γ-mercapto-propyl trimethoxysilane （MPTS） and γ-methacryloxy propyltrimethoxysilane （MPTES） were self-assembled on a hy- droxylated glass substrate to form a two-dimensional organic monolayer （MPTS-MPTES SAM）....γ-mercapto-propyl trimethoxysilane （MPTS） and γ-methacryloxy propyltrimethoxysilane （MPTES） were self-assembled on a hy- droxylated glass substrate to form a two-dimensional organic monolayer （MPTS-MPTES SAM）. The terminal thiol groups （-SH） in the MPTS-MPTES SAM were in-situ oxidized into sulfonic acid groups （-SO3H） to endow the film with good chemisorption ability. Then rare earth （RE） （lanthanum-based） composite thin films were prepared by self-assembly technique based on the as-prepared SAM, taking advantage of the chemisorption ability of the sulfonic acid groups. Automatic force microscope （AFM）, X-ray photoelectron spectrometry （XPS）, contact angle measurement and ellipsometer were used to characterize MPTS-MPTES/RE composite films. The macrofriction and wear behaviors of the films sliding against an AISI-52100 steel ball were examined on a unidirectional friction and wear tester, and the worn surface morphologies were observed on an AFM. The results showed that MPTS-MPTES/RE films had a low friction coefficient （0.09） and a long wear life （5980 sliding pass） at a light load （50 mN）. It indicated that the superior tribological properties of the MPTS-MPTES/RE composite films were attributed to the special characteristic of RE elements, the mobility of the films and good bonding strength.展开更多
With the help of model experiments, we are able to offer a detailed proposal for the inhibition of DNA duplication and no inhibition of RNA viral infectivity. As a backbone, we introduced methyl phosphotriester (MPTE)...With the help of model experiments, we are able to offer a detailed proposal for the inhibition of DNA duplication and no inhibition of RNA viral infectivity. As a backbone, we introduced methyl phosphotriester (MPTE). Duplex formation according to the traditional Watson and Crick base-pairing: [(MPTE)<sub>n−1</sub> DNA] * DNA and [(MPTE)<sub>n−1</sub> DNA] * RNA, where n = number of DNA and RNA bases. However, in the latter case, inhibition is obtained by reduction of the number of MPTE linkages, as is confirmed with model experiments and under biological conditions with micro (mi)RNA substrates. The latter results have recently been published. One or more single MPTEs are disseminated over different places of DNA without neighbour MPTEs (Prof. Wen-Yih Chen and his group, Taiwan).展开更多
The work is to explore the relationship between the levels of cytokines(IL-1β and IL-6)in C57BL/6J mice treated with MPTP and brain lateralization.By using paw preference test,right-pawed,left-pawed mice models were ...The work is to explore the relationship between the levels of cytokines(IL-1β and IL-6)in C57BL/6J mice treated with MPTP and brain lateralization.By using paw preference test,right-pawed,left-pawed mice models were established.Following single injection of 1-methyl-4-phenyl-2,3,6-tetrahydropyrid(MPTP)(40 mg/kg)to impair dopaminergic neuron,enzyme linked immunosorbent assay(ELISA)kits were used for detection of plasma levels of cytokines.The results showed that in saline treated C57BL/6J mice(control),there was no obvious difference observed between left-pawed and right-pawed mice in plasma levels of IL-1β and IL-6.In MPTP treated mice,there was no difference between level of IL-1β in left-pawed mice and that in right-pawed ones in statistics,that is,they were increased on day 1 and day 3,but decreased on day 6.The plasma level of IL-6 was lower in left-pawed than that in right-pawed mice (p<0.005)after MPTP treatment. On day 1 and day 3,the level of IL-6 was almost the same as control;on day 6,it was significantly increased, higher than that of control(p<0.001)in left-pawed mice.While in right-pawed mice,on day 1 and day 3,it was no different from control,too.And on day 6,it significantly increased in compared with control(p<0.005).In conclusion,the level of plasma IL-6 of C57BL/6J mice treated with MPTP increased.The variation of IL-6 was correlated to brain lateralization.Cellular & Molecular Immunology.2004;1(3):219-223.展开更多
基金supported by the National Natural Science Foundation of China (50475023)
文摘γ-mercapto-propyl trimethoxysilane （MPTS） and γ-methacryloxy propyltrimethoxysilane （MPTES） were self-assembled on a hy- droxylated glass substrate to form a two-dimensional organic monolayer （MPTS-MPTES SAM）. The terminal thiol groups （-SH） in the MPTS-MPTES SAM were in-situ oxidized into sulfonic acid groups （-SO3H） to endow the film with good chemisorption ability. Then rare earth （RE） （lanthanum-based） composite thin films were prepared by self-assembly technique based on the as-prepared SAM, taking advantage of the chemisorption ability of the sulfonic acid groups. Automatic force microscope （AFM）, X-ray photoelectron spectrometry （XPS）, contact angle measurement and ellipsometer were used to characterize MPTS-MPTES/RE composite films. The macrofriction and wear behaviors of the films sliding against an AISI-52100 steel ball were examined on a unidirectional friction and wear tester, and the worn surface morphologies were observed on an AFM. The results showed that MPTS-MPTES/RE films had a low friction coefficient （0.09） and a long wear life （5980 sliding pass） at a light load （50 mN）. It indicated that the superior tribological properties of the MPTS-MPTES/RE composite films were attributed to the special characteristic of RE elements, the mobility of the films and good bonding strength.
文摘With the help of model experiments, we are able to offer a detailed proposal for the inhibition of DNA duplication and no inhibition of RNA viral infectivity. As a backbone, we introduced methyl phosphotriester (MPTE). Duplex formation according to the traditional Watson and Crick base-pairing: [(MPTE)<sub>n−1</sub> DNA] * DNA and [(MPTE)<sub>n−1</sub> DNA] * RNA, where n = number of DNA and RNA bases. However, in the latter case, inhibition is obtained by reduction of the number of MPTE linkages, as is confirmed with model experiments and under biological conditions with micro (mi)RNA substrates. The latter results have recently been published. One or more single MPTEs are disseminated over different places of DNA without neighbour MPTEs (Prof. Wen-Yih Chen and his group, Taiwan).
基金supported by INSERM.Shantou University and A French-Chinese Agreement(PRA B97-03 from the Association France-Chinese Pour-La Recherche Scientifique&Technique)the National Natural Science Foundation of China(#302712l1.#30371315)the Scientific and Developing Foundation of Shantou University(L0001l).
文摘The work is to explore the relationship between the levels of cytokines(IL-1β and IL-6)in C57BL/6J mice treated with MPTP and brain lateralization.By using paw preference test,right-pawed,left-pawed mice models were established.Following single injection of 1-methyl-4-phenyl-2,3,6-tetrahydropyrid(MPTP)(40 mg/kg)to impair dopaminergic neuron,enzyme linked immunosorbent assay(ELISA)kits were used for detection of plasma levels of cytokines.The results showed that in saline treated C57BL/6J mice(control),there was no obvious difference observed between left-pawed and right-pawed mice in plasma levels of IL-1β and IL-6.In MPTP treated mice,there was no difference between level of IL-1β in left-pawed mice and that in right-pawed ones in statistics,that is,they were increased on day 1 and day 3,but decreased on day 6.The plasma level of IL-6 was lower in left-pawed than that in right-pawed mice (p<0.005)after MPTP treatment. On day 1 and day 3,the level of IL-6 was almost the same as control;on day 6,it was significantly increased, higher than that of control(p<0.001)in left-pawed mice.While in right-pawed mice,on day 1 and day 3,it was no different from control,too.And on day 6,it significantly increased in compared with control(p<0.005).In conclusion,the level of plasma IL-6 of C57BL/6J mice treated with MPTP increased.The variation of IL-6 was correlated to brain lateralization.Cellular & Molecular Immunology.2004;1(3):219-223.
