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加味青蒿鳖甲汤对髓系MRD-L患者CD_(34)^+细胞源DC诱导过程中IL-12的影响 被引量:1
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作者 刘健 黄礼明 +7 位作者 唐志宇 宋益青 仇江辉 赵国静 马武开 严鲁萍 姚宇红 李秀军 《辽宁中医杂志》 CAS 2014年第3期400-402,共3页
目的:探讨加味青蒿鳖甲汤对树突细胞(DC)诱导和对IL-12分泌的影响。方法:本研究采用体外培养的方法,用含不同剂量(高、中、低)加味青蒿鳖甲汤动物血清联合细胞因子、不含药血清联合细胞因子及单纯用细胞因子,共同培养缓解期急性髓系白血... 目的:探讨加味青蒿鳖甲汤对树突细胞(DC)诱导和对IL-12分泌的影响。方法:本研究采用体外培养的方法,用含不同剂量(高、中、低)加味青蒿鳖甲汤动物血清联合细胞因子、不含药血清联合细胞因子及单纯用细胞因子,共同培养缓解期急性髓系白血病(AML-CR)患者骨髓中分离提取的CD+34细胞,诱导其成为DC,观察各组在不同阶段对DC成熟的影响,以及各组血清中IL-12的分泌变化。结果:含药血清均能上调DC表面特征性分子及共刺激分子CD80、CD83、CD86表达水平,较单纯细胞因子组比较有显著性差异(P<0.01),且含中剂量药血清更能促进DC分泌IL-12(P<0.01)。结论:联合细胞因子的含加味青蒿鳖甲汤的血清,可使来源AML-CR骨髓的CD+34,在体外比单纯用细胞因子诱导培养DC能够更好地促进DC的生长、成熟和分化,并能促进DC分泌IL-12,增加抗肿瘤作用。 展开更多
关键词 加味青蒿鳖甲汤 MRD—L CD34+ DC IL-12
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微小残留白血病的病因病机及治疗探讨 被引量:28
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作者 黄礼明 胡莉文 丘和明 《中医杂志》 CSCD 北大核心 2005年第6期465-466,共2页
温热毒邪是白血病的重要致病因素,正虚毒伏是微量残留白血病重要病理,伏毒积蓄是白血病复发的根本原因。骨髓、血液、脑脊液、睾丸等至阴之分为余毒伏藏之处。微小残留白血病以扶正祛毒为治疗原则,以祛除余毒为目标,扶正培本有助于祛毒... 温热毒邪是白血病的重要致病因素,正虚毒伏是微量残留白血病重要病理,伏毒积蓄是白血病复发的根本原因。骨髓、血液、脑脊液、睾丸等至阴之分为余毒伏藏之处。微小残留白血病以扶正祛毒为治疗原则,以祛除余毒为目标,扶正培本有助于祛毒,祛毒采取入阴搜剔,逐毒出阳的方法。 展开更多
关键词 微小残留白血病 骨髓移植 mrd-l 中医 清热解毒
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青蒿鳖甲汤对急性白血病缓解期患者CD34^+源DC诱导过程中IL-12的影响 被引量:2
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作者 肖洋 黄礼明 《北方药学》 2014年第2期77-77,共1页
目的:探讨青蒿鳖甲汤对AML-CR患者CD34+源DC诱导过程IL-12的影响。方法:选择贵州省人民医院AML-CR患者4例,免疫磁珠法提取CD34+细胞后以细胞因子扩增CD34+细胞,再以含药血清与细胞因子共诱导DC,观察DC形态,ILISA法分别检测第0、6、9天的... 目的:探讨青蒿鳖甲汤对AML-CR患者CD34+源DC诱导过程IL-12的影响。方法:选择贵州省人民医院AML-CR患者4例,免疫磁珠法提取CD34+细胞后以细胞因子扩增CD34+细胞,再以含药血清与细胞因子共诱导DC,观察DC形态,ILISA法分别检测第0、6、9天的IL-12水平。结果:各组IL-12水平随DC的逐渐成熟而逐渐升高,其中含药血清组与空白、因子组比较有显著差异(P<0.05),其中高剂量组与中低剂量组比较有显著差异(P<0.05),高剂量组与因子、空白组比较有显著差异(P<0.01),中剂量组与低剂量组、因子与空白组比较无显著差异(P>0.05)。结论:青蒿鳖甲汤在体外能从造血干细胞层面提高AML-CR患者IL-12水平,且以高剂量组效果最佳。 展开更多
关键词 AML-CR mrd-l IL-12 青蒿鳖甲汤 CD34+细胞 DC
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Low Flow Trends and Frequency Analysis in the Blue Nile Basin, Ethiopia
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作者 Kidist Assefa Mamaru A. Moges 《Journal of Water Resource and Protection》 2018年第2期182-203,共22页
Low flow analysis provides crucial information for the planning and design water resource development, risk assessment and environmental flow management. Understanding the low flow regimes and evaluating the magnitude... Low flow analysis provides crucial information for the planning and design water resource development, risk assessment and environmental flow management. Understanding the low flow regimes and evaluating the magnitudes for incorporating in water resources management is vital for the countries like Ethiopia where demand for water is increasing. However, there were hardly enough studies in understanding the trends of low flow and frequency analysis. Therefore, this study focuses on evaluation of the trends in low flows and regional low flow analysis in the Blue Nile Basin, Ethiopia. In order to carry out the study, 15 river sub-basins in the Blue Nile Basin were selected based on the long term data availability and presence of quality of data. The 3-day sustained low flow (3d-slf), the 7-day sustained low flow (7d-slf) and the 14-day sustained low flow (14d-slf) models were used to extract the data from the daily time series stream data obtained from MoWIE. Trends in low flow were analyzed separately by using Mann-Kendall (MK) trend test. Low flow frequency analysis was used to estimate the long term low flow quantiles. In addition, regional analysis for estimating the quantiles for ungaged catchments was also developed based on the regional growth curve and catchment characteristic of drainage basins. The results indicated that 3d-slf, 7d-slf and 14d-slf models of low flow series indicated no significant difference for each station at 95% CI. Out of the 15 selected stations, 12 of stations have indicated decreasing;two stations indicated increasing and remaining one station with no trend. Mainly decreasing trend was associated with the land cover and climate change which results in increasing runoff and evapotranspiration respectively. Weibull distribution—GEV and LGN was found best fit based on the L-Moment Ratio Diagram (L-MRD). Hence quantile estimations have indicated diminishing magnitudes of low flow quintiles for 2 - 500 years return periods. Regional low frequency analysis has provided a very good relationship between discharge and catchment characteristics with an R2 of 0.72. Where area (A) and rainfall (R) followed by slope were found sensitive to compute in developing the regional region equations between mean low flows and the physiographic data. This study indicated that there needs to be a new water management scenario and adaptation mechanism of climate change and land use land cover dynamics for utilizing water resource in the Blue Nile Basin. 展开更多
关键词 MANN-KENDALL Low Flow L-MRD PWM REGIONALIZATION BLUE NILE BASIN
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