目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2...目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2位研究人员按照纳入与排除标准对所获文献独立进行筛选、提取,采用纽卡斯尔-渥太华量表评分(NOS)进行文献质量评价。以感染率为结局指标合并数据,采用R(4.3.1)进行Meta分析,并根据骨髓炎类型、患者年龄、研究地区进行亚组分析。结果:共纳入26篇文献,共包含20860名骨髓炎患者。Meta分析结果显示,骨髓炎患者MRSA感染率为0.240(95%CI:0.183~0.297)。亚组分析结果显示,椎体骨髓炎(VO)患者、18~60岁、北美地区骨髓炎患者的MRSA感染率更高。结论:骨髓炎患者中MRSA的感染率较高,需采取有效的预防和控制措施减少MRSA的传播。展开更多
Objective:To synthesize zinc oxide nanoparticles(ZnONPs)and evaluate their antibacterial and wound healing effects against wounds infected with methicillin-resistant Staphylococcus aureus(MRSA).Methods:ZnONPs were pre...Objective:To synthesize zinc oxide nanoparticles(ZnONPs)and evaluate their antibacterial and wound healing effects against wounds infected with methicillin-resistant Staphylococcus aureus(MRSA).Methods:ZnONPs were prepared by sol-gel method and characterized by X-ray diffraction(XRD)analysis and scanning electron microscopy(SEM).A total of 18 rabbits were divided into three groups:the ZnONPs group,the gentamicin group and the control group.A wound of 3 cm^(2) was inflicted on each rabbit and contaminated with MRSA inoculum.Treatment was started from the fourth day post-surgery.Wound healing,microbiological analysis,and histopathological analysis were performed to assess the efficacy of ZnONPs ointment.Results:XRD analysis confirmed the hexagonal wurtzite structure of the ZnONPs with an average crystallite size of 29.23 nm.SEM revealed discoid-shaped ZnONPs with a rough surface and an average size of 48.36 nm.Energy-dispersive X-ray analysis confirmed the purity of ZnONPs.Moreover,the particle size ranged from 100-700 nm with a high agglomeration trend.Treatment with ZnONPs promoted MRSA-infected wound healing.In addition,ZnONPs showed a good antibacterial effect as evidenced by a dose-dependent increase in the zone of inhibition.Conclusions:ZnONPs accelerate the healing of MRSA-infected wounds.Therefore,it can be explored for the treatment of MRSA infection.展开更多
Surgical Site Infections (SSIs) remain a prevalent issue in healthcare. An average of 2% to 4% of all Total Knee Arthroplasties (TKA/TKR) result in a Prosthetic Joint Infection (PJI) (Ashraf et al. , 2018). These surg...Surgical Site Infections (SSIs) remain a prevalent issue in healthcare. An average of 2% to 4% of all Total Knee Arthroplasties (TKA/TKR) result in a Prosthetic Joint Infection (PJI) (Ashraf et al. , 2018). These surgical site infections cause significant distress to the patient and require extended courses of antibiotic treatment and revision surgery of the infected joint. SSIs also reduce financial reimbursement to the surgery facility and affect the performing surgeon’s performance scores. To prevent surgical infection, healthcare facilities have implemented various screening or decolonization methods to prevent surgical infection to may cause infection. Various treatment methods exist for managing MRSA preoperatively which include Povidone Iodine (PI) application as a universal decolonization method and/or screening every patient pre-operatively for MRSA and treating MRSA-positive patients with Mupirocin ointment. Both interventions are well-established in the literature. At the author’s facility, the elective TKR populations were analyzed while each intervention was implemented. In 2019 TKR patients underwent MRSA swabbing and testing and in 2021 PI decolonization was the decolonization method of choice. The study revealed that MRSA testing and swabbing were better at reducing SSI related to MRSA than Povidone Iodine decolonization.展开更多
文摘目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2位研究人员按照纳入与排除标准对所获文献独立进行筛选、提取,采用纽卡斯尔-渥太华量表评分(NOS)进行文献质量评价。以感染率为结局指标合并数据,采用R(4.3.1)进行Meta分析,并根据骨髓炎类型、患者年龄、研究地区进行亚组分析。结果:共纳入26篇文献,共包含20860名骨髓炎患者。Meta分析结果显示,骨髓炎患者MRSA感染率为0.240(95%CI:0.183~0.297)。亚组分析结果显示,椎体骨髓炎(VO)患者、18~60岁、北美地区骨髓炎患者的MRSA感染率更高。结论:骨髓炎患者中MRSA的感染率较高,需采取有效的预防和控制措施减少MRSA的传播。
文摘Objective:To synthesize zinc oxide nanoparticles(ZnONPs)and evaluate their antibacterial and wound healing effects against wounds infected with methicillin-resistant Staphylococcus aureus(MRSA).Methods:ZnONPs were prepared by sol-gel method and characterized by X-ray diffraction(XRD)analysis and scanning electron microscopy(SEM).A total of 18 rabbits were divided into three groups:the ZnONPs group,the gentamicin group and the control group.A wound of 3 cm^(2) was inflicted on each rabbit and contaminated with MRSA inoculum.Treatment was started from the fourth day post-surgery.Wound healing,microbiological analysis,and histopathological analysis were performed to assess the efficacy of ZnONPs ointment.Results:XRD analysis confirmed the hexagonal wurtzite structure of the ZnONPs with an average crystallite size of 29.23 nm.SEM revealed discoid-shaped ZnONPs with a rough surface and an average size of 48.36 nm.Energy-dispersive X-ray analysis confirmed the purity of ZnONPs.Moreover,the particle size ranged from 100-700 nm with a high agglomeration trend.Treatment with ZnONPs promoted MRSA-infected wound healing.In addition,ZnONPs showed a good antibacterial effect as evidenced by a dose-dependent increase in the zone of inhibition.Conclusions:ZnONPs accelerate the healing of MRSA-infected wounds.Therefore,it can be explored for the treatment of MRSA infection.
文摘Surgical Site Infections (SSIs) remain a prevalent issue in healthcare. An average of 2% to 4% of all Total Knee Arthroplasties (TKA/TKR) result in a Prosthetic Joint Infection (PJI) (Ashraf et al. , 2018). These surgical site infections cause significant distress to the patient and require extended courses of antibiotic treatment and revision surgery of the infected joint. SSIs also reduce financial reimbursement to the surgery facility and affect the performing surgeon’s performance scores. To prevent surgical infection, healthcare facilities have implemented various screening or decolonization methods to prevent surgical infection to may cause infection. Various treatment methods exist for managing MRSA preoperatively which include Povidone Iodine (PI) application as a universal decolonization method and/or screening every patient pre-operatively for MRSA and treating MRSA-positive patients with Mupirocin ointment. Both interventions are well-established in the literature. At the author’s facility, the elective TKR populations were analyzed while each intervention was implemented. In 2019 TKR patients underwent MRSA swabbing and testing and in 2021 PI decolonization was the decolonization method of choice. The study revealed that MRSA testing and swabbing were better at reducing SSI related to MRSA than Povidone Iodine decolonization.
