN-EDA表达,但对FN表达无明显影响。结论:FN-EDA在炎症牙周膜组织和细胞中表达升高,MOP可能通过下调FN-EDA而发挥炎症抑制作用。

阿里红化学成分及药理作用研究进展

阿里红为多孔菌科药用层孔菌的干燥菌体,具有良好的医用价值。本文总结了阿里红化学成分和药理学性质的研究概况,为阿里红的研究与开发提供参考依据。

巴戟天寡糖抗抑郁作用机制的研究进展

抑郁症是一种心理精神障碍疾病。近些年,全世界抑郁症发病率的不断升高,给社会带来沉重的经济负担。巴戟天寡糖(Morinda officinalis oligosaccharides,MOOs)是从中草药巴戟天中提取的活性物质,可以舒郁安神、补肾益智,同时对患者的认知障碍有一定的改善作用。本文基于抑郁症的假说机制,通过查阅现有的研究阐释MOOs与抑郁症之间的联系,提出MOOs通过调节单胺类神经递质水平、增强神经可塑性、调节HPA轴功能和细胞因子水平以及影响肠道菌群等机制改善抑郁症,为MOOs抗抑郁的研究提供新思路。

基于BMSCs淫羊藿-仙茅-巴戟天角药抗骨质疏松药效物质提取工艺研究

目的筛选淫羊藿-仙茅-巴戟天角药抗骨质疏松药效物质的最佳提取工艺。方法采用正交设计,选取提取时间、料液比、乙醇浓度为考察因素,以BMSCs细胞增殖率结合淫羊藿苷、总黄酮含量为考察指标,筛选淫羊藿-仙茅-巴戟天角药抗骨质疏松药效物质的最佳提取工艺,并对最佳提取条件进行验证。结果兼顾生产效率和节约能源,优选出淫羊藿-仙茅-巴戟天角药药效物质的最佳提取条件为提取时间1.5 h,乙醇浓度55%,溶剂用量10倍。验证实验结果BMSCs细胞增殖率、淫羊藿苷及总黄酮含量与工艺考察结果非常接近。结论该方法在保证淫羊藿-仙茅-巴戟天角药治疗骨质疏松的药效基础上,结合淫羊藿苷及总黄酮含量进行综合评价,优化提取工艺,使得实验结果更科学合理。

施用复合肥对巴戟天产量、养分吸收和寡糖累积量的影响

【目的】研究复合肥施用量对巴戟天产量、养分吸收和主要有效成分寡糖累积量的影响,为巴戟天的施肥管理提供理论依据。【方法】以巴戟天为研究对象,通过盆栽试验,设置每株施用复合肥0、2、4、6、8和10 g(CK、T1、T2、T3、T4和T5)共6个处理,测定巴戟天在不同施肥处理下的产量、养分含量和寡糖含量,分析各指标间的相关性,并运用主成分分析方法进行综合评价。【结果】施用复合肥可以显著提高巴戟天产量和N、P、K含量,并有利于提高寡糖的累积量。其中,单株施肥量为6 g处理的巴戟天产量和寡糖总累积量最高,与CK相比,分别提升114.47%和75.36%。施肥在一定程度上降低了巴戟天的寡糖含量,与CK相比,降幅最高达35.65%。巴戟天对复合肥中养分的利用效率表现为N>K>P,其中T3处理N、P、K累积量均高于其他处理。影响巴戟天寡糖累积量的最关键因素是产量,巴戟天产量与全株N/P/K含量、4种寡糖累积量呈显著或极显著正相关,但耐斯糖、1F−果呋喃糖基耐斯糖含量与产量、全株N/P/K含量呈显著或极显著负相关。主成分分析的综合得分由高到低为T3>T5>T4>T2>T1>CK,T3处理最佳。【结论】建议巴戟天的种植以N肥为主,适量补充K和P肥,适当施肥可显著提高巴戟天药材产量和寡糖累积量。