Migration inhibitory factor-related protein 14 (MRP 14) is one of calcium-binding proteins,referred as S 100A9. The heterodimeric molecule formed by MRP 14 with its partner MRP8 (S 100A8) is the major fatty acid carri...Migration inhibitory factor-related protein 14 (MRP 14) is one of calcium-binding proteins,referred as S 100A9. The heterodimeric molecule formed by MRP 14 with its partner MRP8 (S 100A8) is the major fatty acid carrier in neutrophils.The MRP8/14 complex has been also implicated in the intracellular transport of arachidonic acid and its precursors in keratinocytes. We show here the involvement of MRP14 in human esophageal cancer. In an initial study,mRNA differential display - reverse transcription polymerase chain reaction (DD-PCR) was performed with two esophageal carcinomas,one esophageal adenocarcinoma and matched normal adjacent mucosa. DD-PCR with the arbitrary primer OPA3 showed that one cDNA band was highly expressed in normal tissues,but disappeared or substantially decreased in tumor counterparts. It was later identified to be the 3'-end of migration inhibitory factor-related protein 14 (MRP14).Northern blotting,RT-PCR and Western blotting corroborated the down-regulation of MRP14 in 58/64 squamous cell carcinomas and 2/2 adenocarcinomas as compared with adjacent normal epithelia of the esophagus. MRP14 was undetectable in 3/3 esophageal-carcinoma cell lines. Immunochemistry demonstrated that expression of MRP14 was restricted to normal esophageal epithelia. No mutation was found in the genomic DNA of the MRP14 gene by PCR and directed DNA sequencing. Our finding suggested that the reduction of MRP14 expression is a frequent event in Chinese human esophageal cancer.展开更多
目的探讨髓样相关蛋白8(MRP8)、MRP14及其异二聚体MRP8/14对小鼠骨髓源性树突状细胞(BMDC)表型成熟的影响。方法体外培养及纯化小鼠BMDC,分为对照组、1μg/m L MRP14处理组、1μg/m L MRP8处理组、1μg/m L MRP8/14处理组。采用流式细...目的探讨髓样相关蛋白8(MRP8)、MRP14及其异二聚体MRP8/14对小鼠骨髓源性树突状细胞(BMDC)表型成熟的影响。方法体外培养及纯化小鼠BMDC,分为对照组、1μg/m L MRP14处理组、1μg/m L MRP8处理组、1μg/m L MRP8/14处理组。采用流式细胞术检测刺激后BMDC表面共刺激分子CD40、CD80、CD86、主要组织相容性复合体Ⅱ(MHCⅡ)的表达情况。结果 MRP14、MRP8及MRP8/14均能促进BMDC表面共刺激分子CD40、CD80、CD86表达,MRP14、MRP8均能促进BMDC表面共刺激分子MHCⅡ表达,且MRP14和MRP8/14促进BMDC表达CD80、CD86的能力强于MRP8。结论 MRP14、MRP8及MRP8/14通过增加BMDC表面共刺激分子的表达而促进BMDC的表型成熟,且MRP8、MRP14及MRP8/14三者促进DC表达各种共刺激分子的能力不同。展开更多
Objective: Early and accurate evaluation of the presence and activity of synovitis is extremely important in the diagnosis and treatment of rheumatoid arthritis. Myeloid related protein 8/14 (MRP8/14), also known as c...Objective: Early and accurate evaluation of the presence and activity of synovitis is extremely important in the diagnosis and treatment of rheumatoid arthritis. Myeloid related protein 8/14 (MRP8/14), also known as calprotectin or S100A8/A9 is considered as a sensitive marker for local inflammatory activity in rheumatoid arthritis. The aim of this study is to demonstrate the efficacy of MRP8/14 as a marker of disease activity in RA. Methods: Thirty-one patients with diagnosis of RA who received treatment without biological drugs at our institution were included in this study. Serum MRP8/14, CRP and MMP-3 were tested in all patients. Disease activity was evaluated using DAS28-CRP and SDAI. Ultrasonography was performed on the wrists and MCP joints of both hands using semi-quantitative scale of power Doppler signal. The sum of scales in joints was calculated as the PD score. The correlation of MRP8/14 with serum biomarkers, disease activity and ultrasonography examination was investigated. Result: Serum MRP8/14 was strongly correlated with CRP (r = 0.63) and MMP-3 (r = 0.69). A correlation was observed between serum MRP8/14 and DAS28-CRP (r = 0.53) and SDAI (r = 0.66). No significant correlation was found between PD scores and MRP8/14. Conclusion: This study demonstrated that MRP8/14 is correlated with evaluated disease activity and markers of serum inflammatory response in patients not using biological drugs. MRP8/14 is considered an effective new method for objective evaluation of synovitis in RA.展开更多
基金supported by State Key Basic Research programme of China(G1998051205)National Key Technologies R&D Programme of China(2002BA711A06)+2 种基金The National High Technology Research and Development Program of China(2001AA221151)National Natural Science Foundation of China(30125026)Doctoral Program Fund of China(20010023016).
文摘Migration inhibitory factor-related protein 14 (MRP 14) is one of calcium-binding proteins,referred as S 100A9. The heterodimeric molecule formed by MRP 14 with its partner MRP8 (S 100A8) is the major fatty acid carrier in neutrophils.The MRP8/14 complex has been also implicated in the intracellular transport of arachidonic acid and its precursors in keratinocytes. We show here the involvement of MRP14 in human esophageal cancer. In an initial study,mRNA differential display - reverse transcription polymerase chain reaction (DD-PCR) was performed with two esophageal carcinomas,one esophageal adenocarcinoma and matched normal adjacent mucosa. DD-PCR with the arbitrary primer OPA3 showed that one cDNA band was highly expressed in normal tissues,but disappeared or substantially decreased in tumor counterparts. It was later identified to be the 3'-end of migration inhibitory factor-related protein 14 (MRP14).Northern blotting,RT-PCR and Western blotting corroborated the down-regulation of MRP14 in 58/64 squamous cell carcinomas and 2/2 adenocarcinomas as compared with adjacent normal epithelia of the esophagus. MRP14 was undetectable in 3/3 esophageal-carcinoma cell lines. Immunochemistry demonstrated that expression of MRP14 was restricted to normal esophageal epithelia. No mutation was found in the genomic DNA of the MRP14 gene by PCR and directed DNA sequencing. Our finding suggested that the reduction of MRP14 expression is a frequent event in Chinese human esophageal cancer.
文摘目的探讨髓样相关蛋白8(MRP8)、MRP14及其异二聚体MRP8/14对小鼠骨髓源性树突状细胞(BMDC)表型成熟的影响。方法体外培养及纯化小鼠BMDC,分为对照组、1μg/m L MRP14处理组、1μg/m L MRP8处理组、1μg/m L MRP8/14处理组。采用流式细胞术检测刺激后BMDC表面共刺激分子CD40、CD80、CD86、主要组织相容性复合体Ⅱ(MHCⅡ)的表达情况。结果 MRP14、MRP8及MRP8/14均能促进BMDC表面共刺激分子CD40、CD80、CD86表达,MRP14、MRP8均能促进BMDC表面共刺激分子MHCⅡ表达,且MRP14和MRP8/14促进BMDC表达CD80、CD86的能力强于MRP8。结论 MRP14、MRP8及MRP8/14通过增加BMDC表面共刺激分子的表达而促进BMDC的表型成熟,且MRP8、MRP14及MRP8/14三者促进DC表达各种共刺激分子的能力不同。
文摘Objective: Early and accurate evaluation of the presence and activity of synovitis is extremely important in the diagnosis and treatment of rheumatoid arthritis. Myeloid related protein 8/14 (MRP8/14), also known as calprotectin or S100A8/A9 is considered as a sensitive marker for local inflammatory activity in rheumatoid arthritis. The aim of this study is to demonstrate the efficacy of MRP8/14 as a marker of disease activity in RA. Methods: Thirty-one patients with diagnosis of RA who received treatment without biological drugs at our institution were included in this study. Serum MRP8/14, CRP and MMP-3 were tested in all patients. Disease activity was evaluated using DAS28-CRP and SDAI. Ultrasonography was performed on the wrists and MCP joints of both hands using semi-quantitative scale of power Doppler signal. The sum of scales in joints was calculated as the PD score. The correlation of MRP8/14 with serum biomarkers, disease activity and ultrasonography examination was investigated. Result: Serum MRP8/14 was strongly correlated with CRP (r = 0.63) and MMP-3 (r = 0.69). A correlation was observed between serum MRP8/14 and DAS28-CRP (r = 0.53) and SDAI (r = 0.66). No significant correlation was found between PD scores and MRP8/14. Conclusion: This study demonstrated that MRP8/14 is correlated with evaluated disease activity and markers of serum inflammatory response in patients not using biological drugs. MRP8/14 is considered an effective new method for objective evaluation of synovitis in RA.
